FastQCFastQC Report
Mon 16 Jan 2017
ERR1146586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5102530
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188260.36895422466893874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167800.32885646924172907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100400.19676513415893682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94950.058.3409541
GTATCAA171150.044.757661
CAACGCA218950.034.5818445
ATCAACG223200.033.9590643
TATCAAC230200.033.3780862
AACGCAG234250.032.6836476
TCAACGC239300.031.8118554
ACGCAGA260350.029.0635627
CGCAGAG265950.028.4515868
GTACATG162950.026.2609371
TACATGG161000.025.9359282
GCAGAGT292700.025.5302459
GTGGTAT32350.024.8567891
ACATGGG166700.024.5396613
GTACTAG20450.023.2247621
GAGTACT209000.022.95959712-13
AGAGTAC274700.022.19889610-11
CAGAGTA288150.022.1576510-11
CATGGGG134800.022.0102164
AGTACTT217700.020.91944712-13
Produced by FastQC (version 0.11.5)