##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4109966
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28861114666155	33.0	31.0	34.0	30.0	34.0
2	32.41453092312686	34.0	31.0	34.0	30.0	34.0
3	32.5189184046778	34.0	31.0	34.0	30.0	34.0
4	36.005055272963325	37.0	35.0	37.0	35.0	37.0
5	36.05681385198807	37.0	35.0	37.0	35.0	37.0
6	36.12968769084708	37.0	36.0	37.0	35.0	37.0
7	36.10072370428369	37.0	36.0	37.0	35.0	37.0
8	36.10740843111598	37.0	36.0	37.0	35.0	37.0
9	37.8897192823493	39.0	38.0	39.0	35.0	39.0
10-11	37.83890937297292	39.0	38.0	39.0	35.0	39.0
12-13	37.81984108870974	39.0	38.0	39.0	35.0	39.0
14-15	39.16050290926981	40.0	39.0	41.0	36.0	41.0
16-17	39.09262242072076	40.0	39.0	41.0	36.0	41.0
18-19	39.20485388443603	40.0	39.0	41.0	36.0	41.0
20-21	39.26375546659024	40.0	39.0	41.0	36.0	41.0
22-23	39.23552202135005	40.5	39.0	41.0	36.0	41.0
24-25	39.23109826212674	41.0	39.0	41.0	36.0	41.0
26-27	39.138984118116795	40.5	39.0	41.0	35.5	41.0
28-29	39.07527166891405	40.0	39.0	41.0	35.0	41.0
30-31	38.961247732949616	40.0	38.5	41.0	35.0	41.0
32-33	38.858108680217796	40.0	38.0	41.0	35.0	41.0
34-35	38.80246612745701	40.0	38.0	41.0	35.0	41.0
36-37	38.74284251986513	40.0	38.0	41.0	35.0	41.0
38-39	38.65426599636104	40.0	38.0	41.0	35.0	41.0
40-41	38.564223767301236	40.0	38.0	41.0	35.0	41.0
42-43	38.48747179417056	40.0	38.0	41.0	34.5	41.0
44-45	38.372722548069746	40.0	38.0	41.0	34.0	41.0
46-47	38.16013563129233	40.0	38.0	41.0	33.5	41.0
48-49	37.899470944528495	40.0	37.5	41.0	33.0	41.0
50-51	37.79368466795103	40.0	37.0	41.0	33.0	41.0
52-53	37.682620853797815	40.0	37.0	41.0	33.0	41.0
54-55	37.53979351654004	40.0	37.0	41.0	33.0	41.0
56-57	37.40740178872526	40.0	36.0	41.0	33.0	41.0
58-59	37.24586602419582	39.5	36.0	41.0	32.0	41.0
60-61	37.02802054810186	39.0	35.0	41.0	32.0	41.0
62-63	36.76848591934824	39.0	35.0	41.0	31.5	41.0
64-65	36.49774280371176	38.5	35.0	40.5	31.0	41.0
66-67	36.22980567235837	37.5	35.0	40.0	31.0	41.0
68-69	35.94415270101991	37.0	35.0	40.0	31.0	41.0
70-71	35.6191421048252	36.5	35.0	39.0	31.0	41.0
72-73	35.27000600491586	36.0	35.0	39.0	30.5	41.0
74-75	34.94099099603257	35.5	35.0	38.5	30.0	40.5
76-77	34.59873573163379	35.0	34.0	37.0	30.0	39.5
78-79	34.30167110871477	35.0	34.0	37.0	30.0	39.0
80-81	34.057358017073625	35.0	34.0	36.5	30.0	39.0
82-83	33.81490990436417	35.0	34.0	36.0	29.5	37.5
84-85	33.603248542688675	35.0	34.0	36.0	29.0	37.0
86-87	33.40298253075573	35.0	34.0	35.0	29.0	37.0
88-89	33.18782965601175	35.0	34.0	35.0	29.0	36.0
90-91	33.087435394842686	35.0	34.0	35.0	29.0	36.0
92-93	32.99293826274962	35.0	34.0	35.0	29.0	36.0
94-95	32.93470882727497	35.0	34.0	35.0	29.0	36.0
96-97	32.881545613759336	35.0	34.0	35.0	29.0	35.5
98-99	32.807892328063055	35.0	34.0	35.0	29.0	35.0
100	32.02664255616713	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31563433085948844
1101	2	0.34605259023842194
1101	3	0.3434586393376833
1101	4	0.2118727384991672
1101	5	0.23262267873789
1101	6	0.2576310471611052
1101	7	0.259876805704927
1101	8	0.27144775134485144
1101	9	0.33982793221187535
1101	10-11	0.33522628881294025
1101	12-13	0.3235059604473207
1101	14-15	0.49944845395378223
1101	16-17	0.4866502702462512
1101	18-19	0.4383081403047768
1101	20-21	0.4120731406232494
1101	22-23	0.4157913350075404
