##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5137976
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31485121767793	33.0	31.0	34.0	30.0	34.0
2	32.444147072699444	34.0	31.0	34.0	30.0	34.0
3	32.53816892099146	34.0	31.0	34.0	30.0	34.0
4	36.039299521835055	37.0	35.0	37.0	35.0	37.0
5	36.091028646299634	37.0	35.0	37.0	35.0	37.0
6	36.15603770823375	37.0	36.0	37.0	35.0	37.0
7	36.12437952220875	37.0	36.0	37.0	35.0	37.0
8	36.12939745144781	37.0	36.0	37.0	35.0	37.0
9	37.91816252158438	39.0	38.0	39.0	35.0	39.0
10-11	37.86592609619041	39.0	38.0	39.0	35.0	39.0
12-13	37.840635106119606	39.0	38.0	39.0	35.0	39.0
14-15	39.194861556379394	40.0	39.0	41.0	36.0	41.0
16-17	39.132288765070136	40.0	39.0	41.0	36.0	41.0
18-19	39.24241452276149	40.5	39.0	41.0	36.0	41.0
20-21	39.29792918456606	41.0	39.0	41.0	36.0	41.0
22-23	39.26369459491442	41.0	39.0	41.0	36.0	41.0
24-25	39.24992516508446	41.0	39.0	41.0	36.0	41.0
26-27	39.1716488165768	41.0	39.0	41.0	35.5	41.0
28-29	39.11191381586835	40.5	39.0	41.0	35.0	41.0
30-31	38.967320594724455	40.0	38.5	41.0	35.0	41.0
32-33	38.85821294221694	40.0	38.0	41.0	35.0	41.0
34-35	38.78091888712598	40.0	38.0	41.0	35.0	41.0
36-37	38.706092437956116	40.0	38.0	41.0	35.0	41.0
38-39	38.61969577125312	40.0	38.0	41.0	35.0	41.0
40-41	38.51464886173076	40.0	38.0	41.0	35.0	41.0
42-43	38.4001187432561	40.0	38.0	41.0	34.0	41.0
44-45	38.24299899415645	40.0	38.0	41.0	34.0	41.0
46-47	37.990247813535916	40.0	38.0	41.0	33.0	41.0
48-49	37.6893757386177	40.0	37.0	41.0	33.0	41.0
50-51	37.57018610051896	40.0	37.0	41.0	33.0	41.0
52-53	37.45793294869419	40.0	37.0	41.0	33.0	41.0
54-55	37.293276476962916	40.0	36.0	41.0	32.0	41.0
56-57	37.131116027011416	39.5	36.0	41.0	32.0	41.0
58-59	36.940544681407616	39.0	35.0	41.0	32.0	41.0
60-61	36.734474528491376	39.0	35.0	41.0	31.5	41.0
62-63	36.48477250574935	38.5	35.0	41.0	31.0	41.0
64-65	36.20647147826304	37.5	35.0	40.0	31.0	41.0
66-67	35.93250630209249	37.0	35.0	40.0	31.0	41.0
68-69	35.63826553491102	37.0	35.0	40.0	31.0	41.0
70-71	35.33964551410906	36.0	35.0	39.0	30.5	41.0
72-73	35.02517898098395	36.0	35.0	39.0	30.0	41.0
74-75	34.71767832313736	35.0	35.0	38.5	30.0	40.5
76-77	34.431539579009325	35.0	34.0	37.0	30.0	39.5
78-79	34.15826017871629	35.0	34.0	37.0	30.0	39.0
80-81	33.91165110541583	35.0	34.0	36.5	29.0	39.0
82-83	33.66998901902228	35.0	34.0	36.0	29.0	37.5
84-85	33.47039340004702	35.0	34.0	36.0	29.0	37.0
86-87	33.291165431679715	35.0	34.0	35.0	29.0	37.0
88-89	33.10466621486749	35.0	34.0	35.0	29.0	36.0
90-91	33.02608108718297	35.0	34.0	35.0	29.0	36.0
92-93	32.930907423467914	35.0	34.0	35.0	29.0	36.0
94-95	32.859575346400995	35.0	34.0	35.0	29.0	36.0
96-97	32.80729600527523	35.0	34.0	35.0	29.0	35.0
98-99	32.73915409491987	35.0	34.0	35.0	29.0	35.0
100	32.01188911742678	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2951733936460528
1101	2	0.3219578098743341
1101	3	0.37793016440672034
1101	4	0.24201902361476613
1101	5	0.2190464071173679
1101	6	0.22605175973288993
1101	7	0.2802421147943406
1101	8	0.2851113969935426
1101	9	0.31432945417983404
1101	10-11	0.3446981447582047
1101	12-13	0.38390397738213977
1101	14-15	0.5101070271712587
1101	16-17	0.48655372433870525
1101	18-19	0.4403451561685756
1101	20-21	0.4142836920205468
1101	22-23	0.4293169224696598
