##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146582_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3684531
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168781047031494	31.0	31.0	34.0	28.0	34.0
2	31.31433200046356	33.0	31.0	34.0	28.0	34.0
3	31.34222211727897	33.0	31.0	34.0	28.0	34.0
4	34.809066065667515	37.0	35.0	37.0	32.0	37.0
5	34.84041089625789	37.0	35.0	37.0	32.0	37.0
6	34.95883790908531	37.0	35.0	37.0	32.0	37.0
7	34.82379059912917	37.0	35.0	37.0	32.0	37.0
8	34.85603215171754	37.0	35.0	37.0	32.0	37.0
9	36.432677048992126	39.0	37.0	39.0	32.0	39.0
10-11	36.449256771078865	39.0	37.0	39.0	32.0	39.0
12-13	36.442019486333535	39.0	37.0	39.0	32.0	39.0
14-15	37.74692314978487	40.0	38.0	41.0	32.0	41.0
16-17	37.75755068962644	40.0	38.0	41.0	32.0	41.0
18-19	37.76023257776906	40.0	38.0	41.0	32.0	41.0
20-21	37.83920192284988	40.0	38.0	41.0	32.0	41.0
22-23	37.84298435811776	40.0	38.0	41.0	32.0	41.0
24-25	37.847501486620686	40.0	38.0	41.0	32.0	41.0
26-27	37.88126942072139	40.0	38.0	41.0	32.0	41.0
28-29	37.86112479444466	40.0	38.0	41.0	32.0	41.0
30-31	37.83934793872002	40.0	38.0	41.0	31.0	41.0
32-33	37.77648607109019	40.0	38.0	41.0	31.0	41.0
34-35	37.72271816955808	40.0	38.0	41.0	31.0	41.0
36-37	37.65155605964504	40.0	38.0	41.0	31.0	41.0
38-39	37.578502392841855	40.0	37.5	41.0	30.0	41.0
40-41	37.53389101625146	40.0	37.0	41.0	30.0	41.0
42-43	37.37067892765728	40.0	37.0	41.0	30.0	41.0
44-45	37.23374196064574	40.0	37.0	41.0	30.0	41.0
46-47	37.08877927747113	40.0	36.0	41.0	30.0	41.0
48-49	36.96389581197715	40.0	36.0	41.0	30.0	41.0
50-51	36.82238621414774	39.5	36.0	41.0	30.0	41.0
52-53	36.725386623155025	39.0	35.0	41.0	30.0	41.0
54-55	36.592107787938275	39.0	35.0	41.0	29.0	41.0
56-57	36.353705261266626	39.0	35.0	41.0	29.0	41.0
58-59	36.15178173287184	39.0	35.0	40.5	29.0	41.0
60-61	35.887388517018856	38.0	35.0	40.0	28.0	41.0
62-63	35.63788674867982	38.0	34.0	40.0	28.0	41.0
64-65	35.36314160472527	37.0	34.0	40.0	28.0	41.0
66-67	35.088423601267024	36.5	34.0	39.5	28.0	41.0
68-69	34.80174627381341	36.0	34.0	39.0	27.5	41.0
70-71	34.456746598142345	35.5	34.0	39.0	27.0	41.0
72-73	34.11389400713415	35.0	33.0	38.5	26.5	40.0
74-75	33.803389902269785	35.0	33.0	37.0	26.0	39.5
76-77	33.5081753960002	35.0	33.0	37.0	26.0	39.0
78-79	33.19855919247253	35.0	33.0	36.5	26.0	39.0
80-81	32.95598082903903	35.0	33.0	36.0	26.0	38.5
82-83	32.70366744098503	35.0	33.0	36.0	25.5	37.0
84-85	32.521589857705095	35.0	32.0	35.0	25.0	37.0
86-87	32.3607320714631	35.0	32.0	35.0	25.0	36.5
88-89	32.18526469176131	35.0	32.0	35.0	25.0	36.0
90-91	32.03683467448096	35.0	32.0	35.0	25.0	36.0
92-93	31.85114048436558	35.0	32.0	35.0	24.0	36.0
94-95	31.737017954252522	35.0	32.0	35.0	24.0	35.0
96-97	31.66707228138398	35.0	32.0	35.0	24.0	35.0
98-99	31.592422346290476	35.0	32.0	35.0	24.0	35.0
100	30.343486864406895	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23407694604324192
1101	2	0.28137900226025536
1101	3	0.22052357154298008
1101	4	0.20554712632510075
1101	5	0.20881403856074598
1101	6	0.28727138982252143
1101	7	0.2956933936579347
1101	8	0.33906764586259186
1101	9	0.4987469987484374
1101	10-11	0.4128023187641716
1101	12-13	0.43892720981207134
1101	14-15	0.5101801347644894
1101	16-17	0.5056680158962337
1101	18-19	0.5106332869715686
1101	20-21	0.4714621053039991
1101	22-23	0.5045631999552569
1101	24-25	0.5001619902022583
1101	26-27	0.4941109836385209
1101	28-29	0.521417081339834
