FastQCFastQC Report
Mon 16 Jan 2017
ERR1146581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156515
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114240.27484563390243993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105240.2531928791307141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59200.14242700916512993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.058.9170341
GTATCAA120400.044.257491
ATCAACG148500.035.153993
CAACGCA151850.034.4188465
TCAACGC155350.033.7855574
TATCAAC157150.033.518692
AACGCAG159500.032.9436576
ACGCAGA178350.029.2502847
CGCAGAG182100.028.647938
GCAGAGT209550.024.760339
GTACATG116150.024.5378631
GTGGTAT25950.024.1044731
TACATGG117950.023.7247892
ACATGGG116600.023.3941673
GAGTACT145650.022.5065412-13
CAGAGTA201850.021.71176110-11
AGAGTAC190850.021.49795210-11
GTACTTT157850.020.54374114-15
CATGGGG85350.020.332984
AGTACTT156000.019.59736812-13
Produced by FastQC (version 0.11.5)