##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146588_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3983768
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.121547489713258	31.0	31.0	34.0	28.0	34.0
2	31.35470790467718	31.0	31.0	34.0	28.0	34.0
3	31.49050647527667	33.0	31.0	34.0	28.0	34.0
4	34.98317020469064	37.0	35.0	37.0	32.0	37.0
5	35.02237580100046	37.0	35.0	37.0	32.0	37.0
6	35.091898925841065	37.0	35.0	37.0	32.0	37.0
7	34.99170107295405	37.0	35.0	37.0	32.0	37.0
8	34.994539089625704	37.0	35.0	37.0	32.0	37.0
9	36.61186444592155	39.0	37.0	39.0	32.0	39.0
10-11	36.594616955605844	39.0	37.0	39.0	32.0	39.0
12-13	36.54343990413096	39.0	37.0	39.0	32.0	39.0
14-15	37.895958298776435	40.0	38.0	41.0	32.0	41.0
16-17	37.927433650754764	40.0	38.0	41.0	32.0	41.0
18-19	37.94705276512086	40.0	38.0	41.0	32.0	41.0
20-21	38.039259941844	40.0	38.0	41.0	32.0	41.0
22-23	38.052606477084005	40.0	38.0	41.0	32.0	41.0
24-25	38.077690267103904	40.0	38.0	41.0	32.0	41.0
26-27	38.14083438091777	40.0	38.0	41.0	32.0	41.0
28-29	38.11786743103514	40.0	38.0	41.0	32.0	41.0
30-31	38.081949927807045	40.0	38.0	41.0	32.0	41.0
32-33	38.04996751818881	40.0	38.0	41.0	32.0	41.0
34-35	38.00274049593249	40.0	38.0	41.0	32.0	41.0
36-37	37.93274959786815	40.0	38.0	41.0	32.0	41.0
38-39	37.875561779702025	40.0	38.0	41.0	31.0	41.0
40-41	37.82238473726382	40.0	38.0	41.0	31.0	41.0
42-43	37.675596445375334	40.0	37.5	41.0	31.0	41.0
44-45	37.56653951736145	40.0	37.0	41.0	31.0	41.0
46-47	37.45650826052119	40.0	37.0	41.0	30.5	41.0
48-49	37.35350602745943	40.0	37.0	41.0	30.0	41.0
50-51	37.2230785025634	40.0	36.0	41.0	30.0	41.0
52-53	37.09275100859287	40.0	36.0	41.0	30.0	41.0
54-55	36.97318995483673	39.5	35.5	41.0	30.0	41.0
56-57	36.78381660277405	39.0	35.0	41.0	30.0	41.0
58-59	36.58765093750439	39.0	35.0	41.0	30.0	41.0
60-61	36.37120371969452	39.0	35.0	41.0	30.0	41.0
62-63	36.13562725038205	38.0	35.0	40.0	29.5	41.0
64-65	35.84395501946901	37.5	35.0	40.0	29.0	41.0
66-67	35.5997721252844	37.0	35.0	40.0	29.0	41.0
68-69	35.337030419442094	36.5	34.0	39.5	29.0	41.0
70-71	35.054082341140344	36.0	34.0	39.0	29.0	41.0
72-73	34.71787626689104	35.5	34.0	39.0	28.0	41.0
74-75	34.38717339965581	35.0	34.0	38.5	28.0	40.0
76-77	34.112717909275844	35.0	34.0	37.0	28.0	39.5
78-79	33.79388182243544	35.0	34.0	37.0	27.0	39.0
80-81	33.54886215763568	35.0	34.0	36.0	27.0	39.0
82-83	33.27672620493965	35.0	33.0	36.0	27.0	37.5
84-85	33.07409191499103	35.0	33.0	36.0	27.0	37.0
86-87	32.86307925059893	35.0	33.0	35.0	26.5	37.0
88-89	32.67558816176042	35.0	33.0	35.0	26.0	36.0
90-91	32.52021779380727	35.0	33.0	35.0	26.0	36.0
92-93	32.36242120525091	35.0	33.0	35.0	25.5	36.0
94-95	32.232334312640695	35.0	33.0	35.0	25.0	36.0
96-97	32.129590126734286	35.0	33.0	35.0	25.0	35.0
98-99	32.00614581471612	35.0	33.0	35.0	25.0	35.0
100	30.852669633372223	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39753367161770115
1101	2	0.4325649109989449
1101	3	0.43121430656783133
1101	4	0.4408348350129714
1101	5	0.3880854707446062
1101	6	0.3590054591441856
1101	7	0.3870394374040984
1101	8	0.47704327476748176
1101	9	0.5496081429858322
1101	10-11	0.6433346837138245
1101	12-13	0.6197302537248959
1101	14-15	0.7156699363135388
1101	16-17	0.6692426184119498
1101	18-19	0.6947879794360077
1101	20-21	0.6606385827073495
