##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146580_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5137976
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.862651946992358	31.0	31.0	34.0	27.0	34.0
2	31.12308017787549	31.0	31.0	34.0	27.0	34.0
3	31.270521894224498	31.0	31.0	34.0	28.0	34.0
4	34.799302487983596	37.0	35.0	37.0	32.0	37.0
5	34.842234568631696	37.0	35.0	37.0	32.0	37.0
6	34.8967780308822	37.0	35.0	37.0	32.0	37.0
7	34.77817724333473	37.0	35.0	37.0	32.0	37.0
8	34.7812348675821	37.0	35.0	37.0	32.0	37.0
9	36.401475795138005	39.0	37.0	39.0	32.0	39.0
10-11	36.37778660312932	39.0	37.0	39.0	32.0	39.0
12-13	36.32020819092966	39.0	37.0	39.0	32.0	39.0
14-15	37.63140972242766	40.0	38.0	41.0	32.0	41.0
16-17	37.65205617932042	40.0	38.0	41.0	32.0	41.0
18-19	37.67019503399783	40.0	38.0	41.0	31.0	41.0
20-21	37.762597372973325	40.0	38.0	41.0	31.0	41.0
22-23	37.772438894226056	40.0	38.0	41.0	31.0	41.0
24-25	37.79272995825593	40.0	38.0	41.0	31.0	41.0
26-27	37.852906475234604	40.0	38.0	41.0	31.0	41.0
28-29	37.83382765898478	40.0	38.0	41.0	31.0	41.0
30-31	37.797801118572764	40.0	38.0	41.0	31.0	41.0
32-33	37.77018032003264	40.0	38.0	41.0	31.0	41.0
34-35	37.71561895189856	40.0	38.0	41.0	31.0	41.0
36-37	37.63909251425075	40.0	38.0	41.0	30.0	41.0
38-39	37.581511863815635	40.0	37.0	41.0	30.0	41.0
40-41	37.5233982214008	40.0	37.0	41.0	30.0	41.0
42-43	37.36237897958262	40.0	37.0	41.0	30.0	41.0
44-45	37.2327727883509	40.0	37.0	41.0	30.0	41.0
46-47	37.11724110817178	40.0	36.0	41.0	30.0	41.0
48-49	37.00451744033059	40.0	36.0	41.0	30.0	41.0
50-51	36.87302850383108	40.0	35.5	41.0	30.0	41.0
52-53	36.74215196801231	39.0	35.0	41.0	30.0	41.0
54-55	36.61996377561904	39.0	35.0	41.0	30.0	41.0
56-57	36.428503461285146	39.0	35.0	41.0	29.0	41.0
58-59	36.23131559976146	39.0	35.0	40.5	29.0	41.0
60-61	36.01472544830883	38.0	35.0	40.0	29.0	41.0
62-63	35.77813685778213	37.5	35.0	40.0	28.5	41.0
64-65	35.48093850574623	37.0	34.0	40.0	28.0	41.0
66-67	35.24654309401212	37.0	34.0	40.0	28.0	41.0
68-69	34.99082041255156	36.0	34.0	39.0	28.0	41.0
70-71	34.7194175293929	36.0	34.0	39.0	28.0	41.0
72-73	34.38970549881899	35.0	34.0	39.0	27.5	40.5
74-75	34.06481822803376	35.0	33.5	37.5	27.0	40.0
76-77	33.80215448651376	35.0	33.0	37.0	27.0	39.0
78-79	33.49945970942643	35.0	33.0	37.0	26.5	39.0
80-81	33.268663769546606	35.0	33.0	36.0	26.0	39.0
82-83	33.006499641103815	35.0	33.0	36.0	26.0	37.0
84-85	32.80097834244458	35.0	33.0	35.5	26.0	37.0
86-87	32.59229140034908	35.0	33.0	35.0	26.0	37.0
88-89	32.420995057197615	35.0	33.0	35.0	25.0	36.0
90-91	32.28265575783149	35.0	33.0	35.0	25.0	36.0
92-93	32.12918131575546	35.0	33.0	35.0	25.0	36.0
94-95	32.00449165196568	35.0	32.0	35.0	25.0	35.5
96-97	31.91438019562567	35.0	32.0	35.0	25.0	35.0
98-99	31.80765480414856	35.0	32.0	35.0	24.0	35.0
100	30.608319112428706	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24121742338541807
1101	2	0.27965171163044644
1101	3	0.2961672223156384
1101	4	0.3827218798863541
1101	5	0.2723846480992691
1101	6	0.39669138416901717
1101	7	0.4120322213504508
1101	8	0.4204025985397095
1101	9	0.5541099541531693
1101	10-11	0.5295805116380166
1101	12-13	0.4595260443586895
1101	14-15	0.5577942997447423
1101	16-17	0.6044109713828689
1101	18-19	0.5518896843973735
1101	20-21	0.5648116411822812
1101	22-23	0.5652439172079653
