FastQCFastQC Report
Mon 16 Jan 2017
ERR1146584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7795072
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5887487.552823116964154No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1422231.8245245201070626No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1310021.6805745989260905No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1110611.4247591298707696No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA989461.269340424309102No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA953331.222990627924925No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG932621.196422560304767No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC788501.0115365194830785No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT465250.5968514466575806No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA273610.351003813691522No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT272570.34966963743247015No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC122920.15768937092563098No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT115870.14864519532340434No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC91850.11783085518645627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82380.10568215405835893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81960.10514335210758798No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81040.10396311926304208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG113200.026.75241
GTACGCA114800.026.3375683
TACGCAG114800.026.2554764
AGTACGC116750.026.0188312
CGCAGTG116250.025.9958746
ACGCAGT128850.023.5280975
AGTAGGC49300.019.9347842
GAGTACT119900.019.912112-13
CATGGGG68300.018.7951474
GTACTTT130100.018.15228514-15
GGGCTAT23350.017.9138776
GTAGGCA55650.017.913373
GCTTATG48050.017.61581
GTACCCG7750.017.5962281
AGTACTT126850.017.3206312-13
TAGGCAG56800.017.1366444
TACCTGG52450.016.9444892
GTACTAG13800.016.6970711
GAGTAGG69950.016.5375331
GGCTATT22900.016.2130287
Produced by FastQC (version 0.11.5)