FastQCFastQC Report
Mon 16 Jan 2017
ERR1146587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4827355
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125530.2600388825764834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118440.24535175059634107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66330.13740443783396913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79900.059.7951431
GTATCAA149750.041.5443531
ATCAACG178750.033.6187323
CAACGCA181300.033.1328355
TATCAAC190300.032.2711452
AACGCAG193650.031.4065196
TCAACGC195250.030.8737224
CGCAGAG211800.028.425218
ACGCAGA211650.028.4231537
GCAGAGT241850.024.7959429
GTGGTAT25550.024.1101231
GTACATG135450.022.3576351
GAGTACT173850.022.35665512-13
TACATGG131100.022.1305662
CAGAGTA239300.022.08465210-11
GTGATCG14050.021.7429058
AGAGTAC228400.021.54390110-11
ACATGGG131450.021.248213
AGTACTT181850.020.41690312-13
TGATCGC15050.020.2979839
Produced by FastQC (version 0.11.5)