1101	24-25	0.5426403660414323
1101	26-27	0.568007504638679
1101	28-29	0.5947055928734883
1101	30-31	0.6275135585224874
1101	32-33	0.6216326146062343
1101	34-35	0.5847742245974672
1101	36-37	0.5894153155885675
1101	38-39	0.6325178250790984
1101	40-41	0.5605854451121246
1101	42-43	0.5442334685719743
1101	44-45	0.5842437154174291
1101	46-47	0.5241855483226061
1101	48-49	0.5082665079307773
1101	50-51	0.5522185273603171
1101	52-53	0.5552876989067528
1101	54-55	0.5398934475272696
1101	56-57	0.5594356978160846
1101	58-59	0.5472877174357578
1101	60-61	0.5071372744315426
1101	62-63	0.5242321518313702
1101	64-65	0.5655138728913514
1101	66-67	0.5456904522080492
1101	68-69	0.5132422635335274
1101	70-71	0.49372292738432577
1101	72-73	0.5400321251494731
1101	74-75	0.5514558913386409
1101	76-77	0.5399068823180357
1101	78-79	0.5335541239239205
1101	80-81	0.517042601364551
1101	82-83	0.5170952188771878
1101	84-85	0.4815712042244584
1101	86-87	0.51892455207458
1101	88-89	0.5080136393201755
1101	90-91	0.4762660006463406
1101	92-93	0.4361341165611705
1101	94-95	0.3825202072162668
1101	96-97	0.47247899899364043
1101	98-99	0.4514502988264866
1101	100	0.4603377172158929
1102	1	0.17969557273780623
1102	2	0.2210438282287548
1102	3	0.2555918137351547
1102	4	0.3056834707180016
1102	5	0.23279370749963135
1102	6	0.30722920770543993
1102	7	0.37371207243555205
1102	8	0.3438914815583871
1102	9	0.4930825418442524
1102	10-11	0.4310130783292152
1102	12-13	0.4132565022072612
1102	14-15	0.5883260030440596
1102	16-17	0.5127431179917252
1102	18-19	0.5151156593262627
1102	20-21	0.5245503975264185
1102	22-23	0.5026684786725042
1102	24-25	0.5108668899436353
1102	26-27	0.46102697984435537
1102	28-29	0.5365790123104759
1102	30-31	0.5569880020332505
1102	32-33	0.5593262575436029
1102	34-35	0.5165884479222314
1102	36-37	0.5798710008805941
1102	38-39	0.49261701954380754
1102	40-41	0.48500305327708304
1102	42-43	0.5279088805223964
1102	44-45	0.4584711517386424
1102	46-47	0.4874962547757704
1102	48-49	0.50893865297666
1102	50-51	0.479857805633749
1102	52-53	0.4753242403579847
1102	54-55	0.41240870916650607
1102	56-57	0.4624302113441985
1102	58-59	0.4506257167402694
1102	60-61	0.46540908229064115
1102	62-63	0.3901781234362218
1102	64-65	0.4453715166834087
1102	66-67	0.42158485897323317
1102	68-69	0.3776638830084167
1102	70-71	0.37375495626285726
1102	72-73	0.39523322754101997
1102	74-75	0.4165642105973646
1102	76-77	0.40574873621238794
1102	78-79	0.3798467302015922
1102	80-81	0.36724436264486116
1102	82-83	0.3473188207194653
1102	84-85	0.33254113558528076
1102	86-87	0.3257597167079922
1102	88-89	0.3506644810611377
1102	90-91	0.2735325423475956
1102	92-93	0.29692414773730036
1102	94-95	0.35746030027721076
1102	96-97	0.35119983320116077
1102	98-99	0.39895265747914266
1102	100	0.3792066485997836
1103	1	0.1305257258837038
1103	2	0.1698526523163686
1103	3	0.1936281010588985
1103	4	0.18423536407678398
1103	5	0.21386252892505553
1103	6	0.25243031814798655
1103	7	0.23267300938635316
1103	8	0.24538458648768824
1103	9	0.3900702376028349
1103	10-11	0.3302257577957093
1103	12-13	0.37820428592072375
1103	14-15	0.4734421259044268
1103	16-17	0.435386623908137
1103	18-19	0.44141592131949636
1103	20-21	0.41761631762062734
1103	22-23	0.4194197887672715
1103	24-25	0.46183919948202146
1103	26-27	0.4045883494389173
