##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146585_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4150208
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06375126258732	31.0	31.0	34.0	28.0	34.0
2	31.11677318341635	31.0	31.0	34.0	28.0	34.0
3	31.27253212369115	31.0	31.0	34.0	28.0	34.0
4	34.847162840994955	37.0	35.0	37.0	32.0	37.0
5	34.81303491294894	37.0	35.0	37.0	32.0	37.0
6	34.97429695089981	37.0	35.0	37.0	32.0	37.0
7	34.836751555584684	37.0	35.0	37.0	32.0	37.0
8	34.854155743519364	37.0	35.0	37.0	32.0	37.0
9	36.477361857526176	39.0	37.0	39.0	32.0	39.0
10-11	36.485667224389715	39.0	37.0	39.0	32.0	39.0
12-13	36.44683808618749	39.0	37.0	39.0	32.0	39.0
14-15	37.733288548429385	40.0	38.0	41.0	32.0	41.0
16-17	37.7301511153176	40.0	38.0	41.0	32.0	41.0
18-19	37.72066616902093	40.0	38.0	41.0	32.0	41.0
20-21	37.83643771589279	40.0	38.0	41.0	32.0	41.0
22-23	37.822209874782175	40.0	38.0	41.0	32.0	41.0
24-25	37.83064222323315	40.0	38.0	41.0	32.0	41.0
26-27	37.889607822065784	40.0	38.0	41.0	32.0	41.0
28-29	37.86803046979814	40.0	38.0	41.0	32.0	41.0
30-31	37.83486466220488	40.0	38.0	41.0	31.5	41.0
32-33	37.79772351168906	40.0	38.0	41.0	31.0	41.0
34-35	37.7461306758601	40.0	38.0	41.0	31.0	41.0
36-37	37.66256896039909	40.0	38.0	41.0	31.0	41.0
38-39	37.59480042927969	40.0	37.0	41.0	31.0	41.0
40-41	37.532789802342435	40.0	37.0	41.0	30.0	41.0
42-43	37.35793784311534	40.0	37.0	41.0	30.0	41.0
44-45	37.189509658311096	40.0	36.5	41.0	30.0	41.0
46-47	37.05218762529492	40.0	36.0	41.0	30.0	41.0
48-49	36.93177257621787	40.0	36.0	41.0	30.0	41.0
50-51	36.78958934106436	39.0	35.5	41.0	30.0	41.0
52-53	36.65912118139621	39.0	35.0	41.0	30.0	41.0
54-55	36.53691827012044	39.0	35.0	41.0	29.5	41.0
56-57	36.33311884609157	39.0	35.0	40.5	29.0	41.0
58-59	36.130967291277926	38.5	35.0	40.0	29.0	41.0
60-61	35.89893241495366	38.0	35.0	40.0	29.0	41.0
62-63	35.642295759634216	37.5	34.0	40.0	28.0	41.0
64-65	35.35414104546086	37.0	34.0	40.0	28.0	41.0
66-67	35.10682115209647	36.5	34.0	39.5	28.0	41.0
68-69	34.84740776847811	36.0	34.0	39.0	28.0	41.0
70-71	34.54723811433065	35.5	34.0	39.0	28.0	41.0
72-73	34.21197407455241	35.0	33.5	38.5	27.0	40.0
74-75	33.88750238542261	35.0	33.0	37.0	27.0	39.5
76-77	33.60537809189323	35.0	33.0	37.0	26.0	39.0
78-79	33.30823250304563	35.0	33.0	36.5	26.0	39.0
80-81	33.09072485041713	35.0	33.0	36.0	26.0	38.5
82-83	32.84841169406449	35.0	33.0	36.0	26.0	37.0
84-85	32.65518029457801	35.0	33.0	35.0	26.0	37.0
86-87	32.46435576240998	35.0	33.0	35.0	25.5	36.5
88-89	32.292966159768376	35.0	33.0	35.0	25.0	36.0
90-91	32.157124414005274	35.0	32.0	35.0	25.0	36.0
92-93	32.00537780275109	35.0	32.0	35.0	25.0	36.0
94-95	31.898460751846656	35.0	32.0	35.0	25.0	35.0
96-97	31.822712379716872	35.0	32.0	35.0	24.5	35.0
98-99	31.720784717296098	35.0	32.0	35.0	24.0	35.0
100	30.460068025506192	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1792463464289149
1101	2	0.19877251840239296
1101	3	0.17504913844301484
1101	4	0.19853535295148106
1101	5	0.1328920333554109
1101	6	0.21284010271690335
1101	7	0.21750187878323857
1101	8	0.2687598264836524
1101	9	0.3438488018894432
1101	10-11	0.3575901971721862
