##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146586_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5102530
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.128276756824555	31.0	31.0	34.0	28.0	34.0
2	31.409507048464192	33.0	31.0	34.0	28.0	34.0
3	31.505415352775977	33.0	31.0	34.0	28.0	34.0
4	35.00061420511001	37.0	35.0	37.0	32.0	37.0
5	34.98700174227295	37.0	35.0	37.0	32.0	37.0
6	35.10561035407925	37.0	35.0	37.0	32.0	37.0
7	35.014225100097406	37.0	35.0	37.0	32.0	37.0
8	34.99273046900263	37.0	35.0	37.0	32.0	37.0
9	36.5886658187213	39.0	37.0	39.0	32.0	39.0
10-11	36.578040207504905	39.0	37.0	39.0	32.0	39.0
12-13	36.51945123301578	39.0	37.0	39.0	32.0	39.0
14-15	37.86545752793222	40.0	38.0	41.0	32.0	41.0
16-17	37.89322336174408	40.0	38.0	41.0	32.0	41.0
18-19	37.91287841521755	40.0	38.0	41.0	32.0	41.0
20-21	38.010099107697556	40.0	38.0	41.0	32.0	41.0
22-23	38.02065759534976	40.0	38.0	41.0	32.0	41.0
24-25	38.0421432113089	40.0	38.0	41.0	32.0	41.0
26-27	38.10516087117567	40.0	38.0	41.0	32.0	41.0
28-29	38.08772471695414	40.0	38.0	41.0	32.0	41.0
30-31	38.054750388532746	40.0	38.0	41.0	32.0	41.0
32-33	38.026325175942134	40.0	38.0	41.0	32.0	41.0
34-35	37.98031623527936	40.0	38.0	41.0	32.0	41.0
36-37	37.907060125075205	40.0	38.0	41.0	31.0	41.0
38-39	37.85177421788799	40.0	38.0	41.0	31.0	41.0
40-41	37.79668350798526	40.0	38.0	41.0	31.0	41.0
42-43	37.64608086576659	40.0	37.5	41.0	31.0	41.0
44-45	37.53088938232602	40.0	37.0	41.0	31.0	41.0
46-47	37.421834952464756	40.0	37.0	41.0	30.0	41.0
48-49	37.31355161067157	40.0	36.5	41.0	30.0	41.0
50-51	37.18084371870425	40.0	36.0	41.0	30.0	41.0
52-53	37.04657660023557	40.0	36.0	41.0	30.0	41.0
54-55	36.9242180839701	39.0	35.0	41.0	30.0	41.0
56-57	36.73284556876687	39.0	35.0	41.0	30.0	41.0
58-59	36.53264850966089	39.0	35.0	41.0	30.0	41.0
60-61	36.31538746464989	38.5	35.0	40.5	30.0	41.0
62-63	36.07531655864835	38.0	35.0	40.0	29.5	41.0
64-65	35.7782313871746	37.0	35.0	40.0	29.0	41.0
66-67	35.536854560384754	37.0	34.5	40.0	29.0	41.0
68-69	35.27617799405393	36.0	34.0	39.5	29.0	41.0
70-71	34.99691633366193	36.0	34.0	39.0	29.0	41.0
72-73	34.6610672548716	35.0	34.0	39.0	28.0	41.0
74-75	34.3302582248414	35.0	34.0	37.5	28.0	40.0
76-77	34.06191212986499	35.0	34.0	37.0	28.0	39.0
78-79	33.761011792189365	35.0	34.0	37.0	27.0	39.0
80-81	33.52834270450149	35.0	34.0	36.0	27.0	39.0
82-83	33.262460681269886	35.0	33.0	36.0	27.0	37.0
84-85	33.06529123787611	35.0	33.0	35.5	27.0	37.0
86-87	32.87084897100066	35.0	33.0	35.0	26.5	37.0
88-89	32.70092111168381	35.0	33.0	35.0	26.0	36.0
90-91	32.558816410682546	35.0	33.0	35.0	26.0	36.0
92-93	32.409827673722646	35.0	33.0	35.0	26.0	36.0
94-95	32.29271763223342	35.0	33.0	35.0	25.0	36.0
96-97	32.201776765643714	35.0	33.0	35.0	25.0	35.0
98-99	32.09127824824156	35.0	33.0	35.0	25.0	35.0
100	30.949306912453235	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35014738578065163
1101	2	0.3924653554896693
1101	3	0.39253893806052176
1101	4	0.43924925020749583
1101	5	0.3470651965541123
1101	6	0.41861039068152905
1101	7	0.45080267824513953
1101	8	0.43837618970859893
1101	9	0.6137829951695082
1101	10-11	0.5802863088833092
1101	12-13	0.6118248726314164
1101	14-15	0.6714300828155686
1101	16-17	0.6680622318212102
1101	18-19	0.6595409066783091
1101	20-21	0.6628554709586396
1101	22-23	0.6375533772659168
1101	24-25	0.6724577979039736
1101	26-27	0.6357786423934542