1101	24-25	0.4667594155882213
1101	26-27	0.45647675995066095
1101	28-29	0.46460934988032676
1101	30-31	0.5592039164865525
1101	32-33	0.5423618655214213
1101	34-35	0.5279671174218734
1101	36-37	0.5562458004156454
1101	38-39	0.5422783207260409
1101	40-41	0.523180094271325
1101	42-43	0.5031680514565835
1101	44-45	0.4883083011258691
1101	46-47	0.5078204371342778
1101	48-49	0.5732829212168937
1101	50-51	0.5076645667483461
1101	52-53	0.5071948122086951
1101	54-55	0.49062421027301184
1101	56-57	0.48267140013888366
1101	58-59	0.48015926045546564
1101	60-61	0.47758708360940716
1101	62-63	0.4681954323384758
1101	64-65	0.46924644007816596
1101	66-67	0.4294300233048034
1101	68-69	0.415995741732786
1101	70-71	0.40562910610398717
1101	72-73	0.40967485588076613
1101	74-75	0.44111015169780643
1101	76-77	0.3871900680983842
1101	78-79	0.3333566247932538
1101	80-81	0.35214227066386883
1101	82-83	0.3889013587064625
1101	84-85	0.35690633968103214
1101	86-87	0.37545977950743037
1101	88-89	0.5063128161895278
1101	90-91	0.40218584215909914
1101	92-93	0.39994507643336874
1101	94-95	0.4425865508104181
1101	96-97	0.3803287413933205
1101	98-99	0.4060639283060965
1101	100	0.5503940336419078
1102	1	0.24822687500046925
1102	2	0.27227862407966086
1102	3	0.27009906314210497
1102	4	0.18488938220848183
1102	5	0.19764248729089928
1102	6	0.20665633335093503
1102	7	0.17082678163759368
1102	8	0.21189418027253026
1102	9	0.28012835649894186
1102	10-11	0.2640558677256166
1102	12-13	0.2563622883247234
1102	14-15	0.4261742620887219
1102	16-17	0.4951344066865673
1102	18-19	0.4429982554037437
1102	20-21	0.36715907411262094
1102	22-23	0.3760737584556466
1102	24-25	0.3646434043014821
1102	26-27	0.35598284703196015
1102	28-29	0.35624591360453195
1102	30-31	0.4094453560358886
1102	32-33	0.37644673063524436
1102	34-35	0.38313784208234836
1102	36-37	0.4173676614991919
1102	38-39	0.4012677617166034
1102	40-41	0.37338182535508935
1102	42-43	0.42320507605264623
1102	44-45	0.42032287857162487
1102	46-47	0.46220673389077405
1102	48-49	0.5335565792791144
1102	50-51	0.5696201304690476
1102	52-53	0.5130545436695542
1102	54-55	0.5261360194800773
1102	56-57	0.5238200298616391
1102	58-59	0.41000629921853715
1102	60-61	0.4183862003240293
1102	62-63	0.39159315196735633
1102	64-65	0.45954706607683704
1102	66-67	0.38190983056867367
1102	68-69	0.3719685955158951
1102	70-71	0.37412616091350515
1102	72-73	0.35646825989094566
1102	74-75	0.36680410771302974
1102	76-77	0.3498911061712917
1102	78-79	0.40237310860771913
1102	80-81	0.3447272356074862
1102	82-83	0.2794858745669586
1102	84-85	0.27942476591888976
1102	86-87	0.29679821509172655
1102	88-89	0.355107840909028
1102	90-91	0.35118072577616033
1102	92-93	0.2939454866233291
1102	94-95	0.28235440696757763
1102	96-97	0.310023529142363
1102	98-99	0.3468846053586887
1102	100	0.38280758000828996
1103	1	0.23469744888434008
1103	2	0.23481849105334618
1103	3	0.22283149127213164
1103	4	0.18260943288579057
1103	5	0.15281875217092278
1103	6	0.1826277701244905
1103	7	0.1896280501434262
1103	8	0.17405698236700573
1103	9	0.21450967803268384
1103	10-11	0.2521454132256906
1103	12-13	0.23228971070612658
1103	14-15	0.3675183709680212
1103	16-17	0.43075175938827925
1103	18-19	0.32675134148207974