1101	24-25	0.3936210977774621
1101	26-27	0.3868084999290673
1101	28-29	0.40909337839537585
1101	30-31	0.46568075278367616
1101	32-33	0.41095454937978104
1101	34-35	0.3945445046059959
1101	36-37	0.4746168483151436
1101	38-39	0.43884526197312823
1101	40-41	0.4479607920096029
1101	42-43	0.4735132344626365
1101	44-45	0.44525991140915266
1101	46-47	0.4774331462333592
1101	48-49	0.47644455185304224
1101	50-51	0.4313602844069848
1101	52-53	0.39338309478438305
1101	54-55	0.48040996208336395
1101	56-57	0.39277776826131117
1101	58-59	0.3494487978025731
1101	60-61	0.3581439596511302
1101	62-63	0.3917479926765921
1101	64-65	0.39075373344108755
1101	66-67	0.3908642058820391
1101	68-69	0.34812353913114435
1101	70-71	0.2764511123079032
1101	72-73	0.28981100486175393
1101	74-75	0.2879790025195348
1101	76-77	0.35144777736995536
1101	78-79	0.2851434745118979
1101	80-81	0.2687543341929697
1101	82-83	0.25151609914482975
1101	84-85	0.26611807245306807
1101	86-87	0.2617997563862957
1101	88-89	0.3519300475953884
1101	90-91	0.3386261802816364
1101	92-93	0.3123628214427825
1101	94-95	0.3482177241076272
1101	96-97	0.3167930800493366
1101	98-99	0.3391761816315224
1101	100	0.39458622978170155
1102	1	0.3080510138251782
1102	2	0.2779618286568706
1102	3	0.28760742311543197
1102	4	0.2300169260356313
1102	5	0.20005223917524262
1102	6	0.23098489154882174
1102	7	0.2165836169045221
1102	8	0.20761208795640584
1102	9	0.274685128723668
1102	10-11	0.30702178079373255
1102	12-13	0.29834859815829873
1102	14-15	0.4400629959330118
1102	16-17	0.537659872319523
1102	18-19	0.3985521296152754
1102	20-21	0.3694915679575601
1102	22-23	0.3817618302108201
1102	24-25	0.41061509691714093
1102	26-27	0.4060279584233015
1102	28-29	0.41199498588539996
1102	30-31	0.4969010195103323
1102	32-33	0.5131145969845434
1102	34-35	0.4679172442468271
1102	36-37	0.4697713854403389
1102	38-39	0.47708231992747585
1102	40-41	0.5107060881449925
1102	42-43	0.4890305504939576
1102	44-45	0.5329641779705767
1102	46-47	0.5462729992584769
1102	48-49	0.6501405346956872
1102	50-51	0.6510466829269532
1102	52-53	0.5270037719975562
1102	54-55	0.508900420478227
1102	56-57	0.4600098492491185
1102	58-59	0.41541162873286197
1102	60-61	0.41084784714623623
1102	62-63	0.48582989654835984
1102	64-65	0.45122894490949506
1102	66-67	0.4264424080924343
1102	68-69	0.46439059359011736
1102	70-71	0.4788408060836957
1102	72-73	0.4698724484425014
1102	74-75	0.43789095462148
1102	76-77	0.43847835101756516
1102	78-79	0.48850412550396527
1102	80-81	0.40003579897820885
1102	82-83	0.39534751234552346
1102	84-85	0.40450899649398764
1102	86-87	0.4483116312814701
1102	88-89	0.4688980346570304
1102	90-91	0.4589014670324474
1102	92-93	0.42719421822003767
1102	94-95	0.4614544378332539
1102	96-97	0.4650948712496614
1102	98-99	0.46647396634143234
1102	100	0.48933814373481255
1103	1	0.2621503711786417
1103	2	0.26267843808948754
1103	3	0.27486404591275715
1103	4	0.2319867754505225
1103	5	0.20503633956599288
1103	6	0.17123246667230063
1103	7	0.16812299332590896
1103	8	0.1777107346789748
1103	9	0.22014903367072947
1103	10-11	0.2432862836196037
1103	12-13	0.2359138476260867
1103	14-15	0.30338840406630396
1103	16-17	0.43627601580438125
1103	18-19	0.29043484977709966
1103	20-21	0.3037859374694776
1103	22-23	0.3210492902504569
