##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7795072
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37050793116472	33.0	31.0	34.0	30.0	34.0
2	32.40256497951526	34.0	31.0	34.0	30.0	34.0
3	32.580620935893855	34.0	31.0	34.0	31.0	34.0
4	35.87540846319315	37.0	35.0	37.0	35.0	37.0
5	35.97806370486379	37.0	35.0	37.0	35.0	37.0
6	36.16195668237574	37.0	36.0	37.0	35.0	37.0
7	36.18042553038638	37.0	36.0	37.0	35.0	37.0
8	36.19573917983054	37.0	37.0	37.0	35.0	37.0
9	37.96604521420713	39.0	38.0	39.0	35.0	39.0
10-11	37.91683893362371	39.0	38.0	39.0	35.0	39.0
12-13	37.93106952700373	39.0	38.0	39.0	35.0	39.0
14-15	39.07983858263272	40.0	38.5	41.0	36.0	41.0
16-17	39.187719882510386	40.0	39.0	41.0	36.0	41.0
18-19	39.367982104591206	41.0	39.0	41.0	36.0	41.0
20-21	39.40327132321549	41.0	39.0	41.0	36.0	41.0
22-23	39.34335385228	41.0	39.0	41.0	36.0	41.0
24-25	39.26210636668911	40.5	39.0	41.0	36.0	41.0
26-27	39.053941836585985	40.0	38.5	41.0	35.5	41.0
28-29	39.08329826844447	40.0	38.5	41.0	36.0	41.0
30-31	39.12570692098803	40.0	39.0	41.0	36.0	41.0
32-33	39.02722431556758	40.0	39.0	41.0	35.0	41.0
34-35	38.83943304179871	40.0	38.0	41.0	35.0	41.0
36-37	38.69300065477266	40.0	38.0	41.0	34.5	41.0
38-39	38.613265919801634	40.0	38.0	41.0	35.0	41.0
40-41	38.77280524156801	40.0	38.0	41.0	35.0	41.0
42-43	38.71695200506166	40.0	38.0	41.0	35.0	41.0
44-45	38.599456746518825	40.0	38.0	41.0	35.0	41.0
46-47	38.41040102002906	40.0	38.0	41.0	34.0	41.0
48-49	38.10167949699502	40.0	38.0	41.0	33.0	41.0
50-51	37.88859948952364	40.0	37.0	41.0	33.0	41.0
52-53	38.03851215229314	40.0	38.0	41.0	33.0	41.0
54-55	37.94391302607596	40.0	37.5	41.0	33.0	41.0
56-57	37.6665291866451	40.0	37.0	41.0	32.5	41.0
58-59	37.47464718735119	39.0	36.5	41.0	32.0	41.0
60-61	36.935333310583914	39.0	36.0	41.0	31.0	41.0
62-63	36.93184809582259	39.0	35.5	41.0	31.0	41.0
64-65	36.66028222702754	38.5	35.0	40.0	31.0	41.0
66-67	36.19594430943037	37.5	35.0	39.0	31.0	41.0
68-69	35.63923501925319	37.0	35.0	39.0	30.0	41.0
70-71	34.97945041431305	36.0	34.0	38.0	29.5	40.0
72-73	34.48402907631899	35.5	34.0	37.0	29.0	39.5
74-75	34.264777862218594	35.0	34.0	37.0	29.0	39.0
76-77	34.01964330797714	35.0	34.0	36.5	29.0	39.0
78-79	33.70008160283831	35.0	34.0	36.0	29.0	37.5
80-81	33.31589265628335	35.0	33.0	36.0	29.0	37.0
82-83	32.97789231452897	35.0	33.0	35.0	27.5	37.0
84-85	32.826115461152895	35.0	33.0	35.0	27.5	36.0
86-87	32.943354596339844	35.0	33.0	35.0	29.0	36.0
88-89	32.749632780813315	35.0	33.0	35.0	27.5	36.0
90-91	32.5540679418997	35.0	33.0	35.0	27.0	35.0
92-93	32.44230438153747	35.0	33.0	35.0	26.5	35.0
94-95	32.30210554822329	35.0	33.0	35.0	25.5	35.0
96-97	32.18452311922199	35.0	32.0	35.0	25.0	35.0
98-99	32.28955274563211	35.0	33.0	35.0	25.0	35.0
100	31.30355819676842	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2985170186657484
1101	2	0.33633022826392533
1101	3	0.35129739460346343
1101	4	0.236679131224939
1101	5	0.22736360439603231
1101	6	0.23986348027187177
1101	7	0.22307830182852229
1101	8	0.22418760743115485
1101	9	0.2702752660007661
1101	10-11	0.31053751413264763
1101	12-13	0.30933193694603034
1101	14-15	0.46523307341408326
1101	16-17	0.47611167684261346
1101	18-19	0.43166089662362594
1101	20-21	0.3883805859579539
1101	22-23	0.41036834443980297
