FastQCFastQC Report
Mon 16 Jan 2017
ERR1146581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156515
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.038.185491
GTATCAA53100.033.5600241
ATCAACG53950.031.1939033
CAACGCA54750.030.6500285
TCAACGC58350.028.921534
AACGCAG61400.027.7072126
TATCAAC67550.025.7486522
GTACATG117750.025.5961511
ACGCAGA68050.024.5155937
TACATGG121300.023.9500162
CGCAGAG72500.022.8158368
ACATGGG123700.022.6492963
GTACTAG14300.020.714861
CATGGGG88200.019.1334634
GCAGAGT93500.017.338989
GAGTACA92950.017.3003231
TATACCG5800.017.0191575
AGAGTAC76650.016.64471410-11
AGTACAT91750.016.3954012
GCATCGT13500.016.0123758
Produced by FastQC (version 0.11.5)