1103	24-25	0.3081805299729865
1103	26-27	0.3560530961080346
1103	28-29	0.3933010212513963
1103	30-31	0.4329303908240547
1103	32-33	0.38314680501770226
1103	34-35	0.34012363015876446
1103	36-37	0.3282421984717274
1103	38-39	0.37784311578865726
1103	40-41	0.4171390453183861
1103	42-43	0.4434364557458679
1103	44-45	0.4307514781434918
1103	46-47	0.4594315567585099
1103	48-49	0.4967575682356511
1103	50-51	0.397595353572747
1103	52-53	0.3744199025366157
1103	54-55	0.43318155966295535
1103	56-57	0.431262829558392
1103	58-59	0.39778004593375726
1103	60-61	0.4261233200842227
1103	62-63	0.4069056418275565
1103	64-65	0.4016495539878022
1103	66-67	0.38217235548151507
1103	68-69	0.3859137953356111
1103	70-71	0.38773510503722974
1103	72-73	0.3690696859910716
1103	74-75	0.3621981336590778
1103	76-77	0.3249811524073323
1103	78-79	0.3113674980077974
1103	80-81	0.32713969922138375
1103	82-83	0.29304811289929944
1103	84-85	0.2721018649243092
1103	86-87	0.2768160150615273
1103	88-89	0.39971270068069487
1103	90-91	0.3766601198973305
1103	92-93	0.3850187886618315
1103	94-95	0.37137728731678976
1103	96-97	0.376922588807183
1103	98-99	0.31166129997657777
1103	100	0.26896086585491474
1104	1	0.23987377686985667
1104	2	0.22289327938151615
1104	3	0.17518951589796927
1104	4	0.18148891270762846
1104	5	0.13415143610881586
1104	6	0.16724987694784943
1104	7	0.17538441980993014
1104	8	0.20186730587396085
1104	9	0.24668843398986695
1104	10-11	0.2409212569657342
1104	12-13	0.2205860558144792
1104	14-15	0.32833723022005046
1104	16-17	0.4532731719723273
1104	18-19	0.3606972071531942
1104	20-21	0.30559239285534545
1104	22-23	0.3380852936301011
1104	24-25	0.3028019623939642
1104	26-27	0.31583270753024095
1104	28-29	0.3582863241563743
1104	30-31	0.4010570341751958
1104	32-33	0.3695963331831891
1104	34-35	0.33762226300110143
1104	36-37	0.3313672825306142
1104	38-39	0.34436475986990445
1104	40-41	0.3656393808334073
1104	42-43	0.34351497274545295
1104	44-45	0.32247632856671515
1104	46-47	0.39036601772973256
1104	48-49	0.39248012397149523
1104	50-51	0.3255976005867396
1104	52-53	0.3026479527212018
1104	54-55	0.3348843194121045
1104	56-57	0.325114865032198
1104	58-59	0.29941896596446327
1104	60-61	0.3007380301963636
1104	62-63	0.32167575999993403
1104	64-65	0.2634874491966386
1104	66-67	0.24175520607670364
1104	68-69	0.2803857050189009
1104	70-71	0.29216084718108704
1104	72-73	0.25890618366096163
1104	74-75	0.2716747938700266
1104	76-77	0.24921627062293794
1104	78-79	0.2146060735652071
1104	80-81	0.21564763125761033
1104	82-83	0.18643452221129309
1104	84-85	0.2125515060889569
1104	86-87	0.14847498974010875
1104	88-89	0.2968327503330812
1104	90-91	0.26301144627442596
1104	92-93	0.26969007820672886
1104	94-95	0.28375990809499285
1104	96-97	0.23950419497066378
1104	98-99	0.27094310269374944
1104	100	0.3220612816341557
1105	1	0.14729867626338944
1105	2	0.1651092290499392
1105	3	0.17928918715569608
1105	4	0.1302989223431723
1105	5	0.11840215877503368
1105	6	0.10981721243501141
1105	7	0.1482133888061341
1105	8	0.17386186462247366
1105	9	0.20242728225830575
1105	10-11	0.21341198983429877
1105	12-13	0.17269421485772796
1105	14-15	0.3015833193205495
1105	16-17	0.38314748473093374
1105	18-19	0.27290377633077156
1105	20-21	0.26914045724348057
