##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146584_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7795072
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.493347463628304	31.0	31.0	34.0	30.0	34.0
2	31.609947156357247	33.0	31.0	34.0	30.0	34.0
3	31.827894469736776	33.0	31.0	34.0	30.0	34.0
4	35.21192248128048	37.0	35.0	37.0	33.0	37.0
5	35.40114857181563	37.0	35.0	37.0	33.0	37.0
6	35.55349379710668	37.0	35.0	37.0	33.0	37.0
7	35.52199787250201	37.0	35.0	37.0	33.0	37.0
8	35.546806366894366	37.0	35.0	37.0	33.0	37.0
9	37.22292404739815	39.0	38.0	39.0	34.0	39.0
10-11	37.210340712696436	39.0	37.5	39.0	34.5	39.0
12-13	37.21340797108738	39.0	38.0	39.0	34.5	39.0
14-15	38.36195817049541	40.0	38.0	41.0	34.0	41.0
16-17	38.549348229240216	40.0	38.5	41.0	34.5	41.0
18-19	38.64560404316984	40.0	39.0	41.0	34.0	41.0
20-21	38.64507402882231	40.0	39.0	41.0	34.0	41.0
22-23	38.618552144226506	40.0	39.0	41.0	34.0	41.0
24-25	38.5237013846697	40.0	38.5	41.0	34.0	41.0
26-27	38.35475907085913	40.0	38.0	41.0	33.5	41.0
28-29	38.38032932858093	40.0	38.0	41.0	34.0	41.0
30-31	38.428098483246856	40.0	38.0	41.0	34.0	41.0
32-33	38.29180365492455	40.0	38.0	41.0	33.5	41.0
34-35	38.042916858240694	40.0	38.0	41.0	33.0	41.0
36-37	37.84991735804364	40.0	37.5	41.0	32.5	41.0
38-39	37.71785796205603	40.0	37.5	41.0	32.0	41.0
40-41	37.8918163937421	40.0	38.0	41.0	33.0	41.0
42-43	37.68621675335391	40.0	37.5	41.0	32.0	41.0
44-45	37.47821392797911	40.0	37.0	41.0	31.0	41.0
46-47	37.227908286158225	40.0	37.0	41.0	31.0	41.0
48-49	36.98246430565362	39.0	36.0	41.0	30.0	41.0
50-51	36.66064149247114	39.0	35.5	41.0	29.5	41.0
52-53	36.71255069869784	39.0	36.0	41.0	29.5	41.0
54-55	36.54926343207606	39.0	35.5	40.5	28.5	41.0
56-57	35.953547638815905	38.5	34.5	40.0	27.5	41.0
58-59	35.66842262650043	38.0	34.0	40.0	27.5	41.0
60-61	35.09552868786844	38.0	33.0	40.0	26.0	41.0
62-63	35.16842923323864	38.0	34.0	40.0	26.5	41.0
64-65	34.92795852302583	37.0	33.5	39.5	26.0	41.0
66-67	34.42703685097457	36.5	33.0	39.0	26.0	41.0
68-69	33.85892311193533	36.0	32.0	39.0	25.5	40.0
70-71	33.18703162716137	35.0	31.5	38.0	24.5	40.0
72-73	32.75403588318363	35.0	31.0	37.0	24.0	39.0
74-75	32.608369043929294	35.0	31.0	37.0	25.0	39.0
76-77	32.40292411667269	34.5	31.0	36.0	25.0	39.0
78-79	31.92887012461206	34.0	31.0	36.0	23.5	37.0
80-81	31.346922453057523	34.0	29.5	35.0	20.5	37.0
82-83	30.967595680963562	34.0	29.5	35.0	20.5	36.5
84-85	30.909996084705824	34.0	29.5	35.0	20.0	36.0
86-87	31.14380810081036	34.0	30.0	35.0	21.0	36.0
88-89	30.889115969679303	34.0	30.0	35.0	20.0	36.0
90-91	30.52986611284668	34.0	29.0	35.0	20.0	35.0
92-93	30.217185165191545	34.0	29.0	35.0	19.0	35.0
94-95	30.10074422660881	34.0	29.0	35.0	18.5	35.0
96-97	30.00803809124534	34.0	29.0	35.0	18.5	35.0
98-99	30.197939480225457	34.0	29.0	35.0	19.0	35.0
100	28.667864132621226	33.0	25.0	35.0	13.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17750181041781232
1101	2	0.24531785018200836
1101	3	0.22536239851224593
1101	4	0.2766007832518156
1101	5	0.21205227539279292
1101	6	0.2445849091913388
1101	7	0.2730196683742889
1101	8	0.2658328761295081
1101	9	0.39096135962186906
1101	10-11	0.3933614829355321
1101	12-13	0.39363744398936973
1101	14-15	0.49800655985631437
1101	16-17	0.46659514346985276
1101	18-19	0.44145764041312674
1101	20-21	0.4782994771402045
1101	22-23	0.5209556989037196
1101	24-25	0.5253432811298921
1101	26-27	0.5529536031542932
