##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4827355
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.381911626553254	33.0	31.0	34.0	30.0	34.0
2	32.47116091524241	34.0	31.0	34.0	30.0	34.0
3	32.55298709127462	34.0	31.0	34.0	31.0	34.0
4	36.06247997091575	37.0	35.0	37.0	35.0	37.0
5	36.09900059142118	37.0	35.0	37.0	35.0	37.0
6	36.153322057317105	37.0	36.0	37.0	35.0	37.0
7	36.126655487321734	37.0	36.0	37.0	35.0	37.0
8	36.129929536982466	37.0	36.0	37.0	35.0	37.0
9	37.92607276655643	39.0	38.0	39.0	35.0	39.0
10-11	37.86836984228423	39.0	38.0	39.0	35.0	39.0
12-13	37.83803770387718	39.0	38.0	39.0	35.0	39.0
14-15	39.209895688218495	40.0	39.0	41.0	36.0	41.0
16-17	39.15646787940808	40.0	39.0	41.0	36.0	41.0
18-19	39.253273065685036	40.5	39.0	41.0	36.0	41.0
20-21	39.3121323167656	41.0	39.0	41.0	36.0	41.0
22-23	39.27637070403979	41.0	39.0	41.0	36.0	41.0
24-25	39.26654637995341	41.0	39.0	41.0	36.0	41.0
26-27	39.19098388247808	41.0	39.0	41.0	36.0	41.0
28-29	39.13291046546193	40.5	39.0	41.0	35.0	41.0
30-31	38.99662527408902	40.0	39.0	41.0	35.0	41.0
32-33	38.89670471717949	40.0	38.0	41.0	35.0	41.0
34-35	38.8294571250716	40.0	38.0	41.0	35.0	41.0
36-37	38.76351645984188	40.0	38.0	41.0	35.0	41.0
38-39	38.677577679702445	40.0	38.0	41.0	35.0	41.0
40-41	38.58709282826724	40.0	38.0	41.0	35.0	41.0
42-43	38.488906865146646	40.0	38.0	41.0	34.5	41.0
44-45	38.35120143432584	40.0	38.0	41.0	34.0	41.0
46-47	38.12324834200095	40.0	38.0	41.0	33.5	41.0
48-49	37.85354733596348	40.0	38.0	41.0	33.0	41.0
50-51	37.7510501092213	40.0	37.0	41.0	33.0	41.0
52-53	37.65615093565731	40.0	37.0	41.0	33.0	41.0
54-55	37.50510144789435	40.0	37.0	41.0	33.0	41.0
56-57	37.36055976823747	40.0	36.0	41.0	32.5	41.0
58-59	37.188058056637644	39.5	36.0	41.0	32.0	41.0
60-61	36.99466333012592	39.0	35.0	41.0	32.0	41.0
62-63	36.761269577232255	39.0	35.0	41.0	31.5	41.0
64-65	36.49729137384759	38.5	35.0	41.0	31.0	41.0
66-67	36.23543203265557	37.5	35.0	40.0	31.0	41.0
68-69	35.949955721093644	37.0	35.0	40.0	31.0	41.0
70-71	35.64718515626052	37.0	35.0	39.5	31.0	41.0
72-73	35.32227627758887	36.0	35.0	39.0	30.5	41.0
74-75	34.99810693433568	36.0	35.0	39.0	30.0	40.5
76-77	34.68943210101598	35.0	35.0	37.5	30.0	39.5
78-79	34.394932110855734	35.0	34.0	37.0	30.0	39.0
80-81	34.12117743567647	35.0	34.0	37.0	30.0	39.0
82-83	33.85698928295102	35.0	34.0	36.0	30.0	38.0
84-85	33.63604665494873	35.0	34.0	36.0	29.0	37.0
86-87	33.44084483117567	35.0	34.0	35.5	29.0	37.0
88-89	33.240612716487604	35.0	34.0	35.0	29.0	36.5
90-91	33.149395062099224	35.0	34.0	35.0	29.0	36.0
92-93	33.04915548991114	35.0	34.0	35.0	29.0	36.0
94-95	32.97435956129185	35.0	34.0	35.0	29.0	36.0
96-97	32.918565239142346	35.0	34.0	35.0	29.0	35.5
98-99	32.849683211613815	35.0	34.0	35.0	29.0	35.0
100	32.123826194675964	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33559418416369624
1101	2	0.3244007816644867
1101	3	0.29636440172839684
1101	4	0.2269555497718585
1101	5	0.22882166139189763
1101	6	0.23585236078390892
1101	7	0.21977674189912477
1101	8	0.23130627795338654
1101	9	0.2628551702441442
1101	10-11	0.28456878005984976
1101	12-13	0.3165065758736745
1101	14-15	0.4965329211713083
1101	16-17	0.49013195780461416
1101	18-19	0.43384726185717426
1101	20-21	0.39388562834867713
1101	22-23	0.41506195759424
