##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4156515
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23746888920165	33.0	31.0	34.0	30.0	34.0
2	32.38236094420446	34.0	31.0	34.0	30.0	34.0
3	32.48263052100137	34.0	31.0	34.0	30.0	34.0
4	35.99678889646735	37.0	35.0	37.0	35.0	37.0
5	36.036607350147904	37.0	35.0	37.0	35.0	37.0
6	36.10722035166479	37.0	36.0	37.0	35.0	37.0
7	36.07277178116764	37.0	35.0	37.0	35.0	37.0
8	36.07230744987086	37.0	36.0	37.0	35.0	37.0
9	37.847649773909154	39.0	38.0	39.0	35.0	39.0
10-11	37.791575755169895	39.0	38.0	39.0	35.0	39.0
12-13	37.76992829329378	39.0	38.0	39.0	35.0	39.0
14-15	39.12378940049537	40.0	39.0	41.0	36.0	41.0
16-17	39.0602207618642	40.0	38.5	41.0	36.0	41.0
18-19	39.15970578717989	40.0	39.0	41.0	36.0	41.0
20-21	39.22028033099845	40.0	39.0	41.0	36.0	41.0
22-23	39.18617399431976	40.0	39.0	41.0	36.0	41.0
24-25	39.18265217375614	40.5	39.0	41.0	36.0	41.0
26-27	39.11037130865641	40.0	39.0	41.0	35.5	41.0
28-29	39.0492088925458	40.0	39.0	41.0	35.0	41.0
30-31	38.91762305681563	40.0	38.5	41.0	35.0	41.0
32-33	38.822919080046624	40.0	38.0	41.0	35.0	41.0
34-35	38.757137650170876	40.0	38.0	41.0	35.0	41.0
36-37	38.6916971308897	40.0	38.0	41.0	35.0	41.0
38-39	38.61488470509549	40.0	38.0	41.0	35.0	41.0
40-41	38.52473586646506	40.0	38.0	41.0	34.5	41.0
42-43	38.42737076613461	40.0	38.0	41.0	34.0	41.0
44-45	38.29740034620349	40.0	38.0	41.0	34.0	41.0
46-47	38.081718819732394	40.0	38.0	41.0	33.5	41.0
48-49	37.82091138850696	40.0	38.0	41.0	33.0	41.0
50-51	37.7292025891883	40.0	37.0	41.0	33.0	41.0
52-53	37.637378308510854	40.0	37.0	41.0	33.0	41.0
54-55	37.489172419683314	40.0	37.0	41.0	32.5	41.0
56-57	37.35773815323655	40.0	36.0	41.0	32.0	41.0
58-59	37.19584255079074	39.5	36.0	41.0	32.0	41.0
60-61	37.002411756002324	39.0	35.5	41.0	32.0	41.0
62-63	36.7729551078247	39.0	35.0	41.0	31.5	41.0
64-65	36.504241654366695	38.5	35.0	40.5	31.0	41.0
66-67	36.23520713867266	37.5	35.0	40.0	31.0	41.0
68-69	35.94341798357518	37.0	35.0	40.0	31.0	41.0
70-71	35.626003274377695	37.0	35.0	39.0	31.0	41.0
72-73	35.29366524600537	36.0	35.0	39.0	30.5	41.0
74-75	34.96624419736246	36.0	35.0	39.0	30.0	40.5
76-77	34.65998486713028	35.0	34.5	37.5	30.0	39.5
78-79	34.36465199812824	35.0	34.0	37.0	30.0	39.0
80-81	34.09049179420741	35.0	34.0	36.5	30.0	39.0
82-83	33.830883685010164	35.0	34.0	36.0	30.0	38.0
84-85	33.61344190986921	35.0	34.0	36.0	29.0	37.0
86-87	33.41979109903369	35.0	34.0	35.5	29.0	37.0
88-89	33.21789540035342	35.0	34.0	35.0	29.0	36.0
90-91	33.13498339354002	35.0	34.0	35.0	29.0	36.0
92-93	33.038642588803356	35.0	34.0	35.0	29.0	36.0
94-95	32.96242838050627	35.0	34.0	35.0	29.0	36.0
96-97	32.9050431671725	35.0	34.0	35.0	29.0	35.5
98-99	32.83337158653343	35.0	34.0	35.0	29.0	35.0
100	32.1068339702852	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32449521251132296
1101	2	0.349456357618223
1101	3	0.3273551860030466
1101	4	0.31265239813890844
1101	5	0.25728784168200036
1101	6	0.30320594225633357
1101	7	0.2979250886235647
1101	8	0.29137374542642647
1101	9	0.39153751183302177
1101	10-11	0.39235262885853217
1101	12-13	0.38525070671833817
1101	14-15	0.5333684979390725
1101	16-17	0.5759051540379332
1101	18-19	0.48285674088798913
1101	20-21	0.47924631781432936
1101	22-23	0.446086610546395
1101	24-25	0.4768877038135315
1101	26-27	0.5032070327135543