1101	28-29	0.5375031893846369
1101	30-31	0.5834778712037334
1101	32-33	0.5718589154843059
1101	34-35	0.5341147158730237
1101	36-37	0.5909668631103031
1101	38-39	0.6442836993318011
1101	40-41	0.57530336072832
1101	42-43	0.6000744635749271
1101	44-45	0.5417406211277651
1101	46-47	0.5312751942378711
1101	48-49	0.5905386387882459
1101	50-51	0.6541013431951939
1101	52-53	0.6447751832179591
1101	54-55	0.5745114246617931
1101	56-57	0.6718981567422802
1101	58-59	0.729271285978605
1101	60-61	0.7673566743346711
1101	62-63	0.7683292945395124
1101	64-65	0.7858993960549512
1101	66-67	0.7323664141325636
1101	68-69	0.706752517302327
1101	70-71	0.6923312839331999
1101	72-73	0.7514513080061178
1101	74-75	0.7155609928421782
1101	76-77	0.7446642958844762
1101	78-79	0.8127668262561976
1101	80-81	0.7938325366852617
1101	82-83	0.7466354612093191
1101	84-85	0.6904551056526458
1101	86-87	0.7445242986074874
1101	88-89	0.6829137350895849
1101	90-91	0.7290717745086148
1101	92-93	0.8034228426714876
1101	94-95	0.7950617200722156
1101	96-97	0.6986471988098302
1101	98-99	0.723334291851895
1101	100	0.8426638773334219
1102	1	0.2208228702604984
1102	2	0.2759462002175397
1102	3	0.2859791166323973
1102	4	0.32146226106166864
1102	5	0.27620123359069026
1102	6	0.3161469296917261
1102	7	0.2713102172766355
1102	8	0.30028263540423694
1102	9	0.3909150118280209
1102	10-11	0.4378751752564298
1102	12-13	0.45898266388539355
1102	14-15	0.5576803358284295
1102	16-17	0.5374568130441446
1102	18-19	0.4771616757940862
1102	20-21	0.5119275456190877
1102	22-23	0.5130261189414966
1102	24-25	0.5848300549070515
1102	26-27	0.5839517087369117
1102	28-29	0.5582414194526706
1102	30-31	0.5684279033957935
1102	32-33	0.5430411213022168
1102	34-35	0.5638624578255005
1102	36-37	0.6142876814770872
1102	38-39	0.5740021915421138
1102	40-41	0.5632419404478028
1102	42-43	0.6290995481764696
1102	44-45	0.5898942085815619
1102	46-47	0.631335159572977
1102	48-49	0.6977809011497556
1102	50-51	0.7096198925099557
1102	52-53	0.6778678620935352
1102	54-55	0.6181689220423863
1102	56-57	0.7169858323221732
1102	58-59	0.7586987005889014
1102	60-61	0.7938618764761998
1102	62-63	0.7161466887638639
1102	64-65	0.7919663010250488
1102	66-67	0.7438004396590472
1102	68-69	0.7242929620372038
1102	70-71	0.7723457753198062
1102	72-73	0.7921271268218604
1102	74-75	0.7265682221066214
1102	76-77	0.6677836624499918
1102	78-79	0.6611306889415296
1102	80-81	0.6496097626327746
1102	82-83	0.6972937556728205
1102	84-85	0.620687583920521
1102	86-87	0.5744850244288102
1102	88-89	0.5449809125521341
1102	90-91	0.6437490798528387
1102	92-93	0.6860168348643789
1102	94-95	0.7173635738706672
1102	96-97	0.607114855768458
1102	98-99	0.6508891847768048
1102	100	0.67943193980161
1103	1	0.14895865201423675
1103	2	0.16791342054395386
1103	3	0.20423260607138616
1103	4	0.20621022332985461
1103	5	0.21812980457037412
1103	6	0.260213783634903
1103	7	0.2367331919565956
1103	8	0.24782635944161768
1103	9	0.35334973213872445
1103	10-11	0.3038185021167834
1103	12-13	0.2974676723379801
1103	14-15	0.4365044215985563
1103	16-17	0.41036723454197954
1103	18-19	0.39680402793677416
1103	20-21	0.3764183797536944
1103	22-23	0.3888894447963338
1103	24-25	0.47562915371741354
1103	26-27	0.4392578241319214
1103	28-29	0.45583243395088857
1103	30-31	0.469359101274172
1103	32-33	0.5299136978624901
1103	34-35	0.5458300237964835
1103	36-37	0.5563065913769734
1103	38-39	0.5881343408857092
