FastQCFastQC Report
Mon 16 Jan 2017
ERR1146587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4827355
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.035.673091
GTATCAA69650.031.4007531
CAACGCA63650.030.8023365
ATCAACG63650.030.7307013
GTACTAG21350.026.8763431
TATCAAC79300.026.4447362
AACGCAG76450.026.433636
TCAACGC76950.025.6022684
GTACATG141900.024.826051
TACATGG138350.024.6057682
ACGCAGA82750.023.5117347
ACATGGG140350.023.1830843
CGCAGAG82500.023.1830358
GTGATCG14000.022.8249958
TGATCGC15250.022.1862289
CATGGGG112450.021.6592464
TCTAACG7850.020.3651122
GTATAAG28900.019.0412851
ATGGGGG78100.018.6017365
GCATCGT22900.017.8530988
Produced by FastQC (version 0.11.5)