##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146587_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4827355
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295512345787703	31.0	31.0	34.0	28.0	34.0
2	31.47425329191659	33.0	31.0	34.0	28.0	34.0
3	31.49615990537261	33.0	31.0	34.0	28.0	34.0
4	34.99608398387937	37.0	35.0	37.0	32.0	37.0
5	35.057969426321456	37.0	35.0	37.0	32.0	37.0
6	35.06375064605773	37.0	35.0	37.0	32.0	37.0
7	35.022772097763685	37.0	35.0	37.0	32.0	37.0
8	35.01002971606604	37.0	35.0	37.0	32.0	37.0
9	36.59821620742622	39.0	37.0	39.0	32.0	39.0
10-11	36.6044695283442	39.0	37.0	39.0	32.0	39.0
12-13	36.561271752336424	39.0	37.0	39.0	32.0	39.0
14-15	37.90756283720589	40.0	38.0	41.0	32.0	41.0
16-17	37.91815911612053	40.0	38.0	41.0	32.0	41.0
18-19	37.92386513939828	40.0	38.0	41.0	32.0	41.0
20-21	38.0255836788469	40.0	38.0	41.0	32.0	41.0
22-23	38.02494792697036	40.0	38.0	41.0	32.0	41.0
24-25	38.03952464651968	40.0	38.0	41.0	32.0	41.0
26-27	38.101013080662185	40.0	38.0	41.0	32.0	41.0
28-29	38.07919523217166	40.0	38.0	41.0	32.0	41.0
30-31	38.04573539754172	40.0	38.0	41.0	32.0	41.0
32-33	38.02167936685825	40.0	38.0	41.0	32.0	41.0
34-35	37.977780171543216	40.0	38.0	41.0	32.0	41.0
36-37	37.90634105011958	40.0	38.0	41.0	32.0	41.0
38-39	37.85482588291103	40.0	38.0	41.0	31.5	41.0
40-41	37.808845630785385	40.0	38.0	41.0	31.0	41.0
42-43	37.65335458030329	40.0	37.5	41.0	31.0	41.0
44-45	37.52940958350898	40.0	37.0	41.0	31.0	41.0
46-47	37.425213496832114	40.0	37.0	41.0	30.5	41.0
48-49	37.33032830608066	40.0	37.0	41.0	30.0	41.0
50-51	37.20933896512686	40.0	36.0	41.0	30.0	41.0
52-53	37.09356780265797	40.0	36.0	41.0	30.0	41.0
54-55	36.99033414778901	39.5	36.0	41.0	30.0	41.0
56-57	36.818400966989174	39.0	35.0	41.0	30.0	41.0
58-59	36.642574246145145	39.0	35.0	41.0	30.0	41.0
60-61	36.44305287263936	39.0	35.0	41.0	30.0	41.0
62-63	36.21987806987471	38.5	35.0	40.0	29.5	41.0
64-65	35.93922468929672	38.0	35.0	40.0	29.0	41.0
66-67	35.70829305074932	37.0	35.0	40.0	29.0	41.0
68-69	35.45253756974575	37.0	34.5	40.0	29.0	41.0
70-71	35.16634492387654	36.0	34.0	39.0	29.0	41.0
72-73	34.819730887825735	36.0	34.0	39.0	28.0	41.0
74-75	34.47906472592133	35.0	34.0	38.5	28.0	40.0
76-77	34.1870436294824	35.0	34.0	37.5	28.0	39.5
78-79	33.862597012235476	35.0	34.0	37.0	27.5	39.0
80-81	33.602509966638046	35.0	34.0	36.5	27.0	39.0
82-83	33.31912248011592	35.0	33.5	36.0	27.0	37.5
84-85	33.104734476747616	35.0	33.0	36.0	27.0	37.0
86-87	32.88804397024872	35.0	33.0	35.5	26.5	37.0
88-89	32.6985435088159	35.0	33.0	35.0	26.0	36.0
90-91	32.544836250907586	35.0	33.0	35.0	26.0	36.0
92-93	32.39107855129776	35.0	33.0	35.0	26.0	36.0
94-95	32.26825362957562	35.0	33.0	35.0	25.0	36.0
96-97	32.17135449951371	35.0	33.0	35.0	25.0	35.0
98-99	32.063482590362625	35.0	33.0	35.0	25.0	35.0
100	30.925752301208426	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.376341348512117
1101	2	0.3803624292891854
1101	3	0.37618988973031264
1101	4	0.3757350050208146
1101	5	0.31921985563991484
1101	6	0.34091736917940096
1101	7	0.38580805791473693
1101	8	0.44098479698745763
1101	9	0.5413878478402268
1101	10-11	0.5604645982526932
1101	12-13	0.5559872636779488
1101	14-15	0.6257209051307058
1101	16-17	0.6387094070813788
1101	18-19	0.6010569787169047
1101	20-21	0.603969924271972