1101	22-23	0.6805959460291575
1101	24-25	0.6555816913225385
1101	26-27	0.6601929714064383
1101	28-29	0.6532196315548404
1101	30-31	0.6604513149533702
1101	32-33	0.725129167939329
1101	34-35	0.6528689871265101
1101	36-37	0.642105029851642
1101	38-39	0.6525447496899872
1101	40-41	0.6486049299840673
1101	42-43	0.6561966978938898
1101	44-45	0.585579059923198
1101	46-47	0.5569469977718455
1101	48-49	0.5549632768163733
1101	50-51	0.5230271062545668
1101	52-53	0.5660078409064297
1101	54-55	0.615159343025006
1101	56-57	0.6298681200861935
1101	58-59	0.6136732558052245
1101	60-61	0.6363055734247283
1101	62-63	0.6157530666823803
1101	64-65	0.5576289673061083
1101	66-67	0.511999570528765
1101	68-69	0.4770865033625853
1101	70-71	0.4983449888698672
1101	72-73	0.5079458374653427
1101	74-75	0.5403029927790897
1101	76-77	0.47762200630918983
1101	78-79	0.5119549121387408
1101	80-81	0.4810987088040761
1101	82-83	0.50562712519082
1101	84-85	0.43969253060569713
1101	86-87	0.4608337665260578
1101	88-89	0.47103996551354044
1101	90-91	0.46388436447008274
1101	92-93	0.5223627589144613
1101	94-95	0.5052612042813536
1101	96-97	0.5465826572533174
1101	98-99	0.5120086629196408
1101	100	0.5352217788089746
1102	1	0.22242741297534963
1102	2	0.3560209865093924
1102	3	0.3433459220207702
1102	4	0.32084760194403117
1102	5	0.31211942176232554
1102	6	0.37247826768789594
1102	7	0.3807982784299284
1102	8	0.3742469694153101
1102	9	0.520085631382301
1102	10-11	0.48485652111693867
1102	12-13	0.45212432682315296
1102	14-15	0.5966987905387668
1102	16-17	0.5852604041441154
1102	18-19	0.5178964758027575
1102	20-21	0.5125064394264598
1102	22-23	0.5495700673046429
1102	24-25	0.5573296963509478
1102	26-27	0.5706093308203108
1102	28-29	0.5620978788703326
1102	30-31	0.5633771931973612
1102	32-33	0.5231445643998427
1102	34-35	0.5471477460802774
1102	36-37	0.5300917136542296
1102	38-39	0.5070299566462282
1102	40-41	0.519358141953937
1102	42-43	0.5556285251063784
1102	44-45	0.4424163539991426
1102	46-47	0.47483068119778693
1102	48-49	0.49758220536259756
1102	50-51	0.55372137373989
1102	52-53	0.4216932465743781
1102	54-55	0.4370592501376436
1102	56-57	0.4679114862535343
1102	58-59	0.47174711993092444
1102	60-61	0.4564858628947448
1102	62-63	0.4122954424330487
1102	64-65	0.4959273948740943
1102	66-67	0.4105093991066582
1102	68-69	0.3513165047634743
1102	70-71	0.3578917443358236
1102	72-73	0.3832507523641766
1102	74-75	0.43892029052913273
1102	76-77	0.36092728492139514
1102	78-79	0.33173805542813994
1102	80-81	0.2532840606912288
1102	82-83	0.2897767252354697
1102	84-85	0.3021680474095163
1102	86-87	0.25384801159717085
1102	88-89	0.2561008634790056
1102	90-91	0.2238978299969645
1102	92-93	0.23983469587672346
1102	94-95	0.2654171796597353
1102	96-97	0.2074643960694189
1102	98-99	0.26615547597354805
1102	100	0.2264267161441893
1103	1	0.18396520115744508
1103	2	0.23583360874761894
1103	3	0.27091273724909115
1103	4	0.23043966598394405
1103	5	0.1953718048346289
1103	6	0.2698294184154548
1103	7	0.2600210725561567
1103	8	0.31806844699517
1103	9	0.3913399102413635
1103	10-11	0.43879626027536034
1103	12-13	0.4066937022806343
1103	14-15	0.5160912759201963
1103	16-17	0.511743227849287
1103	18-19	0.5096643094537257
1103	20-21	0.4452210159757257
1103	22-23	0.45623779274404086