1101	24-25	0.4232874999542062
1101	26-27	0.4656345746867103
1101	28-29	0.4460180544454815
1101	30-31	0.49848132933297506
1101	32-33	0.43989985896342887
1101	34-35	0.45165085668432425
1101	36-37	0.4577528171169618
1101	38-39	0.4796533388673083
1101	40-41	0.4486973086874144
1101	42-43	0.46608736838481946
1101	44-45	0.5151471618898213
1101	46-47	0.5214212411629475
1101	48-49	0.6044081788172591
1101	50-51	0.5752211543477159
1101	52-53	0.5218915025526982
1101	54-55	0.5631275756103165
1101	56-57	0.5691221422926986
1101	58-59	0.5941383617155438
1101	60-61	0.6075701524529009
1101	62-63	0.5413378329711236
1101	64-65	0.4924132279109301
1101	66-67	0.49076120207233487
1101	68-69	0.4912109478683675
1101	70-71	0.46803048568033034
1101	72-73	0.48615659182135573
1101	74-75	0.4373656454867998
1101	76-77	0.39549371751395057
1101	78-79	0.37030445011225055
1101	80-81	0.40189452400720427
1101	82-83	0.47033817708289405
1101	84-85	0.4513968207236374
1101	86-87	0.41859762523959887
1101	88-89	0.5042755190896315
1101	90-91	0.5317025219514946
1101	92-93	0.5073373350198693
1101	94-95	0.5305788657235837
1101	96-97	0.4559743815486499
1101	98-99	0.5336460805908771
1101	100	0.5297782508356832
1102	1	0.23836137080644448
1102	2	0.23924795820350653
1102	3	0.27772761883189645
1102	4	0.17360017327913368
1102	5	0.17032076501333648
1102	6	0.20109742778959827
1102	7	0.2056130439081869
1102	8	0.19812598448083207
1102	9	0.24676454206309728
1102	10-11	0.2592299221303307
1102	12-13	0.2659146231097864
1102	14-15	0.4017555740110481
1102	16-17	0.4558861050687568
1102	18-19	0.39497459959495984
1102	20-21	0.3459262192380663
1102	22-23	0.35619030503715976
1102	24-25	0.3808905988335525
1102	26-27	0.38379662158656913
1102	28-29	0.3548913519695631
1102	30-31	0.3913962918595999
1102	32-33	0.39982983661735005
1102	34-35	0.4300535814105828
1102	36-37	0.42912850461513585
1102	38-39	0.4756374742547038
1102	40-41	0.4391394836360618
1102	42-43	0.44470478359845345
1102	44-45	0.44012302492185285
1102	46-47	0.49204065234131633
1102	48-49	0.5794781112294984
1102	50-51	0.5958992632897449
1102	52-53	0.5130162186945952
1102	54-55	0.523880058421625
1102	56-57	0.5722401556017758
1102	58-59	0.5526333812219875
1102	60-61	0.5346246556173
1102	62-63	0.5300585007305898
1102	64-65	0.512153794276017
1102	66-67	0.498251044505416
1102	68-69	0.47475594084786366
1102	70-71	0.507767168603074
1102	72-73	0.5026430048717643
1102	74-75	0.4331222730442832
1102	76-77	0.4219448885375314
1102	78-79	0.4747546562236167
1102	80-81	0.4937640127269489
1102	82-83	0.46901370655243824
1102	84-85	0.4923972334279014
1102	86-87	0.4822302782199017
1102	88-89	0.5174713272946008
1102	90-91	0.470495073050202
1102	92-93	0.486657243284526
1102	94-95	0.5185833733209435
1102	96-97	0.507434436194437
1102	98-99	0.5392192346173772
1102	100	0.5931252753568863
1103	1	0.22860162520084515
1103	2	0.2251409466464125
1103	3	0.21564514175441474
1103	4	0.18880853410691856
1103	5	0.1707182562622478
1103	6	0.18757172754513363
1103	7	0.1657822212112734
1103	8	0.17203767543688286
1103	9	0.24863259382180303
1103	10-11	0.25630686889478227
1103	12-13	0.22595339814544246
1103	14-15	0.39643893216794623
1103	16-17	0.44618763281772544
1103	18-19	0.35770547445277145
1103	20-21	0.3354932522879679