1101	22-23	0.606881648713248
1101	24-25	0.5973409896241861
1101	26-27	0.630443043319417
1101	28-29	0.6118371585309177
1101	30-31	0.6423937281716761
1101	32-33	0.6693443000699801
1101	34-35	0.5753563297985878
1101	36-37	0.591615665428904
1101	38-39	0.600817420676357
1101	40-41	0.5740424952597962
1101	42-43	0.5768368385860185
1101	44-45	0.5232253361613814
1101	46-47	0.4933958349949563
1101	48-49	0.5548814112436276
1101	50-51	0.5414216342820879
1101	52-53	0.4808455762534436
1101	54-55	0.4561453762458072
1101	56-57	0.47810649000459904
1101	58-59	0.415504416227094
1101	60-61	0.4318228855359436
1101	62-63	0.43005289650580636
1101	64-65	0.42162101194035273
1101	66-67	0.40998427538384163
1101	68-69	0.40816008821413874
1101	70-71	0.39854820479699526
1101	72-73	0.47960965622246476
1101	74-75	0.42022052327331494
1101	76-77	0.38750060025697053
1101	78-79	0.4004130623992097
1101	80-81	0.3922301606207199
1101	82-83	0.40279917362190787
1101	84-85	0.3378181092942967
1101	86-87	0.339916644931634
1101	88-89	0.3621313063889531
1101	90-91	0.29490434986661285
1101	92-93	0.3327875517579386
1101	94-95	0.3780491670340922
1101	96-97	0.3807454669157835
1101	98-99	0.3894056019125429
1101	100	0.35824244493369406
1102	1	0.20451986969884217
1102	2	0.24531322905622588
1102	3	0.20745888076818986
1102	4	0.25525973694399084
1102	5	0.2609890496591305
1102	6	0.252050087391936
1102	7	0.27725354831876103
1102	8	0.3086085806624652
1102	9	0.3379028013050629
1102	10-11	0.33490953048870153
1102	12-13	0.34254302720280805
1102	14-15	0.4125139501306734
1102	16-17	0.3890432879584296
1102	18-19	0.34490623245710594
1102	20-21	0.3869189260152268
1102	22-23	0.36196543741633036
1102	24-25	0.40897922917903173
1102	26-27	0.3639272547846275
1102	28-29	0.3996563147415131
1102	30-31	0.41805078676107854
1102	32-33	0.44124817768275193
1102	34-35	0.42366530823245085
1102	36-37	0.44507320810389217
1102	38-39	0.41275081553582993
1102	40-41	0.41423926922224297
1102	42-43	0.48127781711147577
1102	44-45	0.3678373157327144
1102	46-47	0.32712027740433314
1102	48-49	0.39277261001327446
1102	50-51	0.37480390886064185
1102	52-53	0.3411729790863376
1102	54-55	0.3381563904409717
1102	56-57	0.4221819947450527
1102	58-59	0.42747226078698475
1102	60-61	0.3529185081070736
1102	62-63	0.3094053186328338
1102	64-65	0.38268609586095437
1102	66-67	0.4031661228753549
1102	68-69	0.3729912616355122
1102	70-71	0.3634637188129517
1102	72-73	0.37394082084201585
1102	74-75	0.37935271200343834
1102	76-77	0.3019293807787662
1102	78-79	0.2836838284143326
1102	80-81	0.21911854040406098
1102	82-83	0.23308364758250377
1102	84-85	0.2131613742883829
1102	86-87	0.23398462521613084
1102	88-89	0.18935661949237925
1102	90-91	0.238881833183477
1102	92-93	0.25237632172932223
1102	94-95	0.2527128650425823
1102	96-97	0.21947786504595967
1102	98-99	0.2947433489722542
1102	100	0.24523793923356862
1103	1	0.22263521038836487
1103	2	0.2559892697719768
1103	3	0.1748524950013568
1103	4	0.19920984783847473
1103	5	0.27580160769757356
1103	6	0.31268667831569985
1103	7	0.2538193576712402
1103	8	0.28507413048232877
1103	9	0.4201609729901392
1103	10-11	0.38981414815225435
1103	12-13	0.39684260429762475
1103	14-15	0.4744995424258889
1103	16-17	0.4930069020181378
1103	18-19	0.48542833225521775
1103	20-21	0.457415844106869
1103	22-23	0.3793125047811614