1105	22-23	0.2625281038097711
1105	24-25	0.26789663166790234
1105	26-27	0.26895775712498704
1105	28-29	0.26527559752258156
1105	30-31	0.2972397128579587
1105	32-33	0.28790951688210953
1105	34-35	0.26620908213616445
1105	36-37	0.2588587238954574
1105	38-39	0.25688054516719205
1105	40-41	0.22680712441442807
1105	42-43	0.2738658342769327
1105	44-45	0.2474569866180616
1105	46-47	0.2695290143856326
1105	48-49	0.3596996586311292
1105	50-51	0.34975072080438707
1105	52-53	0.21731043925977644
1105	54-55	0.21526418678159587
1105	56-57	0.21586876176178293
1105	58-59	0.2481797634478795
1105	60-61	0.13537190765484297
1105	62-63	0.1515030994546791
1105	64-65	0.19891297206689984
1105	66-67	0.15333054757191178
1105	68-69	0.08965673318871836
1105	70-71	0.17212776040446442
1105	72-73	0.18404638090632375
1105	74-75	0.16176451783987034
1105	76-77	0.10361815805704566
1105	78-79	0.12606594433547968
1105	80-81	0.2017371568485018
1105	82-83	0.14696619199534666
1105	84-85	0.13379889882261864
1105	86-87	0.1573153088468331
1105	88-89	0.23333487582193868
1105	90-91	0.207073255490549
1105	92-93	0.21815367566748023
1105	94-95	0.21377064889870923
1105	96-97	0.1952905266602869
1105	98-99	0.1684798028014427
1105	100	0.18469532913174902
1106	1	0.1445244818142868
1106	2	0.10412279916218381
1106	3	0.16599301805980815
1106	4	0.1553787793823247
1106	5	0.15360964746660244
1106	6	0.17113627760505778
1106	7	0.1444074190736231
1106	8	0.13571477130701481
1106	9	0.21461109305855075
1106	10-11	0.2045190429420245
1106	12-13	0.20696470963630276
1106	14-15	0.27581648232942513
1106	16-17	0.36106938678041445
1106	18-19	0.2708451121212434
1106	20-21	0.2350562164438017
1106	22-23	0.21626795414763222
1106	24-25	0.2314557286690686
1106	26-27	0.21963474098604507
1106	28-29	0.24635976851716634
1106	30-31	0.2650735767100798
1106	32-33	0.23925393526597816
1106	34-35	0.23561460790821087
1106	36-37	0.25519850722041326
1106	38-39	0.27963231821683365
1106	40-41	0.26025535694919455
1106	42-43	0.276949418908643
1106	44-45	0.2337191327061916
1106	46-47	0.3053800921764349
1106	48-49	0.343018570229944
1106	50-51	0.23737335441135343
1106	52-53	0.2608089454305471
1106	54-55	0.29930070881208337
1106	56-57	0.234728481424483
1106	58-59	0.29927705780204406
1106	60-61	0.270948525737154
1106	62-63	0.21632167598899343
1106	64-65	0.25886648186753547
1106	66-67	0.2956763480968476
1106	68-69	0.24508652321496527
1106	70-71	0.2826819431263061
1106	72-73	0.2851813281336675
1106	74-75	0.25035015474163913
1106	76-77	0.21039693172097884
1106	78-79	0.16822049283607043
1106	80-81	0.25393476113732305
1106	82-83	0.15038372424530166
1106	84-85	0.1308240984652116
1106	86-87	0.16057840428437942
1106	88-89	0.23080832196032475
1106	90-91	0.19780076428599358
1106	92-93	0.24848989893212092
1106	94-95	0.19557131344330259
1106	96-97	0.2428515782043803
1106	98-99	0.24293336844615965
1106	100	0.2593771574670356
1107	1	0.09702693423857056
1107	2	0.10351664237474978
1107	3	0.12718610892168414
1107	4	0.09222867683004665
1107	5	0.0617778577684831
1107	6	0.08026752398637882
1107	7	0.11622806101149052
1107	8	0.05120648836428643
1107	9	0.10686518501167086
1107	10-11	0.11615971311277917
1107	12-13	0.13272240093643717
1107	14-15	0.19446161457538125
1107	16-17	0.27488325189288787