1103	24-25	0.5025061112297919
1103	26-27	0.4495113668319206
1103	28-29	0.4348618467503371
1103	30-31	0.47302365481226616
1103	32-33	0.4737160307250434
1103	34-35	0.473703412515178
1103	36-37	0.4792051790757199
1103	38-39	0.5405832562655704
1103	40-41	0.446777604364172
1103	42-43	0.43596209270359765
1103	44-45	0.44511987179236456
1103	46-47	0.4875441864691439
1103	48-49	0.40460351503944736
1103	50-51	0.45935700419319403
1103	52-53	0.4402165587489293
1103	54-55	0.3733408278257144
1103	56-57	0.4262982792726362
1103	58-59	0.3916203256446238
1103	60-61	0.35894705095446255
1103	62-63	0.40621125082686405
1103	64-65	0.44537714568735254
1103	66-67	0.4464389973899401
1103	68-69	0.3856794112029789
1103	70-71	0.2786304133834818
1103	72-73	0.30943011680041366
1103	74-75	0.32650092816042076
1103	76-77	0.27167336954651944
1103	78-79	0.2622377544699077
1103	80-81	0.20127122536456454
1103	82-83	0.2686274274435547
1103	84-85	0.24579329772173253
1103	86-87	0.2866240094696195
1103	88-89	0.2653930530710227
1103	90-91	0.3019985751395353
1103	92-93	0.299662962107206
1103	94-95	0.3780795595473805
1103	96-97	0.40737610943045865
1103	98-99	0.3682918208900219
1103	100	0.4463175541753053
1104	1	0.06751350324664784
1104	2	0.17813000833220727
1104	3	0.20294170719392923
1104	4	0.19244482025411003
1104	5	0.12378186603640273
1104	6	0.1949246863015901
1104	7	0.20231306279126926
1104	8	0.237725301043632
1104	9	0.34709299600788057
1104	10-11	0.29737824201338725
1104	12-13	0.28595021592730774
1104	14-15	0.4174089322005159
1104	16-17	0.4142440811880945
1104	18-19	0.44142119659823464
1104	20-21	0.36359280402400174
1104	22-23	0.44845723621151024
1104	24-25	0.4701355204495883
1104	26-27	0.3921204411694035
1104	28-29	0.4260237596195111
1104	30-31	0.4820957616824515
1104	32-33	0.4683032300203678
1104	34-35	0.4262356630160298
1104	36-37	0.4119965774205028
1104	38-39	0.4209305003183985
1104	40-41	0.38434458757026135
1104	42-43	0.42482504856549497
1104	44-45	0.3692516319735475
1104	46-47	0.38727512537008124
1104	48-49	0.353461999302219
1104	50-51	0.3727647725332375
1104	52-53	0.28310093211811704
1104	54-55	0.22254282635039857
1104	56-57	0.2791202240343793
1104	58-59	0.3231391079171715
1104	60-61	0.29566601250596847
1104	62-63	0.25175485099327943
1104	64-65	0.28972043014434234
1104	66-67	0.2739598354568429
1104	68-69	0.2582242465059963
1104	70-71	0.21799842132238467
1104	72-73	0.22701327972535523
1104	74-75	0.23047268861134995
1104	76-77	0.28704851181288404
1104	78-79	0.30200308370306317
1104	80-81	0.2403352086567594
1104	82-83	0.2667795857941897
1104	84-85	0.23897806450864323
1104	86-87	0.16224280979456296
1104	88-89	0.19608145108691133
1104	90-91	0.17213402516811982
1104	92-93	0.09313026444644379
1104	94-95	0.1428102883557898
1104	96-97	0.20606051516946167
1104	98-99	0.27571532386400577
1104	100	0.3353920680315561
1105	1	0.2051455184254607
1105	2	0.23697240487742732
1105	3	0.1934957188220885
1105	4	0.29089471158818725
1105	5	0.26369279939943624
1105	6	0.3472465326899581
1105	7	0.33483471306156787
1105	8	0.3642504256199004
1105	9	0.3831424247686499
1105	10-11	0.3996588654360451
1105	12-13	0.3670644500061755
1105	14-15	0.5103190357851446
1105	16-17	0.48280923099563466
1105	18-19	0.4635022351604121
1105	20-21	0.438920256735706
1105	22-23	0.4621197942443729