1103	24-25	0.4568316159625354
1103	26-27	0.41537695881166314
1103	28-29	0.42835219959361837
1103	30-31	0.4462558003727679
1103	32-33	0.47055261017303707
1103	34-35	0.4054760821204084
1103	36-37	0.408327414051719
1103	38-39	0.47753606819506444
1103	40-41	0.38261763538096005
1103	42-43	0.4740374959359599
1103	44-45	0.42011741323683083
1103	46-47	0.4018733797153118
1103	48-49	0.34437422227945547
1103	50-51	0.38457276786592587
1103	52-53	0.35417273766726254
1103	54-55	0.28362500316467987
1103	56-57	0.32425190267689885
1103	58-59	0.332868446365552
1103	60-61	0.2707803232202508
1103	62-63	0.2522590556380777
1103	64-65	0.25053444667103264
1103	66-67	0.23831660790779807
1103	68-69	0.19167842525897782
1103	70-71	0.21678245863001422
1103	72-73	0.2935077707503524
1103	74-75	0.24252677904476627
1103	76-77	0.19266359903734553
1103	78-79	0.18150972023602208
1103	80-81	0.21250805809575724
1103	82-83	0.2564309434755714
1103	84-85	0.2400686774192664
1103	86-87	0.23290813261922239
1103	88-89	0.22638059761053597
1103	90-91	0.25634366228701566
1103	92-93	0.2262395075379331
1103	94-95	0.23525985100549462
1103	96-97	0.2982286473831053
1103	98-99	0.2813660235600963
1103	100	0.31336264474636977
1104	1	0.19299052519183135
1104	2	0.14576991501586534
1104	3	0.14051210091601618
1104	4	0.124073793549762
1104	5	0.15314479452768381
1104	6	0.23256427835467264
1104	7	0.1879763900856446
1104	8	0.238586292418141
1104	9	0.24928530365424706
1104	10-11	0.3018622475174979
1104	12-13	0.29539840887164104
1104	14-15	0.3832197217049611
1104	16-17	0.3496602439055039
1104	18-19	0.38494713233872346
1104	20-21	0.4069126575043569
1104	22-23	0.3608696049070872
1104	24-25	0.39039715051195145
1104	26-27	0.3964464043773219
1104	28-29	0.3949554637585351
1104	30-31	0.38483346360680315
1104	32-33	0.38916857629451584
1104	34-35	0.3532618814773585
1104	36-37	0.39275865324729864
1104	38-39	0.364976905072524
1104	40-41	0.38767835170772713
1104	42-43	0.38617828078507443
1104	44-45	0.34256833571744494
1104	46-47	0.36873736347877184
1104	48-49	0.3531606283373563
1104	50-51	0.3192257231859301
1104	52-53	0.32265673921922655
1104	54-55	0.3616558673863537
1104	56-57	0.3532187613706981
1104	58-59	0.35328632498197976
1104	60-61	0.2742720543414876
1104	62-63	0.31382333233212023
1104	64-65	0.37242888283922326
1104	66-67	0.2964395192615328
1104	68-69	0.2532238092284871
1104	70-71	0.29674629306537526
1104	72-73	0.30458588652069807
1104	74-75	0.2809152838754372
1104	76-77	0.24225486716680678
1104	78-79	0.261839495632195
1104	80-81	0.26142256660575214
1104	82-83	0.24078092172893406
1104	84-85	0.22240086823916982
1104	86-87	0.15276972940918654
1104	88-89	0.18611011069568661
1104	90-91	0.1261927054172034
1104	92-93	0.10845581705357432
1104	94-95	0.2030333083011726
1104	96-97	0.15607898784333685
1104	98-99	0.13386589997944753
1104	100	0.10107625700329237
1105	1	0.1869745693538647
1105	2	0.1347401388745908
1105	3	0.09881401863862749
1105	4	0.20062002950423619
1105	5	0.15225156074260582
1105	6	0.19387950791588793
1105	7	0.24485044638806386
1105	8	0.22855644983268775
1105	9	0.21288357908152733
1105	10-11	0.27612092248036646
1105	12-13	0.304803947699277
1105	14-15	0.3490222420856597
1105	16-17	0.2902866316331867
1105	18-19	0.2997264572881164
1105	20-21	0.2955144411328732