1103	40-41	0.5193089452176949
1103	42-43	0.5652417149279785
1103	44-45	0.5383656740516685
1103	46-47	0.6257765665348884
1103	48-49	0.6098724906748458
1103	50-51	0.6567574841843253
1103	52-53	0.6486121919515639
1103	54-55	0.5571846520122818
1103	56-57	0.5992860026306772
1103	58-59	0.6451049712097401
1103	60-61	0.7214674080461236
1103	62-63	0.7011221479997971
1103	64-65	0.6354427312079807
1103	66-67	0.6276135904920466
1103	68-69	0.6545937356525897
1103	70-71	0.6320038700012773
1103	72-73	0.6418086624892325
1103	74-75	0.6184325346195862
1103	76-77	0.536298113535004
1103	78-79	0.6011299348079326
1103	80-81	0.5946986247080197
1103	82-83	0.6413244740479165
1103	84-85	0.6205469694273305
1103	86-87	0.5277727326796615
1103	88-89	0.5079828041705667
1103	90-91	0.6392494160707329
1103	92-93	0.6213255268825044
1103	94-95	0.6828980974781906
1103	96-97	0.665517003792317
1103	98-99	0.6365142986823997
1103	100	0.6225357022998033
1104	1	0.14192062344608303
1104	2	0.1336597591780695
1104	3	0.17110910216120345
1104	4	0.19934705261432129
1104	5	0.14635143407829077
1104	6	0.15844967407468857
1104	7	0.166597541941492
1104	8	0.23816838062334256
1104	9	0.23625662820955995
1104	10-11	0.2669252700492919
1104	12-13	0.2886900366767762
1104	14-15	0.415980043515809
1104	16-17	0.40082836426375223
1104	18-19	0.37067705059885014
1104	20-21	0.3540773472287668
1104	22-23	0.3250750812059806
1104	24-25	0.3857886380831914
1104	26-27	0.41047948437125115
1104	28-29	0.4029197232243291
1104	30-31	0.40053833757520607
1104	32-33	0.40918023432910644
1104	34-35	0.45598561810992777
1104	36-37	0.520927148864267
1104	38-39	0.4646250089419013
1104	40-41	0.44714511916450306
1104	42-43	0.4644957293161127
1104	44-45	0.48290228031942917
1104	46-47	0.5543392563815956
1104	48-49	0.5397102910525291
1104	50-51	0.5919441794662106
1104	52-53	0.5062137869897256
1104	54-55	0.476006091607843
1104	56-57	0.6218527068169024
1104	58-59	0.6276613687022987
1104	60-61	0.657310987291055
1104	62-63	0.5811447161384464
1104	64-65	0.6388626707215579
1104	66-67	0.6216216699362533
1104	68-69	0.602657257873247
1104	70-71	0.6093096693496491
1104	72-73	0.6632219418442062
1104	74-75	0.6210402312048373
1104	76-77	0.5558598738537057
1104	78-79	0.5772778843905222
1104	80-81	0.5737005841273835
1104	82-83	0.5863479203658741
1104	84-85	0.5652751607727815
1104	86-87	0.5636013060477012
1104	88-89	0.5527461713142898
1104	90-91	0.5035056020805868
1104	92-93	0.5892853782358252
1104	94-95	0.5932184111953518
1104	96-97	0.5547594579174131
1104	98-99	0.5390933596198764
1104	100	0.5157580446598082
1105	1	0.07614229677210815
1105	2	0.04852216555430999
1105	3	0.0750897396003154
1105	4	0.12524800535196334
1105	5	0.10807685758758367
1105	6	0.13016668566103107
1105	7	0.09509042661526479
1105	8	0.18038572594866054
1105	9	0.20882975993707475
1105	10-11	0.22875473293558457
1105	12-13	0.2376279379589974
1105	14-15	0.34227630383576724
1105	16-17	0.2836279885585995
1105	18-19	0.27303252685694446
1105	20-21	0.2900232568447976
1105	22-23	0.29669399885631265
1105	24-25	0.3480962008616686
1105	26-27	0.3497570368997458
1105	28-29	0.31201779678931274
1105	30-31	0.3619022922891162
1105	32-33	0.38912087710205867
1105	34-35	0.3657097150733648
1105	36-37	0.40414277881890115
1105	38-39	0.37294244260082365
1105	40-41	0.341754830515562
1105	42-43	0.38354776764690257
1105	44-45	0.46730658264144154
1105	46-47	0.4830789761417762
1105	48-49	0.4762914050426872