1103	22-23	0.3413158054362242
1103	24-25	0.34313757130139066
1103	26-27	0.3984087455409977
1103	28-29	0.39183054366403525
1103	30-31	0.4172567897134556
1103	32-33	0.3964071698752676
1103	34-35	0.4252012030617536
1103	36-37	0.4260821191146107
1103	38-39	0.43943277725929875
1103	40-41	0.41470005627483886
1103	42-43	0.4241585709202127
1103	44-45	0.466789503055125
1103	46-47	0.5148909002627349
1103	48-49	0.593475930526516
1103	50-51	0.5818385995264919
1103	52-53	0.4809694686961663
1103	54-55	0.48902494633851745
1103	56-57	0.5496947033415722
1103	58-59	0.5278875870878821
1103	60-61	0.5294124926993007
1103	62-63	0.5198007127301167
1103	64-65	0.4684211157820215
1103	66-67	0.47348325480814424
1103	68-69	0.44113650808201754
1103	70-71	0.4349194344603049
1103	72-73	0.45466282601641694
1103	74-75	0.4181385811832712
1103	76-77	0.39084473331169534
1103	78-79	0.4363676851383502
1103	80-81	0.4431624340739617
1103	82-83	0.42690791417799545
1103	84-85	0.45225611617897954
1103	86-87	0.43468843951650626
1103	88-89	0.47492562365653157
1103	90-91	0.4834842386642251
1103	92-93	0.48425815578617204
1103	94-95	0.5422977326530258
1103	96-97	0.46680863099970793
1103	98-99	0.48500488471195524
1103	100	0.48807917894090735
1104	1	0.22323398510692982
1104	2	0.2253664724307498
1104	3	0.2040588639918397
1104	4	0.19301737623478488
1104	5	0.13433172782470137
1104	6	0.1467379453970139
1104	7	0.13938456916906716
1104	8	0.11616320042375605
1104	9	0.15745526170138646
1104	10-11	0.18857461477867332
1104	12-13	0.16809149539247414
1104	14-15	0.3499395319167675
1104	16-17	0.3683548091784985
1104	18-19	0.3021972478673334
1104	20-21	0.23494847963332433
1104	22-23	0.2772917680248099
1104	24-25	0.2780101205441099
1104	26-27	0.3144287639448322
1104	28-29	0.30953575705383685
1104	30-31	0.3468442895359729
1104	32-33	0.2733506643365189
1104	34-35	0.31679317586095124
1104	36-37	0.33454255018298795
1104	38-39	0.38914952309825424
1104	40-41	0.3475033164888046
1104	42-43	0.3440961880958895
1104	44-45	0.3434864897622276
1104	46-47	0.41484867374460066
1104	48-49	0.48459745012100797
1104	50-51	0.46010947709701355
1104	52-53	0.4158539798498211
1104	54-55	0.4383233170484431
1104	56-57	0.44043237202153307
1104	58-59	0.40710318818169355
1104	60-61	0.4436727243635872
1104	62-63	0.4044653775758462
1104	64-65	0.3921611861802887
1104	66-67	0.40094056196997485
1104	68-69	0.34631205739143667
1104	70-71	0.42704954251234284
1104	72-73	0.39868613987809454
1104	74-75	0.35643690501807157
1104	76-77	0.3351020824472002
1104	78-79	0.3722335835585824
1104	80-81	0.4154916408397682
1104	82-83	0.4173826104572882
1104	84-85	0.42944565158808956
1104	86-87	0.38469715809630856
1104	88-89	0.4086788589644357
1104	90-91	0.4075203420454798
1104	92-93	0.4158154565488772
1104	94-95	0.4718368499495753
1104	96-97	0.49549718348984584
1104	98-99	0.4151944673851986
1104	100	0.47893687518578076
1105	1	0.1747478976257355
1105	2	0.18339021064931416
1105	3	0.17180665712357523
1105	4	0.13164318461618763
1105	5	0.08557647189133633
1105	6	0.10493338888579729
1105	7	0.1336236587825823
1105	8	0.11474979739536906
1105	9	0.14207694055936315
1105	10-11	0.1505896828856237
1105	12-13	0.18049786696155934
1105	14-15	0.27020212744446326
1105	16-17	0.3077154343520121
1105	18-19	0.2174822594596577
