##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146583_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4109966
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.020293111913823	31.0	31.0	34.0	28.0	34.0
2	31.096455299143592	31.0	31.0	34.0	27.0	34.0
3	31.274451175508506	31.0	31.0	34.0	28.0	34.0
4	34.770980343876325	37.0	35.0	37.0	32.0	37.0
5	34.845463685101045	37.0	35.0	37.0	32.0	37.0
6	34.80006014648296	37.0	35.0	37.0	32.0	37.0
7	34.7794227494826	37.0	35.0	37.0	32.0	37.0
8	34.80060710964519	37.0	35.0	37.0	32.0	37.0
9	36.39547602096952	39.0	37.0	39.0	32.0	39.0
10-11	36.403679616814344	39.0	37.0	39.0	32.0	39.0
12-13	36.35862960910139	39.0	37.0	39.0	32.0	39.0
14-15	37.63660441473239	40.0	38.0	41.0	32.0	41.0
16-17	37.628739386165236	40.0	38.0	41.0	32.0	41.0
18-19	37.629688299124616	40.0	38.0	41.0	31.0	41.0
20-21	37.733875049088	40.0	38.0	41.0	32.0	41.0
22-23	37.716971137960755	40.0	38.0	41.0	31.0	41.0
24-25	37.731560674711176	40.0	38.0	41.0	31.0	41.0
26-27	37.77706275429043	40.0	38.0	41.0	31.0	41.0
28-29	37.74263375901407	40.0	38.0	41.0	31.0	41.0
30-31	37.705314593843354	40.0	38.0	41.0	31.0	41.0
32-33	37.66524978552134	40.0	38.0	41.0	31.0	41.0
34-35	37.60964762238909	40.0	38.0	41.0	31.0	41.0
36-37	37.52704097795456	40.0	37.5	41.0	30.0	41.0
38-39	37.45425156801784	40.0	37.0	41.0	30.0	41.0
40-41	37.393169310889675	40.0	37.0	41.0	30.0	41.0
42-43	37.226799808076265	40.0	37.0	41.0	30.0	41.0
44-45	37.079165131779675	40.0	36.5	41.0	30.0	41.0
46-47	36.943797831904206	40.0	36.0	41.0	30.0	41.0
48-49	36.82697873899687	40.0	36.0	41.0	30.0	41.0
50-51	36.69782037126341	39.0	35.5	41.0	29.0	41.0
52-53	36.57209828499798	39.0	35.0	41.0	29.0	41.0
54-55	36.467685377445946	39.0	35.0	41.0	29.0	41.0
56-57	36.29317870269486	39.0	35.0	41.0	28.5	41.0
58-59	36.11305774792298	39.0	35.0	40.0	28.0	41.0
60-61	35.88683081563205	38.0	35.0	40.0	28.0	41.0
62-63	35.63188795235776	38.0	34.0	40.0	28.0	41.0
64-65	35.342062683730234	37.0	34.0	40.0	28.0	41.0
66-67	35.109634726905284	37.0	34.0	40.0	28.0	41.0
68-69	34.872187142180735	36.0	34.0	39.0	27.5	41.0
70-71	34.55932518663171	36.0	34.0	39.0	27.0	41.0
72-73	34.20275471865217	35.0	33.5	39.0	26.0	40.5
74-75	33.862816383395874	35.0	33.0	37.5	26.0	40.0
76-77	33.549939585874924	35.0	33.0	37.0	26.0	39.0
78-79	33.24849159336111	35.0	33.0	37.0	26.0	39.0
80-81	33.02310408407271	35.0	33.0	36.0	26.0	39.0
82-83	32.7682822680285	35.0	33.0	36.0	26.0	37.0
84-85	32.56210355511457	35.0	32.5	35.5	25.0	37.0
86-87	32.34012349493889	35.0	32.0	35.0	25.0	37.0
88-89	32.13922767244303	35.0	32.0	35.0	25.0	36.0
90-91	31.988917183256504	35.0	32.0	35.0	25.0	36.0
92-93	31.84425917392017	35.0	32.0	35.0	24.0	36.0
94-95	31.743705179069607	35.0	32.0	35.0	24.0	35.5
96-97	31.66204866414953	35.0	32.0	35.0	24.0	35.0
98-99	31.552726348587797	35.0	32.0	35.0	23.5	35.0
100	30.280982616401207	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21479341131377439
1101	2	0.1898927025118411
1101	3	0.26643670234137673
1101	4	0.2710000652158513
1101	5	0.17272327870328752
1101	6	0.18257161328568827
1101	7	0.25721316279323503
1101	8	0.2579506082341112
1101	9	0.42959670043993015
1101	10-11	0.4675479500071731
1101	12-13	0.40707703901586
1101	14-15	0.5835302588616216
1101	16-17	0.5373928933093453
1101	18-19	0.46705656136298757
1101	20-21	0.5240476626075932
1101	22-23	0.5118868022820635