1105	50-51	0.53436174248111
1105	52-53	0.48424827933129677
1105	54-55	0.43855718385427167
1105	56-57	0.5357497908362348
1105	58-59	0.5969220487934663
1105	60-61	0.6368860380060184
1105	62-63	0.5405617667007192
1105	64-65	0.597700478811042
1105	66-67	0.5348209764431928
1105	68-69	0.5337864608795186
1105	70-71	0.5655610938025575
1105	72-73	0.5507141543677605
1105	74-75	0.5688463144212008
1105	76-77	0.5048549751841165
1105	78-79	0.5410440542379114
1105	80-81	0.5472848029226007
1105	82-83	0.5555688979935418
1105	84-85	0.5275871621909047
1105	86-87	0.4765013080754592
1105	88-89	0.4551158395385926
1105	90-91	0.4751800961714494
1105	92-93	0.4938345937319646
1105	94-95	0.46299090848708246
1105	96-97	0.4824124702072119
1105	98-99	0.4544799810180251
1105	100	0.5098207333895992
1106	1	0.012114541140601176
1106	2	0.022936815656759535
1106	3	0.07250316097558951
1106	4	0.04108487600915112
1106	5	0.03620393018960755
1106	6	0.07916991621468128
1106	7	0.07098166842357756
1106	8	0.14254250426228765
1106	9	0.18868000719820088
1106	10-11	0.15894374223221774
1106	12-13	0.1877920559807933
1106	14-15	0.22166750517286005
1106	16-17	0.18699239327886374
1106	18-19	0.2455049924917816
1106	20-21	0.23470057130220567
1106	22-23	0.2737188276156175
1106	24-25	0.2603139556166951
1106	26-27	0.28840174135385865
1106	28-29	0.23631137834937022
1106	30-31	0.2674862252784962
1106	32-33	0.3045331435332983
1106	34-35	0.2547645593881427
1106	36-37	0.26312333566999513
1106	38-39	0.23930667919823634
1106	40-41	0.2334285609550406
1106	42-43	0.28709545965664773
1106	44-45	0.2797578221430683
1106	46-47	0.3269823333985187
1106	48-49	0.34565366133685416
1106	50-51	0.3497798345330807
1106	52-53	0.3039769871066653
1106	54-55	0.2857660144742624
1106	56-57	0.23498959566389743
1106	58-59	0.24994392453549352
1106	60-61	0.3107257815083315
1106	62-63	0.2920931255851258
1106	64-65	0.3279916728483627
1106	66-67	0.329205296312999
1106	68-69	0.20516148499498144
1106	70-71	0.24231711404237188
1106	72-73	0.2271730483151373
1106	74-75	0.254244794808713
1106	76-77	0.3095670165035216
1106	78-79	0.3150370892000858
1106	80-81	0.25560617575965594
1106	82-83	0.2861848586809188
1106	84-85	0.29834958309171355
1106	86-87	0.19237447973565835
1106	88-89	0.2805273406491082
1106	90-91	0.2119185055764099
1106	92-93	0.25404077312812845
1106	94-95	0.2767502165851923
1106	96-97	0.3035891022574262
1106	98-99	0.2741641545887781
1106	100	0.3820962191851187
1107	1	0.05750824528146126
1107	2	0.1018167771764169
1107	3	0.10841574131296738
1107	4	0.10081837673961047
1107	5	0.092503318125992
1107	6	0.12878930011406453
1107	7	0.09545971795127173
1107	8	0.13735717804189562
1107	9	0.1696957994558943
1107	10-11	0.1629712940421726
1107	12-13	0.18097816526466914
1107	14-15	0.2169325478297779
1107	16-17	0.19927965446776597
1107	18-19	0.20561472874826592
1107	20-21	0.2314265355833527
1107	22-23	0.2714617866651139
1107	24-25	0.30138927931037784
1107	26-27	0.27526888611395606
1107	28-29	0.24190166435364802
1107	30-31	0.26665461757183806
1107	32-33	0.2768674028475644
1107	34-35	0.25957629846909214
1107	36-37	0.26145405044091063
1107	38-39	0.30879083790960493
1107	40-41	0.22031671386286433
1107	42-43	0.2832488642291935
1107	44-45	0.2568868637062778
1107	46-47	0.2875668108467906
1107	48-49	0.28741499589465036
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2216	88-89	-1.9432830423226797
2216	90-91	-2.108805129499739