1105	20-21	0.19739015941266302
1105	22-23	0.2064730156226915
1105	24-25	0.22375693194219792
1105	26-27	0.26016694335689294
1105	28-29	0.20656697994017037
1105	30-31	0.25749163522480245
1105	32-33	0.2098138039573314
1105	34-35	0.25168697603451307
1105	36-37	0.24086544607857263
1105	38-39	0.2653909522019191
1105	40-41	0.24354218803896543
1105	42-43	0.27921797081802424
1105	44-45	0.27381803418361983
1105	46-47	0.2990819669619711
1105	48-49	0.37311751141721317
1105	50-51	0.41220798181743845
1105	52-53	0.32266836164028945
1105	54-55	0.3778333024457936
1105	56-57	0.36621855088263544
1105	58-59	0.39075241949807804
1105	60-61	0.38881609102222825
1105	62-63	0.37033214531267333
1105	64-65	0.3578975863986926
1105	66-67	0.33360998461135694
1105	68-69	0.32391218212189443
1105	70-71	0.3753985998434004
1105	72-73	0.34217279597238814
1105	74-75	0.347787888625291
1105	76-77	0.277213134033822
1105	78-79	0.33351431377928265
1105	80-81	0.35286247058598263
1105	82-83	0.29063820171820254
1105	84-85	0.3432297138254725
1105	86-87	0.3180381235536913
1105	88-89	0.3910436764564551
1105	90-91	0.32987775355045557
1105	92-93	0.3334220869233562
1105	94-95	0.35400952807616193
1105	96-97	0.320108146070055
1105	98-99	0.34167553864293154
1105	100	0.3510094404961599
1106	1	0.14430856108917567
1106	2	0.1302106836718977
1106	3	0.16339232055038622
1106	4	0.1070615950609124
1106	5	0.089379671820069
1106	6	0.11321854077115034
1106	7	0.12012788412478415
1106	8	0.11064365612629246
1106	9	0.11280157271113467
1106	10-11	0.14461579353825016
1106	12-13	0.13883888635335495
1106	14-15	0.24474957209567094
1106	16-17	0.2762931130534554
1106	18-19	0.20302508746125625
1106	20-21	0.2106008230263896
1106	22-23	0.20427190293064257
1106	24-25	0.21590806724270095
1106	26-27	0.2417718624463916
1106	28-29	0.21876689005009808
1106	30-31	0.22734046829648236
1106	32-33	0.21262713169070935
1106	34-35	0.20514558302060237
1106	36-37	0.23090518656913872
1106	38-39	0.2524607022818799
1106	40-41	0.23860249363529107
1106	42-43	0.2314432174693266
1106	44-45	0.2627120969547576
1106	46-47	0.2995376930222875
1106	48-49	0.328743734153754
1106	50-51	0.27828031056152014
1106	52-53	0.26645611725488294
1106	54-55	0.2728019145541083
1106	56-57	0.22367252549582872
1106	58-59	0.26713270608086503
1106	60-61	0.26328955164952816
1106	62-63	0.24811966593891555
1106	64-65	0.22083956912189961
1106	66-67	0.2445300849920713
1106	68-69	0.1769089074040906
1106	70-71	0.22573613392204095
1106	72-73	0.26126964290689614
1106	74-75	0.24858419448997893
1106	76-77	0.16283643172574358
1106	78-79	0.18351602506481157
1106	80-81	0.22660219299854845
1106	82-83	0.20491455895528787
1106	84-85	0.1950419327273849
1106	86-87	0.18091310861770182
1106	88-89	0.2475140168347707
1106	90-91	0.16998217398826654
1106	92-93	0.15534338494717304
1106	94-95	0.210870703866469
1106	96-97	0.19496653266129726
1106	98-99	0.18409797689191976
1106	100	0.24331542523623284
1107	1	0.08147003465147407
1107	2	0.07217230783434303
1107	3	0.10545086134663251
1107	4	0.09699260101119478
1107	5	0.06599779666215255
1107	6	0.08598657002078625
1107	7	0.06895014660566545
1107	8	0.09066875137873609
1107	9	0.10291722131174907
1107	10-11	0.11248432190139823
1107	12-13	0.12074953265273791
1107	14-15	0.24790103761975502
