FastQCFastQC Report
Mon 16 Jan 2017
ERR1146583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109966
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA160430.39034386172537683No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA128020.3114867616909726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113960.2772772329503456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111270.27073216664079464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.14026880027717992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT129300.022.20851512-13
GTACTTT141700.020.1822814-15
AGTACTT137300.019.57953312-13
GTACCCG5450.018.9810281
GTACTAG16550.018.1834161
TACCTGG44550.018.0485342
TAGTACT22900.017.4530624
ACTTTTT166450.017.44965416-17
CTGTGCG20550.017.3811559
CTAGTAC21550.017.2372533
ACCTGGG47700.016.6592923
TACTTTT162800.016.41177614-15
CATGGGG74500.016.3467624
ACCGTAT2607.3351766E-616.268648
GTATAGA14800.016.2032431
TAACGCC6750.016.0218374
TAGGCAT20050.015.4715145
CTAACGC6200.015.1679373
GTCTATA14700.015.0339831
TGATCGC9100.014.9772979
Produced by FastQC (version 0.11.5)