FastQCFastQC Report
Mon 16 Jan 2017
ERR1146582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684531
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT276000.7490776980842339No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA173020.4695848671106309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118670.32207626967991315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113330.3075832446517616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60900.16528562251206463No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA48390.1313328616315075No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG46550.12633901031094594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG19650.022.2433439
CTAGTAC20950.021.9949073
TAACGCA3051.0913936E-1121.5828274
GTACTAG14300.021.0452231
TAGTACT21050.020.9969334
TACCTGG41150.020.4535732
GAGTACT131550.020.31071312-13
GTACCCG5850.020.0952641
CATGGGG72450.019.7943654
GTATCGT1200.005065847719.5835786
ATAACGC4600.019.4211923
GTACTTT134550.019.35136614-15
TGATCGC7250.018.7992139
AGTACTT139050.018.58990912-13
CGATAAT9800.018.46401466-67
CCTAGTA23700.018.4510292
GTATAAG15750.018.2120511
CTATTGA17600.018.158339
GTACACG4450.017.9638121
ATACGGG10950.017.605563
Produced by FastQC (version 0.11.5)