1101	24-25	0.40192122158388344
1101	26-27	0.4065050665754697
1101	28-29	0.4243473257877426
1101	30-31	0.4924622215485357
1101	32-33	0.49190774581006735
1101	34-35	0.46086495322425236
1101	36-37	0.4724227315567546
1101	38-39	0.5144086340244769
1101	40-41	0.465405175557315
1101	42-43	0.5214453782624133
1101	44-45	0.5176896809435547
1101	46-47	0.5168921375557858
1101	48-49	0.5842428893489284
1101	50-51	0.5446515073092826
1101	52-53	0.4917524460240301
1101	54-55	0.5022339502581801
1101	56-57	0.47561901224017333
1101	58-59	0.456516194190165
1101	60-61	0.46466788456614694
1101	62-63	0.44288402397272364
1101	64-65	0.39758001370658036
1101	66-67	0.4249159338111639
1101	68-69	0.3892377230239674
1101	70-71	0.3392928507832309
1101	72-73	0.3693089843767581
1101	74-75	0.3983686609270194
1101	76-77	0.38904111781336326
1101	78-79	0.3343090530453665
1101	80-81	0.2786686337102253
1101	82-83	0.29978209895975994
1101	84-85	0.36183372142524206
1101	86-87	0.3129989048815389
1101	88-89	0.37003660105015257
1101	90-91	0.3390689782526408
1101	92-93	0.370832075092018
1101	94-95	0.40654169004967144
1101	96-97	0.34211474668421005
1101	98-99	0.34928180438771506
1101	100	0.41925702531521125
1102	1	0.25439191781423176
1102	2	0.2523273304213731
1102	3	0.21800806654102445
1102	4	0.14026896270669198
1102	5	0.15398889971944385
1102	6	0.1855931139777809
1102	7	0.18026785620068608
1102	8	0.19262093893288323
1102	9	0.20473246242659116
1102	10-11	0.21199729025042302
1102	12-13	0.20027245060013854
1102	14-15	0.3679278694933288
1102	16-17	0.45736713238302684
1102	18-19	0.3410592702318027
1102	20-21	0.29053995954958367
1102	22-23	0.29414244259777433
1102	24-25	0.3024669150378898
1102	26-27	0.2615936680863129
1102	28-29	0.31250504083627817
1102	30-31	0.317140166742945
1102	32-33	0.28882676182404055
1102	34-35	0.3278422441759119
1102	36-37	0.3092244655993781
1102	38-39	0.23948716517026014
1102	40-41	0.21552701584358402
1102	42-43	0.20670235056942232
1102	44-45	0.1921836655712994
1102	46-47	0.24295571290755902
1102	48-49	0.34007282262031424
1102	50-51	0.3379845892553419
1102	52-53	0.26909510288319183
1102	54-55	0.24624493444377293
1102	56-57	0.2072405959986412
1102	58-59	0.18147317926104733
1102	60-61	0.15167607653868487
1102	62-63	0.21912186807806222
1102	64-65	0.21967797030558955
1102	66-67	0.11873131891840671
1102	68-69	0.15204524685226772
1102	70-71	0.1557789460348502
1102	72-73	0.1238301428575852
1102	74-75	0.11322498942107728
1102	76-77	0.10990033909175168
1102	78-79	0.10712922747251241
1102	80-81	0.07817208826665478
1102	82-83	0.06098536170507174
1102	84-85	0.07131652430445001
1102	86-87	0.10160968583572583
1102	88-89	0.1242943422786027
1102	90-91	0.08460287407562106
1102	92-93	0.0621422135523062
1102	94-95	0.10521727547268966
1102	96-97	0.11566260391578709
1102	98-99	0.14356934481542538
1102	100	0.11663198805456432
1103	1	0.22485300929576368
1103	2	0.23104373162973957
1103	3	0.2697896625590417
1103	4	0.14839697529119888
1103	5	0.1894322914006139
1103	6	0.17639651155083413
1103	7	0.17146105980341275
1103	8	0.18021657647971523
1103	9	0.2551370834550326
1103	10-11	0.2236168511293144
1103	12-13	0.2498213940765055
1103	14-15	0.41163434332551674
1103	16-17	0.45122835091525815
1103	18-19	0.35021958159890687
1103	20-21	0.35537927558004156
1103	22-23	0.37153678288910186