1107	16-17	0.2585912469429985
1107	18-19	0.17500354677308394
1107	20-21	0.15270660531326286
1107	22-23	0.14677311796747006
1107	24-25	0.17926441052227204
1107	26-27	0.2106341943006811
1107	28-29	0.20309008053995825
1107	30-31	0.21824227256353623
1107	32-33	0.16849705868828835
1107	34-35	0.1632831803040773
1107	36-37	0.1767031418715277
1107	38-39	0.21115763881437033
1107	40-41	0.1786927678291974
1107	42-43	0.19679626529703853
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2216	70-71	-1.2148079094179636
2216	72-73	-1.188071086262859
2216	74-75	-1.183003151382593
2216	76-77	-1.141087408442921
2216	78-79	-1.1836164039436596
2216	80-81	-1.3448205256490198
2216	82-83	-1.2940095100439066
2216	84-85	-1.382859544159981
2216	86-87	-1.3348839622005677
2216	88-89	-1.2542855027216895
2216	90-91	-1.4208387542992682
2216	92-93	-1.4269613195068729
2216	94-95	-1.3929406070005719
2216	96-97	-1.3566320246501924
2216	98-99	-1.4081103622459992
2216	100	-1.6101423927464253
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	34.0
8	184.0
9	302.0
10	336.0
11	224.0
12	229.0
13	297.0
14	550.0
15	975.0
16	1797.0
17	3389.0
18	6056.0
19	9063.0
20	13162.0
21	19273.0
22	28047.0
23	34125.0
24	37714.0
25	40935.0
26	46763.0
27	58018.0
28	73144.0
29	89547.0
30	111549.0
31	139373.0
32	177623.0
33	231834.0
34	328459.0
35	515297.0
36	1016594.0
37	2096181.0
38	2241585.0
39	471182.0
40	1230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.71093257686632	16.98062254580984	13.005976267352523	19.302468609971324
2	12.930521649226101	27.455653215192417	31.75719736812902	27.856627767452462
3	32.16924977732689	21.671805178126295	29.70949931232079	16.449445732226017
4	10.70816920650645	14.53772742494298	45.467160280411555	29.28694308813901
5	10.58566920482912	43.39244308891044	34.316675642646366	11.705212063614082
6	26.214006008465528	38.201365574343185	15.547716009493154	20.036912407698132
7	24.678585310972192	33.79137069968582	22.794676635360556	18.735367353981427
8	22.40934973914826	23.346355715127093	20.19351790452417	34.05077664120048
9	18.770769532340434	24.909429957798977	27.782604188902937	28.53719632095765
10-11	26.85921845660516	21.038861392140127	27.430201654560072	24.671718496694638
12-13	35.017835627432305	20.907850498366148	23.75958169469121	20.314732179510337
14-15	24.511835623263885	24.640962126185155	32.88818867414772	17.959013576403237
16-17	19.476314761492716	27.24103581185227	31.1585564932595	22.12409293339551
18-19	17.722631911681546	36.39775760660849	25.631085261134483	20.248525220575484
20-21	23.103873589626964	25.76843413514706	22.450145165721377	28.67754710950459
22-23	19.42166794610749	27.097555994351303	25.51537048022135	27.965405579319857
24-25	27.693118164912395	26.226492840604937	23.51433059245636	22.566058402026307
26-27	25.22458622017862	20.19724036673238	34.004187910095595	20.573985502993402
28-29	18.91616388637359	34.57500678473571	26.515067886163713	19.993761442726985
30-31	22.11688908220229	25.055597736847705	25.22869462203385	27.598818558916154
32-33	23.593139596759585	26.387912486023186	27.6198029211351	22.39914499608213
34-35	28.320501271446602	23.33118624646539	26.889799921950548	21.45851256013746
36-37	24.757320848854906	21.22898638702995	33.63505196748815	20.378640796626996