1105	24-25	0.49921815849411644
1105	26-27	0.47617495998866843
1105	28-29	0.49362820312668987
1105	30-31	0.49508190325666135
1105	32-33	0.5347950914961928
1105	34-35	0.4650571211106609
1105	36-37	0.4936904204772361
1105	38-39	0.42503458682060824
1105	40-41	0.4348926418149901
1105	42-43	0.49045451308307975
1105	44-45	0.4426786199217432
1105	46-47	0.46962119865966656
1105	48-49	0.5134743771430053
1105	50-51	0.4637299187112731
1105	52-53	0.45776697134048305
1105	54-55	0.4184251448739147
1105	56-57	0.4102744138834211
1105	58-59	0.4423590223486258
1105	60-61	0.45010837763724965
1105	62-63	0.38840668260708355
1105	64-65	0.46219990001231537
1105	66-67	0.46074955671763007
1105	68-69	0.4199428327287933
1105	70-71	0.3890894047099138
1105	72-73	0.40510745832295925
1105	74-75	0.4275254421177337
1105	76-77	0.42628309129482744
1105	78-79	0.3682426324688137
1105	80-81	0.31983286105970876
1105	82-83	0.3842049949106112
1105	84-85	0.4135142709444892
1105	86-87	0.3669327630303556
1105	88-89	0.3806902506178318
1105	90-91	0.4049977712984827
1105	92-93	0.4909859036542912
1105	94-95	0.4355711601112233
1105	96-97	0.3976445846756036
1105	98-99	0.4094965664915833
1105	100	0.51082324304409
1106	1	0.088290331629576
1106	2	0.08695402562908683
1106	3	0.13663366075256178
1106	4	0.11107564524213842
1106	5	0.10551314421163482
1106	6	0.16114076993928705
1106	7	0.19583344393703328
1106	8	0.20004691433086208
1106	9	0.22343910839305892
1106	10-11	0.2302470354871602
1106	12-13	0.24336171429323628
1106	14-15	0.28415343631100853
1106	16-17	0.3138405047806003
1106	18-19	0.32837190708749375
1106	20-21	0.33864761515507524
1106	22-23	0.3685685305246267
1106	24-25	0.3980647364767691
1106	26-27	0.3154835977608954
1106	28-29	0.29822919846788665
1106	30-31	0.2870831713432409
1106	32-33	0.29527886237524825
1106	34-35	0.3109064284747092
1106	36-37	0.30032370546649645
1106	38-39	0.2589049688583813
1106	40-41	0.25836291970122005
1106	42-43	0.2733563702694255
1106	44-45	0.3338803054448718
1106	46-47	0.2835060498144486
1106	48-49	0.2748518287782957
1106	50-51	0.27446114589198345
1106	52-53	0.2224040371613185
1106	54-55	0.12641242960724952
1106	56-57	0.17561634130088777
1106	58-59	0.1943293385651117
1106	60-61	0.2554054415986613
1106	62-63	0.20723950321450246
1106	64-65	0.2729577730254178
1106	66-67	0.24723780652831806
1106	68-69	0.17027018204379374
1106	70-71	0.151394997265335
1106	72-73	0.22626776347631505
1106	74-75	0.2404353256752927
1106	76-77	0.2557157782430721
1106	78-79	0.21021192407371103
1106	80-81	0.20612756159361822
1106	82-83	0.1569620704763821
1106	84-85	0.1948233241959727
1106	86-87	0.20281856050512204
1106	88-89	0.2505989801157824
1106	90-91	0.22692907072097057
1106	92-93	0.19503516567445445
1106	94-95	0.13859191079416178
1106	96-97	0.1459020109966147
1106	98-99	0.25369793958972764
1106	100	0.27459333325290913
1107	1	0.07676025638695094
1107	2	0.1567483720882592
1107	3	0.16085704986942062
1107	4	0.17211451165372438
1107	5	0.18468223548222085
1107	6	0.24234999885511854
1107	7	0.2001653553406726
1107	8	0.26628547970453553
1107	9	0.35499421128923814
1107	10-11	0.31798412219162486
1107	12-13	0.33315703302179855
1107	14-15	0.34634137432671963
1107	16-17	0.35691285261950867
1107	18-19	0.32980009198308124
1107	20-21	0.3409684513135929
1107	22-23	0.3367945299162969