1101	30-31	0.5488528209105894
1101	32-33	0.6063742993965775
1101	34-35	0.5601588519877296
1101	36-37	0.5644042026672977
1101	38-39	0.5648766201361397
1101	40-41	0.4980621092140112
1101	42-43	0.5577044149237267
1101	44-45	0.45921226833547024
1101	46-47	0.45033262153293663
1101	48-49	0.5707293411053769
1101	50-51	0.5122574063045064
1101	52-53	0.44068825816378165
1101	54-55	0.41568697669253396
1101	56-57	0.47793362281635865
1101	58-59	0.4857325576853242
1101	60-61	0.457297872012532
1101	62-63	0.4726877533420293
1101	64-65	0.5541744874451453
1101	66-67	0.5073630397995004
1101	68-69	0.46970971485270496
1101	70-71	0.39985672919292625
1101	72-73	0.43451415077713307
1101	74-75	0.39939069192740817
1101	76-77	0.3643153404349633
1101	78-79	0.35391989149665903
1101	80-81	0.42047688658005455
1101	82-83	0.42330292505833
1101	84-85	0.3119948758758184
1101	86-87	0.4441787045615797
1101	88-89	0.39426650528627505
1101	90-91	0.35681047733649507
1101	92-93	0.32425618707031134
1101	94-95	0.41961543757445696
1101	96-97	0.3797293899760952
1101	98-99	0.31526720644619743
1101	100	0.3179706469827117
1102	1	0.15039520526485717
1102	2	0.18513532631581242
1102	3	0.20034431424281607
1102	4	0.20580694943166833
1102	5	0.22395833773254736
1102	6	0.30159031853691687
1102	7	0.23246421219179325
1102	8	0.2865070788256858
1102	9	0.39628517582563916
1102	10-11	0.40880452800940503
1102	12-13	0.3566557349405173
1102	14-15	0.4634932942644667
1102	16-17	0.44299776375974886
1102	18-19	0.3695683643862253
1102	20-21	0.43303925058514636
1102	22-23	0.4548351662199508
1102	24-25	0.4307815503203969
1102	26-27	0.42903007175210917
1102	28-29	0.4505482140900554
1102	30-31	0.5174434697883399
1102	32-33	0.49157506830134423
1102	34-35	0.48926266263217855
1102	36-37	0.45635136616723315
1102	38-39	0.4304225340241459
1102	40-41	0.47250602340722025
1102	42-43	0.5342438739136526
1102	44-45	0.41101253200391596
1102	46-47	0.44650532071817395
1102	48-49	0.4291158513519804
1102	50-51	0.45255482058089314
1102	52-53	0.44256937502670723
1102	54-55	0.332497479067122
1102	56-57	0.4057273890856621
1102	58-59	0.39135929238605627
1102	60-61	0.4332272587385191
1102	62-63	0.37340940099048936
1102	64-65	0.41629180087664963
1102	66-67	0.4428492336358687
1102	68-69	0.39041776621179025
1102	70-71	0.3150746361415244
1102	72-73	0.406705347230357
1102	74-75	0.4779065713328947
1102	76-77	0.43301742302706003
1102	78-79	0.4369443838418121
1102	80-81	0.3516604154384808
1102	82-83	0.37733570834708274
1102	84-85	0.40798951588582355
1102	86-87	0.37962160803925826
1102	88-89	0.31098532681706814
1102	90-91	0.338227883420295
1102	92-93	0.3911889710471712
1102	94-95	0.4161328000643181
1102	96-97	0.39614040268714135
1102	98-99	0.38596861954550477
1102	100	0.3610615745459391
1103	1	0.08102532640451088
1103	2	0.09379153412386287
1103	3	0.05266609592894156
1103	4	0.03890721672576092
1103	5	0.0584648827223333
1103	6	0.10496533675274122
1103	7	0.13514812285013278
1103	8	0.11497087606176848
1103	9	0.25409100624041514
1103	10-11	0.22393755901634904
1103	12-13	0.2444781067710906
1103	14-15	0.3642996480725458
1103	16-17	0.30291329219879515
1103	18-19	0.30416434786540236
1103	20-21	0.30643645913276885
1103	22-23	0.2886417892844406
1103	24-25	0.35381068663138393
1103	26-27	0.3771247850294088
1103	28-29	0.33606352969750475
1103	30-31	0.3877410528179155
1103	32-33	0.42533424980043577
1103	34-35	0.37582392039742984
1103	36-37	0.3944971834762683