1101	24-25	0.5821949485185698
1101	26-27	0.5132844745905416
1101	28-29	0.5324254867048452
1101	30-31	0.5181266519631151
1101	32-33	0.5840637313236599
1101	34-35	0.5263007224056437
1101	36-37	0.5383295843640852
1101	38-39	0.6118884935969504
1101	40-41	0.5231702122046755
1101	42-43	0.4832313323080797
1101	44-45	0.4400859783930784
1101	46-47	0.37267210180816335
1101	48-49	0.484926318314173
1101	50-51	0.4424523564248588
1101	52-53	0.3595854770093112
1101	54-55	0.4308538718206094
1101	56-57	0.44791462499521373
1101	58-59	0.466606426977215
1101	60-61	0.479957728035302
1101	62-63	0.44974994536553226
1101	64-65	0.5263834728150556
1101	66-67	0.4319408929729178
1101	68-69	0.42157710025463047
1101	70-71	0.423945346368491
1101	72-73	0.47610123691140416
1101	74-75	0.43113255274285933
1101	76-77	0.42324005849386026
1101	78-79	0.4008723330723569
1101	80-81	0.3329926016804876
1101	82-83	0.4194852321990581
1101	84-85	0.3630971898767612
1101	86-87	0.4441319961576511
1101	88-89	0.3944928206153122
1101	90-91	0.36941327487169673
1101	92-93	0.4144328801853625
1101	94-95	0.410889237648945
1101	96-97	0.3488670731383863
1101	98-99	0.3630516609145502
1101	100	0.3836741456278929
1102	1	0.29876169316791135
1102	2	0.3109492640170508
1102	3	0.3045371968860593
1102	4	0.34510200568701066
1102	5	0.325839660025089
1102	6	0.34875315801493656
1102	7	0.38850405032102486
1102	8	0.4308822208552101
1102	9	0.4534788073319831
1102	10-11	0.429303891353257
1102	12-13	0.4980809244041211
1102	14-15	0.574808283018676
1102	16-17	0.5412549071982724
1102	18-19	0.5018419592308234
1102	20-21	0.4913393158991042
1102	22-23	0.520509607077507
1102	24-25	0.5627924598860048
1102	26-27	0.5039907141216347
1102	28-29	0.5386025921279156
1102	30-31	0.5240940681857253
1102	32-33	0.4996579721035985
1102	34-35	0.5489811310142443
1102	36-37	0.5318660474746224
1102	38-39	0.5267431818012156
1102	40-41	0.4798799265379827
1102	42-43	0.5418865443703851
1102	44-45	0.39759420380034527
1102	46-47	0.4509083866994672
1102	48-49	0.44464298241778266
1102	50-51	0.4811994369091792
1102	52-53	0.41927148402096037
1102	54-55	0.41986431630033394
1102	56-57	0.44577028749115755
1102	58-59	0.39336077823548976
1102	60-61	0.4484073501219399
1102	62-63	0.41913752848017083
1102	64-65	0.5582754085565966
1102	66-67	0.5200063093677301
1102	68-69	0.399910300043814
1102	70-71	0.40909769204246516
1102	72-73	0.44008039805174093
1102	74-75	0.45221572354218154
1102	76-77	0.3937937557673479
1102	78-79	0.3654001201263384
1102	80-81	0.2885971676628287
1102	82-83	0.3495042817329974
1102	84-85	0.42265235679948177
1102	86-87	0.31226202469628106
1102	88-89	0.3030019718733925
1102	90-91	0.3084805792987808
1102	92-93	0.2302337909066985
1102	94-95	0.3807700051180518
1102	96-97	0.28718054157195283
1102	98-99	0.346701444116821
1102	100	0.29638706767510214
1103	1	0.15409622594323835
1103	2	0.1659142548464807
1103	3	0.18028460848367445
1103	4	0.1659938096094109
1103	5	0.1451416607437963
1103	6	0.12274736041755574
1103	7	0.14509990877627388
1103	8	0.1203816588612483
1103	9	0.2937140107652212
1103	10-11	0.25769350105134947
1103	12-13	0.2560114712113801
1103	14-15	0.37160845984363533
1103	16-17	0.3371271283942505
1103	18-19	0.351950304576242
1103	20-21	0.3260055395570518
1103	22-23	0.3016605578491536
1103	24-25	0.3374847227659359
1103	26-27	0.2791116014967301
1103	28-29	0.2754007652563786