1101	12-13	0.39126776874873315
1101	14-15	0.5247920000021224
1101	16-17	0.5415263927700877
1101	18-19	0.5022921551653212
1101	20-21	0.4261241579720405
1101	22-23	0.4634591625184754
1101	24-25	0.5106001782872767
1101	26-27	0.5211125284875422
1101	28-29	0.4745717630825794
1101	30-31	0.5243588565835609
1101	32-33	0.5247322279000883
1101	34-35	0.4680789830809573
1101	36-37	0.4970617814202001
1101	38-39	0.48432614418763364
1101	40-41	0.4052129980107466
1101	42-43	0.499196631317929
1101	44-45	0.37515786517263905
1101	46-47	0.3605164029010268
1101	48-49	0.37607295902682836
1101	50-51	0.40253005917595175
1101	52-53	0.3864834995937301
1101	54-55	0.3842348738037771
1101	56-57	0.4009189320868458
1101	58-59	0.37761466712348835
1101	60-61	0.3764139236587525
1101	62-63	0.38648488972432915
1101	64-65	0.45335549796349284
1101	66-67	0.3274378550626835
1101	68-69	0.3854560701866845
1101	70-71	0.3116526777431332
1101	72-73	0.3337279761864451
1101	74-75	0.418960402873374
1101	76-77	0.38584718568109366
1101	78-79	0.3662688129960543
1101	80-81	0.33036687669005005
1101	82-83	0.2937449033199755
1101	84-85	0.25643185979034655
1101	86-87	0.30685979941331
1101	88-89	0.2497244103140872
1101	90-91	0.33587467875513966
1101	92-93	0.33526689948161703
1101	94-95	0.28924396152009635
1101	96-97	0.2928113261109573
1101	98-99	0.31609318218130156
1101	100	0.24968899383355492
1102	1	0.2986809422491241
1102	2	0.24892284308847934
1102	3	0.24079786724501417
1102	4	0.2583549607236364
1102	5	0.2811420024101352
1102	6	0.29059771406600277
1102	7	0.2931281242043653
1102	8	0.286781956928877
1102	9	0.3346419886809784
1102	10-11	0.4331975921843565
1102	12-13	0.42554770949789855
1102	14-15	0.4868091422189167
1102	16-17	0.44421302858546596
1102	18-19	0.4246567459060131
1102	20-21	0.4300898086852314
1102	22-23	0.45410301109610174
1102	24-25	0.4652150351312798
1102	26-27	0.3685823933903407
1102	28-29	0.3915485064963491
1102	30-31	0.46692825327345844
1102	32-33	0.44950164572587425
1102	34-35	0.4317901877835766
1102	36-37	0.3726518496834217
1102	38-39	0.3965492637486463
1102	40-41	0.3512944635528825
1102	42-43	0.33810570834004494
1102	44-45	0.24944276237474128
1102	46-47	0.23865511807929352
1102	48-49	0.28968697180226144
1102	50-51	0.3164841606765876
1102	52-53	0.22253742335355753
1102	54-55	0.25805126183491467
1102	56-57	0.2674322842645154
1102	58-59	0.28276497650786325
1102	60-61	0.22155524214686295
1102	62-63	0.252314222744765
1102	64-65	0.3689172886598797
1102	66-67	0.3536162384034185
1102	68-69	0.24860115706362507
1102	70-71	0.2503208537013748
1102	72-73	0.2881459455552786
1102	74-75	0.2750974351235911
1102	76-77	0.22710903140504968
1102	78-79	0.2136042344381366
1102	80-81	0.20140676877845465
1102	82-83	0.2464531503641325
1102	84-85	0.2012162446455008
1102	86-87	0.2152522489096782
1102	88-89	0.21103438546214193
1102	90-91	0.18853405473041818
1102	92-93	0.18320353176457616
1102	94-95	0.2415945065899301
1102	96-97	0.21362704158294576
1102	98-99	0.22615566721736613
1102	100	0.20987862308735572
1103	1	0.10959773301059172
1103	2	0.12995091458959607
1103	3	0.10612702393938633
1103	4	0.16366665597556818
1103	5	0.07068647287206886
1103	6	0.20256944508875563