1103	24-25	0.3501326807036591
1103	26-27	0.35564466883805324
1103	28-29	0.34840362880481024
1103	30-31	0.38343627913749856
1103	32-33	0.40585939908623914
1103	34-35	0.3922308780861101
1103	36-37	0.3648797738475551
1103	38-39	0.3729916782473168
1103	40-41	0.37032015135229557
1103	42-43	0.44808754252792227
1103	44-45	0.4087957483354856
1103	46-47	0.4178987988575855
1103	48-49	0.5136528552246915
1103	50-51	0.48254055350560066
1103	52-53	0.49347344875777566
1103	54-55	0.4832210529122065
1103	56-57	0.42112569530415556
1103	58-59	0.4708692514759605
1103	60-61	0.49396195502024653
1103	62-63	0.46632571478349405
1103	64-65	0.43163682356592403
1103	66-67	0.3432963569999714
1103	68-69	0.3674320116840164
1103	70-71	0.3199987643136808
1103	72-73	0.39356296530252166
1103	74-75	0.41971425745681756
1103	76-77	0.330935772436149
1103	78-79	0.3320702091808698
1103	80-81	0.31944612034155284
1103	82-83	0.32619096033386796
1103	84-85	0.2491938899531192
1103	86-87	0.2664681225035963
1103	88-89	0.37051744175264645
1103	90-91	0.3509212682093832
1103	92-93	0.316617992451107
1103	94-95	0.33107407623484164
1103	96-97	0.31888855804957217
1103	98-99	0.3203900517666938
1103	100	0.31626978360928604
1104	1	0.2408820413232391
1104	2	0.21472237358690904
1104	3	0.1983891222182308
1104	4	0.1479647097539072
1104	5	0.13886575860409067
1104	6	0.15398556162998034
1104	7	0.15164094699117214
1104	8	0.15456923616635976
1104	9	0.17215099098968523
1104	10-11	0.17747535004588855
1104	12-13	0.1588091387884134
1104	14-15	0.32353130089450133
1104	16-17	0.3643874758433725
1104	18-19	0.2938940129234524
1104	20-21	0.2685710293547672
1104	22-23	0.2411831626518861
1104	24-25	0.259294594109015
1104	26-27	0.2538832979072865
1104	28-29	0.2105429289080547
1104	30-31	0.2230756334739752
1104	32-33	0.2693160333042428
1104	34-35	0.2407160283157168
1104	36-37	0.24039125546747186
1104	38-39	0.2222767686603362
1104	40-41	0.2515517720653335
1104	42-43	0.2666579082804006
1104	44-45	0.2018600024471624
1104	46-47	0.18179108596725513
1104	48-49	0.31919737644125945
1104	50-51	0.22975394331736254
1104	52-53	0.2246478045114273
1104	54-55	0.2457441731616825
1104	56-57	0.21471316675169305
1104	58-59	0.21414455287937528
1104	60-61	0.1908052519171406
1104	62-63	0.20161161638342406
1104	64-65	0.20508829584705524
1104	66-67	0.19608784817916103
1104	68-69	0.16941963299309037
1104	70-71	0.2133859639904827
1104	72-73	0.21216200354282932
1104	74-75	0.13825929362238298
1104	76-77	0.13390772692007147
1104	78-79	0.13005119483163696
1104	80-81	0.12941767367736645
1104	82-83	0.1378843743567657
1104	84-85	0.15710283786752655
1104	86-87	0.15436900833677214
1104	88-89	0.14063232552104665
1104	90-91	0.11607527814348373
1104	92-93	0.14715541000325771
1104	94-95	0.10783746535313554
1104	96-97	0.09039052342635756
1104	98-99	0.13541876515170514
1104	100	0.17993499350612296
1105	1	0.1481821099822227
1105	2	0.13222316266496392
1105	3	0.15311538973924854
1105	4	0.09126294226183518
1105	5	0.08423021688693666
1105	6	0.13688753799791442
1105	7	0.09691206702535737
1105	8	0.05227727121707204
1105	9	0.11648302049500359
1105	10-11	0.12671478869264519
1105	12-13	0.15161441878306192
1105	14-15	0.2674576745893802
1105	16-17	0.3158582566103405
1105	18-19	0.2423353090654885
1105	20-21	0.19958875105149332