1103	30-31	0.34642112381681756
1103	32-33	0.4146859576113755
1103	34-35	0.35351870865117974
1103	36-37	0.3218654380379746
1103	38-39	0.3761191621993163
1103	40-41	0.2759176135706767
1103	42-43	0.29030547894893743
1103	44-45	0.22796416717861945
1103	46-47	0.22035157673064987
1103	48-49	0.21167247839474612
1103	50-51	0.2662659550903257
1103	52-53	0.25728300073341615
1103	54-55	0.11008717643323251
1103	56-57	0.18613839669311716
1103	58-59	0.3076663194909415
1103	60-61	0.18805337783150122
1103	62-63	0.21345557580476537
1103	64-65	0.1964240358983247
1103	66-67	0.24667087896665407
1103	68-69	0.19706072945060527
1103	70-71	0.17015814714906696
1103	72-73	0.1686802286964948
1103	74-75	0.14929476179814571
1103	76-77	0.15066179456235318
1103	78-79	0.18973155045270573
1103	80-81	0.16878455555423955
1103	82-83	0.2107449401920789
1103	84-85	0.2156870699579727
1103	86-87	0.15671446983689208
1103	88-89	0.08384269983096715
1103	90-91	0.17429461506652189
1103	92-93	0.11407470737324488
1103	94-95	0.11967221728477995
1103	96-97	0.20788404786416592
1103	98-99	0.16213117203300698
1103	100	0.15335215894171128
1104	1	0.13146250147191907
1104	2	0.11103191086216668
1104	3	0.11858869172790776
1104	4	0.12113728851351624
1104	5	0.09936922807015947
1104	6	0.12174459800253601
1104	7	0.16242294321543227
1104	8	0.1850595944615776
1104	9	0.2981142830679602
1104	10-11	0.27087222248213294
1104	12-13	0.29327995203682633
1104	14-15	0.369551133727839
1104	16-17	0.3097693848420491
1104	18-19	0.3472849916178191
1104	20-21	0.3480218665537933
1104	22-23	0.33135168589053166
1104	24-25	0.3252069174088348
1104	26-27	0.31400463837120185
1104	28-29	0.3212820992131924
1104	30-31	0.34691348370507313
1104	32-33	0.34444667139616314
1104	34-35	0.30125023563120124
1104	36-37	0.33790360245137663
1104	38-39	0.31543740083912297
1104	40-41	0.3862127881345643
1104	42-43	0.40277992504893945
1104	44-45	0.34597259972544947
1104	46-47	0.30439393171947415
1104	48-49	0.29076326991238943
1104	50-51	0.3422385523752709
1104	52-53	0.34553723105666734
1104	54-55	0.2421263122070556
1104	56-57	0.2915597172197195
1104	58-59	0.27910452252340434
1104	60-61	0.323284829758947
1104	62-63	0.31877371405423816
1104	64-65	0.31003243631064237
1104	66-67	0.2916036534765425
1104	68-69	0.2556081307342595
1104	70-71	0.1913184094896252
1104	72-73	0.256604903172466
1104	74-75	0.25668625739297823
1104	76-77	0.21422170200631285
1104	78-79	0.2439560436256869
1104	80-81	0.2345458150012547
1104	82-83	0.1975770113212647
1104	84-85	0.17897516400208247
1104	86-87	0.15742856504630254
1104	88-89	0.21103277893757877
1104	90-91	0.20964389653121174
1104	92-93	0.20058680236555304
1104	94-95	0.19699418477657105
1104	96-97	0.2047599544992451
1104	98-99	0.16992089357715656
1104	100	0.15284228178295933
1105	1	0.04348746888108934
1105	2	0.016066797219362883
1105	3	0.040671614133394485
1105	4	0.06044296215178946
1105	5	0.13483516356865977
1105	6	0.046128716349755905
1105	7	0.0999982138610136
1105	8	0.11316555716633303
1105	9	0.21009112940928532
1105	10-11	0.25382626941292585
1105	12-13	0.23893590623962524
1105	14-15	0.28126029600182534
1105	16-17	0.23237571596487072
1105	18-19	0.22157954186437934
1105	20-21	0.25276330791863444
1105	22-23	0.22732157479831727
1105	24-25	0.2849289318054815
1105	26-27	0.2522238896323259
1105	28-29	0.24727576525637573