1103	7	0.19028995820860217
1103	8	0.21434010764681233
1103	9	0.3603977413458139
1103	10-11	0.31166854161933344
1103	12-13	0.3398734585721428
1103	14-15	0.4035810502823409
1103	16-17	0.39209052284721224
1103	18-19	0.39655363485025674
1103	20-21	0.3816690737163384
1103	22-23	0.3909301586990708
1103	24-25	0.3911346592187641
1103	26-27	0.33545455279497816
1103	28-29	0.3306591047614802
1103	30-31	0.36599878351876214
1103	32-33	0.4150845823873155
1103	34-35	0.3490151716284231
1103	36-37	0.35777222945790754
1103	38-39	0.38616716556661856
1103	40-41	0.36622260862917955
1103	42-43	0.3288251117645302
1103	44-45	0.26382297058887616
1103	46-47	0.2849472942099993
1103	48-49	0.3674588007001347
1103	50-51	0.3223879654125881
1103	52-53	0.27182141291671513
1103	54-55	0.2011449311803304
1103	56-57	0.2789718814807358
1103	58-59	0.30123280934675023
1103	60-61	0.3112281658929419
1103	62-63	0.32097920029639226
1103	64-65	0.1888773669649808
1103	66-67	0.2190295006703593
1103	68-69	0.22915760578492694
1103	70-71	0.23180712235672019
1103	72-73	0.18614971073352393
1103	74-75	0.24207358528449419
1103	76-77	0.2119146249696513
1103	78-79	0.14328587890030065
1103	80-81	0.1338668674064678
1103	82-83	0.16581463461425017
1103	84-85	0.25337298666701
1103	86-87	0.2096569459151283
1103	88-89	0.21102444287029698
1103	90-91	0.23002896155958297
1103	92-93	0.1827802837586887
1103	94-95	0.24144239744103047
1103	96-97	0.31477837495955185
1103	98-99	0.26611131700451907
1103	100	0.1873371415311169
1104	1	0.05813952088919905
1104	2	0.07633128210204987
1104	3	0.08497768660952332
1104	4	0.15172624356520004
1104	5	0.1335347363031758
1104	6	0.1406211104895263
1104	7	0.15226384944028837
1104	8	0.17114551267874134
1104	9	0.22520795978831387
1104	10-11	0.24385846044581427
1104	12-13	0.21573205524252614
1104	14-15	0.3017977989217755
1104	16-17	0.3020038830480587
1104	18-19	0.24765623279577653
1104	20-21	0.28034975540523277
1104	22-23	0.2756936444891025
1104	24-25	0.26997088508431943
1104	26-27	0.28375403919601183
1104	28-29	0.302828828585227
1104	30-31	0.341078159934483
1104	32-33	0.3454687990615355
1104	34-35	0.2563883212893785
1104	36-37	0.3232862283542488
1104	38-39	0.3489577399182693
1104	40-41	0.30294226614093134
1104	42-43	0.3024540146512251
1104	44-45	0.2448501850364977
1104	46-47	0.2159636489247987
1104	48-49	0.23960489259471274
1104	50-51	0.24287353102535292
1104	52-53	0.20644579070053481
1104	54-55	0.16488274106138334
1104	56-57	0.2594583277447029
1104	58-59	0.21094265283013414
1104	60-61	0.264763778503351
1104	62-63	0.27318162694025716
1104	64-65	0.3139147671014939
1104	66-67	0.23791890281346184
1104	68-69	0.18445331211767524
1104	70-71	0.22004463025212573
1104	72-73	0.21490540505539002
1104	74-75	0.22766933847719173
1104	76-77	0.16226116641861665
1104	78-79	0.23436986077297206
1104	80-81	0.1997796011760613
1104	82-83	0.2000609198910155
1104	84-85	0.15492167514236854
1104	86-87	0.1538892650063346
1104	88-89	0.17576767307074448
1104	90-91	0.18856205553204575
1104	92-93	0.16499030334170328
1104	94-95	0.149693750348316
1104	96-97	0.11366085061879261
1104	98-99	0.15900023298408783
1104	100	0.16904667706196363
1105	1	0.06520593039061495