1103	20-21	0.2880940845485611
1103	22-23	0.31870508356519167
1103	24-25	0.35012329249540386
1103	26-27	0.3602874012569188
1103	28-29	0.33288714619948934
1103	30-31	0.3083999104236881
1103	32-33	0.312040390604885
1103	34-35	0.33183848713602515
1103	36-37	0.30964795992780836
1103	38-39	0.333220435369995
1103	40-41	0.2998517836850212
1103	42-43	0.34893106545418817
1103	44-45	0.3422012654157811
1103	46-47	0.3522224527995874
1103	48-49	0.38274007421345146
1103	50-51	0.36634176484551517
1103	52-53	0.33647630631626413
1103	54-55	0.3730446502681417
1103	56-57	0.3647583778493342
1103	58-59	0.32228098757388324
1103	60-61	0.2792147016446478
1103	62-63	0.32721033325864113
1103	64-65	0.3111731121828569
1103	66-67	0.25945072610024056
1103	68-69	0.28274006191678325
1103	70-71	0.33539788582992003
1103	72-73	0.31152954645619246
1103	74-75	0.2664835949474522
1103	76-77	0.247154820354055
1103	78-79	0.24286003767596753
1103	80-81	0.2714994857731057
1103	82-83	0.22506250719474963
1103	84-85	0.247428254997466
1103	86-87	0.19490595428646884
1103	88-89	0.26503859623313986
1103	90-91	0.2217617384168591
1103	92-93	0.25667954258118186
1103	94-95	0.2373730789926043
1103	96-97	0.2249403761280533
1103	98-99	0.28470358233432336
1103	100	0.3223196540930999
1104	1	0.21284924380557158
1104	2	0.21035106848187013
1104	3	0.21228028921756703
1104	4	0.12883529232399837
1104	5	0.11218972661727378
1104	6	0.14713917838302137
1104	7	0.16559001397047268
1104	8	0.1554133239963278
1104	9	0.21364510028807615
1104	10-11	0.2271314449583386
1104	12-13	0.21019878513491363
1104	14-15	0.3330758918787282
1104	16-17	0.3611400864762686
1104	18-19	0.32777425231606117
1104	20-21	0.2807398540151169
1104	22-23	0.28339834124083296
1104	24-25	0.31029933049505587
1104	26-27	0.3294168339925889
1104	28-29	0.29207043033724744
1104	30-31	0.3179949854990056
1104	32-33	0.35251802923705355
1104	34-35	0.3380223827342377
1104	36-37	0.3573874711199565
1104	38-39	0.37056727528864997
1104	40-41	0.38347059732950584
1104	42-43	0.40785353851065054
1104	44-45	0.4119518886433937
1104	46-47	0.46826421686312614
1104	48-49	0.5162212126538819
1104	50-51	0.46030115799737814
1104	52-53	0.3632470979265321
1104	54-55	0.3970354560938887
1104	56-57	0.36688924289740044
1104	58-59	0.3369485803723009
1104	60-61	0.33587785497014977
1104	62-63	0.35293945129426874
1104	64-65	0.33120044180400754
1104	66-67	0.3194147469275208
1104	68-69	0.308286710502216
1104	70-71	0.3204424449127927
1104	72-73	0.27252206919851574
1104	74-75	0.2787257261029268
1104	76-77	0.2655632974753033
1104	78-79	0.3082500759654394
1104	80-81	0.32535267606174045
1104	82-83	0.3046119268069276
1104	84-85	0.2144071343877485
1104	86-87	0.2543996755168223
1104	88-89	0.32565717431896957
1104	90-91	0.3207433637090773
1104	92-93	0.3711314159806349
1104	94-95	0.3119677691980982
1104	96-97	0.3119063171349339
1104	98-99	0.2902567245533234
1104	100	0.30110169019229005
1105	1	0.17136685071813673
1105	2	0.19970573227673327
1105	3	0.19532353925714574
1105	4	0.11571202667897751
1105	5	0.12274538883608699
1105	6	0.10940080522493645
1105	7	0.17671994602225993
1105	8	0.15242900028071205
1105	9	0.21949513142769206
1105	10-11	0.18997777798048787
1105	12-13	0.21532147710396998
1105	14-15	0.31501329084265706
1105	16-17	0.37586342318975596