1107	24-25	0.3467435511981236
1107	26-27	0.3169039707975756
1107	28-29	0.34957427632413385
1107	30-31	0.33981640367366595
1107	32-33	0.3434914847797188
1107	34-35	0.31823399497098137
1107	36-37	0.34246258615079483
1107	38-39	0.2656471743440889
1107	40-41	0.23941015037152624
1107	42-43	0.28897816010596955
1107	44-45	0.19714906769481644
1107	46-47	0.29267394459958496
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2216	62-63	-0.5885816404598287
2216	64-65	-0.6060082273438496
2216	66-67	-0.5238337353595099
2216	68-69	-0.5862917050187946
2216	70-71	-0.5768921818186001
2216	72-73	-0.5330302791098518
2216	74-75	-0.5348055535084484
2216	76-77	-0.5673751009384134
2216	78-79	-0.508266503357234
2216	80-81	-0.5451939729083648
2216	82-83	-0.5017547095688357
2216	84-85	-0.523162479872262
2216	86-87	-0.5114788457447617
2216	88-89	-0.5202606683506161
2216	90-91	-0.5456333484843405
2216	92-93	-0.5068197059491659
2216	94-95	-0.5656496743088866
2216	96-97	-0.4998986355463799
2216	98-99	-0.490650815129797
2216	100	-0.662054469839628
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1159.0
1	279.0
2	228.0
3	193.0
4	185.0
5	199.0
6	326.0
7	867.0
8	2522.0
9	3706.0
10	3588.0
11	4197.0
12	4946.0
13	6246.0
14	8558.0
15	9916.0
16	10803.0
17	11887.0
18	13254.0
19	15086.0
20	16958.0
21	19084.0
22	20784.0
23	24950.0
24	29992.0
25	36411.0
26	38552.0
27	35828.0
28	38761.0
29	47627.0
30	55554.0
31	64789.0
32	82582.0
33	110480.0
34	162472.0
35	249364.0
36	461067.0
37	946302.0
38	1099515.0
39	343996.0
40	555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37845908299186	20.170073360249177	12.942370142107215	24.509097414651748
2	15.105269384579948	20.883273673272186	40.40606686431391	23.605390077833963
3	17.742321239253755	26.411243926581353	30.488586746255457	25.35784808790944
4	12.284905681708267	17.595671797216614	39.880615163532354	30.23880735754276
5	13.180822276415697	38.517742863825625	35.53778286494109	12.763651994817593
6	29.51067466559934	39.053112902889445	18.397394392154233	13.03881803935698
7	25.955999753781526	33.16253811047421	23.56644946679882	17.315012668945442
8	24.76517886103488	36.077697437563394	21.21783135690376	17.939292344497968
9	25.78614930280276	15.934652265526793	21.10989862329042	37.16929980838003
10-11	24.064626555312955	26.889306260141705	29.124746355266172	19.921320829279164
12-13	24.520340381235826	24.79178326368493	28.47042965645369	22.217446698625555
14-15	22.28887006676606	26.163017308830295	25.66951648902957	25.878596135374078
16-17	21.97251269095789	28.89067845013612	26.623056474307255	22.513752384598735
18-19	21.895617032833	28.73975907912544	27.80964442201274	21.55497946602882
20-21	23.052753570617995	27.87286963352252	27.15405483515679	21.920321960702697
22-23	23.09802599987163	27.61889413939626	26.7738361360207	22.509243724711407
24-25	22.83535798012969	27.790312142956914	27.15023468753046	22.22409518938294
26-27	22.58904814147977	28.01128381675353	26.82197405249663	22.577693989270074
28-29	22.376436362905537	28.295469961362024	26.828495631246014	22.499598044486426
30-31	22.764797101786073	27.94519119707716	26.841051079889933	22.448960621246826
32-33	22.781963368792777	28.24751364336855	26.414747349586285	22.55577563825238
34-35	22.999256576185363	28.062327004474362	26.795642365660505	22.142774053679773