1101	28-29	0.535367648400026
1101	30-31	0.5627544846564945
1101	32-33	0.5590310613280991
1101	34-35	0.5185567300953764
1101	36-37	0.5087298254353882
1101	38-39	0.5367042270591824
1101	40-41	0.5343836038933318
1101	42-43	0.4982712988440099
1101	44-45	0.47127151402003165
1101	46-47	0.491515725351114
1101	48-49	0.5330869696182461
1101	50-51	0.4784028832868401
1101	52-53	0.47814701084670475
1101	54-55	0.4881167915000475
1101	56-57	0.405197969007709
1101	58-59	0.4444111741652179
1101	60-61	0.4206462877504862
1101	62-63	0.4126790070229376
1101	64-65	0.37450318781731085
1101	66-67	0.36458861945757803
1101	68-69	0.3231785276521393
1101	70-71	0.3658377241591637
1101	72-73	0.3546107316865843
1101	74-75	0.3626556238181422
1101	76-77	0.3297735050099888
1101	78-79	0.3035335319732937
1101	80-81	0.3110690160716558
1101	82-83	0.2596932088947028
1101	84-85	0.26515769637825315
1101	86-87	0.2844349762039968
1101	88-89	0.37192575151674134
1101	90-91	0.3061639535340248
1101	92-93	0.3235243355797124
1101	94-95	0.311587705530755
1101	96-97	0.28460349829445875
1101	98-99	0.344009784542628
1101	100	0.4043378521626053
1102	1	0.32213321866679934
1102	2	0.33616398408965864
1102	3	0.33844978310386153
1102	4	0.25364945351535084
1102	5	0.23681045854127802
1102	6	0.2596734290583669
1102	7	0.24780335157775824
1102	8	0.28550073344853644
1102	9	0.3312881010329818
1102	10-11	0.34579430090036567
1102	12-13	0.38491518067817054
1102	14-15	0.5185570355509412
1102	16-17	0.5798224858706647
1102	18-19	0.529554435937925
1102	20-21	0.4813276025120672
1102	22-23	0.48532351647005356
1102	24-25	0.5026918738597956
1102	26-27	0.4976134858111223
1102	28-29	0.48857871626825045
1102	30-31	0.5369421503920719
1102	32-33	0.5384186031417215
1102	34-35	0.5253510702052537
1102	36-37	0.566100952785149
1102	38-39	0.4991440960373197
1102	40-41	0.4898556781151271
1102	42-43	0.5496784259483221
1102	44-45	0.5727217839632885
1102	46-47	0.6098328812472786
1102	48-49	0.7620280375545931
1102	50-51	0.6611317394624621
1102	52-53	0.6354043305503723
1102	54-55	0.6339937042636166
1102	56-57	0.5650444655333118
1102	58-59	0.5610741350543904
1102	60-61	0.5510014977984028
1102	62-63	0.5372953781868972
1102	64-65	0.5818777503590624
1102	66-67	0.5371973907141339
1102	68-69	0.5408425398748946
1102	70-71	0.5181176077402085
1102	72-73	0.48937388865072506
1102	74-75	0.4586530357765355
1102	76-77	0.45261100966786927
1102	78-79	0.472887019511127
1102	80-81	0.47752778286665176
1102	82-83	0.439377029407936
1102	84-85	0.45945442549387394
1102	86-87	0.4356263595668537
1102	88-89	0.48496919570087016
1102	90-91	0.4040526493948846
1102	92-93	0.4239904828901331
1102	94-95	0.4170986243250212
1102	96-97	0.4709391505456608
1102	98-99	0.49625063538541525
1102	100	0.5706022328108631
1103	1	0.24695082491160036
1103	2	0.25615050798594297
1103	3	0.20604774620738908
1103	4	0.1995468118105208
1103	5	0.15245440884873318
1103	6	0.1677822385125154
1103	7	0.1762254003696171
1103	8	0.20311995314832387
1103	9	0.2330467414649391
1103	10-11	0.2821018722078321
1103	12-13	0.22969835820641293
1103	14-15	0.3986804053657451
1103	16-17	0.5065940530119235
1103	18-19	0.3930010532623953
1103	20-21	0.36584307296939755
1103	22-23	0.37282228014726826
1103	24-25	0.35652136051781724
1103	26-27	0.361430384590129
1103	28-29	0.3089756474296479