2216	92-93	-2.1169301686629147
2216	94-95	-2.1692148347055387
2216	96-97	-1.899767426780663
2216	98-99	-1.946992410257522
2216	100	-1.9503402074173621
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2445.0
1	647.0
2	457.0
3	416.0
4	355.0
5	481.0
6	589.0
7	1454.0
8	3583.0
9	4498.0
10	4492.0
11	5916.0
12	8103.0
13	10680.0
14	13924.0
15	15479.0
16	16682.0
17	19129.0
18	21415.0
19	24943.0
20	29011.0
21	34190.0
22	40753.0
23	48957.0
24	59575.0
25	72027.0
26	84169.0
27	94073.0
28	109383.0
29	135279.0
30	164960.0
31	204845.0
32	268833.0
33	368654.0
34	541197.0
35	813131.0
36	1201975.0
37	1633165.0
38	1382883.0
39	351711.0
40	613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.06348660875497	16.80799144423264	12.867027060901798	19.261494886110594
2	12.805179727113334	27.22759922842236	32.205830890166744	27.761390154297555
3	32.048032477029615	21.748018237899274	29.55219154853046	16.651757736540652
4	10.633381037576271	14.224642558433418	46.14440492915882	28.997571474831496
5	10.631947806920737	43.44772622130596	34.250238085791516	11.670087885981783
6	25.897594366073477	38.257570261324396	15.864397046770174	19.980438325831955
7	24.911402066155176	33.685127052005186	22.867036843386586	18.536434038453045
8	21.88426559566958	23.449864359800987	20.3120154732679	34.353854571261536
9	18.67283411716735	25.339886795127114	27.88150155140592	28.105777536299613
10-11	26.81597445463647	21.074854296351766	27.413438856467504	24.69573239254426
12-13	34.829678277267654	21.041834443888558	23.65915268635484	20.46933459248895
14-15	24.440086672401087	24.737378683065085	32.70847016458654	18.1140644799473
16-17	19.48698386742448	27.507486332139568	30.88084563358386	22.124684166852088
18-19	17.8138913667095	36.98013460156377	24.825530579052497	20.380443452674232
20-21	23.325305579103038	26.27290230468312	21.37836013766193	29.023431978551915
22-23	19.54932327492849	27.566293302438122	24.63962539554641	28.244758027086974
24-25	28.04888077319235	26.518994228532893	22.585730247385733	22.846394750889022
26-27	25.341497958971974	20.653571888915078	33.219239635536944	20.785690516576004
28-29	18.968080794956137	35.1080954613471	25.553942576386596	20.369881167310165
30-31	22.152789034981986	25.524700918598565	24.295614307372446	28.026895739047003
32-33	23.891493798656946	26.820813523656884	26.63762310333329	22.65006957435288
34-35	28.55170750736206	23.703444136816305	25.95521232804419	21.789636027777444
36-37	24.941491015589364	21.6322319740228	32.87438671740602	20.551890292981817
38-39	22.188943722440104	28.04755405594717	29.76462834036419	19.99887388124853
40-41	21.390852230818176	30.44686598925244	26.3211333245721	21.84114845535728
42-43	19.523204414781546	30.29734492638093	21.057080590103105	29.122370068734416
44-45	23.431322490561865	23.831222156115853	23.304286549733508	29.43316880358877
46-47	28.21347218956063	28.395889191076833	24.773370276403423	18.617268342959115
48-49	25.45318348020027	26.41793573707271	26.385473422346596	21.743407360380427
50-51	16.33758860421615	28.461428575555907	34.204840460245954	20.996142359981985
52-53	22.267374689543345	31.756635370915205	23.184936946849103	22.79105299269235
54-55	18.754102877916264	25.10306750333222	27.362267371436634	28.780562247314883
56-57	25.84800816284181	22.016411205467644	26.302256287139723	25.833324344550817
58-59	32.00632326403191	22.98026195187115	23.617487472097345	21.395927311999593