1103	28-29	0.3868203382136599
1103	30-31	0.43513821406892106
1103	32-33	0.4339705261222022
1103	34-35	0.4701763284269873
1103	36-37	0.41879273359712954
1103	38-39	0.4260866851237495
1103	40-41	0.35900236150263254
1103	42-43	0.4344671735090415
1103	44-45	0.37565325436309394
1103	46-47	0.35679894548354696
1103	48-49	0.2517923659924932
1103	50-51	0.29553650156139355
1103	52-53	0.2582701699056642
1103	54-55	0.16702814913316644
1103	56-57	0.25686945856058685
1103	58-59	0.2926761661470181
1103	60-61	0.31454054463329584
1103	62-63	0.32052780473564724
1103	64-65	0.2563432438072013
1103	66-67	0.3134886543679798
1103	68-69	0.2714173779958884
1103	70-71	0.3459437645590029
1103	72-73	0.300620330120438
1103	74-75	0.299721550164854
1103	76-77	0.2255133333017625
1103	78-79	0.20493172644950874
1103	80-81	0.17537450461288984
1103	82-83	0.2032350320961953
1103	84-85	0.22733469199254586
1103	86-87	0.1648816092057217
1103	88-89	0.1440763702759753
1103	90-91	0.2064135939955989
1103	92-93	0.1749959966329726
1103	94-95	0.15367674999254177
1103	96-97	0.19847205410320967
1103	98-99	0.1878178942665798
1103	100	0.2991306802541125
1104	1	0.059660705489378074
1104	2	0.05557827013037553
1104	3	0.14585356248977632
1104	4	0.16327910805875945
1104	5	0.0831834728109655
1104	6	0.2173968306995917
1104	7	0.2396684564204179
1104	8	0.21681446781641256
1104	9	0.3153458077183231
1104	10-11	0.26941060226022984
1104	12-13	0.3373448596626929
1104	14-15	0.3890564661089684
1104	16-17	0.3629126805294476
1104	18-19	0.32607398591655823
1104	20-21	0.37192899916114897
1104	22-23	0.3285857578068416
1104	24-25	0.36825755026191587
1104	26-27	0.3194805757353265
1104	28-29	0.36281071573132095
1104	30-31	0.3627745557075883
1104	32-33	0.3594592977631379
1104	34-35	0.3496331600244318
1104	36-37	0.3709329449701855
1104	38-39	0.35405082657892706
1104	40-41	0.3934034870801639
1104	42-43	0.41681422918124156
1104	44-45	0.3814788427192397
1104	46-47	0.3303896062107654
1104	48-49	0.3729247635287436
1104	50-51	0.3641548455083452
1104	52-53	0.3302976804686466
1104	54-55	0.34897244029165364
1104	56-57	0.3758744326817549
1104	58-59	0.31902384357761804
1104	60-61	0.3085506385205079
1104	62-63	0.37739890278424326
1104	64-65	0.41090628932063566
1104	66-67	0.3470452319657227
1104	68-69	0.310877308680503
1104	70-71	0.27036506385687176
1104	72-73	0.35415267574644105
1104	74-75	0.3500934523065169
1104	76-77	0.24573939700943015
1104	78-79	0.3120635789676882
1104	80-81	0.26926242322487326
1104	82-83	0.2699021947893385
1104	84-85	0.3345696676965062
1104	86-87	0.298043700180898
1104	88-89	0.27282724027676863
1104	90-91	0.3158575139878934
1104	92-93	0.2686274372065469
1104	94-95	0.3503698043692083
1104	96-97	0.32549589829367065
1104	98-99	0.37807208465243036
1104	100	0.37271157141636024
1105	1	0.15064471103176658
1105	2	0.1447328145648683
1105	3	0.17198769562983074
1105	4	0.2619536273300369
1105	5	0.17561412201708748
1105	6	0.19082003923551838
1105	7	0.20001164683570494
1105	8	0.28212184522924133
1105	9	0.2679561102148469
1105	10-11	0.371422001650906
1105	12-13	0.353406764895702
1105	14-15	0.39619560010209653
1105	16-17	0.3831945205935341
1105	18-19	0.390551887689206
1105	20-21	0.3085676118783809
1105	22-23	0.3372471924313203
1105	24-25	0.3515690711928059
1105	26-27	0.36933733839752136
1105	28-29	0.3257478842746835