1105	30-31	0.3332113780541093
1105	32-33	0.29409344348707833
1105	34-35	0.2187672962217988
1105	36-37	0.2816578109193273
1105	38-39	0.24910715654959148
1105	40-41	0.20873752882491914
1105	42-43	0.23929841680204333
1105	44-45	0.2129768790430333
1105	46-47	0.21638194396228982
1105	48-49	0.20854818460943392
1105	50-51	0.13811623757620595
1105	52-53	0.21985079734358237
1105	54-55	0.16188590524679114
1105	56-57	0.15525492211684622
1105	58-59	0.08706603700506577
1105	60-61	0.1922023891309408
1105	62-63	0.12180797693470424
1105	64-65	0.1935116065197917
1105	66-67	0.1772245512830395
1105	68-69	0.14000281984608876
1105	70-71	0.15216238443720442
1105	72-73	0.1549252851936629
1105	74-75	0.18158148496198123
1105	76-77	0.12211447817816179
1105	78-79	0.19988974254310676
1105	80-81	0.1380056007519812
1105	82-83	0.1887979062937717
1105	84-85	0.1275670134607907
1105	86-87	0.12412619300073402
1105	88-89	0.12360470548068747
1105	90-91	0.08998105574449511
1105	92-93	0.17195818194951684
1105	94-95	0.21430427943167274
1105	96-97	0.2604652625534314
1105	98-99	0.26238894039459026
1105	100	0.23547080300950896
1106	1	0.1357045627549951
1106	2	0.10251932974853872
1106	3	0.07656031687533726
1106	4	0.13307768600279246
1106	5	0.05372445267347814
1106	6	0.13504696061177413
1106	7	0.17771729019987959
1106	8	0.17311968125188315
1106	9	0.2556114566556147
1106	10-11	0.23835722099610734
1106	12-13	0.29712030340274964
1106	14-15	0.2666848366842416
1106	16-17	0.3450449793814059
1106	18-19	0.33083800224300575
1106	20-21	0.34933675684922605
1106	22-23	0.36244243535563925
1106	24-25	0.41650878884463083
1106	26-27	0.37306153692109234
1106	28-29	0.34831132397643216
1106	30-31	0.4218535989732999
1106	32-33	0.37371694267734057
1106	34-35	0.3286002386465441
1106	36-37	0.4121643446770804
1106	38-39	0.353678581132165
1106	40-41	0.3017125665196758
1106	42-43	0.34188725077758875
1106	44-45	0.3327083527683854
1106	46-47	0.3170900190167387
1106	48-49	0.3104003513598741
1106	50-51	0.3615299863895203
1106	52-53	0.29164684079027126
1106	54-55	0.2226215672784022
1106	56-57	0.29742555371342405
1106	58-59	0.3174013807253999
1106	60-61	0.3560035562722774
1106	62-63	0.3238216308130717
1106	64-65	0.3505548298402843
1106	66-67	0.31599344054422573
1106	68-69	0.16151398866275457
1106	70-71	0.1945875673265718
1106	72-73	0.2393359346603532
1106	74-75	0.2494544675944752
1106	76-77	0.24473634392899157
1106	78-79	0.2648518104495139
1106	80-81	0.2382396433051781
1106	82-83	0.2261379269560848
1106	84-85	0.21191425197087455
1106	86-87	0.14749044999520322
1106	88-89	0.18721008326591004
1106	90-91	0.21725326772862985
1106	92-93	0.21097603809515775
1106	94-95	0.16134744690808134
1106	96-97	0.18994095641616582
1106	98-99	0.26046099322309146
1106	100	0.366931405008728
1107	1	0.02249085067796841
1107	2	0.06532176340139273
1107	3	0.006259780507146928
1107	4	0.051994860751136684
1107	5	0.10148348332154455
1107	6	0.11586557527290609
1107	7	0.06929951332312356
1107	8	0.0808645239874437
1107	9	0.14575484033222352
1107	10-11	0.1767640789671674
1107	12-13	0.17442549456576728
1107	14-15	0.18444609511777088
1107	16-17	0.15960868715303178
1107	18-19	0.1563217131377499
1107	20-21	0.23906560594281956
1107	22-23	0.18762682856621637
1107	24-25	0.27730819899406356
1107	26-27	0.25448129230580463
1107	28-29	0.2762170897491103
1107	30-31	0.21999993586419464