1103	30-31	0.37693653633741775
1103	32-33	0.38231308145354603
1103	34-35	0.33306160207391144
1103	36-37	0.33630230398401295
1103	38-39	0.3689416386353983
1103	40-41	0.4255501667772137
1103	42-43	0.3950869492576956
1103	44-45	0.46383087708586856
1103	46-47	0.4948323081273358
1103	48-49	0.5088965709562103
1103	50-51	0.39347474426287476
1103	52-53	0.4120801429163805
1103	54-55	0.41985015677124693
1103	56-57	0.4480122155622652
1103	58-59	0.41159002456398497
1103	60-61	0.3860397529988546
1103	62-63	0.44754922806269093
1103	64-65	0.4251994349491355
1103	66-67	0.38110777996266876
1103	68-69	0.3291246465659512
1103	70-71	0.3822674647419646
1103	72-73	0.3730365892482723
1103	74-75	0.3600694057133822
1103	76-77	0.3797954011130926
1103	78-79	0.41468506509055203
1103	80-81	0.31750194279366184
1103	82-83	0.29703635119275873
1103	84-85	0.31935741178244115
1103	86-87	0.332879817526468
1103	88-89	0.4019512513375503
1103	90-91	0.36876929459241126
1103	92-93	0.35063305726487215
1103	94-95	0.3576995832221641
1103	96-97	0.32377467075544075
1103	98-99	0.36458017535174037
1103	100	0.4456045497576113
1104	1	0.26684599330287995
1104	2	0.24048442990219598
1104	3	0.21089407068524224
1104	4	0.15390034380045847
1104	5	0.20024392577350625
1104	6	0.18261284381564735
1104	7	0.16224859475199338
1104	8	0.1978425753002142
1104	9	0.23677463945315935
1104	10-11	0.2665456413670455
1104	12-13	0.22119790010515317
1104	14-15	0.4213848427747209
1104	16-17	0.44807323406190136
1104	18-19	0.3721005171147951
1104	20-21	0.34164664851428483
1104	22-23	0.35214823257182815
1104	24-25	0.36754236751843194
1104	26-27	0.37181458873929074
1104	28-29	0.3602968188728539
1104	30-31	0.3183600515548122
1104	32-33	0.3353719260906445
1104	34-35	0.37993996923232487
1104	36-37	0.3932056593919384
1104	38-39	0.42900939679962136
1104	40-41	0.4150012916766457
1104	42-43	0.39585750711354706
1104	44-45	0.370627304138722
1104	46-47	0.36219799967610555
1104	48-49	0.42175319446948123
1104	50-51	0.3220642734055872
1104	52-53	0.28343810233827327
1104	54-55	0.32658530858839185
1104	56-57	0.2994270626103983
1104	58-59	0.26339146368275834
1104	60-61	0.19037098297253863
1104	62-63	0.2534497064034653
1104	64-65	0.1982652506612581
1104	66-67	0.23053224521027715
1104	68-69	0.24849991035419805
1104	70-71	0.18345728623020818
1104	72-73	0.26211419641106204
1104	74-75	0.2633246008699359
1104	76-77	0.27007450531567656
1104	78-79	0.2671982846553007
1104	80-81	0.24229690340865773
1104	82-83	0.1963923117830646
1104	84-85	0.20554746092966525
1104	86-87	0.23427436081900055
1104	88-89	0.2649853275337932
1104	90-91	0.20214233146188576
1104	92-93	0.22169207689249504
1104	94-95	0.23670029017823424
1104	96-97	0.24070146576087836
1104	98-99	0.2545650927657661
1104	100	0.26946957695608376
1105	1	0.1589525831443197
1105	2	0.21092366665186546
1105	3	0.17329638290324567
1105	4	0.13129448485425144
1105	5	0.11630619438713552
1105	6	0.1317097658713351
1105	7	0.11087596342503048
1105	8	0.1579864415188581
1105	9	0.22528987326418104
1105	10-11	0.19372378215480524
1105	12-13	0.19809934862934853
1105	14-15	0.32396594799990197
1105	16-17	0.39906703595181625
1105	18-19	0.33362250850839104
1105	20-21	0.29774071537326563
1105	22-23	0.30702251834779304
1105	24-25	0.24222029900703745
1105	26-27	0.296555723271517
1105	28-29	0.3139347917193476