60-61	23.80194912909566	25.46507120671988	32.78630253422225	17.946677129962207
62-63	20.37152398145558	26.485121242170745	30.313136706637682	22.830218069735984
64-65	18.463203566050097	35.018841038855705	25.02001735972213	21.497938035372073
66-67	22.966722729264145	25.636409366248646	22.30156129614465	29.095306608342558
68-69	19.594274764263005	27.34377046662492	25.388419620838036	27.673535148274038
70-71	27.232766956939386	26.2622047456443	22.883190166085992	23.62183813133032
72-73	24.417983549227912	21.03557485082037	32.49092016389774	22.055521436053972
74-75	19.369987065383	34.100547127107525	25.334871994550817	21.194593812958658
76-77	22.259003848736768	24.581209655634982	24.855528704169156	28.304257791459094
78-79	23.68783960978667	25.787772699193862	27.346699612535012	23.177688078484458
80-81	27.58565734595945	23.8159417700975	26.192161554872673	22.406239329070377
82-83	23.974422775493586	21.892041663803848	32.141116518538546	21.99241904216402
84-85	21.680735551517557	27.57867431749968	29.168537605861765	21.57205252512099
86-87	21.51719699156665	29.523995605082	26.23226956729885	22.726537836052504
88-89	20.318961183786545	28.76415206218217	21.971598825952455	28.945287928078834
90-91	23.463440721373953	23.07765356603671	24.24096711068831	29.217938601901032
92-93	27.379357058933053	27.694888701983267	25.4342324064384	19.49152183264528
94-95	24.510411906887207	26.038097668058242	26.506445521200146	22.94504490385441
96-97	16.796375822310978	27.373132465515326	33.08268514067117	22.747806571502533
98-99	22.73025681278046	30.312991459094295	23.44611277447545	23.510638953649796
100	21.789858498209558	20.057751476002515	23.98723361350417	34.16515641228376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2862.0
1	1767.5
2	647.0
3	620.5
4	588.5
5	522.5
6	444.0
7	410.5
8	451.0
9	483.0
10	469.0
11	454.0
12	487.5
13	532.0
14	569.5
15	647.5
16	712.5
17	839.0
18	1057.0
19	1354.5
20	1763.0
21	2372.0
22	3594.0
23	5496.5
24	8171.5
25	11741.5
26	16763.5
27	22868.5
28	30032.0
29	38131.5
30	46191.5
31	54708.0
32	64219.5
33	73631.5
34	82918.5
35	92557.0
36	101534.0
37	111008.0
38	116587.0
39	117966.0
40	120731.5
41	122183.5
42	124567.0
43	130498.0
44	139588.5
45	159819.5
46	227149.5
47	614567.5
48	1465763.5
49	1499895.5
50	736614.0
51	347424.5
52	200363.5
53	148745.0
54	125853.5
55	106757.5
56	92321.5
57	78952.5
58	66082.0
59	57704.0
60	48291.5
61	38246.0
62	29997.5
63	23148.5
64	16999.5
65	12014.5
66	9080.5
67	7211.5
68	5654.0
69	4439.5
70	3738.0
71	2939.5
72	2287.0
73	1944.0
74	1542.5
75	1252.0
76	1016.5
77	722.0
78	523.0
79	376.0
80	270.0
81	220.5
82	192.5
83	146.0
84	93.0
85	58.5
86	45.5
87	37.5
88	31.0
89	26.5
90	24.5
91	18.0
92	13.0
93	15.5
94	20.5
95	19.5
96	14.5
97	13.0
98	15.0
99	15.0
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10319340219051215
2	0.0682225898619025
3	0.08563102431895434
4	0.08731157326064468
5	0.08868423537332304
6	0.09486762918931345
7	0.09082661455853133
8	0.0922249339069607
9	0.07707433619599666
10-11	0.0843417482224667
12-13	0.0734438373372305
14-15	0.08253291310202138
16-17	0.0839633039951395
18-19	0.08376446041806926
20-21	0.07498327148229035
22-23	0.07906277196669896
24-25	0.08113459375359201
26-27	0.07517570075042283
28-29	0.08359768838568778
30-31	0.08786961813822887
32-33	0.08981315374636693
34-35	0.09198119016732623
36-37	0.09192346138688648
38-39	0.09314859439399661
40-41	0.08949885260841722
42-43	0.09513703016469893