1105	30-31	0.3750297792609558
1105	32-33	0.3962861891716045
1105	34-35	0.3453142938613354
1105	36-37	0.3675568870651773
1105	38-39	0.3701334909729681
1105	40-41	0.3758620746062391
1105	42-43	0.34347350184870606
1105	44-45	0.3219606424981549
1105	46-47	0.25096619142816223
1105	48-49	0.2940103804920611
1105	50-51	0.3286775790341423
1105	52-53	0.33845755909052855
1105	54-55	0.29784548246784226
1105	56-57	0.3040557614469179
1105	58-59	0.30493666991303314
1105	60-61	0.304468655606847
1105	62-63	0.21836957250884126
1105	64-65	0.29650773173386824
1105	66-67	0.2651948992113091
1105	68-69	0.2743909041930479
1105	70-71	0.22048376922860768
1105	72-73	0.21537477602787902
1105	74-75	0.24381839895976043
1105	76-77	0.23399998227768748
1105	78-79	0.250488518801923
1105	80-81	0.14704735594108342
1105	82-83	0.13613776782742093
1105	84-85	0.1533277993140203
1105	86-87	0.11522375129262485
1105	88-89	0.15491919674027343
1105	90-91	0.15047658783691986
1105	92-93	0.22994604619010062
1105	94-95	0.23833414933549335
1105	96-97	0.18885731002979966
1105	98-99	0.197232421739983
1105	100	0.26864157414535583
1106	1	0.07062978947448428
1106	2	0.10824675121097371
1106	3	0.10957997368143779
1106	4	0.08293272831046039
1106	5	0.11650408462649864
1106	6	0.1017909025915884
1106	7	0.1062728703014102
1106	8	0.15325590738098072
1106	9	0.23749265800088892
1106	10-11	0.2155005419425109
1106	12-13	0.2348605594045452
1106	14-15	0.20959670713273937
1106	16-17	0.23969978750547938
1106	18-19	0.2514258918138026
1106	20-21	0.2818935433622869
1106	22-23	0.2961884146187188
1106	24-25	0.2674757776321641
1106	26-27	0.2966296282123153
1106	28-29	0.2736359008600786
1106	30-31	0.26966362824759216
1106	32-33	0.28021444126535044
1106	34-35	0.2658177680785627
1106	36-37	0.305148425668456
1106	38-39	0.2538216688431021
1106	40-41	0.24203331655985494
1106	42-43	0.24847854709614836
1106	44-45	0.23268529505943292
1106	46-47	0.2567423197923091
1106	48-49	0.2556650210657736
1106	50-51	0.2932234710672148
1106	52-53	0.22573877587909408
1106	54-55	0.19369559646187184
1106	56-57	0.22896395936204783
1106	58-59	0.18664724847780434
1106	60-61	0.2544182541289004
1106	62-63	0.1456932676290279
1106	64-65	0.2059334880620085
1106	66-67	0.23301188966001263
1106	68-69	0.14019870304406368
1106	70-71	0.15788434317649802
1106	72-73	0.12196489251054032
1106	74-75	0.09071039564702943
1106	76-77	0.08524145652346249
1106	78-79	0.14888214805511524
1106	80-81	0.12002000111263555
1106	82-83	0.12674596662648696
1106	84-85	0.10084962748866388
1106	86-87	0.09442667359241597
1106	88-89	0.10517930406397369
1106	90-91	0.10715390822576865
1106	92-93	0.11387469775593217
1106	94-95	0.07957181414747794
1106	96-97	0.058764746398150436
1106	98-99	0.09465060815711723
1106	100	0.09443635360616653
1107	1	-0.0014343854001097611
1107	2	0.03344693126868847
1107	3	0.05412252904355341
1107	4	0.016344116464011904
1107	5	0.049034925637791105
1107	6	0.044166621033788545
1107	7	0.06180432872914565
1107	8	0.056877978958887354
1107	9	0.1455092507365734
1107	10-11	0.0918846196866383
1107	12-13	0.11027312541907719
1107	14-15	0.0840109233450903
1107	16-17	0.15808536606179047
1107	18-19	0.1417323128785668
1107	20-21	0.21080796858202433
1107	22-23	0.2797246689545432
1107	24-25	0.29417609912594145
1107	26-27	0.20346604134075363
1107	28-29	0.23177612593884334