36-37	22.931560336297274	27.750881639081776	26.878444290321525	22.439113734299426
38-39	22.73619474219294	27.81926164179624	27.08618207321726	22.358361542793563
40-41	22.730893324950554	27.440867806046278	27.32817763116987	22.5000612378333
42-43	22.58184385473878	27.60010954388539	27.054864904249598	22.76318169712623
44-45	22.430772257223193	28.0419261276795	27.080023008393994	22.44727860670331
46-47	22.553673846459958	27.71660314584758	26.941804495392297	22.787918512300166
48-49	22.73231431606905	27.505338151163677	27.04907807676561	22.71326945600166
50-51	22.591266178831702	27.57391860464678	26.912815171046866	22.92200004547465
52-53	22.359396054348373	27.75592979645615	26.96402562093172	22.920648528263754
54-55	22.369442350481748	27.678285762450795	26.929019082075143	23.023252804992314
56-57	22.71646205573062	27.59946171855096	26.757856763467213	22.926219462251204
58-59	22.40332231623413	27.449662979280248	27.20447933778808	22.942535366697538
60-61	22.380492339776005	27.808981212193544	26.919716832392165	22.89080961563829
62-63	22.490672203146534	27.55689802182489	26.823413269485926	23.12901650554266
64-65	22.146326963458463	27.561185448513214	26.95638529011437	23.336102297913953
66-67	22.46226367091923	27.41242074727009	26.9083818654552	23.216933716355477
68-69	22.247673832663693	27.465326560438264	27.09375789517457	23.193241711723473
70-71	22.282362137633523	27.394926395050394	26.96420052949983	23.358510937816245
72-73	22.5045026166434	27.42847322098903	27.0612913665341	23.005732795833474
74-75	22.407060118019576	27.723167469624766	26.947744507933702	22.92202790442196
76-77	22.44036410946802	27.516029289163402	26.824818802071004	23.218787799297573
78-79	22.413657281155615	27.58376079554899	26.815340217708272	23.18724170558712
80-81	22.34110061823606	27.573964006396817	27.118475206169773	22.966460169197347
82-83	22.62554337918246	27.093091145477675	27.445311544936366	22.83605393040349
84-85	22.806287537431427	27.42717400658705	26.75052813882183	23.01601031715969
86-87	22.291585530398773	27.588512863291566	27.031568394418997	23.08833321189066
88-89	22.63383538142556	27.11984329506498	26.900419199771658	23.3459021237378
90-91	22.598876941316494	27.053585932130293	27.046550640469086	23.30098648608413
92-93	22.841439675029445	27.09003320456181	26.955591913523424	23.112935206885325
94-95	22.309179582659105	27.087222661412795	27.20650132136133	23.397096434566766
96-97	22.52847182965894	27.078173481565226	26.996859493750392	23.396495195025437
98-99	22.421988172430733	27.397645793574117	26.74278240614521	23.437583627849943
100	22.83370612185945	27.03578644036151	26.515216138081975	23.615291299697063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2631.0
1	1842.0
2	1107.0
3	1157.0
4	1091.5
5	956.0
6	843.5
7	788.5
8	758.5
9	723.5
10	670.5
11	632.5
12	632.0
13	664.0
14	734.0
15	903.5
16	1061.5
17	1197.0
18	1538.5
19	1989.0
20	2674.5
21	3647.0
22	4924.5
23	7024.5
24	10014.5
25	13570.5
26	18745.5
27	24620.5
28	29440.5
29	37308.5
30	47333.5
31	56776.0
32	66995.5
33	76228.5
34	84492.5
35	92188.5
36	101311.0
37	112801.5
38	117623.5
39	119032.0
40	120485.0
41	120195.0
42	122013.0
43	128121.5
44	135407.5
45	145715.0
46	155139.5
47	160685.0
48	165501.0
49	163993.5
50	159067.5
51	156651.5
52	152714.5
53	147246.5
54	141085.5