1105	2	-0.0029320824476606333
1105	3	0.014266725188861074
1105	4	0.02581474250412441
1105	5	-0.011871617343814478
1105	6	0.09419980850874055
1105	7	0.09971868906647785
1105	8	0.14903142046979667
1105	9	0.16426262084478793
1105	10-11	0.22430983364276358
1105	12-13	0.22121037030507296
1105	14-15	0.24400383970672834
1105	16-17	0.21024534203158396
1105	18-19	0.2520912578444623
1105	20-21	0.3039619018299291
1105	22-23	0.25675662569462787
1105	24-25	0.2727899738474946
1105	26-27	0.2283042988819659
1105	28-29	0.21290981508349205
1105	30-31	0.2734779316734546
1105	32-33	0.2605620620872173
1105	34-35	0.28601927684007933
1105	36-37	0.1706166908838469
1105	38-39	0.26006815868829136
1105	40-41	0.17945609459505363
1105	42-43	0.1956405180696592
1105	44-45	0.18654354552408137
1105	46-47	0.19278291774033107
1105	48-49	0.16250146846036273
1105	50-51	0.22175108873350524
1105	52-53	0.20900893946259913
1105	54-55	0.10861365296933201
1105	56-57	0.21899904201569598
1105	58-59	0.12003243869979485
1105	60-61	0.22084129278777453
1105	62-63	0.21627881742134036
1105	64-65	0.2522788857763274
1105	66-67	0.24347129431716752
1105	68-69	0.19529631437350048
1105	70-71	0.16936373634099056
1105	72-73	0.19485253615457765
1105	74-75	0.17518655900332192
1105	76-77	0.15705540099135362
1105	78-79	0.15541209664370115
1105	80-81	0.08095439063244925
1105	82-83	0.12479202493685904
1105	84-85	0.15034720217602882
1105	86-87	0.062095693527254525
1105	88-89	0.08012718114702011
1105	90-91	0.04142330159662322
1105	92-93	0.085739617151809
1105	94-95	0.06558127119741641
1105	96-97	0.05638476659937197
1105	98-99	0.08873116084177823
1105	100	0.2060529700774758
1106	1	-0.04171201580572159
1106	2	0.03407024734697117
1106	3	0.014265141881828924
1106	4	0.015324722310566585
1106	5	0.015441790061245797
1106	6	0.10949547109745339
1106	7	0.05497096015375291
1106	8	0.09693811913074768
1106	9	0.14606683976359847
1106	10-11	0.13653551389396057
1106	12-13	0.17211738023863177
1106	14-15	0.21943426172360603
1106	16-17	0.196326476017191
1106	18-19	0.16366399060850512
1106	20-21	0.19243365721577277
1106	22-23	0.23603698375979576
1106	24-25	0.2588173170599575
1106	26-27	0.21695169579464846
1106	28-29	0.22233386687285162
1106	30-31	0.22704450853976255
1106	32-33	0.21095799717695485
1106	34-35	0.19889187690078813
1106	36-37	0.21460441853214718
1106	38-39	0.16087590360407944
1106	40-41	0.19487346349542634
1106	42-43	0.241956707039634
1106	44-45	0.1878870787620599
1106	46-47	0.16044681599002786
1106	48-49	0.1856163761627272
1106	50-51	0.15698970615870422
1106	52-53	0.1518645692496321
1106	54-55	0.06961638409063653
1106	56-57	0.13236024507126842
1106	58-59	0.15402217964798837
1106	60-61	0.14192464534764326
1106	62-63	0.1589500474936898
1106	64-65	0.1804824256175337
1106	66-67	0.25728078714837466
1106	68-69	0.18687660701197473
1106	70-71	0.16925009933974167
1106	72-73	0.17938015427913712
1106	74-75	0.15005285150213155
1106	76-77	0.1723010366333
1106	78-79	0.13359820899660946
1106	80-81	0.1309595599910267
1106	82-83	0.19129532302096663
1106	84-85	0.23745604686799737
1106	86-87	0.12870492040732273
1106	88-89	0.13659565980950816
1106	90-91	0.08383356933717323
