FastQCFastQC Report
Mon 16 Jan 2017
ERR1146584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7795072
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5788037.425242512192319No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1330701.7071041806926222No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1322641.6967643146849705No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1012851.2993465615198936No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA933261.197243591848799No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA894551.1475840120527432No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG880141.1290979736941493No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC724000.9287919341861115No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT429170.550565793362781No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA296200.37998366147227375No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA260770.3345318683393816No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT250720.32163910737450535No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG228580.293236547398151No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA140470.1802035952971313No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120540.15463615987126225No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC84770.10874819373060314No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78170.10028130593277393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA104050.027.6962033
GAGTACG107900.027.1956181
AGTACGC107650.026.944852
TACGCAG108950.026.4932144
CGCAGTG111550.025.825446
ACGCAGT123300.023.4478345
GTACTAG14200.022.5193751
CCGTATC8300.021.54966294
GTGATCG11350.021.5301768
TCTCGGG26300.019.68665594
TGATCGC12550.019.4707629
AGTAGGC49100.019.4365522
GTATAAG40750.018.57951
CATGGGG66250.018.3074264
GTAGGCA53950.017.9505443
GCATCGT13150.017.8683458
TCGCCGG4750.017.83667294
GCTTATG53950.017.5202541
ATACGGG8900.017.431143
TACCTGG50000.017.2062282
Produced by FastQC (version 0.11.5)