55	127941.0
56	115170.0
57	99255.0
58	83661.0
59	75506.5
60	63281.0
61	49016.0
62	38677.5
63	29762.0
64	20916.5
65	14162.5
66	10417.0
67	8223.0
68	6399.0
69	4801.0
70	3664.5
71	2741.0
72	2153.0
73	1756.5
74	1411.5
75	1084.0
76	730.5
77	491.0
78	302.0
79	181.5
80	132.0
81	103.5
82	70.0
83	47.5
84	44.0
85	37.0
86	34.5
87	35.0
88	29.5
89	21.5
90	18.5
91	16.5
92	14.0
93	13.5
94	10.0
95	7.0
96	5.0
97	7.0
98	9.0
99	8.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09930297145817728
2	0.06528994660331626
3	0.08303696399991164
4	0.0844175664847953
5	0.08481919629857963
6	0.09380566338200418
7	0.08943793915709951
8	0.09064282859845252
9	0.07460273791044056
10-11	0.08281104722965796
12-13	0.07078725467948936
14-15	0.0810162639993092
16-17	0.08224625530402373
18-19	0.08261023232276579
20-21	0.07303387145034551
22-23	0.07767771617222691
24-25	0.07922148076896043
26-27	0.07142735219520815
28-29	0.08102881493098996
30-31	0.08498235841042952
32-33	0.08650102114380155
34-35	0.08873508698297691
36-37	0.08884804536810377
38-39	0.08967640685903396
40-41	0.08498235841042952
42-43	0.09106956027559839
44-45	0.08739213729313554
46-47	0.08320012611176153
48-49	0.09266352859905497
50-51	0.08857192487112703
52-53	0.09390607083545026
54-55	0.08558480313110603
56-57	0.09272628325745877
58-59	0.09774665592976299
60-61	0.09521136773024935
62-63	0.09695594723387506
64-65	0.09892644350775447
66-67	0.10079653232818778
68-69	0.09750818822782853
70-71	0.09976735593036543
72-73	0.09689319257547126
74-75	0.10052041183121105
76-77	0.09704380375564038
78-79	0.10483793232939267
80-81	0.09984266152044999
82-83	0.10436099692552378
84-85	0.10016898574414976
86-87	0.0963409515815178
88-89	0.09548748822722608
90-91	0.09622799319639094
92-93	0.09016589319458362
94-95	0.09017844412626438
96-97	0.08830835530583106
98-99	0.10249090810509046
100	0.24260950938910097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3983768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.279708538816436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.42409151558869	35.24911852929463
2	15.831094131612424	16.5528997410253
3	6.639502938099633	10.413338353393925
4	3.407898513331236	7.126557640272914
5	2.041903976825412	5.337507238634136
6	1.2484971485517073	3.9162640222696
7	0.821847890298682	3.0076177717639396
8	0.5871035389463746	2.4554881518579306
9	0.4058457007539435	1.9095745452433093
>10	1.5612500342389484	12.613154845694229
>50	0.024754854014584233	0.8351079832471573
>100	0.006124900083725769	0.480758784641884
>500	0.0	0.0
>1k	8.485765457337434E-5	0.10261239266111206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.510186336152105E-5	0.0
2	0.0	0.0	0.0	3.765279504228158E-4	0.0
3	0.0	0.0	0.0	3.765279504228158E-4	0.0
4	0.0	0.0	0.0	5.522409939534631E-4	0.0
5	0.0	0.0	0.0	5.522409939534631E-4	2.510186336152105E-5
6	0.0	0.0	0.0	5.522409939534631E-4	2.510186336152105E-5
7	0.0	0.0	0.0	6.526484473995473E-4	2.510186336152105E-5
8	0.0	0.0	0.0	7.781577642071527E-4	2.510186336152105E-5
9	0.0	0.0	0.0	9.036670810147579E-4	2.510186336152105E-5
10-11	0.0	0.0	0.0	0.0011797875779914894	2.510186336152105E-5
12-13	0.0	0.0	0.0	0.0013303987581606159	5.02037267230421E-5
14-15	0.0	0.0	0.0	0.0013931534165644184	1.1295838512684475E-4
16-17	0.0	0.0	0.0	0.0014810099383297422	1.7571304353064738E-4