44-45	0.09132051634673805
46-47	0.08816467634936534
48-49	0.09724733780521848
50-51	0.09338592382469335
52-53	0.09946027438874203
54-55	0.09099980089985057
56-57	0.09734355243928472
58-59	0.10204524089065502
60-61	0.10071106463160313
62-63	0.10173093975270531
64-65	0.10366806105190562
66-67	0.10571422560304766
68-69	0.10268025747549221
70-71	0.10445060674231105
72-73	0.10133966690750258
74-75	0.10539992446509795
76-77	0.1022633273945385
78-79	0.10983862625002051
80-81	0.10448909259593753
82-83	0.10849162137309316
84-85	0.1052908812131562
86-87	0.10129476674493834
88-89	0.10036469194896468
90-91	0.10104460869636613
92-93	0.09521400187195192
94-95	0.09464312837649222
96-97	0.09318708024762311
98-99	0.10660581454539483
100	0.24810546971214634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7795072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.72905267426568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06320663614434	34.395085854130265
2	15.443762730277664	14.742323296845674
3	5.843256438493176	8.366792830262488
4	2.7459088811395365	5.242385185065715
5	1.4201662513377946	3.3891594908158016
6	0.8302531422164192	2.3776317574693246
7	0.5053598555384713	1.6884243015118494
8	0.31970956375568266	1.2207547687169185
9	0.2084597308981803	0.8954626928852247
>10	0.6144968129491797	4.095671809977752
>50	0.0037419327040699897	0.1150359674659113
>100	6.631930751274338E-4	0.05525211294370887
>500	1.6368338179069655E-4	0.051515253892642145
>1k	2.946281844832848E-4	0.2779808985676908
>5k	6.547292988402537E-5	0.2545905660850366
>10k+	4.910469741301903E-4	22.831933213363982
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	578803	7.425242512192319	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	133070	1.7071041806926222	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	132264	1.6967643146849705	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	101285	1.2993465615198936	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	93326	1.197243591848799	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	89455	1.1475840120527432	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	88014	1.1290979736941493	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	72400	0.9287919341861115	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	42917	0.550565793362781	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	29620	0.37998366147227375	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	26077	0.3345318683393816	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	25072	0.32163910737450535	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	22858	0.293236547398151	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	14047	0.1802035952971313	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	12054	0.15463615987126225	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	8477	0.10874819373060314	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7817	0.10028130593277393	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.1314471501995105E-5	0.0
3	0.0	0.0	0.0	5.1314471501995105E-5	0.0
4	0.0	0.0	0.0	5.1314471501995105E-5	0.0
5	0.0	0.0	0.0	7.697170725299265E-5	0.0
6	0.0	0.0	0.0	7.697170725299265E-5	0.0
7	0.0	0.0	0.0	7.697170725299265E-5	0.0
8	0.0	1.2828617875498776E-5	0.0	7.697170725299265E-5	0.0
9	0.0	1.2828617875498776E-5	0.0	1.1545756087948899E-4	0.0
10-11	0.0	1.2828617875498776E-5	0.0	3.5920130051396573E-4	0.0