1105	22-23	0.19999644185844545
1105	24-25	0.18051910043177344
1105	26-27	0.19937936183804084
1105	28-29	0.20670341536835934
1105	30-31	0.27231862521668404
1105	32-33	0.2272167633094
1105	34-35	0.20270198490706548
1105	36-37	0.20649031612467894
1105	38-39	0.19781627913008037
1105	40-41	0.25591047159630165
1105	42-43	0.2659612492082104
1105	44-45	0.22458328032227115
1105	46-47	0.2584495373287865
1105	48-49	0.40139549347998127
1105	50-51	0.33232852049253125
1105	52-53	0.29042430092280114
1105	54-55	0.23540989096787968
1105	56-57	0.18222148317681786
1105	58-59	0.20555695298757115
1105	60-61	0.23345079438082905
1105	62-63	0.24639368760661995
1105	64-65	0.22631656905011255
1105	66-67	0.2205433030878936
1105	68-69	0.19390185370286162
1105	70-71	0.21567758260911774
1105	72-73	0.21056024565395148
1105	74-75	0.15132860456206743
1105	76-77	0.1355009334348125
1105	78-79	0.19808590605644127
1105	80-81	0.15862662368235192
1105	82-83	0.1430787462356804
1105	84-85	0.15700933928059868
1105	86-87	0.14619959201851884
1105	88-89	0.19956663232570548
1105	90-91	0.14589079849417175
1105	92-93	0.12970838341438196
1105	94-95	0.17310188410297656
1105	96-97	0.13008636558578246
1105	98-99	0.13151744655547049
1105	100	0.15159315283823105
1106	1	0.12259080535726952
1106	2	0.058750486243596356
1106	3	0.12890798593483765
1106	4	0.12978270120083124
1106	5	0.07169688875182345
1106	6	0.11971288511022493
1106	7	0.08914376876914787
1106	8	0.11830975572531344
1106	9	0.14673000684566517
1106	10-11	0.12026364989691274
1106	12-13	0.1440280270895613
1106	14-15	0.21665772199214217
1106	16-17	0.29411199073838645
1106	18-19	0.1855806780907514
1106	20-21	0.16686023615350365
1106	22-23	0.14266815079309225
1106	24-25	0.1852349948077716
1106	26-27	0.1597804473984823
1106	28-29	0.15983578820781474
1106	30-31	0.18106428060786328
1106	32-33	0.1684042273202735
1106	34-35	0.1291920546157428
1106	36-37	0.1053293815723606
1106	38-39	0.11507679538085114
1106	40-41	0.1498685684186185
1106	42-43	0.18133243111198283
1106	44-45	0.15641906377827297
1106	46-47	0.13332963030256906
1106	48-49	0.1734207456630017
1106	50-51	0.12674310098888952
1106	52-53	0.06989543830139411
1106	54-55	0.10131480263071779
1106	56-57	0.07391114730100412
1106	58-59	0.11747911962808644
1106	60-61	0.08843568088183673
1106	62-63	0.11210496578257079
1106	64-65	0.11202884355049036
1106	66-67	0.05629315634662646
1106	68-69	0.03316142994318483
1106	70-71	0.032032000580827
1106	72-73	0.057779525831207934
1106	74-75	0.09653583540207933
1106	76-77	0.034691961316738684
1106	78-79	0.07937505704259706
1106	80-81	0.09251947672542826
1106	82-83	0.08021670839330142
1106	84-85	0.06581842526432524
1106	86-87	0.04830090831160305
1106	88-89	0.09941777155462006
1106	90-91	0.03331130526438386
1106	92-93	0.09929390935764815
1106	94-95	0.09241260087176784
1106	96-97	0.08530275827870071
1106	98-99	0.08408618443210258
1106	100	0.16372064894807892
1107	1	0.13208675674890458
1107	2	0.08577596332139592
1107	3	0.1243454300036646
1107	4	0.10629666392397041
1107	5	0.12054285979859714
1107	6	0.12997167142232513
1107	7	0.11912547157124465
1107	8	0.09707725484309293
1107	9	0.14814966425475262
1107	10-11	0.13846398541839022
1107	12-13	0.1721101316490561
1107	14-15	0.22364239098595817
1107	16-17	0.24964012526635315
1107	18-19	0.23356243509382324