1105	18-19	0.3199716024375405
1105	20-21	0.243684873835619
1105	22-23	0.236048729790852
1105	24-25	0.2677914156391594
1105	26-27	0.26184540675861
1105	28-29	0.24085155405744985
1105	30-31	0.29730928936267986
1105	32-33	0.2625820949598747
1105	34-35	0.2361030065996914
1105	36-37	0.18610772063092185
1105	38-39	0.2172941276276248
1105	40-41	0.2194046247332082
1105	42-43	0.2794438135607109
1105	44-45	0.3068937308537869
1105	46-47	0.3583688164853669
1105	48-49	0.37688239638683996
1105	50-51	0.320339462099156
1105	52-53	0.2800895231610667
1105	54-55	0.2525723671223403
1105	56-57	0.2287917930201715
1105	58-59	0.25260528809128147
1105	60-61	0.2432637705859122
1105	62-63	0.23707840775595912
1105	64-65	0.2096216249025673
1105	66-67	0.23895126935705946
1105	68-69	0.20880707288991118
1105	70-71	0.22985474983256893
1105	72-73	0.23861920649186175
1105	74-75	0.2039260585569167
1105	76-77	0.149279979240184
1105	78-79	0.15875660648135437
1105	80-81	0.19538949863887467
1105	82-83	0.15385487330338776
1105	84-85	0.20098503114395783
1105	86-87	0.21599230193268681
1105	88-89	0.28852135829826153
1105	90-91	0.17920084503474243
1105	92-93	0.24078522898001609
1105	94-95	0.24684049082559767
1105	96-97	0.2375442338395075
1105	98-99	0.2404554448318308
1105	100	0.22294771950363668
1106	1	0.13887163248398338
1106	2	0.09979421109382969
1106	3	0.1076638779142769
1106	4	0.11113273367997323
1106	5	0.10096999550057006
1106	6	0.11563187279793397
1106	7	0.11765993613575176
1106	8	0.10281752513059672
1106	9	0.13349819189382117
1106	10-11	0.1060581697827061
1106	12-13	0.11489515539533102
1106	14-15	0.23942224214513885
1106	16-17	0.29442813188790495
1106	18-19	0.20586095648947378
1106	20-21	0.19201830179567025
1106	22-23	0.1836091622678282
1106	24-25	0.15584233864066022
1106	26-27	0.17164345407106651
1106	28-29	0.1652895648292514
1106	30-31	0.18920394976380805
1106	32-33	0.18713852795633557
1106	34-35	0.17898643690558202
1106	36-37	0.19226393393473984
1106	38-39	0.16958071434094535
1106	40-41	0.1198926521298489
1106	42-43	0.17922255702211487
1106	44-45	0.19463745132260613
1106	46-47	0.20705805508137587
1106	48-49	0.25450554258137714
1106	50-51	0.14564524523103017
1106	52-53	0.13585785756986013
1106	54-55	0.2043415779898865
1106	56-57	0.1921336506911544
1106	58-59	0.2025578614054595
1106	60-61	0.1629865743563883
1106	62-63	0.10522020300730617
1106	64-65	0.2073323948739656
1106	66-67	0.17270046446330412
1106	68-69	0.13812838341674194
1106	70-71	0.09397201681795764
1106	72-73	0.11952513778538787
1106	74-75	0.14595103185178715
1106	76-77	0.020311776538186166
1106	78-79	0.06842898157577793
1106	80-81	0.13110036516353318
1106	82-83	0.07164231981661828
1106	84-85	0.08139356019594857
1106	86-87	0.1311330163995592
1106	88-89	0.16560419724947906
1106	90-91	0.12380478461143696
1106	92-93	0.04914636670370953
1106	94-95	0.10813419562150273
1106	96-97	0.10161475409154974
1106	98-99	0.14850558078833132
1106	100	0.1520072228255387
1107	1	0.0948731256778288
1107	2	0.08280513976235682
1107	3	0.1032627321165549
1107	4	0.06306350155524143
1107	5	0.06789258857013891
1107	6	0.09414619460704188
1107	7	0.06123491314824747
1107	8	0.059650228460668586
1107	9	0.09933280378266574
1107	10-11	0.0827412589318044
1107	12-13	0.0742391769227666