1105	22-23	0.2938517803373273
1105	24-25	0.35512492350238745
1105	26-27	0.34913007998368784
1105	28-29	0.28506900206318164
1105	30-31	0.32900607411983884
1105	32-33	0.3601959328244817
1105	34-35	0.401389603005633
1105	36-37	0.36796041650644185
1105	38-39	0.31627584597628555
1105	40-41	0.31945201487781816
1105	42-43	0.33909188146343894
1105	44-45	0.3008912256851133
1105	46-47	0.2538901641350364
1105	48-49	0.2963340962966825
1105	50-51	0.2750046541735074
1105	52-53	0.24139271015549468
1105	54-55	0.2097905924900303
1105	56-57	0.22465089291956275
1105	58-59	0.20903427434107869
1105	60-61	0.2932803159950623
1105	62-63	0.2322553874316995
1105	64-65	0.2974301356155351
1105	66-67	0.19985482692244005
1105	68-69	0.18257100929908887
1105	70-71	0.1271414918279561
1105	72-73	0.1108969737962866
1105	74-75	0.2115949911311148
1105	76-77	0.1933901027020113
1105	78-79	0.16857142168299077
1105	80-81	0.1749598365299576
1105	82-83	0.12908539545727393
1105	84-85	0.13871922674817228
1105	86-87	0.1310899970979733
1105	88-89	0.12818762385689553
1105	90-91	0.11348321492260283
1105	92-93	0.1684556892105249
1105	94-95	0.13924036725529731
1105	96-97	0.1405494038086843
1105	98-99	0.17588132375092158
1105	100	0.35867537149140105
1106	1	0.11924604362626212
1106	2	0.14302838233915338
1106	3	0.15579443158106798
1106	4	0.08833513269961912
1106	5	0.10800937222007434
1106	6	0.15783465598919122
1106	7	0.192845281590742
1106	8	0.23559767878958837
1106	9	0.25291688478387186
1106	10-11	0.22561005867636652
1106	12-13	0.2197095181120332
1106	14-15	0.22927413270409147
1106	16-17	0.23679811922291094
1106	18-19	0.2761559275745995
1106	20-21	0.3128036713299167
1106	22-23	0.3170195103173157
1106	24-25	0.33088673265427815
1106	26-27	0.30900769297600306
1106	28-29	0.29864475783868727
1106	30-31	0.2574129853934366
1106	32-33	0.29990453577650555
1106	34-35	0.33279705978232244
1106	36-37	0.25705781155124185
1106	38-39	0.25707542417831775
1106	40-41	0.25860806001440295
1106	42-43	0.26482502308167
1106	44-45	0.20414719535477133
1106	46-47	0.23477503018355605
1106	48-49	0.22528870753058072
1106	50-51	0.22931306114048056
1106	52-53	0.18180533080125372
1106	54-55	0.19515495792574455
1106	56-57	0.1576440526263454
1106	58-59	0.1932445124100184
1106	60-61	0.17555347659296672
1106	62-63	0.10549476303951622
1106	64-65	0.1909090994010043
1106	66-67	0.1308602265676697
1106	68-69	0.15048920796926524
1106	70-71	0.18806165622767423
1106	72-73	0.19917336038129463
1106	74-75	0.2008454859670934
1106	76-77	0.13957465973523853
1106	78-79	0.20219811377140218
1106	80-81	0.15863936166069692
1106	82-83	0.21780304237412196
1106	84-85	0.24517383102017476
1106	86-87	0.1309308327233225
1106	88-89	0.11828276665152515
1106	90-91	0.09552353883324827
1106	92-93	0.14564214947095167
1106	94-95	0.13710083789791128
1106	96-97	0.1380028666394395
1106	98-99	0.1913371932311847
1106	100	0.26307835192476503
1107	1	0.05366980719580994
1107	2	0.09000899500242099
1107	3	0.05101358562051317
1107	4	0.0407832243447146
1107	5	0.0953446457149596
1107	6	0.161336213392822
1107	7	0.14497003240470718
1107	8	0.1611961259609842
1107	9	0.1940122047633679
1107	10-11	0.23736176112586804
1107	12-13	0.28114960870341577
1107	14-15	0.25601176184299845
1107	16-17	0.23808679095824914
1107	18-19	0.24055702290040415
1107	20-21	0.2718663792277809