1106	92-93	0.12364597359621854
1106	94-95	0.1563549300127498
1106	96-97	0.11564528221636294
1106	98-99	0.138749010545272
1106	100	0.17737764085100238
1107	1	0.08955191080702818
1107	2	0.09908086396259108
1107	3	0.08027858505903751
1107	4	0.07182486633701046
1107	5	0.06976303472679746
1107	6	0.1438636468680201
1107	7	0.06590281684265165
1107	8	0.10304905088108285
1107	9	0.112505692036315
1107	10-11	0.09692889602402488
1107	12-13	0.18836783915844535
1107	14-15	0.16083021080497417
1107	16-17	0.17730340917918852
1107	18-19	0.20351040712338175
1107	20-21	0.20616319657739268
1107	22-23	0.22296567374853993
1107	24-25	0.23787553382249627
1107	26-27	0.22693695663849667
1107	28-29	0.1947333796235995
1107	30-31	0.22782078967087216
1107	32-33	0.24752767912898577
1107	34-35	0.28474621387478294
1107	36-37	0.24879814818550017
1107	38-39	0.24345785557841282
1107	40-41	0.20570787087999065
1107	42-43	0.20004704318559874
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2216	38-39	-0.3613102787189817
2216	40-41	-0.4583472877690369
2216	42-43	-0.39760238396542036
2216	44-45	-0.35177400455144436
2216	46-47	-0.2857927856334399
2216	48-49	-0.22887286083341962
2216	50-51	-0.30365301858363836
2216	52-53	-0.325588867916224
2216	54-55	-0.29105437406632007
2216	56-57	-0.2971207590913636
2216	58-59	-0.2689162123051503
2216	60-61	-0.286173272058619
2216	62-63	-0.35010296464470514
2216	64-65	-0.2952411717891934
2216	66-67	-0.2641635774899527
2216	68-69	-0.30395382074586763
2216	70-71	-0.36087815935343315
2216	72-73	-0.28894434922715817
2216	74-75	-0.2607360457310719
2216	76-77	-0.30505939195661114
2216	78-79	-0.23395582690142191
2216	80-81	-0.2991846680573076
2216	82-83	-0.23881036213156648
2216	84-85	-0.2539597428036373
2216	86-87	-0.3279308179140017
2216	88-89	-0.28172064501521277
2216	90-91	-0.31885080807840893
2216	92-93	-0.2867216795024632
2216	94-95	-0.3005189847763141
2216	96-97	-0.29744585447883054
2216	98-99	-0.316380527736559
2216	100	-0.2980747924254352
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1273.0
1	289.0
2	216.0
3	206.0
4	188.0
5	215.0
6	313.0
7	804.0
8	2155.0
9	3518.0
10	4397.0
11	5529.0
12	7204.0
13	9032.0
14	11234.0
15	12407.0
16	13266.0
17	14050.0
18	15792.0
19	17068.0
20	18800.0
21	20971.0
22	23119.0
23	26717.0
24	32452.0
25	38437.0
26	41363.0
27	41338.0
28	45269.0
29	55311.0
30	65200.0
31	76199.0
32	96890.0
33	130212.0
34	195855.0
35	302623.0
36	551590.0
37	1002760.0
38	1009529.0
39	256213.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93248456678465	19.66313190804611	13.07424150775206	24.330142017417174
2	15.004137316472885	20.963657194880142	39.74582024951201	24.286385239134965
3	18.820196540668178	26.543600467623264	29.558555152151182	25.077647839557375
4	12.624418480153329	17.403443349862982	38.55405445813395	31.418083711849743
5	13.512696872419728	38.2500395481098	34.517976062783106	13.719287516687373
6	30.144084032057044	37.215237128756804	18.782653195093435	13.858025644092715
7	26.673796817235836	32.547923534561136	22.904832988079708	17.873446660123328
8	25.803322048907113	34.67607394570839	20.877016474233358	18.643587531151145
9	26.66922902014815	16.094240274650858	20.535494905152333	36.70103580004866