1101	28-29	0.6075739925133021
1101	30-31	0.6838712688647917
1101	32-33	0.6785354226760774
1101	34-35	0.5906392736046016
1101	36-37	0.6540231261076173
1101	38-39	0.6166022161517475
1101	40-41	0.6189102407998632
1101	42-43	0.5882069609024256
1101	44-45	0.5089296470653153
1101	46-47	0.48868609540829056
1101	48-49	0.49716794468096026
1101	50-51	0.4911740704793317
1101	52-53	0.5184254650330686
1101	54-55	0.4807596002599226
1101	56-57	0.4820513269413311
1101	58-59	0.4794772039793287
1101	60-61	0.5224512803919339
1101	62-63	0.473751082468965
1101	64-65	0.49640617454008407
1101	66-67	0.5100067635240606
1101	68-69	0.4425268799854436
1101	70-71	0.4487168946114153
1101	72-73	0.4662527102320695
1101	74-75	0.4536865021920775
1101	76-77	0.45225073504447977
1101	78-79	0.5147583081460212
1101	80-81	0.4752858129883961
1101	82-83	0.4509676281610737
1101	84-85	0.3990898424723639
1101	86-87	0.4358402830694743
1101	88-89	0.3897493460204231
1101	90-91	0.37360996839701954
1101	92-93	0.35872185280212676
1101	94-95	0.40350148253854456
1101	96-97	0.3950464026366518
1101	98-99	0.3926771488620915
1101	100	0.44816203747612704
1102	1	0.28579629176947563
1102	2	0.34407557382039045
1102	3	0.34992889981873887
1102	4	0.30951560703871905
1102	5	0.31437197228171243
1102	6	0.4152890988351672
1102	7	0.35143239790429703
1102	8	0.3523464301232977
1102	9	0.46024041085826184
1102	10-11	0.4353009522027804
1102	12-13	0.4176206438849448
1102	14-15	0.5204499113339693
1102	16-17	0.48332329489814185
1102	18-19	0.48197688703221075
1102	20-21	0.5234706427660925
1102	22-23	0.47528232844101836
1102	24-25	0.556844306418661
1102	26-27	0.5225426117688201
1102	28-29	0.5286351789184423
1102	30-31	0.581636462827035
1102	32-33	0.539354550362539
1102	34-35	0.545599446518672
1102	36-37	0.5564578810678853
1102	38-39	0.5362273747828539
1102	40-41	0.530859097154412
1102	42-43	0.5426970783761789
1102	44-45	0.5438287367511379
1102	46-47	0.508022977273626
1102	48-49	0.5033195865605151
1102	50-51	0.49681332391610056
1102	52-53	0.4590254050273259
1102	54-55	0.3734146472905664
1102	56-57	0.4711222410177527
1102	58-59	0.4133770555247338
1102	60-61	0.4513666393968663
1102	62-63	0.4399550263360794
1102	64-65	0.5329641621479198
1102	66-67	0.4901343434056997
1102	68-69	0.39171788712005906
1102	70-71	0.3741304246244539
1102	72-73	0.3765162469547434
1102	74-75	0.3789733021209756
1102	76-77	0.36819130892133245
1102	78-79	0.41251202804936327
1102	80-81	0.3610019228987653
1102	82-83	0.408395620149669
1102	84-85	0.33346846705362054
1102	86-87	0.31044981770764224
1102	88-89	0.3279403163125636
1102	90-91	0.3202774893432263
1102	92-93	0.36071307263711816
1102	94-95	0.4116743964268963
1102	96-97	0.37028018434573085
1102	98-99	0.3661039679867315
1102	100	0.4925038966627113
1103	1	0.18426409175068414
1103	2	0.2032933745924126
1103	3	0.19854546218801516
1103	4	0.25383129877121036
1103	5	0.25981211291455253
1103	6	0.2430001876667589
1103	7	0.30036433602331414
1103	8	0.31170964622583597
1103	9	0.36332663730179604
1103	10-11	0.34097405231587885
1103	12-13	0.31398351656142154
1103	14-15	0.3944649338515873
1103	16-17	0.4203513352040744
1103	18-19	0.40745505673632465
1103	20-21	0.39015864425299185
1103	22-23	0.36264520701652714
1103	24-25	0.4011242705559894
1103	26-27	0.38198034371713874
1103	28-29	0.33415192057544374
1103	30-31	0.3952007619399325
1103	32-33	0.45980738886824923
1103	34-35	0.4338346332669545
1103	36-37	0.40103707129539856