1107	18-19	0.1969699477106488
1107	20-21	0.1906715058668027
1107	22-23	0.175885642287696
1107	24-25	0.14308064668627907
1107	26-27	0.18155562952206594
1107	28-29	0.1978775440529148
1107	30-31	0.2244331409881113
1107	32-33	0.19515079610668806
1107	34-35	0.18572456650534264
1107	36-37	0.1281467624532695
1107	38-39	0.14674452769900626
1107	40-41	0.16851945865312246
1107	42-43	0.20235522289065955
1107	44-45	0.2292809600962613
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2216	54-55	-0.5189907255922748
2216	56-57	-0.5552062957705317
2216	58-59	-0.5724398594942315
2216	60-61	-0.5857126205802459
2216	62-63	-0.5338715243725503
2216	64-65	-0.4934462950600178
2216	66-67	-0.5551747188469207
2216	68-69	-0.49434596640413986
2216	70-71	-0.4305485982430852
2216	72-73	-0.40934368813244504
2216	74-75	-0.39444967936317
2216	76-77	-0.41866675962749866
2216	78-79	-0.39840644015735194
2216	80-81	-0.42858812196190854
2216	82-83	-0.42101322914113837
2216	84-85	-0.4218814428307738
2216	86-87	-0.3278058187863735
2216	88-89	-0.2935992718148128
2216	90-91	-0.40408314387217104
2216	92-93	-0.38340584814203993
2216	94-95	-0.33127599652526385
2216	96-97	-0.3764203513712161
2216	98-99	-0.40273988209854394
2216	100	-0.46616455757386177
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	25.0
8	104.0
9	274.0
10	340.0
11	222.0
12	218.0
13	279.0
14	506.0
15	932.0
16	1556.0
17	2763.0
18	4739.0
19	7263.0
20	10097.0
21	14756.0
22	21155.0
23	25422.0
24	25224.0
25	23012.0
26	23099.0
27	26476.0
28	32774.0
29	40752.0
30	50910.0
31	64751.0
32	82912.0
33	108424.0
34	155555.0
35	240755.0
36	442117.0
37	964151.0
38	1300582.0
39	436607.0
40	1214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.023360780488375	19.62279769246323	13.504842162840037	23.84899936420836
2	14.754963542172316	21.371997828613782	39.46521219801271	24.407826431201194
3	18.492958202259437	26.776374168209465	30.690768448055078	24.039899181476017
4	12.260592640630676	18.05435054622221	39.02333871814272	30.661718095004396
5	12.66435758838794	38.4519929486735	35.45213772228528	13.431511740653281
6	29.111365464603097	38.26006152175087	18.551883489230285	14.076689524415745
7	25.621793464958103	33.49891458956108	23.118634071425408	17.760657874055404
8	25.173980049411742	34.65676258177099	21.05853252858791	19.110724840229356
9	26.055344050542544	15.811383216831878	21.137197585477047	36.996075147148524
10-11	24.026475657694323	26.11297811854313	28.887077843017607	20.97346838074494
12-13	26.00815919158455	23.91868935168807	28.30551396288923	21.767637493838148
14-15	23.3986234429304	25.04868655783846	26.845624438559557	24.70706556067158
16-17	21.666195655889705	28.02868546873561	28.08991484214367	22.215204033231018
18-19	21.559319122410056	27.833308860267636	29.56622089190395	21.04115112541836
20-21	22.388110905931615	26.786654295068935	28.938504505466796	21.88673029353266
22-23	21.930996509460176	26.630171636456364	28.277484533935315	23.16134732014815
24-25	22.164015468741106	26.88614699002376	28.668254189937336	22.281583351297797
26-27	22.612563358404188	26.22919927045377	28.833657911333272	22.324579459808778
28-29	21.91612070709596	26.525198325874026	29.491196458948377	22.067484508081638
30-31	22.388790726124917	26.885288740457923	28.913291973584133	21.81262855983303