1107	22-23	0.2712356900057742
1107	24-25	0.37028873861463296
1107	26-27	0.22771174782038628
1107	28-29	0.24678422217576212
1107	30-31	0.2760433735452281
1107	32-33	0.28968007674442475
1107	34-35	0.28014827885242966
1107	36-37	0.24472464344344047
1107	38-39	0.26985015533713863
1107	40-41	0.24610602383555147
1107	42-43	0.27857822742252836
1107	44-45	0.2450441572739308
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2216	60-61	-0.4183311775239176
2216	62-63	-0.3957879628101111
2216	64-65	-0.3771881431235826
2216	66-67	-0.33354878813547373
2216	68-69	-0.4117252133708078
2216	70-71	-0.36213009514091254
2216	72-73	-0.3303126980188722
2216	74-75	-0.27895722714008286
2216	76-77	-0.34241784575321077
2216	78-79	-0.3045786860170736
2216	80-81	-0.30881721046319655
2216	82-83	-0.28890260639725085
2216	84-85	-0.3179544342504883
2216	86-87	-0.3645577772780655
2216	88-89	-0.34116394704141584
2216	90-91	-0.34694112959787304
2216	92-93	-0.28600274781683055
2216	94-95	-0.3325957929644794
2216	96-97	-0.277126614361201
2216	98-99	-0.2859877386174432
2216	100	-0.33613374237865656
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1530.0
1	376.0
2	288.0
3	240.0
4	203.0
5	283.0
6	463.0
7	1147.0
8	3482.0
9	5187.0
10	5505.0
11	6261.0
12	7722.0
13	8792.0
14	10953.0
15	12261.0
16	13121.0
17	14559.0
18	15940.0
19	17723.0
20	19826.0
21	22315.0
22	24610.0
23	28329.0
24	34345.0
25	41755.0
26	45031.0
27	43736.0
28	48142.0
29	58544.0
30	68583.0
31	78402.0
32	99244.0
33	132211.0
34	192216.0
35	292209.0
36	528518.0
37	1098095.0
38	1400419.0
39	443883.0
40	906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98839509111774	20.173140496039142	13.180293303683072	24.658171109160048
2	14.75058119468545	20.69411695202125	40.89009072130329	23.665211131990013
3	17.70833462912076	26.510628981436447	30.448392207694198	25.3326441817486
4	12.096763325358669	17.814160791061354	40.267490961896385	29.82158492168359
5	12.978933558024355	38.47644393284481	35.840070193829185	12.704552315301656
6	29.300785038140525	39.17417247118544	18.833907029140402	12.691135461533628
7	25.82627406812723	32.98759935774095	23.69694562029751	17.489180953834314
8	24.822259170999647	36.082367989503396	21.31365966443792	17.781713175059036
9	25.760509520336573	15.902733061977239	21.097404177811352	37.23935323987483
10-11	23.92599128003016	26.847188432226798	29.470798653991103	19.75602163375194
12-13	24.497199008173386	24.65122609081959	28.72723254793341	22.124342353073605
14-15	22.120855491576354	25.976314621636327	26.14190473830925	25.760925148478066
16-17	21.93728307083276	28.53633546058075	26.987361948511918	22.53901952007457
18-19	21.750672698919796	28.598678766142104	28.047644613033683	21.603003921904413
20-21	22.999012590047	27.764048202522073	27.352582471949617	21.88435673548131
22-23	22.882064501469674	27.607909828469595	26.98891141279042	22.521114257270316
24-25	22.589591763770127	27.68353909844684	27.379408450911548	22.347460686871486
26-27	22.46475928597505	27.906600939144273	27.09509587244621	22.533543902434474
28-29	22.278985997769578	28.133490680556456	27.143391157470305	22.44413216420366
30-31	22.744656822263963	27.86644227493737	27.058437154592895	22.330463748205776
32-33	22.629702478432378	28.125035960289267	26.7090791605316	22.536182400746753