1103	38-39	0.35915245922105044
1103	40-41	0.23001303388569738
1103	42-43	0.2300806694598876
1103	44-45	0.23978012736395016
1103	46-47	0.2348928977996536
1103	48-49	0.22428928529778602
1103	50-51	0.2828909873796235
1103	52-53	0.2942895921947155
1103	54-55	0.17499413588036106
1103	56-57	0.2537172226453066
1103	58-59	0.2610171501536911
1103	60-61	0.303906891533245
1103	62-63	0.32610459500470057
1103	64-65	0.24865249767494646
1103	66-67	0.21444141889458024
1103	68-69	0.2656904808215401
1103	70-71	0.20708914060194417
1103	72-73	0.2601919765742906
1103	74-75	0.2310407787684241
1103	76-77	0.12840413089606528
1103	78-79	0.14765216792583402
1103	80-81	0.15783781552810439
1103	82-83	0.21427826794298
1103	84-85	0.18056941700405815
1103	86-87	0.24358724197728066
1103	88-89	0.1954938662988397
1103	90-91	0.18705504170993947
1103	92-93	0.17676322871816552
1103	94-95	0.1632690553927496
1103	96-97	0.14634218415451627
1103	98-99	0.12428266581965275
1103	100	0.09833848498778153
1104	1	0.12210079332006174
1104	2	0.17456624723018166
1104	3	0.10260905260978603
1104	4	0.11631392028374421
1104	5	0.11427841635110525
1104	6	0.08729620241100378
1104	7	0.12701843686753023
1104	8	0.23933735530038547
1104	9	0.21886337998536476
1104	10-11	0.31094460366367116
1104	12-13	0.28031409754870396
1104	14-15	0.2963579934381002
1104	16-17	0.4049069832166978
1104	18-19	0.38806062942438047
1104	20-21	0.3438122143264337
1104	22-23	0.30182973644080846
1104	24-25	0.29792494840663863
1104	26-27	0.33556330183107264
1104	28-29	0.3346053120153343
1104	30-31	0.36505153235548704
1104	32-33	0.37980511561177366
1104	34-35	0.3716390928397786
1104	36-37	0.34967912104917076
1104	38-39	0.3397497955186495
1104	40-41	0.3460654606129481
1104	42-43	0.34676988106898676
1104	44-45	0.2561186823895909
1104	46-47	0.33459022378713854
1104	48-49	0.2906902187912195
1104	50-51	0.2767624886364146
1104	52-53	0.2549954346101799
1104	54-55	0.2733507525226955
1104	56-57	0.33270843158098273
1104	58-59	0.3034745781438133
1104	60-61	0.25866494366822934
1104	62-63	0.30125265100709697
1104	64-65	0.367823960347593
1104	66-67	0.2285115227476382
1104	68-69	0.16621657825260883
1104	70-71	0.2234832419535593
1104	72-73	0.21182592774934506
1104	74-75	0.19894372543630112
1104	76-77	0.18361222645510367
1104	78-79	0.15233460502172846
1104	80-81	0.16111718732222613
1104	82-83	0.13147034544985559
1104	84-85	0.13422678520449693
1104	86-87	0.16082676178689326
1104	88-89	0.20547295791887876
1104	90-91	0.2238236704956904
1104	92-93	0.24728702775819755
1104	94-95	0.27354899762006823
1104	96-97	0.2671257846729951
1104	98-99	0.2352211162133493
1104	100	0.21216433132919832
1105	1	0.1126752765664385
1105	2	0.11070537159034544
1105	3	0.11213722350627009
1105	4	0.17522939926197267
1105	5	0.1423016641815309
1105	6	0.14258945663398492
1105	7	0.18487537832066891
1105	8	0.17759555446675535
1105	9	0.2269677245886541
1105	10-11	0.2170623906269924
1105	12-13	0.26835155697865787
1105	14-15	0.26249179178137183
1105	16-17	0.21081966186990542
1105	18-19	0.2780823801041308
1105	20-21	0.27768728156823386
1105	22-23	0.3192966054827906
1105	24-25	0.31604240474363365
1105	26-27	0.27003129673569504
1105	28-29	0.20994552050989768
1105	30-31	0.25583019069249957
1105	32-33	0.2875900325185796
1105	34-35	0.25457070851600605
1105	36-37	0.22728238678408985
1105	38-39	0.19258908245898
1105	40-41	0.2465712655714043
1105	42-43	0.25284467327767146
1105	44-45	0.2370120134032092