1105	30-31	0.3555983484146239
1105	32-33	0.28691862035896065
1105	34-35	0.3330491405161524
1105	36-37	0.32192563957765685
1105	38-39	0.3752080795214212
1105	40-41	0.38628652119780327
1105	42-43	0.35113028380384037
1105	44-45	0.3621756015611055
1105	46-47	0.39792157969858266
1105	48-49	0.4290728054457773
1105	50-51	0.3576281634045273
1105	52-53	0.3370635240329136
1105	54-55	0.37829908598723705
1105	56-57	0.32791855799504077
1105	58-59	0.323398857877379
1105	60-61	0.3261523265717088
1105	62-63	0.316513697619186
1105	64-65	0.315442910448418
1105	66-67	0.28199327297287624
1105	68-69	0.24241782228627784
1105	70-71	0.25193389370824093
1105	72-73	0.28589370769433486
1105	74-75	0.2686342329735396
1105	76-77	0.20983653536769253
1105	78-79	0.24521886848996388
1105	80-81	0.24040143748236176
1105	82-83	0.22663264812348416
1105	84-85	0.2503449853726636
1105	86-87	0.26728979055143043
1105	88-89	0.32162192445754556
1105	90-91	0.30847654106465683
1105	92-93	0.24074899976498898
1105	94-95	0.2536598573875466
1105	96-97	0.21290773393554474
1105	98-99	0.27255572513636395
1105	100	0.26570716797674265
1106	1	0.13863742857223116
1106	2	0.13680256323874573
1106	3	0.1547888268328137
1106	4	0.1264175144437445
1106	5	0.08493620143944014
1106	6	0.1221924042885334
1106	7	0.12601171504471154
1106	8	0.15674982496464196
1106	9	0.15729684821130974
1106	10-11	0.1814998969597852
1106	12-13	0.15703007860575013
1106	14-15	0.3168942387049398
1106	16-17	0.3753830512027392
1106	18-19	0.29569539493441965
1106	20-21	0.23895686735168198
1106	22-23	0.2425303838475159
1106	24-25	0.21179298050953577
1106	26-27	0.24263071176225282
1106	28-29	0.24589520028341383
1106	30-31	0.231686736074181
1106	32-33	0.22414245838488966
1106	34-35	0.2257732047441081
1106	36-37	0.18020687988177997
1106	38-39	0.16443805602911965
1106	40-41	0.14751777200491745
1106	42-43	0.21291829769545956
1106	44-45	0.20865314162758608
1106	46-47	0.23694300851558836
1106	48-49	0.37795700205747806
1106	50-51	0.27530962083325505
1106	52-53	0.24632330564627125
1106	54-55	0.3197899193541147
1106	56-57	0.30468569327098294
1106	58-59	0.2899177463763394
1106	60-61	0.27460560725594263
1106	62-63	0.2781426966154257
1106	64-65	0.27527346960979315
1106	66-67	0.2111929789731164
1106	68-69	0.1344849650883191
1106	70-71	0.1974506228058317
1106	72-73	0.16814599602457747
1106	74-75	0.12823594600848764
1106	76-77	0.13691270161843505
1106	78-79	0.12629907021437958
1106	80-81	0.1564339811211113
1106	82-83	0.12384297849166614
1106	84-85	0.1347392749137839
1106	86-87	0.12808154065499622
1106	88-89	0.21748678247226394
1106	90-91	0.19430693314259884
1106	92-93	0.19426007884304397
1106	94-95	0.19657194812748457
1106	96-97	0.18301938791057637
1106	98-99	0.17829931244489927
1106	100	0.20058944019401537
1107	1	0.0936513207951819
1107	2	0.10075009556472736
1107	3	0.13252933746323947
1107	4	0.08262295065792102
1107	5	0.07738418061975239
1107	6	0.11712819026557497
1107	7	0.10281391265975515
1107	8	0.1116858623479402
1107	9	0.16102364179217687
1107	10-11	0.11110751465034951
1107	12-13	0.15932811423082427
1107	14-15	0.2553994075364088
1107	16-17	0.32689099199088645
1107	18-19	0.21821234255433808
1107	20-21	0.2358915864741249
1107	22-23	0.2078629821554756
1107	24-25	0.23582620181840497
1107	26-27	0.20816965580733182
1107	28-29	0.2482269932803689