1107	30-31	0.26097130567531934
1107	32-33	0.24872462101833293
1107	34-35	0.15694769813561038
1107	36-37	0.20932661092783889
1107	38-39	0.17705857060499142
1107	40-41	0.2178313538115333
1107	42-43	0.2506766688782207
1107	44-45	0.19426471024947034
1107	46-47	0.2525327585819639
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2216	56-57	-0.41535082803576984
2216	58-59	-0.415773209122726
2216	60-61	-0.42067534832215614
2216	62-63	-0.39787917796244443
2216	64-65	-0.403558131165191
2216	66-67	-0.43045644154650375
2216	68-69	-0.45089879983122216
2216	70-71	-0.4586232009935287
2216	72-73	-0.38864405810842584
2216	74-75	-0.35771924912318553
2216	76-77	-0.421448960652711
2216	78-79	-0.390455854926536
2216	80-81	-0.39756343083121237
2216	82-83	-0.38065647910526934
2216	84-85	-0.40804225462994026
2216	86-87	-0.4812308269007559
2216	88-89	-0.49197147225586946
2216	90-91	-0.4058367190745642
2216	92-93	-0.4033745297035338
2216	94-95	-0.4132647602819688
2216	96-97	-0.389487406594224
2216	98-99	-0.3906813430542222
2216	100	-0.47923992005856775
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1477.0
1	400.0
2	250.0
3	250.0
4	205.0
5	307.0
6	425.0
7	923.0
8	2934.0
9	4000.0
10	4466.0
11	5692.0
12	7765.0
13	10309.0
14	13390.0
15	14900.0
16	16306.0
17	18109.0
18	20326.0
19	22438.0
20	25072.0
21	27561.0
22	30850.0
23	35768.0
24	43164.0
25	51859.0
26	55993.0
27	52857.0
28	55553.0
29	67555.0
30	78620.0
31	90884.0
32	114485.0
33	152135.0
34	224180.0
35	343910.0
36	633042.0
37	1220132.0
38	1313825.0
39	375151.0
40	508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61115677386689	19.972071147660365	13.029113529732752	24.387658548739996
2	15.107505815230684	20.728746169270345	40.16526862237068	23.998479393128292
3	17.857144248438146	26.365465891644696	30.2046061112256	25.572783748691556
4	12.303714790597944	17.573671719658673	39.649865520186	30.472747969557386
5	13.280743554901767	38.54814656676972	35.250740968136036	12.920368910192481
6	29.727010951275417	38.511718982847064	18.575636811464225	13.185633254413295
7	26.291325453284607	33.098211015965276	23.19422476059872	17.416238770151395
8	25.105121121582858	35.51430360203226	21.25244613820861	18.128129138176273
9	26.14598803153287	15.789144629527737	20.90718838406217	37.15767895487722
10-11	24.285847710685577	26.668622916975675	28.888561223995886	20.15696814834286
12-13	24.727601803257073	24.644321818323817	28.222069702387014	22.406006676032096
14-15	22.52904268813775	25.81071914648072	25.642566888146025	26.01767127723551
16-17	22.128442636441815	28.494623551192888	26.63800785552687	22.738925956838425
18-19	22.095063461214078	28.3797513129134	27.724073734291494	21.801111491581032
20-21	23.378620729145446	27.53795287233508	26.904285101610824	22.179141296908654
22-23	23.444952266056152	27.38175731770986	26.52017888920063	22.653111527033364
24-25	23.026374863096557	27.481884336727052	26.980218317042564	22.511522483133827
26-27	22.801776779533046	27.667333645544502	26.705244125078842	22.825645449843606
28-29	22.57317531143528	27.86056293255982	26.84301745315323	22.723244302851672
30-31	22.92426446346995	27.60364859817453	26.735394820844988	22.736692117510536
32-33	22.959649091024907	27.83092071725339	26.27047792024332	22.93895227147838
34-35	23.151512270883654	27.730103965975232	26.771859537090727	22.34652422605039