1107	14-15	0.15712318900493472
1107	16-17	0.24977269266103264
1107	18-19	0.1873305136190453
1107	20-21	0.13703826183584056
1107	22-23	0.13845625398464279
1107	24-25	0.14115708522168546
1107	26-27	0.13241913481906664
1107	28-29	0.08578123235712098
1107	30-31	0.15179501372072934
1107	32-33	0.08860432891505354
1107	34-35	0.11567886017237328
1107	36-37	0.0918757051912209
1107	38-39	0.09474745652850913
1107	40-41	0.08636496842196095
1107	42-43	0.10062803566560774
1107	44-45	0.11309296951518633
1107	46-47	0.13119946862054377
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2216	48-49	-0.5893656263176084
2216	50-51	-0.5793122016932273
2216	52-53	-0.6142901154721798
2216	54-55	-0.5308035190511688
2216	56-57	-0.5828259364130233
2216	58-59	-0.6683204383726249
2216	60-61	-0.6962513512352118
2216	62-63	-0.6759320913371454
2216	64-65	-0.6142064474590541
2216	66-67	-0.6571414385131078
2216	68-69	-0.6526080732527078
2216	70-71	-0.5676027204123244
2216	72-73	-0.5153742966631256
2216	74-75	-0.5507879924609469
2216	76-77	-0.49250707806042016
2216	78-79	-0.48275337120202977
2216	80-81	-0.4945081116095764
2216	82-83	-0.5317474746602571
2216	84-85	-0.4867550438770607
2216	86-87	-0.44533215507107826
2216	88-89	-0.36584089375039497
2216	90-91	-0.46577163011906464
2216	92-93	-0.49487864462707876
2216	94-95	-0.42750843956843454
2216	96-97	-0.4721399188732036
2216	98-99	-0.4573725056875375
2216	100	-0.6484955548273739
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	26.0
8	194.0
9	366.0
10	461.0
11	261.0
12	305.0
13	393.0
14	702.0
15	1250.0
16	2437.0
17	4183.0
18	7077.0
19	10823.0
20	15925.0
21	23097.0
22	33542.0
23	38919.0
24	37379.0
25	32058.0
26	30106.0
27	33392.0
28	40850.0
29	50895.0
30	61805.0
31	78905.0
32	100075.0
33	131638.0
34	190908.0
35	299264.0
36	544937.0
37	1232545.0
38	1587052.0
39	544893.0
40	1313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3981526950018	19.881417624409213	13.420638113550323	24.29979156703867
2	15.383109200043366	20.922921932791738	39.738907246324345	23.95506162084055
3	17.779740076131294	26.449591141708606	30.823092250902402	24.947576531257702
4	12.334406732563526	17.887061940075153	39.009198701016224	30.769332626345097
5	12.713461221434455	38.246555921833085	35.4146469446427	13.625335912089758
6	29.422350770380636	38.73461379756001	18.11556488091994	13.727470551139415
7	25.60667858316193	33.500253796436574	23.26571786244233	17.627349757959166
8	25.457433049855343	34.83990256842933	20.90977653604102	18.792887845674308
9	26.393919783572322	14.619806441253608	21.115303207975906	37.870970567198164
10-11	24.44111217421971	26.11917698266943	28.803874369109057	20.635836474001803
12-13	25.407390517918977	24.169938140032002	28.547236163348778	21.875435178700247
14-15	22.7659685448122	24.985432006689017	26.81125797395706	25.437341474541725
16-17	21.573300853529084	27.837175518230765	27.679350294922383	22.91017333331776
18-19	21.39721316033247	26.792203263915198	30.21990881419035	21.59067476156198
20-21	22.621156685687495	25.95997889825825	29.78326339156294	21.635601024491315
22-23	22.585060732085942	25.855336809669797	29.48578389622684	22.073818562017415
24-25	22.185613556778	26.002583507591314	29.94186815975785	21.869934775872835
26-27	22.094583008869467	26.175100910613796	29.570405625731443	22.15991045478529