1107	20-21	0.1624810893058921
1107	22-23	0.1961861287589386
1107	24-25	0.18303332815712992
1107	26-27	0.1937442895244672
1107	28-29	0.2183305806894964
1107	30-31	0.24312191255589255
1107	32-33	0.2234186474005071
1107	34-35	0.21533747115163493
1107	36-37	0.21482780988997519
1107	38-39	0.2266126264463395
1107	40-41	0.2094698765852101
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2216	46-47	-0.41385555484803405
2216	48-49	-0.3244446771369809
2216	50-51	-0.35270979406537606
2216	52-53	-0.3934592434495343
2216	54-55	-0.367727058351214
2216	56-57	-0.4053443472553653
2216	58-59	-0.4372056737406069
2216	60-61	-0.4630442976234619
2216	62-63	-0.4338942912713293
2216	64-65	-0.38871323105568223
2216	66-67	-0.3831877291318264
2216	68-69	-0.3357434279812139
2216	70-71	-0.41007044661265013
2216	72-73	-0.34649165167299145
2216	74-75	-0.3341687853211397
2216	76-77	-0.36222404982527223
2216	78-79	-0.312913850323973
2216	80-81	-0.3370394134245345
2216	82-83	-0.32583041219258035
2216	84-85	-0.3025932776773743
2216	86-87	-0.24047403053229033
2216	88-89	-0.18004103718873665
2216	90-91	-0.29619613579053095
2216	92-93	-0.2827880017723672
2216	94-95	-0.2769982820902541
2216	96-97	-0.2705437248426179
2216	98-99	-0.2607047892241283
2216	100	-0.3941829420462426
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	27.0
8	140.0
9	316.0
10	315.0
11	238.0
12	210.0
13	318.0
14	559.0
15	1085.0
16	2040.0
17	3609.0
18	6324.0
19	9523.0
20	13906.0
21	20309.0
22	28875.0
23	34221.0
24	32170.0
25	28083.0
26	26796.0
27	30367.0
28	37445.0
29	46237.0
30	56952.0
31	71815.0
32	92596.0
33	120966.0
34	173525.0
35	266728.0
36	474327.0
37	1087003.0
38	1577752.0
39	580885.0
40	1692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.908068750186445	20.028107298285388	13.506335743003836	24.557488208524333
2	14.979320117129033	20.913788763774935	40.34330780760096	23.763583311495072
3	17.590103876975853	26.46330436295728	30.977234898272236	24.96935686179463
4	11.990103895829348	18.125489514975246	39.69011771986697	30.19428886932844
5	12.52006583478948	38.37961068810613	35.838404386776276	13.261919090328107
6	29.094620333276573	39.218168582308806	18.4095343334365	13.277676750978124
7	25.24681222881106	33.435169660232084	23.623169131578088	17.694848979378765
8	24.99037771956304	35.56361704939658	21.106394455909243	18.33961077513114
9	25.952618734374195	14.931004602731395	21.1390677827239	37.97730888017051
10-11	24.042275342200995	26.505281465846807	29.295288743126928	20.157154448825267
12-13	25.039861373360772	24.362823948104086	28.950636528699462	21.646678149835676
14-15	22.319921257061452	25.401640878381915	27.035589997707852	25.242847866848784
16-17	21.3978853578103	28.13141560422025	27.819142275308263	22.651556762661187
18-19	21.19199282338354	27.339078451931492	30.07608013059768	21.392848594087287
20-21	22.315652970552637	26.48931580885342	29.7635085971215	21.43152262347244
22-23	22.14893041841754	26.41871169615659	29.459476255630673	21.9728816297952
24-25	21.846352712820995	26.522391661686367	29.8647085205045	21.766547104988135
26-27	21.839993362809174	26.748427991502986	29.50006561579883	21.911513029889008
28-29	21.5842548957198	26.940066241250772	29.601130391397607	21.874548471631822
30-31	22.123084441145643	26.618817032842795	29.48176787465655	21.776330651355007