1107	32-33	0.24934177921696232
1107	34-35	0.24621915221583635
1107	36-37	0.27316260401727277
1107	38-39	0.2232971496262337
1107	40-41	0.21023105814270338
1107	42-43	0.23605992023709632
1107	44-45	0.2273000669857268
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2216	40-41	-0.4442226411166601
2216	42-43	-0.3536016097513226
2216	44-45	-0.2292349283565116
2216	46-47	-0.28309468907011137
2216	48-49	-0.19933068775735308
2216	50-51	-0.272586541676759
2216	52-53	-0.21774660927706435
2216	54-55	-0.28559515334764995
2216	56-57	-0.2586906434699827
2216	58-59	-0.2748024352909155
2216	60-61	-0.25543214795527547
2216	62-63	-0.3364838446266347
2216	64-65	-0.34956486719237034
2216	66-67	-0.2926211736240276
2216	68-69	-0.362346534021853
2216	70-71	-0.4018862524896889
2216	72-73	-0.2952180145053944
2216	74-75	-0.23543294237028078
2216	76-77	-0.249534796029657
2216	78-79	-0.18053506715772727
2216	80-81	-0.2549158012660726
2216	82-83	-0.23280162459665377
2216	84-85	-0.21692015240927276
2216	86-87	-0.3065807905361737
2216	88-89	-0.24697495463615127
2216	90-91	-0.25448796532118223
2216	92-93	-0.1845298247773357
2216	94-95	-0.24093753327854017
2216	96-97	-0.27390387357685597
2216	98-99	-0.26359641099503506
2216	100	-0.153898111842679
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1279.0
1	300.0
2	232.0
3	214.0
4	183.0
5	248.0
6	380.0
7	730.0
8	2264.0
9	3822.0
10	4943.0
11	6814.0
12	9290.0
13	10939.0
14	13081.0
15	14000.0
16	14509.0
17	15187.0
18	16722.0
19	17844.0
20	19883.0
21	21871.0
22	23938.0
23	27570.0
24	32544.0
25	38367.0
26	42053.0
27	42470.0
28	46356.0
29	55893.0
30	66158.0
31	77835.0
32	97119.0
33	130709.0
34	191403.0
35	293899.0
36	517140.0
37	931599.0
38	1030550.0
39	289137.0
40	491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10123958790485	19.707664948385816	13.144239635152294	24.04685582855704
2	14.443629376624065	21.229470699069307	39.909080307282466	24.41781961702417
3	18.56480914437548	26.760866214418577	30.262716707619642	24.4116079335863
4	12.117260655045504	17.717085913906384	39.548551299527645	30.61710213152047
5	12.963815697356582	38.650007366164104	35.40375710895714	12.982419827522182
6	29.16519433015487	38.02242172669457	18.952021109104702	13.860362834045858
7	25.997980210740923	33.154229442117845	23.27981240848058	17.56797793866065
8	25.024096989958412	35.075963356015436	21.261727648245802	18.638212005780357
9	26.03911530959124	16.667774562849733	21.022269200266965	36.270840927292056
10-11	23.889670619258293	26.538021864884122	28.939431000670744	20.632876515186844
12-13	25.534441949812532	24.25597893958205	28.03933537551731	22.170243735088107
14-15	23.20044366901123	25.633841572175964	26.02897971955346	25.13673503925935
16-17	22.128067222757203	28.478306369123445	27.25930094478734	22.134325463332004
18-19	22.041043288703452	29.00419106748152	27.74139182596952	21.213373817845508
20-21	22.972785090602734	27.896609154260837	26.788801394712365	22.341804360424064
22-23	22.634826319846926	27.733367715317176	26.035988586739755	23.595817378096143
24-25	22.773306093327864	27.96952788184201	26.471976925540062	22.78518909929006
26-27	23.13773911065925	27.34262186649577	26.672041278185212	22.84759774465977
28-29	22.50073600691956	27.62682635033832	27.29515448109503	22.577283161647088
30-31	22.907655910783927	28.031662500441378	26.724336316050294	22.336345272724394
