##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146579_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4984547
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14898164266482	31.0	31.0	34.0	27.0	34.0
2	31.326049287929273	33.0	31.0	34.0	27.0	34.0
3	31.292275105440876	33.0	31.0	34.0	27.0	34.0
4	34.74090163057947	37.0	35.0	37.0	31.0	37.0
5	34.829505068364284	37.0	35.0	37.0	32.0	37.0
6	34.7836591770526	37.0	35.0	37.0	31.0	37.0
7	34.726184144717664	37.0	35.0	37.0	31.0	37.0
8	34.72833840266728	37.0	35.0	37.0	31.0	37.0
9	36.21274490941705	39.0	37.0	39.0	31.0	39.0
10-11	36.23023215549979	39.0	37.0	39.0	31.0	39.0
12-13	36.17141196582156	39.0	37.0	39.0	31.0	39.0
14-15	37.47809490009824	40.0	38.0	41.0	31.0	41.0
16-17	37.524558600811666	40.0	38.0	41.0	31.0	41.0
18-19	37.56327616130413	40.0	38.0	41.0	31.0	41.0
20-21	37.689282195553574	40.0	38.0	41.0	31.0	41.0
22-23	37.72695372317685	40.0	38.0	41.0	31.0	41.0
24-25	37.76616380585838	40.0	38.0	41.0	31.0	41.0
26-27	37.854823417253364	40.0	38.0	41.0	31.0	41.0
28-29	37.86630339727964	40.0	38.0	41.0	31.0	41.0
30-31	37.84259061054094	40.0	38.0	41.0	31.0	41.0
32-33	37.81040654246013	40.0	38.0	41.0	31.0	41.0
34-35	37.756879110579156	40.0	38.0	41.0	30.5	41.0
36-37	37.673507642720594	40.0	37.0	41.0	30.0	41.0
38-39	37.59736812593	40.0	37.0	41.0	30.0	41.0
40-41	37.52463975161635	40.0	37.0	41.0	30.0	41.0
42-43	37.348339076750605	40.0	37.0	41.0	30.0	41.0
44-45	37.185230874540856	40.0	36.0	41.0	30.0	41.0
46-47	37.05540994999144	40.0	36.0	41.0	30.0	41.0
48-49	36.91543464230551	40.0	35.5	41.0	30.0	41.0
50-51	36.74511344762122	39.0	35.0	41.0	30.0	41.0
52-53	36.58389167561265	39.0	35.0	41.0	29.5	41.0
54-55	36.454765698868925	39.0	35.0	41.0	29.0	41.0
56-57	36.23493488977033	39.0	35.0	41.0	29.0	41.0
58-59	36.014807463948074	38.0	35.0	40.0	29.0	41.0
60-61	35.79181859454831	37.5	35.0	40.0	29.0	41.0
62-63	35.54611211409984	37.0	34.5	40.0	28.5	41.0
64-65	35.24775772000946	36.5	34.0	40.0	28.0	41.0
66-67	35.02110041293622	36.0	34.0	39.5	28.0	41.0
68-69	34.777970896853816	36.0	34.0	39.0	28.0	41.0
70-71	34.55098667943145	35.0	34.0	39.0	28.0	41.0
72-73	34.24379988793365	35.0	34.0	38.5	27.5	40.5
74-75	33.931707535308625	35.0	33.5	37.5	27.0	40.0
76-77	33.68438205116734	35.0	33.0	37.0	27.0	39.0
78-79	33.39416831659928	35.0	33.0	36.5	26.0	39.0
80-81	33.18258299099196	35.0	33.0	36.0	26.0	39.0
82-83	32.904873201115365	35.0	33.0	36.0	26.0	37.0
84-85	32.70200361236437	35.0	33.0	35.5	26.0	37.0
86-87	32.49285762577823	35.0	33.0	35.0	25.0	37.0
88-89	32.32540770505324	35.0	33.0	35.0	25.0	36.0
90-91	32.165471807167236	35.0	33.0	35.0	25.0	36.0
92-93	31.9895184055843	35.0	32.5	35.0	25.0	36.0
94-95	31.861031203036106	35.0	32.0	35.0	24.0	35.5
96-97	31.745721326331157	35.0	32.0	35.0	24.0	35.0
98-99	31.628095090687278	35.0	32.0	35.0	24.0	35.0
100	30.39224948626224	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36134925338258483
1101	2	0.35177318730848484
1101	3	0.34441784513602514
1101	4	0.3146343309410824
1101	5	0.2256466420710055
1101	6	0.2849180653125387
1101	7	0.3215135249687293
1101	8	0.33153514680072504
1101	9	0.4380232261809667
1101	10-11	0.420259349858469
1101	12-13	0.4010828790546128
1101	14-15	0.419273399612095
1101	16-17	0.41459616262331167
1101	18-19	0.510000866237938
1101	20-21	0.4228227054027869
1101	22-23	0.44244413821264317
1101	24-25	0.41469458745891075
1101	26-27	0.4174039858291039
1101	28-29	0.3800678157184407
1101	30-31	0.49379611866934425
1101	32-33	0.4384060348612948
1101	34-35	0.39909551146728717
