FastQCFastQC Report
Mon 16 Jan 2017
ERR1146585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150208
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58290.14045079186392587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC8200.022.360623
TAACGCC9200.020.4401634
GTGATCG11250.020.0511138
TCTAACG8450.020.0299052
GTACTAG14000.019.8188131
CATGGGG103400.019.5505984
TTTAGGG29850.019.5303692
GGGCTAT29600.019.5284796
CTATTGA28750.019.2880659
GCTATTG28850.018.8956558
GTATAAG22250.018.5997161
ATTTAGG30700.018.382161
TACCTGG46100.017.7452222
GGCTATT33050.017.6319357
GTCCTAC15650.017.4287911
GTACATG281050.016.9838491
ACGCCTA10400.016.7195036
TACATGG278700.016.7005712
AGGGCTA33500.016.5585825
TGATCGC13650.016.5254429
Produced by FastQC (version 0.11.5)