FastQCFastQC Report
Mon 16 Jan 2017
ERR1146579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4984547
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.039.8950961
GTATCAA116100.032.7246931
CAACGCA112000.031.1846375
ATCAACG115400.030.349153
AACGCAG133600.027.8948026
TACATGG237300.027.4380022
TATCAAC137600.027.4003662
GTACATG242750.027.3315451
ACGCAGA134550.025.7768127
CGCAGAG133850.025.77048
ACATGGG248350.025.55443
TATTCTC29450.024.5813265
CATGGGG182550.024.1550754
TCAACGC147350.023.991374
ATGGGGG135550.022.6802655
GCAGAGT146750.022.6077299
GTGGTAT18950.022.5802441
GGGCACC40700.021.97181394
GTACTAG43250.021.8527621
CTGTGCG46100.021.6105239
Produced by FastQC (version 0.11.5)