1101	36-37	0.45763788670539896
1101	38-39	0.41031907631938935
1101	40-41	0.40556623492037147
1101	42-43	0.4642787285053558
1101	44-45	0.3983492934316786
1101	46-47	0.2974276909769884
1101	48-49	0.35655496027531086
1101	50-51	0.3176963029651887
1101	52-53	0.28630303649845956
1101	54-55	0.28021299628792207
1101	56-57	0.2902726520824572
1101	58-59	0.2462900173782998
1101	60-61	0.23344009246162756
1101	62-63	0.22376744189429587
1101	64-65	0.25437317424164263
1101	66-67	0.23693445535596425
1101	68-69	0.18935195954971817
1101	70-71	0.22155788101312623
1101	72-73	0.23092520990986998
1101	74-75	0.28822145164706825
1101	76-77	0.27127179596126183
1101	78-79	0.26593565498193783
1101	80-81	0.28643418045994196
1101	82-83	0.2521571848536581
1101	84-85	0.14644359276868357
1101	86-87	0.23823506437308595
1101	88-89	0.21577214714790927
1101	90-91	0.2534727065788971
1101	92-93	0.22688384534286143
1101	94-95	0.2299244587055469
1101	96-97	0.22405336522175645
1101	98-99	0.185344760128082
1101	100	0.25086844126285257
1102	1	0.3421715372543126
1102	2	0.41025222536232064
1102	3	0.34513913672819996
1102	4	0.3450327855917479
1102	5	0.39664473396593536
1102	6	0.4243324166379665
1102	7	0.4045267881415384
1102	8	0.38403715505600644
1102	9	0.4921457298485308
1102	10-11	0.5083076197801972
1102	12-13	0.5156341994048859
1102	14-15	0.5968989726116689
1102	16-17	0.5546667174478657
1102	18-19	0.5347517652508174
1102	20-21	0.5570347828886923
1102	22-23	0.5387875955966734
1102	24-25	0.5445763376455091
1102	26-27	0.5140133329661793
1102	28-29	0.5360479579069235
1102	30-31	0.5680138490573228
1102	32-33	0.5841567967762558
1102	34-35	0.541826443639529
1102	36-37	0.5435044524389809
1102	38-39	0.5428206520861281
1102	40-41	0.5656820606975614
1102	42-43	0.5971189676209221
1102	44-45	0.4812944134047612
1102	46-47	0.5012804000832887
1102	48-49	0.4908021714880064
1102	50-51	0.5185964424117131
1102	52-53	0.48859367910769436
1102	54-55	0.47646923037630984
1102	56-57	0.5335266662539482
1102	58-59	0.5209381884291417
1102	60-61	0.521785858169693
1102	62-63	0.45258340379559314
1102	64-65	0.5360484964074175
1102	66-67	0.4948462030621954
1102	68-69	0.4258505122883989
1102	70-71	0.4172380842846479
1102	72-73	0.4608696995762571
1102	74-75	0.5127138647669867
1102	76-77	0.4451390387828553
1102	78-79	0.38989885202123276
1102	80-81	0.36951966826404714
1102	82-83	0.3815776377999569
1102	84-85	0.38938094990664496
1102	86-87	0.3926949091011096
1102	88-89	0.39934501039198267
1102	90-91	0.4011087557302133
1102	92-93	0.43100900790795293
1102	94-95	0.45700461878549703
1102	96-97	0.5131811188991939
1102	98-99	0.45456892342506094
1102	100	0.3617078547358368
1103	1	0.0671740684644071
1103	2	0.15167958834220485
1103	3	0.10663890501814421
1103	4	0.13043162265040564
1103	5	0.08847425996010116
1103	6	0.19872457239067387
1103	7	0.19163750345243358
1103	8	0.19132079828565907
1103	9	0.24892764913359144
1103	10-11	0.21358658090625227
1103	12-13	0.24834644273201434
1103	14-15	0.29399904050978876
1103	16-17	0.27791141850924106
1103	18-19	0.28559010384798
1103	20-21	0.2597604878987525
1103	22-23	0.20785405809719748
1103	24-25	0.28060410329645435
1103	26-27	0.23067770430612455
1103	28-29	0.23663134286242382
1103	30-31	0.25179884462452407
1103	32-33	0.2967060465038145
1103	34-35	0.27204172532601234
1103	36-37	0.2707498716745107
1103	38-39	0.3058415959897971
1103	40-41	0.21253456899067658
1103	42-43	0.23776184599328332
1103	44-45	0.18451157236003723
1103	46-47	0.2230446154136061
1103	48-49	0.15858855840425434