1103	38-39	0.36756766999589274
1103	40-41	0.3259257950773886
1103	42-43	0.3475100402690501
1103	44-45	0.2744756481693571
1103	46-47	0.28594867960642034
1103	48-49	0.2968718798677443
1103	50-51	0.3251073479179638
1103	52-53	0.2961593585452036
1103	54-55	0.24831215166802423
1103	56-57	0.3380524232780786
1103	58-59	0.3337079166521093
1103	60-61	0.31693953211208736
1103	62-63	0.3105341425265067
1103	64-65	0.21080467908749512
1103	66-67	0.24011410289802626
1103	68-69	0.23266385278720492
1103	70-71	0.22037782167807052
1103	72-73	0.26471845471323974
1103	74-75	0.2606813846707894
1103	76-77	0.19553952199868974
1103	78-79	0.25769159405355424
1103	80-81	0.2172536450517839
1103	82-83	0.21078500032505332
1103	84-85	0.2308458444155903
1103	86-87	0.2420261937565229
1103	88-89	0.16293659864212628
1103	90-91	0.22197077559033573
1103	92-93	0.19694404730796577
1103	94-95	0.2096317679941464
1103	96-97	0.22666343773867936
1103	98-99	0.12210851656527666
1103	100	0.24674480666567788
1104	1	0.19523825456785104
1104	2	0.22032720294741637
1104	3	0.20692245315715496
1104	4	0.26168223081905495
1104	5	0.24731465079257475
1104	6	0.29243960542591196
1104	7	0.3676384168309923
1104	8	0.31524762709110377
1104	9	0.4918694179001193
1104	10-11	0.40458154599239293
1104	12-13	0.47393992128635887
1104	14-15	0.5380264216522903
1104	16-17	0.5213674576045193
1104	18-19	0.47535288815661403
1104	20-21	0.46432603113518667
1104	22-23	0.5164327433314355
1104	24-25	0.49471941271498565
1104	26-27	0.47654639162205115
1104	28-29	0.47580843061847844
1104	30-31	0.4958317913809509
1104	32-33	0.5025503560581086
1104	34-35	0.45950003779295656
1104	36-37	0.47398350627450725
1104	38-39	0.4647103107016761
1104	40-41	0.47924213970701146
1104	42-43	0.5012781936364235
1104	44-45	0.4731596114587049
1104	46-47	0.43142685058405306
1104	48-49	0.418564444646762
1104	50-51	0.43393419501779107
1104	52-53	0.378402605540586
1104	54-55	0.37960907940950506
1104	56-57	0.4238679802198817
1104	58-59	0.38800884550012427
1104	60-61	0.3920044537409808
1104	62-63	0.3862116818408694
1104	64-65	0.45630614844986184
1104	66-67	0.40434227004200807
1104	68-69	0.37650157825964214
1104	70-71	0.3463101199865406
1104	72-73	0.41363826431053496
1104	74-75	0.3946057547812174
1104	76-77	0.3125649367641188
1104	78-79	0.33468097861839397
1104	80-81	0.28843897158799336
1104	82-83	0.29067377123967475
1104	84-85	0.27817060917755043
1104	86-87	0.27208793907644946
1104	88-89	0.34887831853522755
1104	90-91	0.34220836186541703
1104	92-93	0.25661865300913433
1104	94-95	0.3023992296683531
1104	96-97	0.3062927777145603
1104	98-99	0.3194118537649757
1104	100	0.372848402795384
1105	1	0.1441980315976572
1105	2	0.19904266016010652
1105	3	0.16923492150078445
1105	4	0.16546129366161466
1105	5	0.11192111988052744
1105	6	0.2050895790073639
1105	7	0.2571683541411218
1105	8	0.2818548373823475
1105	9	0.2941092147369986
1105	10-11	0.2782517929286783
1105	12-13	0.2804037958269632
1105	14-15	0.3882680254162594
1105	16-17	0.34637895836154087
1105	18-19	0.345106317002859
1105	20-21	0.34341323225707043
1105	22-23	0.3269277991774757
1105	24-25	0.395889502467746
1105	26-27	0.3684019225879851
1105	28-29	0.34994218344632344
1105	30-31	0.4197221635876218
1105	32-33	0.401724556794548
1105	34-35	0.36483780467788307
1105	36-37	0.37586393862486744
1105	38-39	0.40655355011151073
1105	40-41	0.3743329371097488
1105	42-43	0.38412005022178164