34-35	22.69218404367737	27.97536424848942	27.2060879433877	22.126363764445507
36-37	22.726440997591187	27.780508374858165	27.11527825221974	22.377772375330906
38-39	22.558639674018067	27.890690638137993	27.292723961214588	22.257945726629348
40-41	22.70133796638357	27.48399682896338	27.47950794816991	22.33515725648314
42-43	22.4641668881851	27.63587926782387	27.220910621148743	22.679043222842292
44-45	22.498634394029132	28.007803521931233	27.21450282353095	22.27905926050869
46-47	22.522433144264415	27.693599709233197	27.0856515994144	22.698315547087986
48-49	22.623410567481	27.460946186994867	27.29814226448095	22.617500981043186
50-51	22.488219630948812	27.59055115498002	27.15356923514583	22.76765997892534
52-53	22.280897488972986	27.726976912917202	27.173239018838117	22.81888657927169
54-55	22.301595768006703	27.574969020680285	27.20563270537037	22.91780250594265
56-57	22.574420188499452	27.569654671331723	27.06973413981664	22.78619100035219
58-59	22.368761565712322	27.628127978261645	27.33676119411651	22.66634926190952
60-61	22.50005132161984	27.729517023122103	27.08275056510806	22.68768109015
62-63	22.49091570577452	27.449410535655833	27.09901966242828	22.96065409614137
64-65	22.06977131915243	27.595257419923964	27.206360213290026	23.128611047633584
66-67	22.5016931803713	27.44987543252308	27.030914383759068	23.017517003346548
68-69	22.232686049481035	27.512395971867733	27.209302108572363	23.045615870078873
70-71	22.31397358021248	27.30520966772822	27.168501964557606	23.212314787501693
72-73	22.500041731679094	27.4089106310649	27.247510122135406	22.843537515120605
74-75	22.442099784231814	27.67573805472694	27.106684458987974	22.77547770205327
76-77	22.39348164473052	27.537927608690495	27.030581073681837	23.038009672897154
78-79	22.34345656011308	27.515080529029607	27.077678826207276	23.063784084650045
80-81	22.249638660149763	27.61071929959169	27.310625776336334	22.82901626392221
82-83	22.62264797924806	27.172425892559126	27.567502439547386	22.637423688645423
84-85	22.78893807791737	27.39351016568455	26.973256580615185	22.844295175782893
86-87	22.27044439882467	27.54430820720502	27.29613662708114	22.889110766889168
88-89	22.66609725582752	27.138045944155643	27.138232568247133	23.0576242317697
90-91	22.63578919865775	27.09612903259252	27.241748449067675	23.026333319682053
92-93	22.750833671570984	27.194203358324042	27.14859700059665	22.90636596950833
94-95	22.265621355207188	27.207651978464813	27.33902780643215	23.18769885989585
96-97	22.44144704069303	27.119038374912073	27.215650822563912	23.22386376183098
98-99	22.416176469978314	27.356199870018212	27.036709693928973	23.190913966074504
100	22.87955736305068	27.115105708561348	26.67216141861786	23.33317550977011
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1999.0
1	1315.5
2	657.0
3	657.0
4	588.0
5	541.0
6	532.0
7	513.5
8	501.5
9	498.5
10	503.5
11	476.5
12	473.5
13	558.0
14	624.5
15	751.5
16	953.0
17	1225.0
18	1564.0
19	1912.0
20	2557.0
21	3537.5
22	5466.5
23	8378.5
24	11331.0
25	15184.5
26	21567.5
27	29555.0
28	37679.5
29	47570.5
30	57417.0
31	66486.0
32	79591.0
33	92212.0
34	103090.0
35	114171.5
36	126070.5
37	140307.0
38	148124.0
39	148751.5
40	148601.5
41	150821.5
42	155888.0
43	164688.5
44	175354.5
45	187839.5
46	197547.5
47	203727.5
48	207053.0
49	202337.5
50	194645.5
51	191179.0