1103	50-51	0.16005810827818578
1103	52-53	0.15025217876602426
1103	54-55	0.0736160757627431
1103	56-57	0.16075644460703842
1103	58-59	0.09793329003862539
1103	60-61	0.1510269052160922
1103	62-63	0.24328335687295066
1103	64-65	0.10016177994395292
1103	66-67	0.1422726791205804
1103	68-69	0.1810213934652296
1103	70-71	0.1676024787411734
1103	72-73	0.1676638938694026
1103	74-75	0.20722257231116004
1103	76-77	0.219279706565068
1103	78-79	0.23203226838551672
1103	80-81	0.1821431668209854
1103	82-83	0.14340437674128736
1103	84-85	0.15519584169604883
1103	86-87	0.1915613261668625
1103	88-89	0.14482203734691268
1103	90-91	0.12725913174646308
1103	92-93	0.1208874608323427
1103	94-95	0.1814974604507782
1103	96-97	0.2040024249019936
1103	98-99	0.1408043843901723
1103	100	0.15046648761384063
1104	1	0.22748611066435842
1104	2	0.21745852259791576
1104	3	0.1264630960107489
1104	4	0.16606976456589706
1104	5	0.18351102875340075
1104	6	0.21134233672600544
1104	7	0.3193940425543218
1104	8	0.29101117269252086
1104	9	0.25612761197093903
1104	10-11	0.3482254938261917
1104	12-13	0.3336486340703999
1104	14-15	0.4186994855481032
1104	16-17	0.35812070148545416
1104	18-19	0.3130595071408706
1104	20-21	0.36414225971969216
1104	22-23	0.31751282787196544
1104	24-25	0.406224026720011
1104	26-27	0.3824421849433648
1104	28-29	0.4335677981690793
1104	30-31	0.40717901376287813
1104	32-33	0.4236462266944301
1104	34-35	0.3977026724600705
1104	36-37	0.43154758375803937
1104	38-39	0.40147728079187317
1104	40-41	0.42737358626251876
1104	42-43	0.4299015436209501
1104	44-45	0.37466420555652746
1104	46-47	0.34365303151399473
1104	48-49	0.3616796691350075
1104	50-51	0.41979988211929964
1104	52-53	0.319519628020295
1104	54-55	0.36653101378849584
1104	56-57	0.32027453580482046
1104	58-59	0.3279545060193314
1104	60-61	0.36119721308180885
1104	62-63	0.41080275221102625
1104	64-65	0.44169061211440663
1104	66-67	0.329334098165198
1104	68-69	0.34716005222777113
1104	70-71	0.3329633029592287
1104	72-73	0.3674110981811012
1104	74-75	0.3382799903374192
1104	76-77	0.305535182066933
1104	78-79	0.3353686681550272
1104	80-81	0.3590939864592073
1104	82-83	0.32237003369033346
1104	84-85	0.2663528602927201
1104	86-87	0.2649579153038957
1104	88-89	0.31043053359867656
1104	90-91	0.2590429969487573
1104	92-93	0.2605647921124259
1104	94-95	0.29684816232681754
1104	96-97	0.22912397488120106
1104	98-99	0.3050035524362187
1104	100	0.2971739556355999
1105	1	0.09265720851161774
1105	2	0.13314601931795167
1105	3	0.08918232510008295
1105	4	0.09520139882680922
1105	5	0.05098106717842654
1105	6	0.14013063327950448
1105	7	0.052680322893778
1105	8	0.1336408638456632
1105	9	0.17627804271685932
1105	10-11	0.2034695682410046
1105	12-13	0.16030020030399328
1105	14-15	0.2406746597189482
1105	16-17	0.18502828844552965
1105	18-19	0.23116048289747226
1105	20-21	0.22556379484603184
1105	22-23	0.2079138525022728
1105	24-25	0.26348945205555907
1105	26-27	0.22742895857765433
1105	28-29	0.21738710777052006
1105	30-31	0.24792029794149784
1105	32-33	0.246585636186893
1105	34-35	0.2008227635450197
1105	36-37	0.2269844746935945
1105	38-39	0.22254485419852443
1105	40-41	0.1987018373013143
1105	42-43	0.20166762907485491
1105	44-45	0.15102629790026612
1105	46-47	0.1259432675655603
1105	48-49	0.18066533003063512
1105	50-51	0.133775692310806
1105	52-53	0.11564529672160262
1105	54-55	0.03931509524738175
1105	56-57	0.13191588163785894
1105	58-59	0.11553670645326264
1105	60-61	0.17413661186417784
1105	62-63	0.13631273395508714