32-33	21.917144611767135	27.128331787493586	28.594101737342196	22.36042186339708
34-35	22.190365981931198	26.76978128968259	28.945704298122827	22.09414843026338
36-37	22.83817224256505	26.359892802019868	28.93558352253808	21.86635143287699
38-39	23.093636806244774	26.093284245752912	29.014256436783764	21.798822511218553
40-41	22.24366347043107	26.386644667962226	29.359134040675546	22.01055782093116
42-43	22.381146218727842	26.67726448345315	28.61075979703968	22.330829500779327
44-45	22.54227949473247	26.880784069554274	28.189719975332984	22.387216460380273
46-47	22.429942242831206	26.631011059458885	28.177130905705788	22.76191579200412
48-49	23.022521495025806	26.679294519110574	28.05069389319246	22.247490092671164
50-51	22.71814414036515	27.042839283828624	27.825996614083913	22.41301996172231
52-53	22.057026957255697	27.26416932951269	27.729491178848605	22.94931253438301
54-55	22.07865201345417	27.085778617365847	27.789874431623534	23.045694937556448
56-57	22.880867453697846	26.900505114405004	27.250557183038275	22.96807024885887
58-59	22.449597061165775	26.66311267177608	27.94298879615682	22.944301470901323
60-61	22.563915463009746	26.747844646412766	27.91488100618488	22.773358884392607
62-63	22.624840311725624	26.63518299020149	27.69319802465255	23.04677867342034
64-65	22.10977019185148	26.90114234966362	28.12832272898697	22.860764729497927
66-67	22.438174215503945	26.58621570200166	27.9894075681118	22.986202514382594
68-69	22.285546814123236	26.897705046552012	27.733827299214536	23.08292084011022
70-71	22.192337222830588	26.801227837122642	27.474224289407694	23.532210650639072
72-73	22.536433000956123	26.48874158923763	27.455288084921097	23.51953732488515
74-75	22.66798723360044	26.848017117895516	27.38202674500852	23.10196890349553
76-77	22.573444419260685	26.350874490834876	27.81827253280367	23.257408557100767
78-79	22.403172430338483	26.41455078445776	27.956969363555253	23.22530742164851
80-81	22.06891995891898	27.134958844767805	27.708460515235426	23.08766068107779
82-83	22.07247677124044	26.826819142626864	27.48572210647972	23.614981979652974
84-85	22.308235735800583	26.751089460123406	27.029426281132235	23.91124852294378
86-87	22.334530545329397	26.626622534909355	27.38806444968942	23.650782470071828
88-89	22.680236284193107	25.92464268560859	27.60350085621146	23.791620173986843
90-91	22.352808643480984	26.250275397755868	27.751723889585904	23.64519206917724
92-93	22.04749899664486	26.87746025782973	27.38711235789249	23.687928387632923
94-95	21.566191715107898	26.917432103403367	27.487814367185297	24.028561814303437
96-97	21.910227727554194	26.524560487888554	27.18610057774355	24.3791112068137
98-99	22.10673045316002	26.554582878037042	26.974355717730653	24.364330951072287
100	21.979705579200374	26.722010069535163	27.15251106506872	24.145773286195745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	523.0
1	320.5
2	374.5
3	696.0
4	499.0
5	318.5
6	618.5
7	1508.0
8	2681.0
9	3296.0
10	2579.5
11	1671.0
12	1638.0
13	1765.0
14	2057.0
15	2427.0
16	2853.5
17	3425.0
18	4011.0
19	4620.5
20	5443.5
21	6531.0
22	7906.5
23	9624.0
24	11676.0
25	14585.5
26	19055.0
27	24311.0
28	30206.5
29	37305.0
30	45711.0
31	53929.5
32	62802.5
33	72679.5
34	81480.0
35	89478.5
36	98747.5
37	109168.0
38	114841.0