1105	46-47	0.2604429364228622
1105	48-49	0.27768247240294386
1105	50-51	0.2241269101769845
1105	52-53	0.2515981824082729
1105	54-55	0.25014025087056524
1105	56-57	0.25094807301138644
1105	58-59	0.17536187456031627
1105	60-61	0.2732708284169547
1105	62-63	0.23723379464090755
1105	64-65	0.22992945014450328
1105	66-67	0.17356822582986808
1105	68-69	0.25048796381023664
1105	70-71	0.2000920956734049
1105	72-73	0.1281058082705897
1105	74-75	0.11436071123070235
1105	76-77	0.1508008961040801
1105	78-79	0.21890548337457005
1105	80-81	0.18060947592968546
1105	82-83	0.23066524367227004
1105	84-85	0.1818313107857179
1105	86-87	0.18289906896541197
1105	88-89	0.1377682178971611
1105	90-91	0.12822133398074698
1105	92-93	0.1795566242007638
1105	94-95	0.11937214052996836
1105	96-97	0.10920638417318784
1105	98-99	0.13083551792300696
1105	100	0.3893373828835749
1106	1	0.048643911198443135
1106	2	0.0976214890909759
1106	3	0.09256901064724232
1106	4	0.10294080960113661
1106	5	0.05760907794402215
1106	6	0.08389027114728265
1106	7	0.08161161957615803
1106	8	0.12557505825235893
1106	9	0.24789234318789966
1106	10-11	0.1686291863557443
1106	12-13	0.2565125779856814
1106	14-15	0.3235976246525638
1106	16-17	0.2876074213650526
1106	18-19	0.22609091654684477
1106	20-21	0.2677273298045222
1106	22-23	0.3221772846281681
1106	24-25	0.33230345293195285
1106	26-27	0.29870716352881743
1106	28-29	0.2196726219483125
1106	30-31	0.22044703956363065
1106	32-33	0.25585590770171507
1106	34-35	0.2417872677337911
1106	36-37	0.25641286291125454
1106	38-39	0.2023565434984178
1106	40-41	0.18075566249579822
1106	42-43	0.2496371621505915
1106	44-45	0.2295671481021131
1106	46-47	0.2491018885374956
1106	48-49	0.14457649142312334
1106	50-51	0.16732496254854112
1106	52-53	0.1990015941241765
1106	54-55	0.17092674134406138
1106	56-57	0.07839526092505622
1106	58-59	0.1403569558391382
1106	60-61	0.17373050373928578
1106	62-63	0.1357528093482685
1106	64-65	0.18630272427926542
1106	66-67	0.22796771687475825
1106	68-69	0.19640591331360469
1106	70-71	0.1810316597796131
1106	72-73	0.1992216637199391
1106	74-75	0.1958004953960355
1106	76-77	0.1431655934028626
1106	78-79	0.12189202652761821
1106	80-81	0.11693868774774074
1106	82-83	0.10386638444531826
1106	84-85	0.11279114726032446
1106	86-87	0.07163158730858044
1106	88-89	0.02303228464030127
1106	90-91	0.08652897411381844
1106	92-93	0.17323666075667177
1106	94-95	0.22062685623293277
1106	96-97	0.18702646080257423
1106	98-99	0.12473522650244817
1106	100	0.1705823537120672
1107	1	-0.0651215630609272
1107	2	0.06198373616687647
1107	3	0.04373310182798207
1107	4	-0.019699419204464164
1107	5	0.015120817037335144
1107	6	0.04629187620150077
1107	7	0.052380850345393526
1107	8	-0.013233530495185164
1107	9	0.0958608397302001
1107	10-11	0.07833421495644188
1107	12-13	0.11869049995272718
1107	14-15	0.16373038312605814
1107	16-17	0.13755086027907737
1107	18-19	0.179754367413409
1107	20-21	0.19172694561601134
1107	22-23	0.18790959061296775
1107	24-25	0.2425328988467541
1107	26-27	0.18416840317706829
1107	28-29	0.19352581925046053
1107	30-31	0.22082375774001406
1107	32-33	0.1960174803133441
1107	34-35	0.12103852701834938
1107	36-37	0.16943279802607947
1107	38-39	0.08368946418483603
1107	40-41	0.15491471653831468
1107	42-43	0.19209404766241533
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2216	40-41	-0.35099895863559283
2216	42-43	-0.32904568289296066