36-37	23.045855010721205	27.496292746640094	26.749572783184238	22.70827945945446
38-39	22.995030188604265	27.553067229984684	26.803105313950187	22.648797267460864
40-41	22.985989526233894	27.25558615144028	27.011312555662826	22.747111766663007
42-43	22.795219303876195	27.29187847765312	26.87932798149187	23.033574236978822
44-45	22.674232640045506	27.735136388163262	26.826265070098238	22.764365901692994
46-47	22.85483984230693	27.46448014942993	26.695844773794676	22.984835234468463
48-49	22.945699843061103	27.252451098806045	26.874597717450765	22.927251340682083
50-51	22.75365668618698	27.41364567392905	26.654826492652344	23.17787114723163
52-53	22.577848949878703	27.562314957002084	26.672793571522845	23.187042521596364
54-55	22.601286004828484	27.451840563608172	26.690604871475976	23.25626856008737
56-57	22.917468433636877	27.332682861068847	26.645196601297027	23.104652103997246
58-59	22.66613156489992	27.239858717548092	26.95704054954432	23.136969168007667
60-61	22.634295713376755	27.522619158478857	26.764968426105558	23.07811670203883
62-63	22.756646973733556	27.176072355700153	26.670147429122487	23.3971332414438
64-65	22.412734006510977	27.285699813164953	26.75242116009172	23.549145020232345
66-67	22.7698627270632	27.087874824631626	26.691675269888542	23.450587178416633
68-69	22.479213594128254	27.09726545620604	26.7631185511878	23.660402398477903
70-71	22.585982411628063	26.88854651718947	26.765534287856802	23.759936783325667
72-73	22.840688735291543	26.939901404127646	26.88118300240592	23.338226858174895
74-75	22.60375002191799	27.21947408514311	26.783043074207498	23.393732818731404
76-77	22.572096305304225	27.069014827020688	26.779599004544387	23.579289863130697
78-79	22.517875810425764	27.151310032246062	26.736566096733906	23.594248060594268
80-81	22.457463250543512	27.083985593040204	26.949292948854204	23.509258207562077
82-83	22.768637908526276	26.732985095985757	27.2600950335117	23.23828196197627
84-85	22.986310814940342	26.89018385670362	26.700987935472327	23.422517392883712
86-87	22.346665788676972	27.03078626704628	27.031107720373786	23.591440223902957
88-89	22.787485926771872	26.605809148031756	26.877211315937178	23.729493609259194
90-91	22.737368750808496	26.629792728751934	26.941989299012036	23.690849221427538
92-93	22.91518876190096	26.56618781172366	26.93150200579457	23.58712142058081
94-95	22.34827760760831	26.584154794550034	27.20811239360242	23.85945520423924
96-97	22.49567924995831	26.54940187416916	27.010999406237772	23.94391946963475
98-99	22.455622685180675	26.745293403653836	26.802134942885697	23.996948968279792
100	22.858667436667588	26.51112518010315	26.469763644185434	24.160443739043828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2690.0
1	1929.0
2	1189.0
3	1196.0
4	1116.0
5	1019.5
6	917.0
7	840.0
8	851.0
9	861.5
10	818.5
11	777.5
12	800.5
13	851.5
14	905.0
15	1023.0
16	1174.5
17	1335.5
18	1603.0
19	2032.0
20	2735.5
21	3793.5
22	5416.0
23	7910.0
24	11212.0
25	15946.5
26	21865.0
27	28761.0
28	35672.5
29	43939.0
30	54298.5
31	65043.5
32	77569.0
33	89508.5
34	100272.0
35	110566.5
36	123263.5
37	138796.5
38	147630.0
39	150430.5
40	150821.5
41	151967.0
42	156467.0
43	165161.5
44	175368.0
45	189499.5
46	201612.0
47	207953.5
48	214593.0
49	213188.5
50	205797.5
51	204278.5
52	202186.0
53	197724.0
54	190456.5
55	172943.5
