FastQCFastQC Report
Mon 16 Jan 2017
ERR1146583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109966
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA161470.39287429628371623No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG73830.17963652254057577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43600.019.6233832
GTACTAG16900.019.4767591
CATGGGG79350.019.0762334
CTAACGC6550.018.6604023
TAACGCC7650.017.2060154
ATGGGGG49050.016.771515
GTATAAG24050.016.6191651
TCTAACG7200.016.3228682
GTACCTG54050.016.268641
GTACCCG4951.8189894E-1216.1491281
ACCTGGG48900.015.9583583
TAGGCAT22950.015.5669665
CAGTACG4852.910383E-1115.5031199
GTATATA26100.015.4939911
TACGGGC9450.015.4210374
CGGGCGT9500.015.3352026
CTAGTAC20950.015.2585723
TAGTACT23350.015.0993434
GGCGTCG10000.015.0383928
TGGGGGG46350.014.7017726
Produced by FastQC (version 0.11.5)