32-33	22.36407269360432	28.300281137168128	26.46606476273321	22.869581406494344
34-35	22.66852204610621	27.87870367619375	26.85798115948876	22.594793118211275
36-37	23.29893467664654	27.48581740340675	26.901649065513304	22.31359885443341
38-39	23.54552378702577	27.18692750130748	27.01279961716219	22.25474909450456
40-41	22.668577530626386	27.48119069391393	27.422939377962642	22.427292397497045
42-43	22.754598933541804	27.711656882478934	26.799298119999076	22.734446063980187
44-45	22.85087614623267	27.86326205873056	26.51195316050204	22.773908634534727
46-47	22.622954412624196	27.537149327878435	26.78404441846873	23.055851841028637
48-49	23.0963167992265	27.41055636162379	26.94632194412699	22.54680489502272
50-51	22.833258953441433	27.64902107741455	26.886120833939636	22.63159913520438
52-53	22.186065871021277	27.84145527946998	26.85384783434118	23.118631015167562
54-55	22.17621667944997	27.58690767542356	26.942393075367576	23.294482569758895
56-57	22.99887128633538	27.467245201931856	26.39915012208349	23.134733389649274
58-59	22.710336687757042	27.20874163565785	26.99483806165976	23.086083614925343
60-61	22.768312011257034	27.264768615235113	27.048663775283117	22.91825559822474
62-63	22.8613233086003	27.16344625771309	26.815892952607808	23.159337481078804
64-65	22.337102876362554	27.385293119407777	27.261333076458342	23.016270927771323
66-67	22.689700408558142	27.160640267650155	27.03080724210346	23.118852081688246
68-69	22.504552123642398	27.4381505469368	26.860890608742416	23.196406720678382
70-71	22.43540562408633	27.346385437993735	26.604453128310944	23.61375580960899
72-73	22.755721195402927	27.048581419245664	26.679313726899	23.516383658452412
74-75	22.84753908519627	27.405677283304087	26.661354511806028	23.085429119693615
76-77	22.77105329110696	26.842055654345177	27.173866185137296	23.21302486941057
78-79	22.613203330609384	26.85876818115943	27.4104840880222	23.117544400208985
80-81	22.33321090263252	27.51222439696385	27.179956567952452	22.974608132451184
82-83	22.418514449479527	27.112696940235587	27.053630245168193	23.415158365116696
84-85	22.587988233892904	27.105242454924156	26.60615472523383	23.70061458594911
86-87	22.63557176382393	26.989774737841195	26.969669329973584	23.4049841683613
88-89	23.067288654135584	26.23776127376643	27.18228692307758	23.51266314902041
90-91	22.83016664436104	26.570936345660854	27.318530108154494	23.28036690182361
92-93	22.536810244765174	27.11140466111292	27.08627106582518	23.265514028296728
94-95	22.019263026958374	27.115824748952917	27.189995744087543	23.67491648000117
96-97	22.322525684336217	26.738790991708655	26.928396803770344	24.01028652018478
98-99	22.597825524361618	26.83788860912111	26.657208012251598	23.907077854265683
100	22.55362514754096	27.00218478921309	26.686211143278705	23.757978919967247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2881.0
1	1857.5
2	850.5
3	836.5
4	795.5
5	692.5
6	573.0
7	554.5
8	623.0
9	649.5
10	575.0
11	515.0
12	505.5
13	511.5
14	562.0
15	662.5
16	768.0
17	974.5
18	1231.0
19	1607.0
20	2230.0
21	3201.5
22	4585.5
23	6391.5
24	8720.0
25	11911.0
26	16709.5
27	22518.0
28	28933.5
29	36375.0
30	44943.5
31	53583.0
32	63372.0
33	73805.5
34	82809.5
35	90958.5
36	100489.0
37	111181.0
38	117306.0
39	119421.5
40	120718.5
41	121593.5
42	123001.0
43	126849.0
44	132817.5