39	116707.0
40	118344.5
41	119470.0
42	121055.0
43	125196.5
44	131659.5
45	139736.0
46	150374.0
47	166929.0
48	220374.0
49	228429.5
50	204447.0
51	190755.5
52	153982.0
53	136872.0
54	126940.0
55	114380.5
56	102363.0
57	90824.0
58	80046.5
59	71403.5
60	59835.0
61	47362.5
62	37929.5
63	30158.0
64	22182.0
65	15821.5
66	11654.0
67	8451.5
68	6498.0
69	5048.5
70	4088.5
71	3508.5
72	2957.0
73	2874.5
74	2405.5
75	1896.5
76	1490.0
77	982.5
78	596.0
79	289.0
80	158.5
81	117.0
82	75.5
83	54.0
84	45.5
85	33.5
86	26.0
87	12.5
88	10.0
89	8.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031387120964017708
2	0.003625334126851658
3	1.7031771065746044E-4
4	0.051435948618553044
5	0.002457441253771929
6	0.00486622030449887
7	0.0
8	2.919732182699322E-4
9	7.299330456748305E-5
10-11	4.8662203044988696E-5
12-13	0.0
14-15	4.8662203044988696E-5
16-17	2.5547656598619067E-4
18-19	1.338210583737189E-4
20-21	3.5280097207616805E-4
22-23	0.0
24-25	0.0
26-27	3.406354213149209E-4
28-29	1.2165550761247174E-5
30-31	2.919732182699322E-4
32-33	2.4331101522494347E-4
34-35	2.189799137024491E-4
36-37	1.2165550761247174E-5
38-39	8.029263502423134E-4
40-41	6.569397411073473E-4
42-43	0.0
44-45	5.961119873011115E-4
46-47	0.0
48-49	8.515885532873022E-5
50-51	0.0
52-53	3.6496652283741517E-4
54-55	3.406354213149209E-4
56-57	1.459866091349661E-4
58-59	9.002507563322909E-4
60-61	6.204430888236058E-4
62-63	1.338210583737189E-4
64-65	3.8929762435990957E-4
66-67	0.0012287206268859646
68-69	0.0024452757030106816
70-71	0.0033455264593429722
72-73	0.003102215444118029
74-75	0.0027980766750868497
76-77	0.004245777215675264
78-79	0.0013868727867821778
80-81	9.489129593772795E-4
82-83	6.934363933910889E-4
84-85	4.257942766436511E-4
86-87	1.2165550761247174E-5
88-89	0.0
90-91	3.6496652283741524E-5
92-93	1.338210583737189E-4
94-95	7.299330456748305E-5
96-97	2.676421167474378E-4
98-99	1.2165550761247174E-4
100	0.07598603005474984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4109966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.0344459089343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01282717131136	39.08151595800036
2	15.107294021180556	16.62843111278056
3	5.832941404967142	9.630380947249424
4	2.9355068756523433	6.4621597745357455
5	1.6637224964328758	4.578102286870613
6	0.959562652691457	3.16853993434689
7	0.6414606272629668	2.4711701135670374
8	0.4272084607109571	1.8808944738269007
9	0.2868495433256534	1.4207945108542364
>10	1.0905350828262443	9.339400793079665
>50	0.02844598451614688	1.0679061709515234
>100	0.011864093782211732	1.160838852913287
>500	7.838975496295556E-4	0.2996756249882465
>1k	6.413707224194066E-4	0.5694660482664479
>5k	7.126341360215628E-5	0.22609998403967435
>10k+	2.850536544086251E-4	2.0146234137294003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	16043	0.39034386172537683	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	12802	0.3114867616909726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11396	0.2772772329503456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11127	0.27073216664079464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5765	0.14026880027717992	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8662203044988696E-5
2	0.0	0.0	0.0	0.0	4.8662203044988696E-5