12-13	0.0	1.2828617875498776E-5	0.0	5.195590239577005E-4	0.0
14-15	0.0	1.2828617875498776E-5	0.0	8.338601619074205E-4	0.0
16-17	0.0	1.2828617875498776E-5	0.0	0.0015458484539976026	0.0
18-19	0.0	1.2828617875498776E-5	0.0	0.002245008128212286	0.0
20-21	0.0	1.2828617875498776E-5	0.0	0.003585598696201908	0.0
22-23	0.0	1.2828617875498776E-5	0.0	0.005561205849028719	0.0
24-25	0.0	1.2828617875498776E-5	0.0	0.007293069262221055	0.0
26-27	0.0	1.2828617875498776E-5	0.0	0.009659949260250579	0.0
28-29	0.0	1.2828617875498776E-5	0.0	0.016715689091774906	0.0
30-31	0.0	1.2828617875498776E-5	0.0	0.031449356721785254	0.0
32-33	0.0	1.2828617875498776E-5	0.0	0.08298191472766384	0.0
34-35	0.0	1.2828617875498776E-5	0.0	0.1585553026322271	0.0
36-37	0.0	1.2828617875498776E-5	0.0	0.23655971362419745	0.0
38-39	1.2828617875498776E-5	1.2828617875498776E-5	0.0	0.3446536478431501	0.0
40-41	1.2828617875498776E-5	1.2828617875498776E-5	0.0	0.4599957511617596	0.0
42-43	1.2828617875498776E-5	1.2828617875498776E-5	0.0	0.5835802414653771	0.0
44-45	1.2828617875498776E-5	1.2828617875498776E-5	0.0	0.7389412182466051	0.0
46-47	1.2828617875498776E-5	1.2828617875498776E-5	0.0	0.943801417100445	0.0
48-49	1.2828617875498776E-5	1.2828617875498776E-5	0.0	1.1048518859094567	0.0
50-51	1.2828617875498776E-5	1.2828617875498776E-5	0.0	1.284381978768124	0.0
52-53	1.2828617875498776E-5	1.2828617875498776E-5	0.0	1.4635207987815892	0.0
54-55	1.2828617875498776E-5	1.2828617875498776E-5	0.0	1.6892788161546166	0.0
56-57	1.2828617875498776E-5	1.2828617875498776E-5	0.0	2.0426623384620437	0.0
58-59	1.2828617875498776E-5	1.2828617875498776E-5	0.0	2.342063036749372	0.0
60-61	1.2828617875498776E-5	1.2828617875498776E-5	0.0	2.6407325038177967	0.0
62-63	1.2828617875498776E-5	1.2828617875498776E-5	0.0	2.925944750734823	0.0
64-65	1.2828617875498776E-5	1.2828617875498776E-5	0.0	3.201227647416214	0.0
66-67	1.2828617875498776E-5	1.2828617875498776E-5	0.0	3.551429672490517	0.0
68-69	1.2828617875498776E-5	1.2828617875498776E-5	0.0	3.9608101118244963	0.0
70-71	1.2828617875498776E-5	1.2828617875498776E-5	0.0	4.357631334258362	0.0
72-73	1.2828617875498776E-5	1.2828617875498776E-5	0.0	4.700232403241433	0.0
74-75	1.2828617875498776E-5	1.2828617875498776E-5	0.0	5.104199165831951	0.0
76-77	1.2828617875498776E-5	1.2828617875498776E-5	0.0	5.470308677071873	0.0
78-79	1.2828617875498776E-5	1.2828617875498776E-5	0.0	6.110309179953694	0.0
80-81	1.2828617875498776E-5	1.2828617875498776E-5	0.0	6.756384033399563	0.0
82-83	1.2828617875498776E-5	1.2828617875498776E-5	0.0	7.237835647958095	0.0
84-85	1.2828617875498776E-5	1.2828617875498776E-5	0.0	7.767471551257	0.0
86-87	1.2828617875498776E-5	1.2828617875498776E-5	0.0	8.270924245472012	0.0
88	1.2828617875498776E-5	1.2828617875498776E-5	0.0	8.702434050641225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCA	10405	0.0	27.696203	3
GAGTACG	10790	0.0	27.195618	1
AGTACGC	10765	0.0	26.94485	2
TACGCAG	10895	0.0	26.493214	4
CGCAGTG	11155	0.0	25.82544	6
ACGCAGT	12330	0.0	23.447834	5
GTACTAG	1420	0.0	22.519375	1
CCGTATC	830	0.0	21.549662	94
GTGATCG	1135	0.0	21.530176	8
TCTCGGG	2630	0.0	19.686655	94
TGATCGC	1255	0.0	19.470762	9
AGTAGGC	4910	0.0	19.436552	2
GTATAAG	4075	0.0	18.5795	1
CATGGGG	6625	0.0	18.307426	4
GTAGGCA	5395	0.0	17.950544	3
GCATCGT	1315	0.0	17.868345	8
TCGCCGG	475	0.0	17.836672	94
GCTTATG	5395	0.0	17.520254	1
ATACGGG	890	0.0	17.43114	3
TACCTGG	5000	0.0	17.206228	2
>>END_MODULE