10-11	24.720097111773548	26.209098489645704	28.0745912561786	20.996213142402148
12-13	25.742607440999617	24.060290605124337	27.536417265402985	22.66068468847306
14-15	23.511361160587956	25.188585053562196	25.351003610681115	25.94905017516873
16-17	22.719112313598394	27.88376304570095	26.40816405270367	22.98896058799699
18-19	22.663644392465613	27.973004500254707	27.271338531886098	22.092012575393582
20-21	23.768593674310896	27.133520972987075	26.37971929281407	22.718166059887963
22-23	23.577438869127075	26.96154339539657	25.783787876449665	23.677229859026692
24-25	23.462100720326543	27.178549231356296	26.101103729071855	23.2582463192453
26-27	23.49827875593972	26.937355010776916	26.223515543037614	23.340850690245755
28-29	23.014665969929204	27.300795018600432	26.530939508028435	23.153599503441928
30-31	23.514078131835113	27.24400294589593	26.1813124670528	23.06060645521616
32-33	23.11745471654296	27.477703424374923	26.040034600899553	23.364807258182566
34-35	23.438352532143682	27.35060275699362	26.347878753824318	22.86316595703838
36-37	23.737883464211453	26.97982398134764	26.29326248985315	22.989030064587762
38-39	23.71557763991932	26.855087159158753	26.478759857504723	22.9505753434172
40-41	23.299359797851714	26.863144521890998	26.695491478802573	23.142004201454714
42-43	23.438313576181542	26.85316601192688	26.215907125911414	23.49261328598016
44-45	23.303834488134022	27.168214560207478	26.255093627241383	23.27285732441712
46-47	23.295151044677354	27.002713250361072	26.375320828410732	23.32681487655085
48-49	23.575463713122453	26.70325796928641	26.56669176108426	23.154586556506874
50-51	23.163030891557646	26.993590792523687	26.322547939427317	23.520830376491347
52-53	22.922351522460055	27.222526379258365	26.109074464877903	23.746047633403677
54-55	22.985131678189248	27.033853904464355	26.15348719151443	23.827527225831968
56-57	23.51230647620736	26.667559030145604	26.132020353122698	23.688114140524334
58-59	23.245409447474316	26.594440976680872	26.636154635027797	23.523994940817015
60-61	23.27980161939756	26.939346334323123	26.435833635921718	23.345018410357603
62-63	23.16108135173429	26.71917432311202	26.330775563836646	23.788968761317044
64-65	22.627083538096873	26.984708912173478	26.48841636331514	23.899791186414514
66-67	23.33641906373569	26.531959017512445	26.245584261906636	23.886037656845232
68-69	23.174563282163987	26.529415389684136	26.244012552129192	24.05200877602269
70-71	23.07145999577425	26.523210738415614	26.294738172489613	24.110591093320522
72-73	23.434216432282817	26.403137488335478	26.44725103162741	23.7153950477543
74-75	23.24176528553816	26.879599572548713	26.262506272658303	23.616128869254826
76-77	23.08402617703434	26.519044388225304	26.474942457653345	23.921986977087016
78-79	23.11222098643667	26.5067196743894	26.58918846035191	23.79187087882202
80-81	22.916187790868054	26.652371746709775	26.686549308428976	23.74489115399319
82-83	23.115876125335415	26.204831155818532	26.830859375329773	23.84843334351628
84-85	23.169039206421452	26.56570378190531	26.269669962148868	23.99558704952437
86-87	22.877234653276453	26.578761842718713	26.611841018322462	23.932162485682372