2216	44-45	-0.25870787128626915
2216	46-47	-0.3014108296864748
2216	48-49	-0.2625577375885939
2216	50-51	-0.3229266680287637
2216	52-53	-0.2671597563118553
2216	54-55	-0.2769506208310091
2216	56-57	-0.24238953634773708
2216	58-59	-0.2990683807376797
2216	60-61	-0.3388013406403161
2216	62-63	-0.3579811708225762
2216	64-65	-0.2465868009702774
2216	66-67	-0.25824243929241675
2216	68-69	-0.3418883968224762
2216	70-71	-0.3935404287541715
2216	72-73	-0.2532352354771916
2216	74-75	-0.22022081230185364
2216	76-77	-0.3013504640871929
2216	78-79	-0.2710258001876227
2216	80-81	-0.3466714578185943
2216	82-83	-0.29178708788926855
2216	84-85	-0.2292451773710198
2216	86-87	-0.2639574988428137
2216	88-89	-0.2370726637224294
2216	90-91	-0.3189979297142074
2216	92-93	-0.27134095137515857
2216	94-95	-0.3782984133911853
2216	96-97	-0.26357951695797155
2216	98-99	-0.2914099855423409
2216	100	-0.37690937483935727
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1079.0
1	268.0
2	230.0
3	186.0
4	168.0
5	235.0
6	348.0
7	951.0
8	2360.0
9	3184.0
10	3715.0
11	5065.0
12	6965.0
13	8783.0
14	10670.0
15	11652.0
16	11968.0
17	12859.0
18	13862.0
19	15348.0
20	16777.0
21	18716.0
22	21274.0
23	24507.0
24	29706.0
25	35055.0
26	38062.0
27	36835.0
28	40304.0
29	49159.0
30	57600.0
31	67514.0
32	87197.0
33	117006.0
34	173132.0
35	269556.0
36	475477.0
37	864025.0
38	904655.0
39	247491.0
40	587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.85908103199244	19.315915049396235	12.934785886262205	23.89021803234912
2	14.553895622465857	21.466429128159447	39.063035513998386	24.916639735376307
3	19.538446286289332	26.356094135988993	29.601121222697696	24.50433835502398
4	12.387630866445603	17.58597311615334	38.9057142298382	31.120681787562855
5	12.983528436474023	39.030497151069504	34.65164331287007	13.334331099586397
6	30.031768625528176	37.231498069298794	18.517618072938806	14.219115232234222
7	26.08484841241298	32.99685231523786	22.99360901561239	17.92469025673677
8	25.423423565360746	34.31320644728947	20.93266593523428	19.330704052115507
9	25.80405715542833	16.824484164382966	21.00098047470185	36.370478205486855
10-11	24.64229804939971	26.37610763509899	27.929224346127423	21.05236996937388
12-13	26.073342623627134	24.1510068362693	27.327681662497262	22.447968877606307
14-15	23.430735960131397	25.42199324550058	25.716056250193525	25.4312145441745
16-17	22.3910359477173	28.227133109347918	26.868797683422745	22.513033259512035
18-19	22.41831623163783	28.860898740443318	27.099257738203157	21.621527289715694
20-21	23.487789549388253	27.488913417101042	26.099641274897284	22.923655758613425
22-23	22.92303666279493	27.367683000609887	25.708214556883206	24.001065779711972
24-25	23.47483760622938	27.613615884664604	25.61466946988964	23.296877039216376
26-27	23.492425580872048	26.73359226670907	26.54847042946175	23.225511722957133
28-29	22.718316012717857	28.08966927518246	26.367521907281777	22.82449280481791
30-31	23.401448520689485	27.199394471304615	26.147121307563864	23.25203570044203
32-33	22.886862968987835	27.68652113744825	26.254802761653313	23.1718131319106
34-35	23.293602959302795	27.545013307219175	26.524727522887805	22.636656210590225
36-37	23.580521495828506	26.768579859354364	26.86364300048557	22.787255644331562
38-39	23.542200912745844	27.242353037053135	26.5079186134956	22.707527436705423
40-41	23.002006082082303	27.574066805113944	26.511726140594167	22.912200972209583
