FastQCFastQC Report
Mon 16 Jan 2017
ERR1146586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5102530
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74250.037.1244741
GGTATCA51700.036.757841
CAACGCA80850.032.1591035
ATCAACG85000.030.64613
GTACTAG18750.029.1015321
AACGCAG93300.028.81486
TATCAAC97250.028.0915182
TCAACGC98150.026.7786624
GTACATG176450.026.1254371
TACATGG172750.025.6674252
ACGCAGA102100.025.2709887
CGCAGAG106550.024.12648
ACATGGG179150.024.0940763
CATGGGG141400.022.5448654
GTGATCG15950.020.330458
TGATCGC15500.020.0105069
CTAGGCA33050.019.3479444
GGGCTAT35000.019.3372926
GCAGAGT129850.019.3261419
ATGGGGG94200.019.2661635
Produced by FastQC (version 0.11.5)