1105	44-45	0.34795716689372114
1105	46-47	0.3476848400955177
1105	48-49	0.3219035986518577
1105	50-51	0.34226161200243865
1105	52-53	0.2573464474807423
1105	54-55	0.2724450157410061
1105	56-57	0.2777274214432026
1105	58-59	0.260488539537036
1105	60-61	0.38541938191885095
1105	62-63	0.3369292197338112
1105	64-65	0.377149132460886
1105	66-67	0.3701393213182058
1105	68-69	0.37734270535726466
1105	70-71	0.33081037987631845
1105	72-73	0.27684814316793194
1105	74-75	0.29183515602721144
1105	76-77	0.27163678857345275
1105	78-79	0.2954761573604543
1105	80-81	0.24953568885408828
1105	82-83	0.2958303182164528
1105	84-85	0.31199737184918064
1105	86-87	0.2828518568474081
1105	88-89	0.23760155291518004
1105	90-91	0.21575990107284326
1105	92-93	0.2660011228875021
1105	94-95	0.31651507138513324
1105	96-97	0.2729423592158753
1105	98-99	0.2832750595824507
1105	100	0.39453295323684756
1106	1	0.12014972566578663
1106	2	0.19834698969238218
1106	3	0.21671982092239261
1106	4	0.22833366001025723
1106	5	0.10845233353568773
1106	6	0.18132797905328601
1106	7	0.22071885086813126
1106	8	0.1883518668491817
1106	9	0.25765805019409527
1106	10-11	0.27262409505373597
1106	12-13	0.278957231358703
1106	14-15	0.37785632658558654
1106	16-17	0.3349916194435707
1106	18-19	0.3402782923944443
1106	20-21	0.38439704396676433
1106	22-23	0.3640105931457569
1106	24-25	0.3702132980671493
1106	26-27	0.37769661821521794
1106	28-29	0.3443382179986898
1106	30-31	0.3219903848524197
1106	32-33	0.3843905653062407
1106	34-35	0.3517252460834328
1106	36-37	0.38238087229594697
1106	38-39	0.35568335684996555
1106	40-41	0.36441990221644005
1106	42-43	0.37451572856867443
1106	44-45	0.34271039273302506
1106	46-47	0.3694842612141471
1106	48-49	0.39744836009094087
1106	50-51	0.36960141391918455
1106	52-53	0.3128946672821584
1106	54-55	0.31971262653474497
1106	56-57	0.340125057094653
1106	58-59	0.29892288611357287
1106	60-61	0.3596335884711408
1106	62-63	0.2693634190232501
1106	64-65	0.28876714509573276
1106	66-67	0.3135807025513131
1106	68-69	0.32067195999912457
1106	70-71	0.26018033942822427
1106	72-73	0.28893693048689073
1106	74-75	0.32839380108232064
1106	76-77	0.3027134346016638
1106	78-79	0.2863669489084799
1106	80-81	0.21732341879483386
1106	82-83	0.25955540345785977
1106	84-85	0.2370716981604204
1106	86-87	0.18079550956590396
1106	88-89	0.18680483889977495
1106	90-91	0.19956945659514957
1106	92-93	0.17603638343267392
1106	94-95	0.17948022057118607
1106	96-97	0.19142970468399767
1106	98-99	0.2005622470729378
1106	100	0.31303274910145973
1107	1	0.06825133830324148
1107	2	0.1392071759832767
1107	3	0.09643085969987197
1107	4	0.1183731691481853
1107	5	0.15657711293886223
1107	6	0.22005713698898433
1107	7	0.1772207458695263
1107	8	0.12277471582430621
1107	9	0.2019944076095186
1107	10-11	0.2373279121649503
1107	12-13	0.22518473307084008
1107	14-15	0.2672021529677977
1107	16-17	0.2414643885879144
1107	18-19	0.20523340091824593
1107	20-21	0.294197757663305
1107	22-23	0.31460437612742
1107	24-25	0.36864012729418505
1107	26-27	0.27520866910976594
1107	28-29	0.2764514315528501
1107	30-31	0.24042922683443635
1107	32-33	0.30246841889297116
1107	34-35	0.30654623898367817
1107	36-37	0.282421049998014
1107	38-39	0.24805735252821393
1107	40-41	0.26219783420155096
1107	42-43	0.25373675159399056
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2216	60-61	-0.542679715277707
2216	62-63	-0.603476733841326