42-43	23.24268502488242	27.264987518720563	25.848459419746145	23.643868036650872
44-45	23.53654168707977	26.939512060358773	26.149917100271118	23.37402915229034
46-47	23.5434787008076	27.34525133928505	26.68224700812759	22.42902295177976
48-49	23.601222590596777	27.08055558272182	26.68762272360281	22.630599103078588
50-51	22.155182483734702	27.48751069638564	27.218423590454073	23.138883229425584
52-53	22.634419615010966	27.918632652129784	25.706043245522338	23.740904487336916
54-55	22.5625726236325	27.26178599636398	26.026788655127476	24.148852724876043
56-57	23.557887044434874	26.42046125990049	26.16520488290562	23.856446812759017
58-59	23.851295437293967	26.499099934199837	26.44576974507463	23.20383488343156
60-61	23.341997071879145	27.116510032033283	26.983185174982253	22.558307721105315
62-63	22.441426145548938	27.228303178794977	26.757921993173035	23.572348682483053
64-65	21.87992551022224	28.04617018678275	26.49778640277648	23.576117900218527
66-67	23.304290346370866	26.682227140075643	25.96832634936867	24.04515616418482
68-69	23.150801046508786	26.613829564688206	26.132400205389573	24.102969183413432
70-71	23.269654074807825	26.76067951590143	26.203875418462275	23.76579099082847
72-73	23.470062235861636	26.15153188025764	27.06940760196868	23.308998281912043
74-75	22.992103265181868	27.555163782091906	26.259675982697306	23.193056970028923
76-77	22.780115211570546	26.689842828727013	26.61574195890098	23.914300000801457
78-79	23.09839355356515	26.82775870753959	26.66476658626193	23.40908115263333
80-81	23.08947498258491	26.68195535868565	26.743954165439547	23.484615493289894
82-83	22.8384795937802	26.362771625800253	27.157064573439595	23.641684206979953
84-85	22.850924667099196	27.089140690460123	26.42179045371013	23.638144188730546
86-87	22.670176014361953	27.023181690246457	26.47694708484703	23.82969521054456
88-89	22.782728705821146	26.578077467213735	26.23038580109608	24.408808025869043
90-91	23.27058788051427	26.15342024592588	26.268937842594774	24.307054030965073
92-93	23.345436398327802	26.6589849263391	26.515967637805083	23.479611037528013
94-95	22.639709799483356	26.564469127075736	26.99748522225099	23.798335851189922
96-97	22.49676082361671	26.472835402299662	27.263603284393778	23.76680048968985
98-99	23.06529669913174	26.790318757221094	26.210905899749292	23.933478643897875
100	22.89528614618454	25.92818212884142	26.056519666632568	25.12001205834148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3522.0
1	2388.0
2	1225.5
3	1172.0
4	1009.0
5	790.5
6	658.5
7	664.5
8	732.0
9	749.5
10	662.0
11	556.5
12	560.0
13	577.5
14	613.0
15	710.5
16	839.0
17	983.5
18	1204.0
19	1502.5
20	1995.5
21	2879.5
22	4070.0
23	5734.0
24	7984.0
25	11169.5
26	15230.5
27	19784.0
28	24622.5
29	30028.0
30	35765.0
31	41349.0
32	47702.5
33	54346.5
34	60847.5
35	67226.5
36	74622.5
37	83696.5
38	89268.0
39	91943.5
40	94770.0
41	96792.5
42	99561.5
43	105265.5
44	114758.5
45	125582.5
46	136095.5
47	161220.5
48	205640.5
49	213263.5
50	202027.0
51	186223.0
52	150863.0
53	134051.5
54	125575.5
55	114082.5
56	102899.5
57	90651.0
58	80374.5
59	73210.0
60	63135.5
61	51117.5
62	40613.0
63	31440.0
64	22919.0
65	16186.5
66	11755.5
67	8827.5
68	6792.0
69	5497.0
70	4675.0
71	3769.0
72	3133.5
73	2941.5
74	2413.0
75	1830.0
76	1446.0
77	1047.0
78	694.0
79	437.5
80	304.0
81	229.5
82	180.0
83	139.0
84	98.0
85	69.5
86	55.5
87	43.5
88	36.0
89	27.5
90	18.0
91	16.5
92	12.0
93	11.5
94	17.5