88-89	23.297796674063907	25.966362456076624	26.56121680377006	24.174624066089407
90-91	23.34458204211157	25.981225031538635	26.63405453623889	24.04013839011091
92-93	23.232744685364175	26.293201219059988	26.54084312808776	23.933210967488076
94-95	22.62680385866669	26.227906948139623	26.906961283701143	24.238327909492543
96-97	22.9187681278346	26.010651838133636	26.756922024671642	24.313658009360122
98-99	23.019200556309247	26.238673888427723	26.461110753997534	24.281014801265492
100	23.22195540341892	26.045146321209582	26.137921006497848	24.594977268873645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1884.0
1	1223.5
2	547.5
3	557.5
4	554.0
5	501.0
6	466.5
7	451.0
8	469.0
9	490.0
10	450.5
11	422.0
12	440.0
13	486.0
14	526.5
15	580.0
16	654.0
17	766.0
18	939.5
19	1097.5
20	1382.0
21	1977.0
22	3016.0
23	4258.5
24	5768.0
25	8306.0
26	12037.5
27	16184.0
28	20897.5
29	27556.0
30	36173.5
31	44904.5
32	55799.0
33	65793.0
34	73034.0
35	82405.5
36	93157.0
37	104669.5
38	111787.0
39	112955.5
40	113100.5
41	113748.5
42	116273.0
43	123335.5
44	134763.5
45	149285.0
46	159685.0
47	170013.0
48	193177.0
49	198631.5
50	195953.0
51	190782.0
52	169110.5
53	157836.5
54	150598.5
55	138254.0
56	126624.5
57	112587.5
58	99134.5
59	89386.5
60	76284.0
61	61979.5
62	49856.0
63	39194.5
64	29511.0
65	21996.5
66	16905.0
67	12944.5
68	9777.5
69	7440.5
70	5995.0
71	4800.0
72	3784.5
73	3267.0
74	2614.0
75	1970.5
76	1428.0
77	1058.5
78	747.0
79	437.0
80	313.5
81	241.0
82	164.5
83	116.5
84	92.0
85	74.5
86	53.0
87	43.0
88	36.5
89	27.5
90	22.0
91	14.5
92	13.5
93	13.5
94	13.0
95	15.5
96	13.0
97	9.5
98	7.0
99	7.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09532052369423412
2	0.06245470106558514
3	0.07903218344719108
4	0.07989960985087977
5	0.08095979767761038
6	0.08674264036886825
7	0.08428493222508365
8	0.08409217080204173
9	0.07177953490523849
10-11	0.07792380526469998
12-13	0.06896039909325026
14-15	0.07726118787299335
16-17	0.07826113775502336
18-19	0.07759852036331673
20-21	0.0698037303190587
22-23	0.0732493407559332
24-25	0.07542995435409502
26-27	0.07028563387666352
28-29	0.0785743750674665
30-31	0.08245369870618534
32-33	0.08421264669144293
34-35	0.08562221459743705
36-37	0.08679083072462875
38-39	0.08877868289974865
40-41	0.08551378629697597
42-43	0.09100748685367094
44-45	0.08686311625826947
46-47	0.0848391213163292
48-49	0.09318810045183278
50-51	0.0903930598177248
52-53	0.0953687140499946
54-55	0.08771849507301803
56-57	0.09434466899008435
58-59	0.098404706462905
60-61	0.09726018551359354
62-63	0.09836856369608463
64-65	0.10036846346014464
66-67	0.1022117445679831
68-69	0.09971789365737814
70-71	0.101103366385492
72-73	0.0985251823523062
74-75	0.10212741144540226
76-77	0.09953717982327633
78-79	0.10594649713942049
80-81	0.1015129844094561
82-83	0.10574168812743842
84-85	0.10189850725553996
86-87	0.09866975341958764
88-89	0.09799508843894089
90-91	0.09885046725368946
92-93	0.09393505096612025
94-95	0.0928507679615094
96-97	0.09126048622141349
98-99	0.10391045460854009