3	0.0	0.0	0.0	0.0	4.8662203044988696E-5
4	0.0	0.0	0.0	0.0	4.8662203044988696E-5
5	0.0	0.0	0.0	0.0	4.8662203044988696E-5
6	0.0	0.0	0.0	0.0	7.299330456748303E-5
7	0.0	0.0	0.0	0.0	7.299330456748303E-5
8	0.0	0.0	0.0	0.0	7.299330456748303E-5
9	0.0	0.0	0.0	0.0	7.299330456748303E-5
10-11	0.0	0.0	0.0	0.0	9.732440608997739E-5
12-13	0.0	0.0	0.0	0.0	2.0681436294120193E-4
14-15	0.0	0.0	0.0	0.0	2.676421167474378E-4
16-17	0.0	0.0	0.0	2.4331101522494348E-5	3.284698705536737E-4
18-19	0.0	0.0	0.0	2.4331101522494348E-5	3.406354213149209E-4
20-21	0.0	0.0	0.0	6.082775380623586E-5	3.8929762435990957E-4
22-23	0.0	0.0	0.0	3.5280097207616805E-4	3.8929762435990957E-4
24-25	0.0	0.0	0.0	7.664296979585719E-4	3.8929762435990957E-4
26-27	0.0	0.0	0.0	0.0017518393096195928	4.136287258824039E-4
28-29	0.0	0.0	0.0	0.005267683479620026	4.136287258824039E-4
30-31	0.0	0.0	0.0	0.013966052273911756	4.136287258824039E-4
32-33	0.0	0.0	0.0	0.03428252204519454	4.3795982740489826E-4
34-35	0.0	0.0	0.0	0.06972077141270755	4.622909289273926E-4
36-37	0.0	0.0	0.0	0.1252565106378009	4.622909289273926E-4
38-39	0.0	0.0	0.0	0.20231310915954048	5.109531319723812E-4
40-41	0.0	0.0	0.0	0.29298295898311566	5.109531319723812E-4
42-43	0.0	0.0	0.0	0.3799544813752717	5.109531319723812E-4
44-45	0.0	0.0	0.0	0.4842254169499212	5.231186827336285E-4
46-47	0.0	0.0	0.0	0.618581759557135	5.352842334948756E-4
48-49	0.0	0.0	0.0	0.7661498902910633	5.352842334948756E-4
50-51	0.0	0.0	0.0	0.9184139236188329	5.352842334948756E-4
52-53	0.0	0.0	0.0	1.0725149551115507	5.596153350173699E-4
54-55	0.0	0.0	0.0	1.2438667375837174	5.596153350173699E-4
56-57	0.0	0.0	0.0	1.4448538017102819	5.596153350173699E-4
58-59	0.0	0.0	0.0	1.689077233242319	5.596153350173699E-4
60-61	0.0	0.0	0.0	1.948519768776676	5.596153350173699E-4
62-63	0.0	0.0	0.0	2.2041301558212405	5.839464365398643E-4
64-65	0.0	0.0	0.0	2.4643877832566012	6.082775380623586E-4
66-67	0.0	0.0	0.0	2.7616408505569146	6.082775380623586E-4
68-69	0.0	0.0	0.0	3.1038456279200366	6.082775380623586E-4
70-71	0.0	0.0	0.0	3.487875568800326	6.082775380623586E-4
72-73	0.0	0.0	0.0	3.8688519564395425	6.204430888236058E-4
74-75	0.0	0.0	0.0	4.245850208979832	6.447741903461002E-4
76-77	0.0	0.0	0.0	4.649430189933445	6.569397411073474E-4
78-79	0.0	0.0	0.0	5.098837800604677	6.569397411073474E-4
80-81	0.0	0.0	0.0	5.58503403677792	6.569397411073474E-4
82-83	0.0	0.0	0.0	6.095391056763	6.569397411073474E-4
84-85	0.0	0.0	0.0	6.613826002453548	6.569397411073474E-4
86-87	0.0	2.4331101522494348E-5	0.0	7.155886447722438	6.812708426298418E-4
88	0.0	2.4331101522494348E-5	0.0	7.57553712123166	7.056019441523361E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	12930	0.0	22.208515	12-13
GTACTTT	14170	0.0	20.18228	14-15
AGTACTT	13730	0.0	19.579533	12-13
GTACCCG	545	0.0	18.981028	1
GTACTAG	1655	0.0	18.183416	1
TACCTGG	4455	0.0	18.048534	2
TAGTACT	2290	0.0	17.453062	4
ACTTTTT	16645	0.0	17.449654	16-17
CTGTGCG	2055	0.0	17.381155	9
CTAGTAC	2155	0.0	17.237253	3
ACCTGGG	4770	0.0	16.659292	3
TACTTTT	16280	0.0	16.411776	14-15
CATGGGG	7450	0.0	16.346762	4
ACCGTAT	260	7.3351766E-6	16.26864	8
GTATAGA	1480	0.0	16.203243	1
TAACGCC	675	0.0	16.021837	4
TAGGCAT	2005	0.0	15.471514	5
CTAACGC	620	0.0	15.167937	3
GTCTATA	1470	0.0	15.033983	1
TGATCGC	910	0.0	14.977297	9
>>END_MODULE