52	185715.0
53	176361.0
54	165045.5
55	147137.0
56	130935.0
57	112680.5
58	94220.0
59	82916.0
60	68882.0
61	53160.0
62	40928.5
63	31195.5
64	22688.0
65	15401.5
66	10949.0
67	8203.5
68	6107.5
69	4687.5
70	3587.0
71	2598.5
72	2087.0
73	1770.5
74	1346.5
75	1042.5
76	846.0
77	588.0
78	394.0
79	294.0
80	215.5
81	169.0
82	126.0
83	84.0
84	58.0
85	46.5
86	38.5
87	30.0
88	25.5
89	30.0
90	25.0
91	20.5
92	19.5
93	16.0
94	19.5
95	20.0
96	14.5
97	12.0
98	10.5
99	11.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10177415996958997
2	0.06711750016313281
3	0.08354471548083785
4	0.08625841687632255
5	0.08574053493061935
6	0.09388163911707342
7	0.09071220160936994
8	0.09243156966910451
9	0.076252937685337
10-11	0.08407295506545509
12-13	0.0730835001776335
14-15	0.08285075367359558
16-17	0.0841868890935098
18-19	0.08411438562111136
20-21	0.07507216684913373
22-23	0.07948452102652488
24-25	0.0815042606147673
26-27	0.07461643073691493
28-29	0.08393830575957227
30-31	0.08787420854691648
32-33	0.08962464952339325
34-35	0.0920897675849404
36-37	0.0919240453623154
38-39	0.09344661828268276
40-41	0.08962464952339325
42-43	0.09489668773065167
44-45	0.09124044119398719
46-47	0.08795706965822898
48-49	0.09699928843020661
50-51	0.09323946550440149
52-53	0.09873937176776931
54-55	0.090722559248284
56-57	0.09712358009717537
58-59	0.10173272941393371
60-61	0.09997193079854289
62-63	0.1011630592736602
64-65	0.10359710441846519
66-67	0.10500574331077785
68-69	0.10195023983112905
70-71	0.10404248289176993
72-73	0.10108019816234771
74-75	0.10461215303204342
76-77	0.10174308705284778
78-79	0.10861020165287202
80-81	0.10409427108634024
82-83	0.10878628151441111
84-85	0.10416677455873868
86-87	0.1011941321904024
88-89	0.09998228843745695
90-91	0.10052088566098827
92-93	0.09451345509083131
94-95	0.09425451411797972
96-97	0.09275265647544048
98-99	0.10649724331440302
100	0.24549675754113792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4827355.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.252613804509245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20515464490035	37.00307914426608
2	15.69035907629399	17.024859828405088
3	6.63759951064217	10.803213685196074
4	3.3285150300096147	7.223225618624644
5	1.8984252412633214	5.149726572549563
6	1.2162333777674177	3.9590303844101697
7	0.7663144920480365	2.910219493291655
8	0.5054065826115788	2.1935702512546222
9	0.3924146790529543	1.9160569830492333
>10	1.3402392040981341	11.011398550865477
>50	0.01688079465671475	0.5893612224608769
>100	0.002457366655676105	0.21625826562651518
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0357638914063706E-4
2	0.0	0.0	0.0	8.286111131250964E-5	1.0357638914063706E-4
3	0.0	0.0	0.0	8.286111131250964E-5	1.0357638914063706E-4
4	0.0	0.0	0.0	1.0357638914063706E-4	1.0357638914063706E-4
5	0.0	0.0	0.0	1.657222226250193E-4	1.0357638914063706E-4
6	0.0	0.0	0.0	1.657222226250193E-4	1.4500694479689189E-4
7	0.0	0.0	0.0	1.657222226250193E-4	1.4500694479689189E-4
8	0.0	0.0	0.0	1.657222226250193E-4	1.4500694479689189E-4
9	0.0	0.0	0.0	1.8643750045314671E-4	1.4500694479689189E-4
10-11	0.0	0.0	0.0	3.314444452500386E-4	1.4500694479689189E-4
12-13	0.0	0.0	0.0	3.314444452500386E-4	5.696701402735037E-4
14-15	0.0	0.0	0.0	3.314444452500386E-4	8.182534742110328E-4