1107	30-31	0.23306962252614483
1107	32-33	0.1486684258123674
1107	34-35	0.11184851186524014
1107	36-37	0.13778151712688924
1107	38-39	0.12617346676205443
1107	40-41	0.15157666513618295
1107	42-43	0.16854255969646204
1107	44-45	0.1606348007304348
1107	46-47	0.15267457232735637
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2216	62-63	-0.8572185292919059
2216	64-65	-0.8689510147745452
2216	66-67	-0.8778844437983437
2216	68-69	-0.7495278767460505
2216	70-71	-0.7004824097517002
2216	72-73	-0.6874799362337285
2216	74-75	-0.6503791439814677
2216	76-77	-0.7586195287227753
2216	78-79	-0.7118669586346087
2216	80-81	-0.657567044837208
2216	82-83	-0.6889076311274991
2216	84-85	-0.6885804456780633
2216	86-87	-0.626093753115434
2216	88-89	-0.5260684485684664
2216	90-91	-0.5654102710915794
2216	92-93	-0.6105030308140584
2216	94-95	-0.6241950633577886
2216	96-97	-0.6387328489403146
2216	98-99	-0.6625293428422125
2216	100	-0.7866348275801549
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	17.0
8	133.0
9	259.0
10	304.0
11	210.0
12	216.0
13	309.0
14	529.0
15	972.0
16	1804.0
17	3165.0
18	5252.0
19	7886.0
20	11755.0
21	16780.0
22	24180.0
23	28250.0
24	26996.0
25	23817.0
26	23519.0
27	26218.0
28	32350.0
29	40729.0
30	50848.0
31	64407.0
32	82855.0
33	108147.0
34	154923.0
35	235885.0
36	425321.0
37	957927.0
38	1360213.0
39	439085.0
40	1254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56352057749851	19.46177577808151	13.433567293305352	24.541136351114627
2	15.312052345475132	20.752042760224867	39.89347957576606	24.04242531853394
3	18.00146565408543	25.825272476450174	30.654256243104182	25.51900562636022
4	12.357461537813478	17.763169455072386	38.735679357212405	31.14368964990173
5	12.71244977388172	38.1788029360611	35.65439089531387	13.454356394743305
6	30.058736787350597	38.54298217734405	17.923513938588744	13.474767096716606
7	26.089332048603215	33.08076597822936	23.02195469040771	17.807947282759713
8	25.339751564600256	35.54316102541253	20.643941900058536	18.47314550992868
9	26.05415835140737	15.045512887599347	20.60197064127039	38.29835811972289
10-11	24.255868658964285	26.264309639763674	29.067251773273593	20.412569927998444
12-13	25.215811803878967	24.118197576575568	28.74353875782958	21.922451861715885
14-15	22.468392688152537	25.196026622786956	26.712056459805446	25.62352422925506
16-17	21.73174441733164	28.12872120250138	27.434447591454596	22.705086788712382
18-19	21.56065767186201	27.158201573170572	29.635074902373038	21.646065852594383
20-21	22.443451008677602	26.358361282485664	29.526358253018493	21.671829455818244
22-23	22.267464450386925	26.270661840508215	29.261171919264093	22.200701789840767
24-25	21.968692522461726	26.471924196111406	29.576772849370204	21.982610432056664
26-27	22.014713558128683	26.567358910662115	29.306089140002552	22.111838391206653
28-29	21.871654724168533	26.631195440314237	29.45076938863079	22.04638044688645
30-31	22.226476593699186	26.436584162334302	29.303268680520116	22.033670563446393
32-33	22.15406667932955	26.52479348337203	29.09950437933981	22.221635457958612
34-35	22.37384374206065	26.602894446112508	29.22816053460209	21.79510127722475
36-37	22.28629299861699	26.422739533327743	29.08797743878916	22.202990029266108
38-39	22.192783612303092	26.556267268200717	29.224207286735616	22.026741832760575