32-33	22.024223892177506	26.941909951885524	29.105771779353084	21.928094376583882
34-35	22.077563729887633	26.832567332798675	29.53357501890531	21.556293918408382
36-37	22.165866279705078	26.639633215100822	29.364750139957668	21.829750365236432
38-39	22.040161211620653	26.712371120245926	29.517789323868037	21.729678344265384
40-41	22.144748975598205	26.41670720840581	29.569143767791278	21.869400048204707
42-43	22.08702281062818	26.537399880472844	29.139352709713705	22.236224599185267
44-45	22.1261682847997	26.97501565308719	28.981883849691002	21.916932212422104
46-47	22.353607720998355	26.728187589269904	28.517117551951326	22.401087137780422
48-49	22.567068622839795	26.765541470419436	28.343964251197423	22.323425655543346
50-51	22.361924905046347	26.975051555147694	28.07765329046652	22.58537024933944
52-53	22.126135188628346	27.17503741452455	28.077408732538117	22.621418664308983
54-55	22.161162505379522	27.116549357227825	27.997988537147585	22.72429960024506
56-57	22.460595596235823	27.026819593990282	27.85174466930338	22.66084014047052
58-59	22.118272129038978	27.093551872971243	28.21286940684333	22.575306591146447
60-61	22.298850812820763	27.222530076180906	27.924658066320372	22.553961044677955
62-63	22.214862532152754	26.949409271800846	27.937187859086986	22.89854033695941
64-65	21.81938443895349	27.00218091303361	28.033681517093484	23.144753130919412
66-67	22.20383602234959	26.889661947112376	27.890222924157065	23.016279106380967
68-69	21.95734431380949	26.829129597909528	28.05174474121777	23.161781347063208
70-71	22.030041092246666	26.616707283826525	28.00172688530051	23.3515247386263
72-73	22.223966965518404	26.59108590624562	28.04140952562858	23.143537602607392
74-75	22.091327805854117	26.938905047847978	27.87493121034269	23.09483593595521
76-77	22.056576582586143	26.78836540530171	27.716689711720516	23.43836830039163
78-79	21.919730084469787	26.827993274898866	27.74958734593834	23.502689294693006
80-81	21.768881944132993	26.97549470423809	27.935140187477337	23.320483164151582
82-83	22.042900381847247	26.526389470934514	28.208077230382138	23.2226329168361
84-85	22.22538132139629	26.71375692399446	27.620397671547114	23.440464083062142
86-87	21.641478615100816	26.9800646523821	27.856144824650354	23.52231190786673
88-89	21.939012150546212	26.560538845806864	27.737477355611922	23.762971648035
90-91	21.84556422536326	26.42534117558836	27.880351820138284	23.848742778910097
92-93	21.877496839365175	26.584921384452308	27.716959998574787	23.820621777607727
94-95	21.33100755787258	26.530588570272656	27.98522709299853	24.153176778856228
96-97	21.478757335566172	26.40657778812625	27.907156678723695	24.207508197583884
98-99	21.39979048550644	26.623359270954193	27.745755384267685	24.23109485927168
100	21.692136673534314	26.35435657567343	27.46631030632422	24.487196444468033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	130.0
2	198.5
3	323.5
4	231.5
5	217.5
6	444.5
7	1138.0
8	2164.0
9	2749.0
10	2301.0
11	1762.0
12	1891.5
13	2154.5
14	2545.0
15	3088.0
16	3653.0
17	4398.5
18	5145.0
19	5882.5
20	6853.5
21	8005.0
22	9889.5
23	12593.0
24	15333.5
25	18930.0
26	24977.5
27	32282.5
28	39923.5
29	49171.5
30	58536.0
31	66700.5
32	78507.5
33	90518.5
34	100598.5
35	111095.0
36	122740.5
37	136721.0
38	144587.0