16-17	0.0	0.0	0.0	3.314444452500386E-4	0.0010875520859766892
18-19	0.0	0.0	0.0	3.314444452500386E-4	0.0012532743086017084
20-21	0.0	0.0	0.0	3.7287500090629343E-4	0.0014707847257970463
22-23	0.0	0.0	0.0	4.3502083439067563E-4	0.0015743611149376833
24-25	0.0	0.0	0.0	6.525312515860134E-4	0.001584718753851747
26-27	0.0	0.0	0.0	0.001450069447968919	0.0016675798651642566
28-29	0.0	0.0	0.0	0.004650579872414604	0.0017090104208205114
30-31	0.0	0.0	0.0	0.014769993091454844	0.0017504409764767662
32-33	0.0	1.0357638914063705E-5	0.0	0.04003227440285623	0.0018229444488752121
34-35	0.0	2.071527782812741E-5	0.0	0.08132818075322822	0.0018850902823595943
36-37	0.0	2.071527782812741E-5	0.0	0.1483421045272204	0.0018850902823595943
38-39	0.0	2.071527782812741E-5	0.0	0.24705040337824752	0.0019265208380158493
40-41	0.0	2.071527782812741E-5	0.0	0.35148647654875187	0.0019472361158439767
42-43	0.0	2.071527782812741E-5	0.0	0.4597445184785457	0.0019575937547580404
44-45	0.0	2.071527782812741E-5	0.0	0.5982572236763196	0.0020611701438986775
46-47	0.0	2.071527782812741E-5	0.0	0.761555758795448	0.0021958194497815058
48-49	0.0	2.071527782812741E-5	0.0	0.9389613981155311	0.002289038200008079
50-51	0.0	2.071527782812741E-5	0.0	1.1219394471713806	0.0023822569502346524
52-53	0.0	2.071527782812741E-5	0.0	1.3038092288634253	0.002672270839828436
54-55	0.0	2.071527782812741E-5	0.0	1.5038980974053078	0.0026929861176565634
56-57	0.0	2.071527782812741E-5	0.0	1.7446096257681485	0.0027654895900550095
58-59	0.0	2.071527782812741E-5	0.0	2.01866239379536	0.002817277784625328
60-61	0.0	2.071527782812741E-5	0.0	2.305009679213565	0.002837993062453455
62-63	0.0	2.071527782812741E-5	0.0	2.592071641716841	0.0032315833411878762
64-65	0.0	2.071527782812741E-5	0.0	2.889170156327844	0.003273013896844131
66-67	0.0	2.071527782812741E-5	0.0	3.2285071224303996	0.003283371535758195
68-69	0.0	2.071527782812741E-5	0.0	3.6116465435005294	0.0032937291746722584
70-71	0.0	2.071527782812741E-5	0.0	4.022130131303789	0.0033248020914144498
72-73	0.0	2.071527782812741E-5	0.0	4.433007309385782	0.003480166675125405
74-75	0.0	2.071527782812741E-5	0.0	4.850275150677752	0.003676961814492615
76-77	0.0	2.071527782812741E-5	0.0	5.308683533736383	0.0037494652868910613
78-79	0.0	2.071527782812741E-5	0.0	5.814892420383419	0.0037701805647191887
80-81	0.0	2.071527782812741E-5	0.0	6.354597911278537	0.0038426840371176348
82-83	0.0	2.071527782812741E-5	0.0	6.912574277218063	0.0040187638986567175
84-85	0.0	2.071527782812741E-5	0.0	7.467526212594682	0.004070552093227036
86-87	0.0	2.071527782812741E-5	0.0	8.047460773032023	0.004246631954766119
88	0.0	2.071527782812741E-5	0.0	8.502295770665302	0.0044123541773911385
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4285	0.0	35.67309	1
GTATCAA	6965	0.0	31.400753	1
CAACGCA	6365	0.0	30.802336	5
ATCAACG	6365	0.0	30.730701	3
GTACTAG	2135	0.0	26.876343	1
TATCAAC	7930	0.0	26.444736	2
AACGCAG	7645	0.0	26.43363	6
TCAACGC	7695	0.0	25.602268	4
GTACATG	14190	0.0	24.82605	1
TACATGG	13835	0.0	24.605768	2
ACGCAGA	8275	0.0	23.511734	7
ACATGGG	14035	0.0	23.183084	3
CGCAGAG	8250	0.0	23.183035	8
GTGATCG	1400	0.0	22.824995	8
TGATCGC	1525	0.0	22.186228	9
CATGGGG	11245	0.0	21.659246	4
TCTAACG	785	0.0	20.365112	2
GTATAAG	2890	0.0	19.041285	1
ATGGGGG	7810	0.0	18.601736	5
GCATCGT	2290	0.0	17.853098	8
>>END_MODULE