38-39	22.01628543416118	27.521395920014136	30.615577095709718	19.84674155011496
40-41	21.357905994350375	29.861874195120315	27.06163535067177	21.71858445985754
42-43	19.564533849077982	29.893604574787762	21.891138401287378	28.650723174846878
44-45	23.363845086400577	23.363428154019697	24.094297789014	29.17842897056573
46-47	28.083287748977813	28.113685159033807	25.34605838150052	18.456968710487857
48-49	25.582761448960618	26.15669519561964	26.758390101632912	21.502153253786833
50-51	16.3888672227787	28.207866457166787	34.48460771112826	20.918658608926254
52-53	22.377013643865784	31.44488069755626	23.524114656734028	22.653991001843927
54-55	18.945303880728133	24.92177068676562	27.633478605347612	28.499446827158632
56-57	25.748984260153584	21.91014126003184	26.712222886888448	25.628651592926133
58-59	31.812753804538406	22.785511403817278	23.985357535943397	21.416377255700915
60-61	23.660436922379834	25.405422152057795	33.077317411379035	17.856823514183347
62-63	20.33368862283976	26.27923625648913	30.567649232336695	22.819425888334415
64-65	18.37661225149818	34.80035409703236	25.36522430261249	21.45780934885697
66-67	22.862120424414584	25.449166113158196	22.788502461041396	28.900211001385827
68-69	19.556184545421367	27.21627574781155	25.651013812111994	27.576525894655084
70-71	26.921159464317878	26.239792517343673	23.174552122884414	23.66449589545403
72-73	24.302502107652803	20.797977269101608	32.74528168331525	22.15423893993034
74-75	19.234659874134604	33.83449425164227	25.539777301251405	21.391068572971715
76-77	22.137176690329927	24.435933405377888	25.014184292982673	28.412705611309512
78-79	23.311136081665087	25.727652961356178	27.594224677769315	23.366986279209424
80-81	27.20440416752806	23.705684703124017	26.44625602334733	22.643655106000583
82-83	23.556158134745296	21.872452897914634	32.30140613665626	22.26998283068381
84-85	21.244040186418985	27.39765374604804	29.339944732673285	22.018361334859694
86-87	21.169419602538632	29.245483556790752	26.314388115978915	23.2707087246917
88-89	19.84075964917322	28.71784250357149	22.165953053416313	29.27544479383898
90-91	22.89743242219554	22.814931564762627	24.549412349766435	29.7382236632754
92-93	26.628929545236023	27.78398077604351	25.52283877537655	20.06425090334392
94-95	23.945274855985787	25.977195312315587	26.598485913649974	23.479043918048653
96-97	16.021729967698057	27.193479341963734	33.35353634743317	23.43125434290504
98-99	21.95263066170019	30.03172456516871	23.591911727210434	24.423733045920663
100	20.892608028380472	19.88389293746657	24.39261277865836	34.8308862554946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	136.5
2	192.0
3	414.0
4	313.5
5	167.5
6	324.5
7	699.0
8	1131.5
9	1435.5
10	1271.0
11	1008.5
12	1117.5
13	1363.5
14	1623.0
15	1898.0
16	2242.5
17	2640.5
18	3122.5
19	3785.5
20	4526.5
21	5269.0
22	6440.5
23	8269.5
24	10925.0
25	14355.0
26	19105.0
27	24654.0
28	31240.0
29	38891.0
30	46512.0
31	54453.5
32	63467.0
33	72648.5
34	81144.5
35	90113.5
36	99390.0
37	108266.5
38	113212.0
39	115162.5
40	118178.0
41	119627.5
42	121817.0
43	126999.0
44	135533.5
45	153079.5
46	203544.5
47	614526.0
48	1530565.5
49	1534638.5
50	715175.0
51	335697.5
52	198965.5
53	143381.0
54	117018.0
55	101067.5
56	88841.5
57	77602.0
58	65564.5
