FastQCFastQC Report
Mon 16 Jan 2017
ERR1146580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5137976
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.036.6269381
GTATCAA83500.035.3164831
CAACGCA85400.032.7550055
ATCAACG86200.032.3983733
AACGCAG100550.028.5108226
TATCAAC103200.028.3373262
TCAACGC105150.026.7823584
GTACATG175000.026.4724751
ACGCAGA106650.026.219637
TACATGG169200.026.2035122
CGCAGAG109150.025.5757838
ACATGGG182050.023.9921423
GTACTAG19500.023.6367551
CATGGGG138550.022.9727734
GCAGAGT130250.020.8540749
ATGGGGG85650.020.3640945
AGAGTAC106550.019.36273610-11
GGGCTAT33300.019.3347386
CTAACGC9500.018.8063263
GTATATA28600.018.5826991
Produced by FastQC (version 0.11.5)