2216	64-65	-0.567005303242972
2216	66-67	-0.5080042121101656
2216	68-69	-0.5475226660592369
2216	70-71	-0.5474332450642905
2216	72-73	-0.5299070439922033
2216	74-75	-0.5187961024420744
2216	76-77	-0.4896471545481731
2216	78-79	-0.502344487448859
2216	80-81	-0.5140646578365136
2216	82-83	-0.44607216845267317
2216	84-85	-0.4434504661741556
2216	86-87	-0.507581814377275
2216	88-89	-0.45668267178255917
2216	90-91	-0.45004773843932355
2216	92-93	-0.37206906876025414
2216	94-95	-0.4565180754040199
2216	96-97	-0.4483335765847727
2216	98-99	-0.4269985092189188
2216	100	-0.5309747637087554
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1506.0
1	438.0
2	298.0
3	271.0
4	212.0
5	288.0
6	378.0
7	722.0
8	2072.0
9	3277.0
10	3799.0
11	4902.0
12	6550.0
13	8507.0
14	10811.0
15	12999.0
16	14095.0
17	15610.0
18	17699.0
19	20059.0
20	22548.0
21	24669.0
22	27568.0
23	32243.0
24	38999.0
25	47495.0
26	51051.0
27	47283.0
28	50929.0
29	62635.0
30	72564.0
31	83299.0
32	105364.0
33	141696.0
34	208027.0
35	322489.0
36	595220.0
37	1214014.0
38	1394952.0
39	434063.0
40	929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61676830667858	19.984023233796353	12.973196934438366	24.426011525086704
2	15.058500060794566	20.829904650588137	39.95569841188906	24.155896876728235
3	17.80240739636521	26.509114432138787	30.14001955166126	25.548458619834737
4	12.254632836082035	17.482881794004072	39.538568996633536	30.723916373280357
5	13.127665926395816	38.51877961622821	35.30036676164259	13.053187695733381
6	29.794855096059912	38.67183484834813	18.37318011871648	13.160129936875473
7	26.331856614780456	32.865654573397684	23.543732217343415	17.258756594478438
8	25.204860994643163	35.857789641325795	20.970449212225496	17.96690015180555
9	26.11928882040934	15.698109551405814	20.806937633318714	37.37566399486612
10-11	24.40950125222168	26.707279244012533	28.743825071638536	20.13939443212725
12-13	24.75837402353805	24.622344497129085	28.254230869327913	22.365050610004957
14-15	22.479271199021888	25.73486023021137	25.585833128285408	26.200035442481333
16-17	22.308005306449207	28.43746999627034	26.459732015880906	22.794792681399546
18-19	22.15192964500636	28.46734634755048	27.525575315889988	21.85514869155317
20-21	23.33168001189293	27.545982634829542	26.741199495491806	22.38113785778572
22-23	23.50434437160649	27.284003076525682	26.452462470887887	22.759190080979938
24-25	22.96168647341209	27.50312421943548	26.979624391496788	22.555564915655637
26-27	22.862095875336134	27.692773566628954	26.583221002254078	22.861909555780837
28-29	22.640533157327212	27.851693919992076	26.66207363914397	22.845699283536742
30-31	23.032390618733874	27.540180928313145	26.649014980395137	22.778413472557844
32-33	23.010862829875165	27.87880487882861	26.22265437197567	22.887677919320552
34-35	23.164681026647376	27.661635602698333	26.78335144048195	22.39033193017235
36-37	23.125332980769304	27.51741307975924	26.707862779360568	22.649391160110884
38-39	23.0709363890535	27.53203360868476	26.807257335397733	22.589772666864004
40-41	22.953413387094812	27.151665511328392	27.02696155450078	22.867959547076016
42-43	22.871161708820253	27.309227494015715	26.762856586586864	23.056754210577164
44-45	22.69533512327289	27.726291269753702	26.72678902006939	22.851584586904018
46-47	22.795833530301017	27.388518415285297	26.677326132783225	23.138321921630464