56	156982.0
57	138036.0
58	118862.5
59	105997.5
60	87450.0
61	67686.0
62	52927.0
63	40597.0
64	29496.0
65	20715.5
66	14913.5
67	10925.0
68	8235.5
69	6443.0
70	5231.5
71	4003.0
72	2967.5
73	2439.5
74	1957.0
75	1449.5
76	1027.5
77	724.5
78	447.0
79	259.0
80	184.5
81	149.0
82	106.0
83	81.5
84	71.0
85	56.5
86	43.0
87	40.5
88	38.0
89	26.5
90	21.0
91	17.5
92	17.5
93	19.5
94	17.5
95	11.0
96	8.5
97	12.0
98	12.0
99	11.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0960105691424016
2	0.06196992745781607
3	0.07812414849738496
4	0.08018721769039015
5	0.08081003103167474
6	0.08777775528729602
7	0.08439120774406109
8	0.08513079858683653
9	0.07105910965718797
10-11	0.0779392507866911
12-13	0.06790611711693476
14-15	0.07665469827029164
16-17	0.07806575974663953
18-19	0.07734563182077923
20-21	0.06971616839004308
22-23	0.07272318905343271
24-25	0.0755745063815012
26-27	0.07066011986042753
28-29	0.07792951932823353
30-31	0.08220162959110747
32-33	0.08441067066097623
34-35	0.08605528714030583
36-37	0.08677541506616614
38-39	0.08917908530518633
40-41	0.08540327942364853
42-43	0.09079450740914322
44-45	0.0869213869430297
46-47	0.0843230875348581
48-49	0.09279918785140297
50-51	0.08963646385269218
52-53	0.0949498401705263
54-55	0.08720359923829928
56-57	0.09308140014667254
58-59	0.09723673290805562
60-61	0.09610788372697732
62-63	0.09733404749263135
64-65	0.09937765376872137
66-67	0.10138233421098113
68-69	0.09836558208913393
70-71	0.10029241086373313
72-73	0.09736324186800405
74-75	0.10132394546023571
76-77	0.09828773042147336
78-79	0.10532357488629765
80-81	0.10079844670352683
82-83	0.10442828070820105
84-85	0.10123636233411756
86-87	0.09805417541849165
88-89	0.09737297332646162
90-91	0.09715888124039505
92-93	0.09193308804867908
94-95	0.09136866345813995
96-97	0.08968512114498005
98-99	0.10288097881344717
100	0.24213814934129704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5137976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.166807856770546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98264358893769	32.26340123486905
2	15.013097339956657	14.462659498173705
3	6.6631273218981155	9.628247203171938
4	3.568672404434667	6.875662320326557
5	2.1588329917327975	5.199204695382539
6	1.4082858512963472	4.069958040407982
7	0.9326671889481606	3.144652089906626
8	0.6590067789739037	2.539380231931622
9	0.47229073115676945	2.0473863210145606
>10	2.062076947424936	16.222954330887518
>50	0.06034806958693189	1.9692655328219775
>100	0.018625099361142466	1.4166693024470625
>500	2.442647189288262E-4	0.06733760237009778
>1k	8.142157297280906E-5	0.09322159628867678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8388750745429714E-5
2	0.0	0.0	0.0	0.0	5.8388750745429714E-5
3	0.0	0.0	0.0	0.0	5.8388750745429714E-5
4	0.0	0.0	0.0	0.0	5.8388750745429714E-5
5	0.0	0.0	0.0	0.0	5.8388750745429714E-5
6	0.0	0.0	0.0	0.0	1.1677750149085943E-4
7	0.0	0.0	0.0	0.0	1.1677750149085943E-4
8	0.0	0.0	0.0	0.0	1.3624041840600267E-4
9	0.0	0.0	0.0	0.0	2.7248083681200534E-4
10-11	0.0	0.0	0.0	1.946291691514324E-5	2.919437537271486E-4
12-13	0.0	0.0	0.0	1.946291691514324E-5	3.1140667064229183E-4
14-15	0.0	0.0	0.0	1.946291691514324E-5	3.795268798452932E-4
16-17	0.0	0.0	0.0	1.946291691514324E-5	3.892583383028648E-4
18-19	9.73145845757162E-6	0.0	0.0	3.892583383028648E-5	3.892583383028648E-4