28-29	21.841345876405992	26.35129342286665	29.75019051764649	22.057170183080864
30-31	22.27372771332455	26.047820308571673	29.607820296893895	22.07063168120988
32-33	22.276656889446567	26.30214286263419	29.157252674138306	22.263947573780936
34-35	22.46644539895044	26.280793052663416	29.529751931161908	21.723009617224236
36-37	22.396415396418902	26.05902637888008	29.42134389936578	22.12321432533524
38-39	22.408920461418344	26.07642108739873	29.437826304138788	22.076832147044133
40-41	22.382745713681526	25.838941686138618	29.551144606118417	22.227167994061443
42-43	22.36766968160225	25.880025519776662	29.22948647482978	22.522818323791313
44-45	22.277899437878755	26.398717309058274	28.945677443049494	22.37770581001347
46-47	22.62302315152893	26.200278086156885	28.493116744803793	22.6835820175104
48-49	22.872426818166115	26.246432809829166	28.243572732240196	22.63756763976452
50-51	22.594110988451483	26.552420641902568	27.8551904485346	22.99827792111135
52-53	22.380564280101307	26.75572272082562	27.896719760021604	22.966993239051472
54-55	22.417051806103817	26.697490796941825	27.824561314730385	23.060896082223977
56-57	22.74259537182883	26.577797569428697	27.741467494256973	22.9381395644855
58-59	22.332382011819714	26.51485551457826	28.145927341898958	23.006835131703063
60-61	22.38290563356981	26.76425422590722	27.93714419174963	22.915695948773344
62-63	22.438284703380333	26.416257709637573	27.877915172115202	23.267542414866888
64-65	22.10860392449265	26.479119596704027	27.95748743641328	23.454789042390047
66-67	22.396643079967706	26.332473971977787	27.93617616765059	23.334706780403913
68-69	22.13599527040671	26.3248286617246	27.98009872782699	23.5590773400417
70-71	22.259888402182675	26.133103612238994	27.870035264925846	23.736972720652485
72-73	22.500625019524257	26.19811303953734	27.915225845416153	23.386036095522254
74-75	22.309258060460216	26.50209484185822	27.693155396665304	23.49549170101626
76-77	22.28782653394291	26.400461365774262	27.57731000630816	23.734402093974666
78-79	22.10400000155705	26.597771122689075	27.529129961659603	23.769098914094275
80-81	22.0620745796096	26.593177314026605	27.591049512620103	23.75369859374369
82-83	22.31541336602084	26.250010583021837	27.90658680466992	23.527989246287405
84-85	22.523565545951975	26.40915702575528	27.28717146593203	23.780105962360718
86-87	21.851065477923605	26.59002299738263	27.58253444547036	23.97637707922341
88-89	22.20746068101525	26.270091569131505	27.404088691733865	24.118359058119385
90-91	22.028269892321394	26.310131909945067	27.46038079204356	24.201217405689984
92-93	22.184534427824513	26.30061248856188	27.366363411566834	24.14848967204677
94-95	21.627278233513145	26.31089659499896	27.621111987375564	24.44071318411233
96-97	21.77960072297155	26.2734452175245	27.417069979991094	24.529884079512858
98-99	21.69405766458611	26.49628360141741	27.224100016903556	24.585558717092923
100	21.960448151184508	26.23074359509247	26.95130238752055	24.857505866202473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	172.0
2	403.5
3	729.5
4	517.0
5	401.0
6	875.0
7	2237.5
8	4165.5
9	5095.5
10	3881.5
11	2504.5
12	2539.0
13	2889.5
14	3337.5
15	3946.5
16	4591.5
17	5288.5
18	6143.5
19	7012.5
20	8003.5
21	9270.5