48-49	22.977966274589807	27.288863081739784	26.76882832055618	22.964342323114227
50-51	22.89007815284579	27.3399314335842	26.553273343113965	23.216717070456042
52-53	22.735309127757493	27.52309469784913	26.573882778267066	23.16771339612631
54-55	22.62626347825379	27.452733489270898	26.61582365801517	23.305179374460142
56-57	22.967901718937743	27.33333660282975	26.602712047276917	23.09604963095559
58-59	22.72445531325894	27.234734317680154	26.956580337278744	23.08423003178216
60-61	22.740783013040016	27.583505755706316	26.56753957802146	23.10817165323221
62-63	22.848838338469616	27.38311338120221	26.468568173128364	23.299480107199813
64-65	22.468766690747938	27.357284142802424	26.631461909659432	23.542487256790206
66-67	22.76803603061708	27.192466953313083	26.636261860753326	23.40323515531651
68-69	22.462567080178793	27.180566221497575	26.80945192812385	23.54741477019978
70-71	22.62295529008227	27.096389547384142	26.72406275555962	23.55659240697397
72-73	22.805674412235707	27.224615486024373	26.80662862250214	23.163081479237785
74-75	22.66312591342674	27.541490504481803	26.75008860220977	23.045294979881685
76-77	22.74611178896698	27.260945781088598	26.666226569872837	23.326715860071584
78-79	22.524353237154454	27.30292619464814	26.75555100382365	23.41716956437375
80-81	22.5391272139505	27.31473030122214	26.860024466126497	23.28611801870086
82-83	22.91676598950562	26.945153192603904	27.19983166618109	22.938249151709382
84-85	23.000735989493883	27.188607435877543	26.663536855730857	23.147119718897724
86-87	22.504686706702795	27.42491460345635	26.878086379790776	23.192312310050074
88-89	22.860331850662003	26.88737991897311	26.763484480014345	23.48880375035054
90-91	22.791253450767996	26.934244723208778	26.878902957890823	23.395598868132403
92-93	22.995635122015905	26.954813479000585	26.777832987460908	23.271718411522606
94-95	22.41659976666325	26.96372393290612	27.042719064612896	23.57695723581773
96-97	22.62926305900889	26.991434919482238	26.811761153058526	23.56754086845034
98-99	22.590304627758112	27.254779534923873	26.657040320298364	23.49787551701965
100	22.93918041476311	26.865202185131032	26.472945781544265	23.72267161856159
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3061.0
1	2188.0
2	1328.5
3	1359.5
4	1344.5
5	1191.5
6	1052.5
7	1016.0
8	1000.0
9	981.5
10	946.5
11	927.0
12	950.5
13	992.0
14	1077.5
15	1242.5
16	1400.5
17	1665.0
18	2066.0
19	2508.5
20	3317.5
21	4794.5
22	7100.0
23	9865.0
24	13426.5
25	17685.5
26	22777.0
27	29006.5
28	35235.0
29	43603.5
30	53780.0
31	63662.0
32	77089.5
33	90278.5
34	100037.0
35	109984.0
36	122187.0
37	136940.5
38	146357.5
39	149144.0
40	148832.5
41	148188.0
42	151548.0
43	160212.5
44	170332.0
45	185284.5
46	200488.0
47	208277.5
48	212652.0
49	209823.0
50	202745.5
51	202548.5
52	202673.0
53	199447.5
54	190614.5
55	170634.5
56	151868.5
57	131472.5
58	112160.5
59	101145.0
60	85729.5
61	68179.5
62	54789.0
63	42900.0
64	31487.0
65	22067.5
66	16574.5
67	12677.0
68	9336.0
69	7077.0
70	5950.5
71	4792.0
72	3633.0
73	3134.5
74	2654.0
75	2112.0
76	1572.5
77	1168.0
78	862.5
79	552.0
80	379.0
81	246.5
82	157.5
83	109.5
84	74.5
85	56.5
86	40.5
87	35.5
88	31.0
89	23.5
90	15.5
91	12.5
92	14.5
93	14.5
94	11.5
95	8.0
96	9.5
97	10.0
98	10.5
99	9.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10049916414014225
2	0.06643762996003943
3	0.08313522899424403
4	0.0843307143711061
5	0.08615333961779745
6	0.09152322475321066