59	57168.5
60	48160.5
61	37748.0
62	28936.0
63	22033.5
64	16024.0
65	11196.5
66	8325.0
67	6603.5
68	5200.5
69	4119.5
70	3380.0
71	2658.0
72	2053.5
73	1686.0
74	1332.0
75	1052.0
76	820.0
77	587.0
78	405.5
79	250.0
80	167.0
81	117.5
82	80.5
83	44.5
84	18.5
85	15.0
86	9.0
87	4.0
88	2.0
89	2.0
90	2.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0030788682901197063
2	0.004015357395031117
3	1.6677203238148409E-4
4	0.052533190200167494
5	0.0024887518678467623
6	0.005169933003826007
7	2.5657235750997552E-5
8	3.976871541404621E-4
9	0.0
10-11	1.15457560879489E-4
12-13	0.0
14-15	3.848585362649633E-5
16-17	3.46372682638467E-4
18-19	1.0262894300399021E-4
20-21	1.9884357707023104E-4
22-23	0.0
24-25	0.0
26-27	1.8601495919473227E-4
28-29	6.414308937749388E-6
30-31	1.9884357707023104E-4
32-33	2.5657235750997556E-4
34-35	1.796006502569829E-4
36-37	7.697170725299265E-5
38-39	8.915889423471649E-4
40-41	5.195590239577005E-4
42-43	0.0
44-45	5.516305686464473E-4
46-47	0.0
48-49	1.0904325194173961E-4
50-51	0.0
52-53	3.9127284520271274E-4
54-55	5.131447150199511E-4
56-57	1.9242926813248164E-4
58-59	9.300747959736612E-4
60-61	6.2860227589944E-4
62-63	2.629866664477249E-4
64-65	4.29758698829209E-4
66-67	0.0012764474786121284
68-69	0.0027004240627924924
70-71	0.003848585362649633
72-73	0.0033803408101939275
74-75	0.00304038243649321
76-77	0.004599059508366312
78-79	0.0014496338199313618
80-81	0.001173818535608138
82-83	7.889599993431747E-4
84-85	6.478452027126883E-4
86-87	0.0
88-89	0.0
90-91	1.9242926813248163E-5
92-93	8.338601619074204E-5
94-95	1.0262894300399021E-4
96-97	3.9127284520271274E-4
98-99	1.0904325194173961E-4
100	0.0776644526182696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7795072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.06823314996129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75995128021175	32.59785933256697
2	15.9823266198902	14.725550980078689
3	6.1941570964827575	8.560616198647647
4	2.8443933180666203	5.241446981875402
5	1.5453414676600044	3.5595575514232216
6	0.8863717837237094	2.450014919407854
7	0.5560182386577495	1.7930344497881212
8	0.3225577735979817	1.18877333747554
9	0.2321158771004478	0.9623851509664071
>10	0.6671840624199386	4.324229177993644
>50	0.005511963174060098	0.17338532172315424
>100	0.0023718740281409296	0.21294139513700017
>500	6.45486945091869E-4	0.20967298450816446
>1k	4.416469205960781E-4	0.46462194971466475
>5k	1.6986420022665965E-4	0.6163084096636743
>10k+	4.4164692058931503E-4	22.91960185902991
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	588748	7.552823116964154	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	142223	1.8245245201070626	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	131002	1.6805745989260905	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	111061	1.4247591298707696	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	98946	1.269340424309102	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	95333	1.222990627924925	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	93262	1.196422560304767	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	78850	1.0115365194830785	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	46525	0.5968514466575806	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	