40-41	22.288386456656237	26.409794605643413	29.22456003764713	22.077258900053216
42-43	22.311587952888416	26.377626449080537	28.97160241211688	22.33918318591416
44-45	22.346714206750043	26.7633948575632	28.697501559307515	22.192389376379243
46-47	22.485760306410537	26.69470698409605	28.317508778387662	22.502023931105747
48-49	22.605031311721696	26.745595854984604	28.147517009760953	22.501855823532747
50-51	22.51506370120161	26.90336736424625	27.937563078684907	22.644005855867235
52-53	22.444746247147542	27.034924774540148	27.931615624184257	22.58871335412805
54-55	22.431567490371716	26.922649418838525	27.915707527287353	22.730075563502407
56-57	22.53079447199561	26.87457190637624	27.835654872875292	22.75897874875286
58-59	22.401208712158578	26.873106876234516	28.051504995801746	22.674179415805163
60-61	22.443788789503447	26.930039623590922	27.98896231759092	22.637209269314713
62-63	22.342817576677344	26.79618323842995	28.017557045481446	22.843442139411255
64-65	22.195641384115444	26.767441284175664	28.033457452093742	23.00345987961515
66-67	22.314848898223733	26.79308403525107	27.862765760368774	23.029301306156427
68-69	22.305420105872297	26.662231763012546	27.98138302831341	23.050965102801747
70-71	22.229728738094533	26.513290743479494	27.987334679213134	23.269645839212835
72-73	22.216478735559313	26.556786295059737	27.956675959165615	23.270059010215334
74-75	22.21071391408587	26.671225881423126	27.837422491845444	23.280637712645564
76-77	22.198368548245163	26.6192565874054	27.763888085234207	23.418486779115234
78-79	22.133220352765942	26.62683235545958	27.749059477676596	23.490887814097885
80-81	22.052683571371517	26.718078006465092	27.741023886580845	23.488214535582546
82-83	22.231760183260374	26.48570951864319	27.898511977833223	23.384018320263213
84-85	22.247094938512504	26.539220360036474	27.67562240834857	23.538062293102445
86-87	21.912732180685023	26.720834641520604	27.72963648633531	23.63679669145907
88-89	22.11570269805354	26.437448198791536	27.65032725732976	23.79652184582517
90-91	21.97422864758441	26.377184537669724	27.771273262730105	23.877313552015757
92-93	22.073065357812673	26.374641330837168	27.679269021630276	23.873024289719876
94-95	21.796431287475823	26.307592445879514	27.769743532619774	24.126232734024892
96-97	21.888979977757742	26.140932189984245	27.70552886711845	24.264558965139564
98-99	21.690942031556993	26.339515721296575	27.60537291909333	24.364169328053105
100	21.883678785355876	26.106677176812415	27.586291579018585	24.423352458813124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	203.0
1	136.0
2	247.5
3	463.5
4	345.0
5	254.5
6	555.5
7	1470.0
8	2717.5
9	3312.5
10	2666.0
11	1929.0
12	1926.0
13	2129.0
14	2475.5
15	2921.0
16	3408.5
17	3984.0
18	4492.5
19	5045.0
20	5943.5
21	7329.0
22	8951.0
23	10918.0
24	13476.5
25	17073.0
26	22361.0
27	27990.5
28	33753.5
29	40725.0
30	48431.5
31	56594.5
32	66095.0
33	74780.5
34	82162.5
35	90528.5
36	100505.0
37	111066.0
38	117090.0
39	120250.5
40	124549.0
41	128064.0
42	131998.5
43	138173.0
44	144589.0
45	153256.5
46	160458.0
47	163490.5
48	166078.5
49	166348.5
50	163523.0
51	161257.5
52	157086.0
53	151013.5
54	144051.5
55	130615.0
56	117257.5
57	104099.5
58	90622.0
59	79639.5
60	66275.5
61	53218.5
62	42676.5
63	32542.5
64	23781.0
65	17407.5
66	12672.5
67	9081.0