7	0.08848551600872508
8	0.08938702957160467
9	0.07537437310510668
10-11	0.0821455238871697
12-13	0.0712783658302842
14-15	0.0809108422684433
16-17	0.08241989758022
18-19	0.08146938871501
20-21	0.07257184181180709
22-23	0.07685403123548515
24-25	0.07949977756132742
26-27	0.07374772906773698
28-29	0.08275306563606681
30-31	0.08688826915275363
32-33	0.08825033855753911
34-35	0.09084708958105098
36-37	0.09089608488338138
38-39	0.09173880408346448
40-41	0.08732922687372735
42-43	0.09411997577672253
44-45	0.09000437038096787
46-47	0.08625133022245826
48-49	0.09583481135828698
50-51	0.09209157026024345
52-53	0.09770643190730874
54-55	0.08916165118088479
56-57	0.09612878317226944
58-59	0.10139087864255575
60-61	0.09930367876328017
62-63	0.10059715474480307
64-65	0.10205721475424936
66-67	0.1040562230893302
68-69	0.10148886924721658
70-71	0.10309591516365411
72-73	0.1003619772936171
74-75	0.1040562230893302
76-77	0.1012536917960306
78-79	0.10914193547122701
80-81	0.10344868134043307
82-83	0.1080836369408901
84-85	0.10407582121026236
86-87	0.10061675286573524
88-89	0.09968584212145738
90-91	0.10022479044709193
92-93	0.09393379362786695
94-95	0.09304207912545345
96-97	0.09170940690206622
98-99	0.10597683894068236
100	0.2448197266846055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5102530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.71369468812941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.56121591576483	32.424427501912284
2	15.54570186973619	15.145771491900225
3	6.866309645989318	10.034499351866447
4	3.6086692937213996	7.031664568190873
5	2.089118721738928	5.088434578902266
6	1.3399871722850416	3.9165435598022027
7	0.8818218568807388	3.006976049378536
8	0.6357939990029187	2.4777499801578426
9	0.45708702863174006	2.0039758162803767
>10	1.9413746108413172	15.378774831279785
>50	0.05421548532405704	1.8027982647594618
>100	0.01830253624641086	1.4905853505983355
>500	3.214910696631141E-4	0.09870356476335104
>1k	8.037276741508357E-5	0.09909509020792096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7638308838948522E-4
2	0.0	0.0	0.0	0.0	1.7638308838948522E-4
3	0.0	0.0	0.0	0.0	1.7638308838948522E-4
4	0.0	0.0	0.0	0.0	1.7638308838948522E-4
5	0.0	0.0	0.0	0.0	2.1557933025381527E-4
6	0.0	0.0	0.0	0.0	2.9397181398247536E-4
7	0.0	0.0	0.0	0.0	2.9397181398247536E-4
8	0.0	0.0	0.0	0.0	2.9397181398247536E-4
9	0.0	0.0	0.0	1.9598120932165023E-5	2.9397181398247536E-4
10-11	0.0	0.0	0.0	1.9598120932165023E-5	3.6256523724505294E-4
12-13	0.0	9.799060466082511E-6	0.0	3.9196241864330046E-5	0.0011170928931334063
14-15	9.799060466082511E-6	1.9598120932165023E-5	0.0	3.9196241864330046E-5	0.0014796581303784593
16-17	1.9598120932165023E-5	3.9196241864330046E-5	0.0	3.9196241864330046E-5	0.0017246346420305221
18-19	1.9598120932165023E-5	7.839248372866009E-5	0.0	4.899530233041256E-5	0.0018422233676235122
20-21	3.9196241864330046E-5	7.839248372866009E-5	0.0	1.0778966512690763E-4	0.00226358296766506
22-23	3.9196241864330046E-5	8.81915441947426E-5	0.0	3.233689953807229E-4	0.002361573572325885
24-25	3.9196241864330046E-5	9.799060466082511E-5	0.0	6.369389302953633E-4	0.0023713726327919678
26-27	3.9196241864330046E-5	9.799060466082511E-5	0.0	0.0011464900745316539	0.002626148204910113
28-29	3.9196241864330046E-5	9.799060466082511E-5	0.0	0.0045957593585926985	0.002724138809570938
30-31	3.9196241864330046E-5	1.0778966512690763E-4	0.0	0.015355127750351298	0.0027633350514352684