95	14.0
96	8.0
97	7.5
98	8.5
99	9.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09173487751901124
2	0.06299309192947487
3	0.07697044752778576
4	0.07911454673607035
5	0.08071583601820692
6	0.08755524108767167
7	0.0839998360714023
8	0.08603537329445729
9	0.07167805074784281
10-11	0.07787965415408366
12-13	0.06926254657648423
14-15	0.07699758802409316
16-17	0.07850388556915386
18-19	0.07812391862085025
20-21	0.07064671188816161
22-23	0.07380857970797368
24-25	0.07644120784979147
26-27	0.0714202160329225
28-29	0.07967092691037203
30-31	0.0846783484790873
32-33	0.08645605098722199
34-35	0.08843730721766217
36-37	0.08938722458842116
38-39	0.09040499319994864
40-41	0.08755524108767167
42-43	0.09276621637869244
44-45	0.08892583615119536
46-47	0.08637462949829978
48-49	0.09485603459436222
50-51	0.09176201801531864
52-53	0.09757008422510219
54-55	0.08979433203303215
56-57	0.0956973899798916
58-59	0.10012129087799777
60-61	0.09965990244077197
62-63	0.10005343963722926
64-65	0.10198041487505466
66-67	0.10415165457964663
68-69	0.10098978675983457
70-71	0.10294390249396734
72-73	0.10028413385584217
74-75	0.10401595209810963
76-77	0.10143760494890665
78-79	0.1081548777849881
80-81	0.10354099341273015
82-83	0.10753064636991791
84-85	0.10462661326502613
86-87	0.10028413385584217
88-89	0.09997201814830707
90-91	0.10059624956337727
92-93	0.09519529079820471
94-95	0.09472033211282521
96-97	0.09296977010099793
98-99	0.10607862981747201
100	0.24608288001919373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3684531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.447961883517145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74938793948648	43.24802841275795
2	14.709198779709688	17.488637767865168
3	5.581831511971654	9.954855208917172
4	2.698594703699732	6.4170382033841165
5	1.453983582820118	4.321818030537504
6	0.852878433490686	3.0421130763256867
7	0.536312442906071	2.231787716447525
8	0.349515135205603	1.6622369948332025
9	0.2423462946471188	1.2966293958114181
>10	0.8127218163090144	7.1971839395124055
>50	0.010412571633344664	0.405599326134442
>100	0.0022686217624979014	0.2234592624363815
>500	6.85207947354754E-5	0.025703290256012545
>1k	2.740831789417822E-4	0.35899064504935
>5k	6.852079473544554E-5	0.3440004535846773
>10k+	1.370415894708911E-4	1.7819182761470347
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	25837	0.701229003094288	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	17908	0.48603200787291523	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	8445	0.22920149131599113	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.142148892219932E-5
2	0.0	0.0	0.0	8.142148892219932E-5	8.142148892219932E-5
3	0.0	0.0	0.0	8.142148892219932E-5	8.142148892219932E-5
4	0.0	0.0	0.0	2.171239704591982E-4	8.142148892219932E-5
5	0.0	0.0	0.0	2.171239704591982E-4	8.142148892219932E-5
6	1.8998347415179843E-4	0.0	0.0	2.171239704591982E-4	1.3570248153699888E-4
7	1.8998347415179843E-4	0.0	0.0	2.44264466766598E-4	1.3570248153699888E-4
8	1.8998347415179843E-4	0.0	0.0	2.44264466766598E-4	1.3570248153699888E-4
9	1.8998347415179843E-4	0.0	0.0	2.7140496307399777E-4	1.3570248153699888E-4
10-11	2.171239704591982E-4	0.0	0.0	4.2067769276469646E-4	1.3570248153699888E-4
12-13	2.44264466766598E-4	0.0	0.0	4.478181890720963E-4	3.121157075350974E-4
14-15	2.44264466766598E-4	0.0	0.0	4.88528933533196E-4	3.528264519961971E-4
16-17	3.3925620384249715E-4	0.0	0.0	4.88528933533196E-4	4.0710744461099665E-4