68	6556.5
69	4887.5
70	3684.5
71	2827.5
72	2195.5
73	1684.0
74	1335.5
75	1068.0
76	777.5
77	469.0
78	265.5
79	145.0
80	82.0
81	47.5
82	25.5
83	14.5
84	7.0
85	5.0
86	2.5
87	1.0
88	1.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025502133397810422
2	0.0031035615172807026
3	2.1652754771725832E-4
4	0.05076368063149057
5	0.0014916342176077796
6	0.004234316488693051
7	0.0
8	5.292895610866314E-4
9	0.0
10-11	1.8043962309771528E-4
12-13	0.0
14-15	1.2029308206514351E-5
16-17	3.007327051628588E-4
18-19	1.0826377385862916E-4
20-21	2.0449823951074398E-4
22-23	0.0
24-25	0.0
26-27	2.0449823951074398E-4
28-29	1.2029308206514351E-5
30-31	1.4435169847817222E-4
32-33	2.2855685592377268E-4
34-35	7.217584923908611E-5
36-37	1.2029308206514351E-5
38-39	7.458171088038898E-4
40-41	5.533481774996602E-4
42-43	0.0
44-45	5.413188692931459E-4
46-47	0.0
48-49	1.0826377385862916E-4
50-51	0.0
52-53	2.4058616413028704E-4
54-55	2.646447805433157E-4
56-57	1.4435169847817222E-4
58-59	6.014654103257176E-4
60-61	6.375533349452606E-4
62-63	1.6841031489120092E-4
64-65	2.8870339695634444E-4
66-67	0.0010826377385862917
68-69	0.002225422018205155
70-71	0.0030313856680416165
72-73	0.0027907995039113294
74-75	0.0023457151002702985
76-77	0.0037411148522259632
78-79	0.0011548135878253778
80-81	9.382860401081194E-4
82-83	5.894361021192032E-4
84-85	4.691430200540597E-4
86-87	0.0
88-89	0.0
90-91	1.2029308206514351E-5
92-93	8.420515744560046E-5
94-95	4.8117232826057404E-5
96-97	3.007327051628588E-4
98-99	8.420515744560046E-5
100	0.07381183515517206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4156515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80726157081194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18139103735427	39.86812638062421
2	16.192659654848228	18.397213050801938
3	6.191282762861617	10.551294581062171
4	2.9134930251910376	6.6203024146705385
5	1.5516739142820037	4.407317296061171
6	0.8851001943060945	3.016807095259367
7	0.5618145202761485	2.234060108532518
8	0.36104067767983256	1.6407785771729164
9	0.2524921014681056	1.29090463673964
>10	0.8629490018170975	7.634749197495143
>50	0.02999137887104125	1.1667726808932108
>100	0.014627960579992294	1.4737499733210242
>500	0.0010116616803920863	0.407575323025426
>1k	2.697764481006003E-4	0.22263773285143754
>5k	6.744411202515007E-5	0.22681386654285152
>10k+	1.3488822405030015E-4	0.8408970849463692
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11424	0.27484563390243993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10524	0.2531928791307141	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5920	0.14242700916512993	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.4058616413028702E-5	7.217584923908611E-5
2	0.0	0.0	0.0	2.4058616413028702E-5	9.623446565211481E-5
3	0.0	0.0	0.0	2.4058616413028702E-5	9.623446565211481E-5
4	0.0	0.0	0.0	2.4058616413028702E-5	9.623446565211481E-5
5	0.0	0.0	0.0	2.4058616413028702E-5	1.2029308206514352E-4
6	0.0	0.0	0.0	2.4058616413028702E-5	1.9246893130422962E-4
7	0.0	0.0	0.0	2.4058616413028702E-5	1.9246893130422962E-4
8	0.0	0.0	0.0	2.4058616413028702E-5	1.9246893130422962E-4
9	0.0	0.0	0.0	2.4058616413028702E-5	1.9246893130422962E-4
10-11	0.0	0.0	0.0	2.4058616413028702E-5	1.9246893130422962E-4
12-13	0.0	0.0	0.0	2.4058616413028702E-5	3.969671708149736E-4