100	0.24263844125402872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4150208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.5984291496551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27128520650086	41.62712500521156
2	14.39727720544894	16.5852110213199
3	5.710392578519195	9.867289270516624
4	2.7467253994837075	6.328282732628816
5	1.533187927391499	4.415460810448289
6	0.8997483221873761	3.109445399281837
7	0.6148120194890928	2.4788544581429215
8	0.4202704228292579	1.936553293842128
9	0.2911447587931222	1.5092532685475162
>10	1.0772131054600766	9.799768994078393
>50	0.029279254929254216	1.1468904612213477
>100	0.008380636722304765	0.7703859725295601
>500	7.079056130182767E-5	0.02075409390970287
>1k	1.4158112258845745E-4	0.16705210754209282
>5k	7.079056129422873E-5	0.23767311077931638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5829	0.14045079186392587	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4095177880241182E-5	0.0
10-11	0.0	0.0	0.0	2.4095177880241182E-5	0.0
12-13	0.0	0.0	0.0	2.4095177880241182E-5	0.0
14-15	0.0	0.0	0.0	7.228553364072355E-5	0.0
16-17	0.0	0.0	0.0	8.433312258084414E-5	0.0
18-19	0.0	0.0	0.0	1.0842830046108532E-4	0.0
20-21	0.0	0.0	0.0	1.8071383410180888E-4	0.0
22-23	0.0	0.0	0.0	3.493800792634972E-4	0.0
24-25	0.0	0.0	0.0	7.349029253473561E-4	0.0
26-27	0.0	0.0	0.0	0.0014698058506947122	0.0
28-29	0.0	0.0	0.0	0.004313036840563172	0.0
30-31	0.0	0.0	0.0	0.013481252023994942	0.0
32-33	0.0	0.0	0.0	0.03617890958718214	0.0
34-35	0.0	0.0	0.0	0.07392400573657995	0.0
36-37	0.0	0.0	0.0	0.13333066680031458	0.0
38-39	0.0	0.0	0.0	0.2192299759433744	0.0
40-41	0.0	0.0	0.0	0.3188153461224112	0.0
42-43	0.0	0.0	0.0	0.4165815303714898	0.0
44-45	0.0	0.0	0.0	0.5405030302095701	0.0
46-47	0.0	0.0	0.0	0.6937363139389641	0.0
48-49	0.0	0.0	0.0	0.8575112379909633	0.0
50-51	0.0	0.0	0.0	1.0334301316946042	0.0
52-53	0.0	0.0	0.0	1.208144266504233	0.0
54-55	0.0	0.0	0.0	1.403447730812528	0.0
56-57	0.0	0.0	0.0	1.6366529099264424	0.0
58-59	0.0	0.0	0.0	1.902675721313245	0.0
60-61	0.0	0.0	0.0	2.1839387327093003	0.0
62-63	0.0	0.0	0.0	2.4686232593643496	0.0
64-65	0.0	0.0	0.0	2.768523890850772	0.0
66-67	0.0	0.0	0.0	3.1074587105031846	0.0
68-69	0.0	0.0	0.0	3.48463257745154	0.0
70-71	0.0	0.0	0.0	3.9091173261677485	0.0
72-73	0.0	0.0	0.0	4.330397416225885	0.0
74-75	0.0	0.0	0.0	4.752063029130106	0.0
76-77	0.0	0.0	0.0	5.2210395238021805	0.0
78-79	0.0	0.0	0.0	5.739314752417228	0.0
80-81	0.0	0.0	0.0	6.2989854966305305	0.0
82-83	0.0	0.0	0.0	6.886980122442056	0.0
84-85	0.0	0.0	0.0	7.467011291964162	0.0
86-87	0.0	0.0	0.0	8.066908935648527	0.0
88	0.0	0.0	0.0	8.535210765339954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGC	820	0.0	22.36062	3
TAACGCC	920	0.0	20.440163	4
GTGATCG	1125	0.0	20.051113	8
TCTAACG	845	0.0	20.029905	2
GTACTAG	1400	0.0	19.818813	1
CATGGGG	10340	0.0	19.550598	4
TTTAGGG	2985	0.0	19.530369	2
GGGCTAT	2960	0.0	19.528479	6
CTATTGA	2875	0.0	19.288065	9
GCTATTG	2885	0.0	18.895655	8
GTATAAG	2225	0.0	18.599716	1
ATTTAGG	3070	0.0	18.38216	1
TACCTGG	4610	0.0	17.745222	2
GGCTATT	3305	0.0	17.631935	7
GTCCTAC	1565	0.0	17.428791	1
GTACATG	28105	0.0	16.983849	1
ACGCCTA	1040	0.0	16.719503	6
TACATGG	27870	0.0	16.700571	2
AGGGCTA	3350	0.0	16.558582	5
TGATCGC	1365	0.0	16.525442	9
>>END_MODULE