45	140816.5
46	152153.5
47	168186.5
48	217868.0
49	227107.5
50	203552.0
51	190733.0
52	157235.0
53	139517.0
54	129221.5
55	116108.5
56	104226.5
57	91889.5
58	80259.5
59	71574.0
60	60360.5
61	48390.0
62	39108.5
63	31198.0
64	23242.5
65	16747.5
66	12449.5
67	9361.5
68	7271.5
69	5634.0
70	4557.5
71	3802.5
72	3236.0
73	3058.0
74	2600.0
75	2050.5
76	1578.0
77	1129.0
78	767.5
79	468.0
80	298.5
81	203.0
82	135.0
83	104.0
84	99.0
85	94.0
86	71.5
87	48.5
88	40.0
89	36.0
90	29.0
91	20.0
92	15.0
93	17.5
94	17.0
95	12.5
96	10.0
97	14.5
98	11.0
99	5.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09547524237426783
2	0.06338251946609777
3	0.07841914020699928
4	0.08077925705468122
5	0.08153352120187855
6	0.08956278470430168
7	0.08751897217641216
8	0.0886625339479694
9	0.07501278599385007
10-11	0.0811563891282799
12-13	0.07116847195329597
14-15	0.08031696612575384
16-17	0.08225128869679214
18-19	0.08211746763841841
20-21	0.07362591320706789
22-23	0.07698360521717211
24-25	0.07934372206485407
26-27	0.07305413232128928
28-29	0.08143619679578858
30-31	0.08591311947592754
32-33	0.08777444874239836
34-35	0.09021972444540904
36-37	0.09004940673475158
38-39	0.09217837811796983
40-41	0.08810291861295202
42-43	0.09369907196312573
44-45	0.08973310241495916
46-47	0.08676470802921483
48-49	0.09631466537679387
50-51	0.09230003362558231
52-53	0.09812733244021969
54-55	0.08996424787942284
56-57	0.09646065198592883
58-59	0.10082808470921659
60-61	0.09947770857471813
62-63	0.1002684693741992
64-65	0.10237310965589495
66-67	0.10481838535890564
68-69	0.10194731537925131
70-71	0.10384514129800587
72-73	0.1006577669985591
74-75	0.10431959777769452
76-77	0.10119305123205398
78-79	0.10732448881572255
80-81	0.10321253265842102
82-83	0.10796926300606867
84-85	0.10401545900866334
86-87	0.09989133730060054
88-89	0.0996480262853756
90-91	0.10040229043257291
92-93	0.0950859447499079
94-95	0.09521976580828162
96-97	0.09283531785907717
98-99	0.10653372801624149
100	0.24956410831622453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4109966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.23080099872383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73028542311702	42.351427770539125
2	14.33390358640279	16.693493745494294
3	5.543104436976784	9.683382341542146
4	2.734561913814964	6.369429224881943
5	1.476182918757839	4.297965688995151
6	0.918019083813785	3.207419194955477
7	0.5750893748693934	2.3441540461150097
8	0.39527964407100175	1.8413960234195912
9	0.2808071042719466	1.4716460347098854
>10	0.982801986059936	8.935966284827941
>50	0.022297694217595535	0.8730680408485328
>100	0.007257347055432993	0.6995083979826325
>500	6.82477619176041E-5	0.024202402022848627
>1k	2.047432857523364E-4	0.2718299340490966
>5k	6.824776191744548E-5	0.2934094156556258
>10k+	6.824776191744548E-5	0.6417014539606379
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	16147	0.39287429628371623	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	7383	0.17963652254057577	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8662203044988696E-5
2	0.0	0.0	0.0	0.0	4.8662203044988696E-5
3	0.0	0.0	0.0	0.0	4.8662203044988696E-5
4	0.0	0.0	0.0	0.0	4.8662203044988696E-5
5	0.0	0.0	0.0	0.0	4.8662203044988696E-5
6	0.0	0.0	0.0	0.0	4.8662203044988696E-5
7	0.0	0.0	0.0	0.0	4.8662203044988696E-5
8	0.0	0.0	0.0	0.0	4.8662203044988696E-5