18-19	3.7996694830359686E-4	0.0	0.0	5.156694298405957E-4	5.020991816868958E-4
20-21	3.7996694830359686E-4	0.0	0.0	6.378016632238948E-4	5.97090918762795E-4
22-23	4.0710744461099665E-4	0.0	0.0	8.549256336830928E-4	5.97090918762795E-4
24-25	4.342479409183964E-4	0.0	0.0	0.0012620330782940896	5.97090918762795E-4
26-27	4.342479409183964E-4	0.0	0.0	0.0021305289601308824	6.513719113775946E-4
28-29	4.342479409183964E-4	0.0	0.0	0.00483100834271716	6.513719113775946E-4
30-31	4.342479409183964E-4	0.0	0.0	0.013393834927701789	6.513719113775946E-4
32-33	4.342479409183964E-4	0.0	0.0	0.034984099740238306	6.785124076849944E-4
34-35	4.342479409183964E-4	0.0	0.0	0.06881472838741212	6.785124076849944E-4
36-37	4.342479409183964E-4	0.0	0.0	0.11875324159302772	6.785124076849944E-4
38-39	4.342479409183964E-4	0.0	0.0	0.19645648252111325	6.920826558386942E-4
40-41	4.478181890720963E-4	0.0	0.0	0.28667149224691013	7.056529039923942E-4
42-43	4.6138843722579617E-4	0.0	0.0	0.37191979114845286	7.056529039923942E-4
44-45	5.156694298405957E-4	0.0	0.0	0.47626142920225123	7.32793400299794E-4
46-47	5.428099261479955E-4	0.0	0.0	0.6097791007865044	7.870743929145935E-4
48-49	5.563801743016955E-4	0.0	0.0	0.7496476485066892	8.277851373756931E-4
50-51	6.649421595312945E-4	0.0	0.0	0.8996803120939951	8.549256336830928E-4
52-53	6.785124076849944E-4	0.0	0.0	1.056864496458301	9.77057867066392E-4
54-55	6.785124076849944E-4	0.0	0.0	1.2256376727458664	0.0010313388596811914
56-57	6.785124076849944E-4	0.0	0.0	1.4331267670159378	0.0010313388596811914
58-59	6.785124076849944E-4	0.0	0.0	1.6650830187071297	0.0010313388596811914
60-61	7.735041447608935E-4	0.0	0.0	1.9047471713496236	0.001085619852295991
62-63	9.499173707589921E-4	0.0	0.0	2.1516442662580393	0.0012213223338329898
64-65	9.499173707589921E-4	0.0	0.0	2.4114873779050847	0.0012213223338329898
66-67	0.0010041983633737918	0.0	0.0	2.710982754657241	0.0012484628301403897
68-69	0.0010041983633737918	0.0	0.0	3.0547578511349203	0.0012756033264477895
70-71	0.0010720496041422911	0.0	0.0	3.4459473946616272	0.0012891735746014894
72-73	0.0013841653116773885	0.0	0.0	3.818532127969611	0.001316314070908889
74-75	0.0013841653116773885	0.0	0.0	4.189746266214072	0.001343454567216289
76-77	0.0013841653116773885	0.0	0.0	4.589919314018528	0.0013705950635236885
78-79	0.0013841653116773885	0.0	0.0	5.057672197628409	0.0014113058079847883
80-81	0.0013841653116773885	0.0	0.0	5.559839230556074	0.0014113058079847883
82-83	0.0013841653116773885	0.0	0.0	6.076879255460193	0.0014113058079847883
84-85	0.0013841653116773885	0.0	0.0	6.578666321439554	0.0014113058079847883
86-87	0.0013841653116773885	0.0	0.0	7.0945393050024546	0.001452016552445888
88	0.0014384463042921881	0.0	0.0	7.516207625882371	0.0014927272969069876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1500	0.0	24.761724	1
TACCTGG	4250	0.0	20.682234	2
CATGGGG	7425	0.0	19.687786	4
GCATCGT	1715	0.0	18.082783	8
CTAGGCA	2270	0.0	17.60054	4
GTACCTG	5225	0.0	17.546618	1
ATAGGAC	1020	0.0	17.511217	3
ACCTGGG	4915	0.0	17.309639	3
TAGTACT	2035	0.0	17.092289	4
TAGGCAT	2230	0.0	17.072897	5
GAGTACG	695	0.0	16.912209	1
CTCGTCG	1030	0.0	16.878897	9
GTATAAG	1570	0.0	16.770016	1
GTACCCG	505	0.0	16.758154	1
GTATTAG	905	0.0	16.62442	1
TGGGGGG	4250	0.0	16.363491	6
ATGGGGG	4910	0.0	16.274006	5
CTAGTAC	1735	0.0	16.254915	3
TAGGACT	2000	0.0	15.746272	4
GTATTAC	935	0.0	15.588171	1
>>END_MODULE