27361	0.351003813691522	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	27257	0.34966963743247015	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	12292	0.15768937092563098	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	11587	0.14864519532340434	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	9185	0.11783085518645627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8238	0.10568215405835893	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8196	0.10514335210758798	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8104	0.10396311926304208	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.848585362649633E-5	0.0
3	0.0	0.0	0.0	3.848585362649633E-5	0.0
4	0.0	0.0	0.0	5.1314471501995105E-5	0.0
5	0.0	0.0	0.0	8.980032512849143E-5	0.0
6	0.0	0.0	0.0	8.980032512849143E-5	0.0
7	0.0	0.0	0.0	8.980032512849143E-5	0.0
8	0.0	0.0	0.0	8.980032512849143E-5	0.0
9	0.0	0.0	0.0	1.2828617875498778E-4	0.0
10-11	0.0	0.0	0.0	2.758152843232237E-4	0.0
12-13	0.0	0.0	0.0	4.5541593458020655E-4	0.0
14-15	0.0	0.0	0.0	7.825456904054254E-4	0.0
16-17	0.0	0.0	0.0	0.0014945339824956076	0.0
18-19	0.0	0.0	0.0	0.0022001079656480405	0.0
20-21	0.0	0.0	0.0	0.003540698533637662	0.0
22-23	0.0	0.0	0.0	0.005541962922215472	0.0
24-25	0.0	0.0	0.0	0.007280240644345556	0.0
26-27	0.0	0.0	0.0	0.009640706333437332	0.0
28-29	0.0	0.0	0.0	0.016702860473899406	0.0
30-31	0.0	0.0	0.0	0.031481428266474	0.0
32-33	0.0	0.0	0.0	0.08318717261367181	0.0
34-35	0.0	0.0	0.0	0.15889526100592785	0.0
36-37	0.0	0.0	0.0	0.23695098646940016	0.0
38-39	0.0	0.0	0.0	0.34490380589172237	0.0
40-41	0.0	0.0	0.0	0.46022025197458083	0.0
42-43	0.0	0.0	0.0	0.5836187273190035	0.0
44-45	0.0	0.0	0.0	0.7380303863774447	0.0
46-47	0.0	0.0	0.0	0.9425762840933349	0.0
48-49	0.0	0.0	0.0	1.1036780673738487	0.0
50-51	0.0	0.0	0.0	1.28388166267098	0.0
52-53	0.0	0.0	0.0	1.4633155408955814	0.0
54-55	0.0	0.0	0.0	1.689888175503703	0.0
56-57	0.0	0.0	0.0	2.0448111319561897	0.0
58-59	0.0	0.0	0.0	2.345693535608138	0.0
60-61	0.0	0.0	0.0	2.6462680524310747	0.0
62-63	0.0	0.0	0.0	2.933038976419974	0.0
64-65	0.0	0.0	0.0	3.2097779212302338	0.0
66-67	0.0	0.0	0.0	3.562173639961247	0.0
68-69	0.0	0.0	0.0	3.9747035049836614	0.0
70-71	0.0	0.0	0.0	4.374693396032775	0.0
72-73	0.0	0.0	0.0	4.720315604525526	0.0
74-75	0.0	0.0	0.0	5.127066177195028	0.0
76-77	0.0	0.0	0.0	5.495125638352025	0.0
78-79	0.0	0.0	0.0	6.1368836618827896	0.0
80-81	0.0	0.0	0.0	6.784530021018408	0.0
82-83	0.0	0.0	0.0	7.266860395901411	0.0
84-85	0.0	0.0	0.0	7.797080001313651	0.0
86-87	0.0	0.0	0.0	8.301482013251448	0.0
88	0.0	0.0	0.0	8.733440820046306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	11320	0.0	26.7524	1
GTACGCA	11480	0.0	26.337568	3
TACGCAG	11480	0.0	26.255476	4
AGTACGC	11675	0.0	26.018831	2
CGCAGTG	11625	0.0	25.995874	6
ACGCAGT	12885	0.0	23.528097	5
AGTAGGC	4930	0.0	19.934784	2
GAGTACT	11990	0.0	19.9121	12-13
CATGGGG	6830	0.0	18.795147	4
GTACTTT	13010	0.0	18.152285	14-15
GGGCTAT	2335	0.0	17.913877	6
GTAGGCA	5565	0.0	17.91337	3
GCTTATG	4805	0.0	17.6158	1
GTACCCG	775	0.0	17.596228	1
AGTACTT	12685	0.0	17.32063	12-13
TAGGCAG	5680	0.0	17.136644	4
TACCTGG	5245	0.0	16.944489	2
GTACTAG	1380	0.0	16.697071	1
GAGTAGG	6995	0.0	16.537533	1
GGCTATT	2290	0.0	16.213028	7
>>END_MODULE