FastQCFastQC Report
Mon 16 Jan 2017
ERR1146580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5137976
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175260.34110708185480043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160180.3117570031467644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90500.17613939808204632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96550.062.6882631
GTATCAA185000.043.6982571
ATCAACG222150.035.7537963
CAACGCA223250.035.5589335
TATCAAC234850.034.261532
TCAACGC240350.033.2613144
AACGCAG243500.033.2197726
ACGCAGA262500.030.2757747
CGCAGAG267950.029.6424358
GCAGAGT299650.026.2085579
TACATGG168300.025.7349172
GTACATG174200.025.673931
GAGTACT202000.024.25536212-13
GTGGTAT31950.024.1397631
ACATGGG177800.023.7510853
CAGAGTA287750.023.70767610-11
CTAACGC9800.023.0331883
GTACTAG19750.022.8593641
GTACTTT217800.022.3124814-15
CATGGGG137050.022.2003524
Produced by FastQC (version 0.11.5)