FastQCFastQC Report
Mon 16 Jan 2017
ERR1146588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983768
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136700.3431424721519928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124610.31279431934791385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74210.18628092800584772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.060.4973531
GTATCAA130800.042.674051
ATCAACG162550.033.6729743
CAACGCA166650.032.8869255
TATCAAC172700.032.2665332
TCAACGC175600.031.331184
AACGCAG178600.031.0274476
ACGCAGA194750.028.1389267
CGCAGAG197150.027.701028
GTGGTAT19950.024.9852581
GCAGAGT216050.024.9290569
GTACTAG18300.023.8975141
GTACATG119350.023.2067181
TACATGG116850.022.8584082
GAGTACT155200.022.78716512-13
ACATGGG114250.022.5136833
AGAGTAC203300.022.2157810-11
CAGAGTA213200.022.12104810-11
TAACGCC7800.021.1003134
AGTACTT163250.020.6990912-13
Produced by FastQC (version 0.11.5)