39	145827.5
40	146196.0
41	148638.5
42	153727.0
43	162195.5
44	173444.5
45	186788.5
46	195425.0
47	200314.0
48	205086.0
49	201481.0
50	193321.0
51	189304.0
52	183820.5
53	174600.5
54	164233.0
55	146790.0
56	130072.0
57	112788.0
58	94828.0
59	83010.5
60	68422.0
61	52363.0
62	39791.0
63	29966.5
64	21605.0
65	14579.5
66	10347.0
67	7643.5
68	5530.5
69	4174.5
70	3206.5
71	2287.5
72	1738.0
73	1484.0
74	1156.0
75	894.5
76	681.5
77	421.0
78	267.0
79	181.0
80	118.0
81	66.5
82	36.0
83	13.5
84	4.0
85	3.0
86	1.0
87	0.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0028794236181097104
2	0.004143055565625482
3	2.071527782812741E-4
4	0.0502552640110371
5	0.0024858333393752894
6	0.0052409652905162345
7	2.071527782812741E-5
8	3.52159723078166E-4
9	8.286111131250964E-5
10-11	4.143055565625482E-5
12-13	0.0
14-15	3.1072916742191115E-5
16-17	2.7965625067972007E-4
18-19	2.1751041719533782E-4
20-21	2.589409728515926E-4
22-23	0.0
24-25	0.0
26-27	2.3822569502346522E-4
28-29	1.0357638914063705E-5
30-31	2.1751041719533782E-4
32-33	2.7965625067972007E-4
34-35	1.76079861539083E-4
36-37	5.178819457031852E-5
38-39	7.353923628985232E-4
40-41	5.282395846172489E-4
42-43	0.0
44-45	6.732465294141408E-4
46-47	0.0
48-49	9.321875022657336E-5
50-51	0.0
52-53	3.832326398203571E-4
54-55	4.6609375113286673E-4
56-57	1.5536458371095559E-4
58-59	7.975381963829053E-4
60-61	6.214583348438223E-4
62-63	1.657222226250193E-4
64-65	3.935902787344208E-4
66-67	0.001305062503172027
68-69	0.002496190978289353
70-71	0.0034594513972972776
72-73	0.00324194098010194
74-75	0.002889781257023774
76-77	0.004484857649789584
78-79	0.0013879236144845365
80-81	0.0010668368081485616
82-83	6.628888905000771E-4
84-85	6.111006959297586E-4
86-87	0.0
88-89	0.0
90-91	4.143055565625482E-5
92-93	4.143055565625482E-5
94-95	9.321875022657336E-5
96-97	3.7287500090629343E-4
98-99	8.286111131250964E-5
100	0.07815874324552471
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4827355.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.06865006699467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.30083095188112	33.85893935032586
2	16.460114881600987	16.811916939020243
3	7.021470170919596	10.757310093436022
4	3.5878355931596135	7.329036816199062
5	2.1055566252624756	5.37639672458858
6	1.257895606432691	3.854341832743268
7	0.8324832839047381	2.9759658258647548
8	0.5528864487626173	2.2588131662913185
9	0.39103145879701895	1.7972503861042262
>10	1.4567423211197028	11.306188307371738
>50	0.022528381029944238	0.7478976801226173
>100	0.008651297673301602	0.8472058161975401
>500	0.0010230263848071974	0.3338428435367942
>1k	7.307331318835354E-4	0.58693049881988
>5k	7.307331318835353E-5	0.24752733969177673
>10k+	1.4614662637670706E-4	0.9104363796864581
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12553	0.2600388825764834	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11844	0.24535175059634107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6633	0.13740443783396913	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.143055565625482E-5	8.286111131250964E-5
2	0.0	0.0	0.0	1.657222226250193E-4	8.286111131250964E-5
3	0.0	0.0	0.0	1.657222226250193E-4	8.286111131250964E-5
4	0.0	0.0	0.0	1.8643750045314671E-4	8.286111131250964E-5
5	0.0	0.0	0.0	2.2786805610940152E-4	8.286111131250964E-5