32-33	3.9196241864330046E-5	1.2738778605907267E-4	0.0	0.038088948031662725	0.0028907228374943413
34-35	3.9196241864330046E-5	1.4698590699123768E-4	0.0	0.07404170088171946	0.0030377087444855787
36-37	3.9196241864330046E-5	1.5678496745732018E-4	0.0	0.13170917172461505	0.0030377087444855787
38-39	3.9196241864330046E-5	1.7638308838948522E-4	0.0	0.22130198156600744	0.0031063021677481565
40-41	3.9196241864330046E-5	1.9598120932165023E-4	0.0	0.32043907630136426	0.0031161012282142388
42-43	3.9196241864330046E-5	1.9598120932165023E-4	0.0	0.42367217831154347	0.0031552974700785686
44-45	3.9196241864330046E-5	2.0578026978773276E-4	0.0	0.5504132263798547	0.003292484316603724
46-47	3.9196241864330046E-5	2.1557933025381527E-4	0.0	0.7023672570273962	0.003478666465459292
48-49	3.9196241864330046E-5	2.1557933025381527E-4	0.0	0.871920400272022	0.0037236429771113546
50-51	3.9196241864330046E-5	2.1557933025381527E-4	0.0	1.0597095950440272	0.0038608298236365098
52-53	3.9196241864330046E-5	2.1557933025381527E-4	0.0	1.2450000293971812	0.004037212912025995
54-55	3.9196241864330046E-5	2.351774511859803E-4	0.0	1.4486539030637742	0.00409600727482249
56-57	3.9196241864330046E-5	2.5477557211814534E-4	0.0	1.6935912184739728	0.00417439975855115
58-59	3.9196241864330046E-5	2.5477557211814534E-4	0.0	1.9747654594877444	0.004242993181813728
60-61	3.9196241864330046E-5	2.5477557211814534E-4	0.0	2.276272750968637	0.004282189423678058
62-63	3.9196241864330046E-5	2.5477557211814534E-4	0.0	2.5840808383292213	0.004605558419058781
64-65	3.9196241864330046E-5	2.5477557211814534E-4	0.0	2.8975037873368703	0.004664352781855276
66-67	3.9196241864330046E-5	2.7437369305031035E-4	0.0	3.253141088832403	0.0046937499632535235
68-69	3.9196241864330046E-5	2.7437369305031035E-4	0.0	3.656940772518731	0.004723147144651771
70-71	3.9196241864330046E-5	3.1356993491464037E-4	0.0	4.0909215624405935	0.004791740567914348
72-73	3.9196241864330046E-5	3.5276617677897044E-4	0.0	4.5331335631539655	0.005075913321430741
74-75	3.9196241864330046E-5	3.6256523724505294E-4	0.0	4.977599347774536	0.005350287014481051
76-77	3.9196241864330046E-5	3.9196241864330046E-4	0.0	5.468796851757853	0.005497272921472289
78-79	3.9196241864330046E-5	3.9196241864330046E-4	0.0	5.99944537317762	0.005663856949395692
80-81	3.9196241864330046E-5	4.2135960004154797E-4	0.0	6.5839789281003736	0.0057716466145225995
82-83	3.9196241864330046E-5	4.801539628380431E-4	0.0	7.187346277238938	0.006104814670369405
84-85	3.9196241864330046E-5	5.095511442362907E-4	0.0	7.781247733967268	0.006134211851767653
86-87	3.9196241864330046E-5	5.291492651684557E-4	0.0	8.395893801702293	0.006418384605284046
88	3.9196241864330046E-5	5.291492651684557E-4	0.0	8.887493067164721	0.006682959237868273
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7425	0.0	37.124474	1
GGTATCA	5170	0.0	36.75784	1
CAACGCA	8085	0.0	32.159103	5
ATCAACG	8500	0.0	30.6461	3
GTACTAG	1875	0.0	29.101532	1
AACGCAG	9330	0.0	28.8148	6
TATCAAC	9725	0.0	28.091518	2
TCAACGC	9815	0.0	26.778662	4
GTACATG	17645	0.0	26.125437	1
TACATGG	17275	0.0	25.667425	2
ACGCAGA	10210	0.0	25.270988	7
CGCAGAG	10655	0.0	24.1264	8
ACATGGG	17915	0.0	24.094076	3
CATGGGG	14140	0.0	22.544865	4
GTGATCG	1595	0.0	20.33045	8
TGATCGC	1550	0.0	20.010506	9
CTAGGCA	3305	0.0	19.347944	4
GGGCTAT	3500	0.0	19.337292	6
GCAGAGT	12985	0.0	19.326141	9
ATGGGGG	9420	0.0	19.266163	5
>>END_MODULE