FastQCFastQC Report
Mon 16 Jan 2017
ERR1146585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150208
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112870.27196227273428225No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA107700.25950506577019755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107370.2587099249001496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53320.128475488457446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT129450.022.1465212-13
GTACTTT145550.019.59991514-15
AGTACTT139850.019.54181712-13
CTAGTAC19450.019.3440073
TACCTGG43900.019.1769832
CATGGGG100250.018.3898324
TACGGGC11100.018.2188724
GTACTAG14650.017.9784661
ACTTTTT169000.017.1860816-17
TAGTACT22000.017.101864
TACTTTT160000.016.9485914-15
ATACGGG11700.016.4806393
ATGGGGG65000.016.3425525
ACCTGGG49100.016.283343
GTACATG281700.016.111751
GGGCTAT26650.016.0493896
GTACCTG54700.015.9929231
TATTCGC3252.1615415E-715.9069549
GTATAAG22500.015.8866851
TACATGG282900.015.8435222
Produced by FastQC (version 0.11.5)