22	10956.5
23	13349.0
24	16478.0
25	20819.0
26	26250.5
27	32619.5
28	39180.0
29	46919.0
30	56192.5
31	65484.0
32	77410.5
33	88537.0
34	97840.5
35	107514.5
36	119572.0
37	135112.0
38	144630.5
39	146998.0
40	147435.5
41	149258.5
42	153190.0
43	161323.5
44	171854.0
45	186454.5
46	198843.5
47	205827.5
48	212755.0
49	211170.5
50	203606.0
51	201547.0
52	199027.0
53	193941.5
54	188032.5
55	171555.0
56	154976.0
57	136356.0
58	117510.0
59	104968.5
60	86491.0
61	66681.5
62	51753.5
63	39427.5
64	28243.0
65	19651.0
66	13985.0
67	10038.5
68	7660.5
69	5912.5
70	4728.0
71	3662.0
72	2724.0
73	2281.0
74	1783.0
75	1228.0
76	858.0
77	547.5
78	289.5
79	169.0
80	103.0
81	51.5
82	31.5
83	19.5
84	11.5
85	7.5
86	7.5
87	6.5
88	3.5
89	1.5
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002199309611411186
2	0.003600639629301499
3	1.9462916915143237E-4
4	0.050155936890324125
5	0.0019268287745991808
6	0.004982506730276669
7	0.0
8	2.1409208606657564E-4
9	1.946291691514324E-5
10-11	7.785166766057296E-5
12-13	9.73145845757162E-6
14-15	0.0
16-17	2.7248083681200534E-4
18-19	1.0704604303328782E-4
20-21	2.2382354452414724E-4
22-23	0.0
24-25	0.0
26-27	2.530179198968621E-4
28-29	9.73145845757162E-6
30-31	2.4328646143929048E-4
32-33	2.4328646143929048E-4
34-35	1.3624041840600267E-4
36-37	3.892583383028648E-5
38-39	6.90933550487585E-4
40-41	5.741560489967256E-4
42-43	0.0
44-45	5.352302151664391E-4
46-47	0.0
48-49	6.812020920300134E-5
50-51	0.0
52-53	2.627493783544338E-4
54-55	1.9462916915143237E-4
56-57	1.3624041840600267E-4
58-59	6.90933550487585E-4
60-61	5.060358397937242E-4
62-63	1.459718768635743E-4
64-65	2.4328646143929048E-4
66-67	0.0010120716795874484
68-69	0.0022090410698687576
70-71	0.002948631912644201
72-73	0.002656688158917052
74-75	0.002296624195986902
76-77	0.0036395654631317855
78-79	0.0010315345965025916
80-81	8.46636885808731E-4
82-83	5.741560489967256E-4
84-85	3.795268798452932E-4
86-87	0.0
88-89	0.0
90-91	1.946291691514324E-5
92-93	4.865729228785809E-5
94-95	7.785166766057296E-5
96-97	2.8221229526957696E-4
98-99	8.758312611814458E-5
100	0.07600269055363436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5137976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.56468139613479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.68436990001453	28.826383359038783
2	15.967590692340753	14.231811837361056
3	7.070193748828163	9.452427954764126
4	3.8927766212707597	6.939213994930138
5	2.3178011568177674	5.164603504658821
6	1.511571594083115	4.041762389865696
7	0.9963215473170565	3.108052662700205
8	0.7098565441229006	2.5307624580638683
9	0.5217137134938343	2.0925004879662366
>10	2.2269461332269573	16.38145480103214
>50	0.06988489711517648	2.122614345095687
>100	0.02814097501273739	2.1797219213964456
>500	0.0014591544865508708	0.46991393101657997
>1k	0.0011158240191235928	0.829511885063182
>5k	8.583261685566097E-5	0.34617184173299803
>10k+	1.7166523371132193E-4	1.2830926253139985
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17526	0.34110708185480043	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16018	0.3117570031467644	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9050	0.17613939808204632	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.785166766057296E-5