9	0.0	0.0	0.0	0.0	4.8662203044988696E-5
10-11	0.0	0.0	0.0	2.4331101522494348E-5	7.299330456748303E-5
12-13	0.0	0.0	0.0	2.4331101522494348E-5	2.0681436294120196E-4
14-15	0.0	0.0	0.0	2.4331101522494348E-5	2.311454644636963E-4
16-17	0.0	0.0	0.0	4.8662203044988696E-5	2.554765659861906E-4
18-19	0.0	0.0	0.0	4.8662203044988696E-5	3.163043197924265E-4
20-21	0.0	0.0	0.0	8.51588553287302E-5	4.257942766436511E-4
22-23	0.0	0.0	0.0	3.771320735986624E-4	4.3795982740489826E-4
24-25	0.0	0.0	0.0	7.664296979585719E-4	4.3795982740489826E-4
26-27	0.0	0.0	0.0	0.0017761704111420875	4.5012537816614543E-4
28-29	0.0	0.0	0.0	0.005292014581142521	4.622909289273926E-4
30-31	0.0	0.0	0.0	0.013966052273911756	4.622909289273926E-4
32-33	0.0	0.0	0.0	0.03425819094367204	4.622909289273926E-4
34-35	0.0	0.0	0.0	0.06920981828073516	5.352842334948756E-4
36-37	0.0	0.0	0.0	0.12393046560482496	5.352842334948756E-4
38-39	0.0	0.0	0.0	0.20081674641590708	5.474497842561227E-4
40-41	0.0	0.0	0.0	0.2909756431075099	5.596153350173699E-4
42-43	0.0	0.0	0.0	0.3774240468169323	5.596153350173699E-4
44-45	0.0	0.0	0.0	0.48107453930275823	5.717808857786172E-4
46-47	0.0	0.0	0.0	0.6148955976764772	5.839464365398643E-4
48-49	0.0	0.0	0.0	0.7617216298139693	6.32608639584853E-4
50-51	0.0	0.0	0.0	0.9130124190808391	6.812708426298418E-4
52-53	0.0	0.0	0.0	1.0660550476573287	7.056019441523361E-4
54-55	0.0	0.0	0.0	1.2361172817488029	7.056019441523361E-4
56-57	0.0	0.0	0.0	1.4347807256799692	7.177674949135832E-4
58-59	0.0	0.0	0.0	1.6761574183338743	7.299330456748304E-4
60-61	0.0	0.0	0.0	1.9328262082946672	7.299330456748304E-4
62-63	0.0	0.0	0.0	2.184920751169231	7.542641471973248E-4
64-65	0.0	0.0	0.0	2.442477626335595	7.785952487198191E-4
66-67	0.0	0.0	0.0	2.735728227435458	7.785952487198191E-4
68-69	1.2165550761247174E-5	0.0	0.0	3.0735777376260534	7.785952487198191E-4
70-71	2.4331101522494348E-5	0.0	0.0	3.452303498374439	7.785952487198191E-4
72-73	2.4331101522494348E-5	0.0	0.0	3.8279513747802296	8.150919010035606E-4
74-75	2.4331101522494348E-5	0.0	0.0	4.200022579262212	8.759196548097965E-4
76-77	2.4331101522494348E-5	0.0	0.0	4.59934461744939	9.12416307093538E-4
78-79	2.4331101522494348E-5	0.0	0.0	5.044810589673978	9.367474086160324E-4
80-81	2.4331101522494348E-5	1.2165550761247174E-5	0.0	5.527843782649297	9.489129593772795E-4
82-83	2.4331101522494348E-5	2.4331101522494348E-5	0.0	6.035998837946591	9.610785101385267E-4
84-85	2.4331101522494348E-5	2.4331101522494348E-5	0.0	6.5522561500508765	9.854096116610211E-4
86-87	2.4331101522494348E-5	2.4331101522494348E-5	0.0	7.090958903309662	0.0010340718147060098
88	2.4331101522494348E-5	2.4331101522494348E-5	0.0	7.508748247552413	0.0010705684669897512
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4360	0.0	19.623383	2
GTACTAG	1690	0.0	19.476759	1
CATGGGG	7935	0.0	19.076233	4
CTAACGC	655	0.0	18.660402	3
TAACGCC	765	0.0	17.206015	4
ATGGGGG	4905	0.0	16.77151	5
GTATAAG	2405	0.0	16.619165	1
TCTAACG	720	0.0	16.322868	2
GTACCTG	5405	0.0	16.26864	1
GTACCCG	495	1.8189894E-12	16.149128	1
ACCTGGG	4890	0.0	15.958358	3
TAGGCAT	2295	0.0	15.566966	5
CAGTACG	485	2.910383E-11	15.503119	9
GTATATA	2610	0.0	15.493991	1
TACGGGC	945	0.0	15.421037	4
CGGGCGT	950	0.0	15.335202	6
CTAGTAC	2095	0.0	15.258572	3
TAGTACT	2335	0.0	15.099343	4
GGCGTCG	1000	0.0	15.038392	8
TGGGGGG	4635	0.0	14.701772	6
>>END_MODULE