FastQCFastQC Report
Mon 16 Jan 2017
ERR1146582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684531
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT258370.701229003094288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA179080.48603200787291523No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG84450.22920149131599113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15000.024.7617241
TACCTGG42500.020.6822342
CATGGGG74250.019.6877864
GCATCGT17150.018.0827838
CTAGGCA22700.017.600544
GTACCTG52250.017.5466181
ATAGGAC10200.017.5112173
ACCTGGG49150.017.3096393
TAGTACT20350.017.0922894
TAGGCAT22300.017.0728975
GAGTACG6950.016.9122091
CTCGTCG10300.016.8788979
GTATAAG15700.016.7700161
GTACCCG5050.016.7581541
GTATTAG9050.016.624421
TGGGGGG42500.016.3634916
ATGGGGG49100.016.2740065
CTAGTAC17350.016.2549153
TAGGACT20000.015.7462724
GTATTAC9350.015.5881711
Produced by FastQC (version 0.11.5)