20-21	1.946291691514324E-5	0.0	0.0	1.2650895994843105E-4	4.2818417213315123E-4
22-23	1.946291691514324E-5	0.0	0.0	2.919437537271486E-4	5.352302151664391E-4
24-25	1.946291691514324E-5	0.0	0.0	5.546931320815823E-4	6.13081882827012E-4
26-27	1.946291691514324E-5	0.0	0.0	0.0011677750149085943	6.228133412845837E-4
28-29	1.946291691514324E-5	0.0	0.0	0.004291573179789085	6.228133412845837E-4
30-31	1.946291691514324E-5	0.0	0.0	0.014869668523169434	6.228133412845837E-4
32-33	1.946291691514324E-5	0.0	0.0	0.04274056554565456	7.882481350633012E-4
34-35	1.946291691514324E-5	0.0	0.0	0.08687272965074185	8.174425104360161E-4
36-37	2.9194375372714857E-5	0.0	0.0	0.1563650744962608	8.95294178096589E-4
38-39	3.892583383028648E-5	0.0	0.0	0.25547024742817015	9.634143872995903E-4
40-41	3.892583383028648E-5	0.0	0.0	0.3637229913101968	0.0010509975134177349
42-43	3.892583383028648E-5	0.0	0.0	0.47877802465406616	0.0010704604303328781
44-45	3.892583383028648E-5	0.0	0.0	0.6233583029582077	0.0011288491810783079
46-47	3.892583383028648E-5	0.0	0.0	0.7976779183086882	0.001148312097993451
48-49	3.892583383028648E-5	0.0	0.0	0.9862443888410535	0.0012067008487388808
50-51	3.892583383028648E-5	0.0	0.0	1.1897875739396215	0.0012067008487388808
52-53	3.892583383028648E-5	0.0	0.0	1.3917347998511476	0.001255358141026739
54-55	3.892583383028648E-5	0.0	0.0	1.6186724110817177	0.0013429412671448835
56-57	3.892583383028648E-5	0.0	0.0	1.8913965343551624	0.0013915985594327416
58-59	3.892583383028648E-5	0.0	0.0	2.194589854059264	0.0014305243932630281
60-61	3.892583383028648E-5	0.0	0.0	2.5145602081442187	0.001459718768635743
62-63	3.892583383028648E-5	0.0	0.0	2.8414788235678796	0.0015181075193811727
64-65	3.892583383028648E-5	0.0	0.0	3.1865368775564544	0.0015181075193811727
66-67	3.892583383028648E-5	0.0	0.0	3.5789482083995723	0.0015473018947538876
68-69	3.892583383028648E-5	0.0	0.0	4.013662189157754	0.001586227728584174
70-71	3.892583383028648E-5	0.0	0.0	4.480519177201295	0.0016738108547023187
72-73	3.892583383028648E-5	0.0	0.0	4.959988135405848	0.0017516625223628916
74-75	3.892583383028648E-5	0.0	0.0	5.439135955481302	0.0017711254392780349
76-77	3.892583383028648E-5	0.0	0.0	5.973159469799002	0.0018489771069386076
78-79	3.892583383028648E-5	0.0	0.0	6.5532809028302195	0.0019073658576840373
80-81	3.892583383028648E-5	0.0	0.0	7.1782059706000965	0.0019560231499718955
82-83	3.892583383028648E-5	0.0	0.0	7.819032241489645	0.002072800651462755
84-85	3.892583383028648E-5	0.0	0.0	8.46179507261225	0.0021019950268354698
86-87	3.892583383028648E-5	0.0	0.0	9.140418328151007	0.002150652319123328
88	3.892583383028648E-5	0.0	0.0	9.671804617226705	0.0021798466944960427
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5085	0.0	36.626938	1
GTATCAA	8350	0.0	35.316483	1
CAACGCA	8540	0.0	32.755005	5
ATCAACG	8620	0.0	32.398373	3
AACGCAG	10055	0.0	28.510822	6
TATCAAC	10320	0.0	28.337326	2
TCAACGC	10515	0.0	26.782358	4
GTACATG	17500	0.0	26.472475	1
ACGCAGA	10665	0.0	26.21963	7
TACATGG	16920	0.0	26.203512	2
CGCAGAG	10915	0.0	25.575783	8
ACATGGG	18205	0.0	23.992142	3
GTACTAG	1950	0.0	23.636755	1
CATGGGG	13855	0.0	22.972773	4
GCAGAGT	13025	0.0	20.854074	9
ATGGGGG	8565	0.0	20.364094	5
AGAGTAC	10655	0.0	19.362736	10-11
GGGCTAT	3330	0.0	19.334738	6
CTAACGC	950	0.0	18.806326	3
GTATATA	2860	0.0	18.582699	1
>>END_MODULE