14-15	0.0	0.0	0.0	2.4058616413028702E-5	5.292895610866314E-4
16-17	0.0	0.0	0.0	2.4058616413028702E-5	6.976998759778323E-4
18-19	0.0	0.0	0.0	3.6087924619543055E-5	7.337878005973755E-4
20-21	0.0	0.0	0.0	6.014654103257176E-5	8.300222662494903E-4
22-23	0.0	0.0	0.0	1.5638100668468658E-4	8.661101908690333E-4
24-25	0.0	0.0	0.0	3.488499379889162E-4	8.90168807282062E-4
26-27	0.0	0.0	0.0	7.337878005973755E-4	9.26256731901605E-4
28-29	0.0	0.0	0.0	0.0022735392510312125	9.503153483146338E-4
30-31	0.0	0.0	0.0	0.009106186312331364	9.984325811406912E-4
32-33	0.0	0.0	0.0	0.028040317429384953	0.0010585791221732629
34-35	0.0	0.0	0.0	0.056537748570617455	0.0010585791221732629
36-37	0.0	0.0	0.0	0.10500383133466377	0.0010585791221732629
38-39	0.0	0.0	0.0	0.17597674975309846	0.001130754971412349
40-41	0.0	0.0	0.0	0.25261547233680137	0.001130754971412349
42-43	0.0	0.0	0.0	0.33123903077457917	0.001166842896031892
44-45	0.0	0.0	0.0	0.434582817576744	0.001226989437064464
46-47	0.0	0.0	0.0	0.5650526943845986	0.0012871359780970357
48-49	0.0	0.0	0.0	0.706156479647012	0.0014555462929882366
50-51	0.0	0.0	0.0	0.8576415578916472	0.001539751450433837
52-53	1.2029308206514351E-5	0.0	0.0	1.0047359386409047	0.0016961324571185235
54-55	2.4058616413028702E-5	0.0	0.0	1.1704998057266724	0.0018043962309771528
56-57	2.4058616413028702E-5	0.0	0.0	1.370799816673343	0.0018284548473901814
58-59	2.4058616413028702E-5	0.0	0.0	1.5994889949873872	0.0018645427720097244
60-61	2.4058616413028702E-5	0.0	0.0	1.8400751591176743	0.0019006306966292675
62-63	2.4058616413028702E-5	0.0	0.0	2.0858098671603496	0.002020923778694411
64-65	2.4058616413028702E-5	0.0	0.0	2.342358923280681	0.002020923778694411
66-67	2.4058616413028702E-5	0.0	0.0	2.638376139626586	0.0020449823951074397
68-69	2.4058616413028702E-5	0.0	0.0	2.9777830706733885	0.0020690410115204684
70-71	2.4058616413028702E-5	0.0	0.0	3.341044119893709	0.0021532461689660687
72-73	2.4058616413028702E-5	0.0	0.0	3.7124249521534267	0.002441949565922413
74-75	2.4058616413028702E-5	0.0	0.0	4.086740935615533	0.002490066798748471
76-77	2.4058616413028702E-5	0.0	0.0	4.4947269527476745	0.0025141254151614997
78-79	2.4058616413028702E-5	0.0	0.0	4.950421206226851	0.0025863012644005857
80-81	2.4058616413028702E-5	0.0	0.0	5.441108717278778	0.0026945650382592147
82-83	2.4058616413028702E-5	0.0	0.0	5.948925963216781	0.002911092585976473
84-85	2.4058616413028702E-5	2.4058616413028702E-5	0.0	6.458198755447773	0.002911092585976473
86-87	2.4058616413028702E-5	2.4058616413028702E-5	0.0	6.981172929725984	0.003007327051628588
88	2.4058616413028702E-5	2.4058616413028702E-5	0.0	7.388786038303723	0.0031035615172807026
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6410	0.0	58.917034	1
GTATCAA	12040	0.0	44.25749	1
ATCAACG	14850	0.0	35.15399	3
CAACGCA	15185	0.0	34.418846	5
TCAACGC	15535	0.0	33.785557	4
TATCAAC	15715	0.0	33.51869	2
AACGCAG	15950	0.0	32.943657	6
ACGCAGA	17835	0.0	29.250284	7
CGCAGAG	18210	0.0	28.64793	8
GCAGAGT	20955	0.0	24.76033	9
GTACATG	11615	0.0	24.537863	1
GTGGTAT	2595	0.0	24.104473	1
TACATGG	11795	0.0	23.724789	2
ACATGGG	11660	0.0	23.394167	3
GAGTACT	14565	0.0	22.50654	12-13
CAGAGTA	20185	0.0	21.711761	10-11
AGAGTAC	19085	0.0	21.497952	10-11
GTACTTT	15785	0.0	20.543741	14-15
CATGGGG	8535	0.0	20.33298	4
AGTACTT	15600	0.0	19.597368	12-13
>>END_MODULE