2	0.0	0.0	0.0	0.0	9.73145845757162E-5
3	0.0	0.0	0.0	0.0	9.73145845757162E-5
4	0.0	0.0	0.0	0.0	1.1677750149085943E-4
5	0.0	0.0	0.0	0.0	1.1677750149085943E-4
6	0.0	0.0	0.0	0.0	2.1409208606657564E-4
7	0.0	0.0	0.0	0.0	2.1409208606657564E-4
8	0.0	0.0	0.0	0.0	2.1409208606657564E-4
9	0.0	0.0	0.0	0.0	3.3086958755743507E-4
10-11	0.0	0.0	0.0	0.0	3.503325044725783E-4
12-13	0.0	0.0	0.0	0.0	3.6979542138772156E-4
14-15	0.0	0.0	0.0	0.0	4.3791563059072285E-4
16-17	0.0	0.0	0.0	0.0	4.963043813361526E-4
18-19	0.0	0.0	0.0	1.946291691514324E-5	5.060358397937242E-4
20-21	0.0	0.0	0.0	1.0704604303328781E-4	5.157672982512958E-4
22-23	0.0	0.0	0.0	2.919437537271486E-4	5.546931320815823E-4
24-25	0.0	0.0	0.0	5.546931320815823E-4	5.838875074542972E-4
26-27	0.0	0.0	0.0	0.0011677750149085943	5.838875074542972E-4
28-29	0.0	0.0	0.0	0.004340230472076942	5.838875074542972E-4
30-31	0.0	0.0	0.0	0.01502537185849058	5.936189659118688E-4
32-33	0.0	0.0	0.0	0.04319794409316042	7.395908427754431E-4
34-35	0.0	0.0	0.0	0.0876804407027203	8.271739688935877E-4
36-37	0.0	0.0	0.0	0.15767882138803296	8.758312611814458E-4
38-39	0.0	0.0	0.0	0.25739707620276936	9.147570950117322E-4
40-41	0.0	0.0	0.0	0.3659806896723535	9.828773042147335E-4
42-43	0.0	0.0	0.0	0.4814931015637286	0.0010315345965025916
44-45	0.0	0.0	0.0	0.6260539169509549	0.0010607289718753065
46-47	0.0	0.0	0.0	0.8007530591812807	0.0010899233472480214
48-49	0.0	0.0	0.0	0.9904581103531819	0.001177506473366166
50-51	0.0	0.0	0.0	1.1951106817159132	0.001226163765654024
52-53	0.0	0.0	0.0	1.398517626396075	0.001304015433314597
54-55	0.0	0.0	0.0	1.6275183068196504	0.0014013300178903133
56-57	0.0	0.0	0.0	1.9033662282579755	0.0014110614763478849
58-59	0.0	0.0	0.0	2.2103061594682423	0.0014305243932630281
60-61	0.0	0.0	0.0	2.533653329637974	0.001459718768635743
62-63	0.0	0.0	0.0	2.864260167817055	0.001459718768635743
64-65	0.0	0.0	0.0	3.213132953520997	0.0014791816855508862
66-67	0.0	0.0	0.0	3.610205652965292	0.0014791816855508862
68-69	0.0	0.0	0.0	4.050447102127374	0.001508376060923601
70-71	0.0	0.0	0.0	4.523288937122322	0.0015959591870417457
72-73	0.0	0.0	0.0	5.007857179558643	0.0017321996054477484
74-75	0.0	0.0	0.0	5.492756291582522	0.0017321996054477484
76-77	0.0	0.0	0.0	6.032209959719547	0.0017516625223628916
78-79	0.0	0.0	0.0	6.616632697389011	0.0018100512731083214
80-81	0.0	0.0	0.0	7.2452070620804765	0.0018587085653961792
82-83	0.0	0.0	0.0	7.889449074888633	0.00204360627609004
84-85	0.0	0.0	0.0	8.534352826871904	0.0020825321099203265
86-87	0.0	0.0	0.0	9.216129074950913	0.002121457943750613
88	0.0	0.0	0.0	9.750395875730055	0.0021409208606657563
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9655	0.0	62.688263	1
GTATCAA	18500	0.0	43.698257	1
ATCAACG	22215	0.0	35.753796	3
CAACGCA	22325	0.0	35.558933	5
TATCAAC	23485	0.0	34.26153	2
TCAACGC	24035	0.0	33.261314	4
AACGCAG	24350	0.0	33.219772	6
ACGCAGA	26250	0.0	30.275774	7
CGCAGAG	26795	0.0	29.642435	8
GCAGAGT	29965	0.0	26.208557	9
TACATGG	16830	0.0	25.734917	2
GTACATG	17420	0.0	25.67393	1
GAGTACT	20200	0.0	24.255362	12-13
GTGGTAT	3195	0.0	24.139763	1
ACATGGG	17780	0.0	23.751085	3
CAGAGTA	28775	0.0	23.707676	10-11
CTAACGC	980	0.0	23.033188	3
GTACTAG	1975	0.0	22.859364	1
GTACTTT	21780	0.0	22.31248	14-15
CATGGGG	13705	0.0	22.200352	4
>>END_MODULE