6	0.0	0.0	0.0	2.2786805610940152E-4	1.2429166696876446E-4
7	0.0	0.0	0.0	2.2786805610940152E-4	1.2429166696876446E-4
8	0.0	0.0	0.0	2.2786805610940152E-4	1.4500694479689189E-4
9	0.0	0.0	0.0	2.485833339375289E-4	1.4500694479689189E-4
10-11	0.0	0.0	0.0	3.1072916742191117E-4	1.657222226250193E-4
12-13	0.0	0.0	0.0	3.5215972307816603E-4	6.628888905000771E-4
14-15	0.0	0.0	0.0	3.9359027873442083E-4	8.078958352969691E-4
16-17	0.0	0.0	0.0	3.9359027873442083E-4	9.632604190079247E-4
18-19	0.0	0.0	0.0	4.0394791764848453E-4	0.0011186250027188803
20-21	0.0	0.0	0.0	4.1430555656254823E-4	0.0013982812533986002
22-23	0.0	0.0	0.0	4.7645139004693043E-4	0.0014707847257970463
24-25	0.0	0.0	0.0	6.732465294141408E-4	0.0014707847257970463
26-27	0.0	0.0	0.0	0.0014604270868829824	0.0015743611149376833
28-29	0.0	0.0	0.0	0.004733440983727114	0.0016157916705939381
30-31	0.0	0.0	0.0	0.014956430591907991	0.0016157916705939381
32-33	0.0	0.0	0.0	0.04056051398747347	0.001688295142992384
34-35	0.0	0.0	0.0	0.08184606269893141	0.0017400833375627025
36-37	0.0	0.0	0.0	0.149087854529033	0.0017400833375627025
38-39	0.0	0.0	0.0	0.24813795546422418	0.0018125868099611484
40-41	0.0	0.0	0.0	0.35255331335690043	0.0018229444488752121
42-43	0.0	0.0	0.0	0.460769924731038	0.0018540173656174032
44-45	0.0	0.0	0.0	0.5991583382618432	0.0019575937547580404
46-47	0.0	0.0	0.0	0.7626950990759951	0.002081885421726805
48-49	0.0	0.0	0.0	0.9406704085363518	0.002268322922179952
50-51	0.0	0.0	0.0	1.1249638777342872	0.0023822569502346524
52-53	0.0	0.0	0.0	1.307838350400996	0.002610125006344054
54-55	0.0	0.0	0.0	1.5093565731130194	0.002661913200914372
56-57	0.0	0.0	0.0	1.7527507299546023	0.0027240590343987547
58-59	0.0	0.0	0.0	2.0291236090985643	0.0027551319511409456
60-61	0.0	0.0	0.0	2.3185574709131607	0.0027551319511409456
62-63	0.0	0.0	0.0	2.608674936896085	0.003003715285078475
64-65	0.0	0.0	0.0	2.909139684154159	0.0030140729239925383
66-67	0.0	0.0	0.0	3.2525161294331992	0.003055503479648793
68-69	0.0	0.0	0.0	3.6405754289875096	0.0030865763963909844
70-71	0.0	0.0	0.0	4.055719954302097	0.0031383645909613027
72-73	0.0	0.0	0.0	4.471444507395872	0.0032937291746722584
74-75	0.0	0.0	0.0	4.892679324391929	0.0034283784805550863
76-77	0.0	0.0	0.0	5.35475431162614	0.003480166675125405
78-79	0.0	0.0	0.0	5.86383226425237	0.0035008819529535324
80-81	0.0	0.0	0.0	6.406593258627137	0.0035423125086097873
82-83	0.0	0.0	0.0	6.966392569015538	0.0037494652868910613
84-85	0.0	0.0	0.0	7.523322813424743	0.0037908958425473165
86-87	0.0	0.0	0.0	8.106219658591506	0.003956618065172336
88	0.0	0.0	0.0	8.563032965257372	0.004122340287797355
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7990	0.0	59.795143	1
GTATCAA	14975	0.0	41.544353	1
ATCAACG	17875	0.0	33.618732	3
CAACGCA	18130	0.0	33.132835	5
TATCAAC	19030	0.0	32.271145	2
AACGCAG	19365	0.0	31.406519	6
TCAACGC	19525	0.0	30.873722	4
CGCAGAG	21180	0.0	28.42521	8
ACGCAGA	21165	0.0	28.423153	7
GCAGAGT	24185	0.0	24.795942	9
GTGGTAT	2555	0.0	24.110123	1
GTACATG	13545	0.0	22.357635	1
GAGTACT	17385	0.0	22.356655	12-13
TACATGG	13110	0.0	22.130566	2
CAGAGTA	23930	0.0	22.084652	10-11
GTGATCG	1405	0.0	21.742905	8
AGAGTAC	22840	0.0	21.543901	10-11
ACATGGG	13145	0.0	21.24821	3
AGTACTT	18185	0.0	20.416903	12-13
TGATCGC	1505	0.0	20.297983	9
>>END_MODULE