18-19	0.0	0.0	0.0	0.0015814173917758263	1.7571304353064738E-4
20-21	0.0	0.0	0.0	0.0018324360253910367	2.63569565295971E-4
22-23	0.0	0.0	0.0	0.002008149068921684	3.2632422369977367E-4
24-25	0.0	0.0	0.0	0.0023846770193445	3.2632422369977367E-4
26-27	0.0	0.0	0.0	0.0032255894419554554	3.2632422369977367E-4
28-29	2.510186336152105E-5	0.0	0.0	0.008195758387536623	3.2632422369977367E-4
30-31	2.510186336152105E-5	0.0	0.0	0.023457691311341424	3.2632422369977367E-4
32-33	2.510186336152105E-5	0.0	0.0	0.057884896911667545	3.2632422369977367E-4
34-35	2.510186336152105E-5	0.0	0.0	0.11150247705187652	4.267316771458579E-4
36-37	2.510186336152105E-5	0.0	0.0	0.1991707348419888	4.267316771458579E-4
38-39	2.510186336152105E-5	0.0	0.0	0.32040018394645475	4.392826088266184E-4
40-41	2.510186336152105E-5	0.0	0.0	0.4505407945442606	4.5183354050737893E-4
42-43	2.510186336152105E-5	0.0	0.0	0.5844717865096563	4.643844721881395E-4
44-45	2.510186336152105E-5	0.0	0.0	0.7535955909079043	5.02037267230421E-4
46-47	2.510186336152105E-5	0.0	0.0	0.9643633866229158	5.271391305919421E-4
48-49	2.510186336152105E-5	0.0	0.0	1.1912089258209815	5.522409939534631E-4
50-51	2.510186336152105E-5	0.0	0.0	1.4249825793068271	5.773428573149842E-4
52-53	2.510186336152105E-5	0.0	0.0	1.6570869588791315	6.651993790803079E-4
54-55	2.510186336152105E-5	0.0	0.0	1.9143308546079993	6.777503107610684E-4
56-57	2.510186336152105E-5	0.0	0.0	2.230451170851315	6.777503107610684E-4
58-59	2.510186336152105E-5	0.0	0.0	2.5775220846193854	6.777503107610684E-4
60-61	2.510186336152105E-5	0.0	0.0	2.938800653050077	6.777503107610684E-4
62-63	2.510186336152105E-5	0.0	0.0	3.3030537922891092	7.279540374841105E-4
64-65	2.510186336152105E-5	0.0	0.0	3.6872001582421468	7.279540374841105E-4
66-67	2.510186336152105E-5	0.0	0.0	4.131490588809388	7.279540374841105E-4
68-69	2.510186336152105E-5	0.0	0.0	4.621290697651068	7.279540374841105E-4
70-71	2.510186336152105E-5	0.0	0.0	5.141752732588845	7.530559008456316E-4
72-73	2.510186336152105E-5	0.0	0.0	5.671414600448621	8.785652176532368E-4
74-75	2.510186336152105E-5	0.0	0.0	6.217078906201365	9.664217394185605E-4
76-77	2.510186336152105E-5	0.0	0.0	6.816272935572553	0.001004074534460842
78-79	2.510186336152105E-5	0.0	0.0	7.466712418996287	0.0010793801245454052
80-81	2.510186336152105E-5	0.0	0.0	8.15392613224465	0.0011044819879069264
82-83	2.510186336152105E-5	0.0	0.0	8.86029256723785	0.00119233850967225
84-85	2.510186336152105E-5	0.0	0.0	9.562818417136741	0.0012048894413530106
86-87	2.510186336152105E-5	0.0	0.0	10.297700568908631	0.0012425422363952921
88	2.510186336152105E-5	0.0	0.0	10.877591265354809	0.0013052968947990947
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3485	0.0	36.436226	1
GTATCAA	5505	0.0	33.745213	1
ATCAACG	5690	0.0	30.653961	3
CAACGCA	5770	0.0	30.39153	5
TATCAAC	6805	0.0	27.22065	2
TCAACGC	6925	0.0	25.798164	4
AACGCAG	7085	0.0	25.668447	6
GTACTAG	1815	0.0	25.652575	1
GTGATCG	1185	0.0	24.58648	8
CGCAGAG	7170	0.0	24.446276	8
ACGCAGA	7245	0.0	24.387793	7
GTACATG	11865	0.0	23.782412	1
TACATGG	11665	0.0	23.29558	2
TGATCGC	1285	0.0	23.037962	9
ACATGGG	11540	0.0	22.773563	3
GTATAAG	2130	0.0	22.300478	1
CATGGGG	9020	0.0	21.317768	4
CTAACGC	765	0.0	20.895004	3
GCATCGT	1640	0.0	19.770983	8
GCAGAGT	8525	0.0	19.733099	9
>>END_MODULE