1105	64-65	0.09542742387858993
1105	66-67	0.09877906918905666
1105	68-69	0.09603896367647025
1105	70-71	0.061125261569650036
1105	72-73	0.09852325015425833
1105	74-75	0.12375080568028096
1105	76-77	0.08405269978707253
1105	78-79	0.08752502570589371
1105	80-81	0.0025168232184427097
1105	82-83	0.04431752227942809
1105	84-85	0.09670154947492904
1105	86-87	0.030373294429367093
1105	88-89	0.02804114988381201
1105	90-91	0.04033314862861204
1105	92-93	0.08802838147679637
1105	94-95	0.05679323424368121
1105	96-97	0.08319853725602755
1105	98-99	-0.0018234890962780526
1105	100	0.18955685260616306
1106	1	0.12234447146280303
1106	2	0.10300955547819868
1106	3	0.10389193085143589
1106	4	0.07650396274682691
1106	5	0.032148644818846606
1106	6	0.10088307584464928
1106	7	0.16933847169453742
1106	8	0.20097878919865053
1106	9	0.17630267813665768
1106	10-11	0.19723013964713232
1106	12-13	0.2284267981101351
1106	14-15	0.2822806348253124
1106	16-17	0.28511013341245217
1106	18-19	0.2688560088848746
1106	20-21	0.25660219191644984
1106	22-23	0.2801919140676574
1106	24-25	0.2956869569282574
1106	26-27	0.2816929344462409
1106	28-29	0.30524452971339144
1106	30-31	0.2466017096979627
1106	32-33	0.28512780696854634
1106	34-35	0.26543629963463644
1106	36-37	0.2863687756593123
1106	38-39	0.24474809132659203
1106	40-41	0.2746000907574029
1106	42-43	0.27830994566316747
1106	44-45	0.2123725331871711
1106	46-47	0.29570831854986324
1106	48-49	0.2530329210058184
1106	50-51	0.27734114384669084
1106	52-53	0.2420874929014758
1106	54-55	0.21856831430852708
1106	56-57	0.2533501659492501
1106	58-59	0.18120846378081268
1106	60-61	0.258003999851411
1106	62-63	0.22771570698692756
1106	64-65	0.2892291579070232
1106	66-67	0.2661253852756147
1106	68-69	0.23216635478834036
1106	70-71	0.22523255673334575
1106	72-73	0.291081799534858
1106	74-75	0.29012724337032836
1106	76-77	0.2510559281006337
1106	78-79	0.27122115232290867
1106	80-81	0.2752454700021829
1106	82-83	0.23518091746404934
1106	84-85	0.2486607292623617
1106	86-87	0.20920697681156497
1106	88-89	0.19561822526961237
1106	90-91	0.2544499222135741
1106	92-93	0.25307976130044096
1106	94-95	0.21152646965686728
1106	96-97	0.2447625078415605
1106	98-99	0.21779037329964623
1106	100	0.27019649207018404
1107	1	0.04143301709908087
1107	2	0.07059940523349084
1107	3	0.09083546072804083
1107	4	0.03607464078782385
1107	5	0.10771274347440851
1107	6	0.05337372395666762
1107	7	0.12595066417549816
1107	8	0.12053123763875817
1107	9	0.1933134748369909
1107	10-11	0.19968766887768652
1107	12-13	0.1603913155072263
1107	14-15	0.20253450467168932
1107	16-17	0.2102751362474251
1107	18-19	0.21034760222866566
1107	20-21	0.21749587342819154
1107	22-23	0.26179853476227777
1107	24-25	0.2986388939196587
1107	26-27	0.26884556910783175
1107	28-29	0.2789708560415818
1107	30-31	0.22873560022826922
1107	32-33	0.26047011642675955
1107	34-35	0.28464720445055036
1107	36-37	0.21953025033827345
1107	38-39	0.22772855402974557
1107	40-41	0.23063680713265455
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2216	18-19	-0.22165091146753468
2216	20-21	-0.2340698838371722
2216	22-23	-0.2922738967837404
2216	24-25	-0.2487143189824934
2216	26-27	-0.37988901443150525
2216	28-29	-0.3757399329153941
2216	30-31	-0.35614024406512357
2216	32-33	-0.32332537681166684
2216	34-35	-0.3019361010383079
2216	36-37	-0.22250813460331642
2216	38-39	-0.22581298591354226
2216	40-41	-0.26734850091044393
2216	42-43	-0.16681719978911502
2216	44-45	-0.06847076063429824
2216	46-47	-0.14774669723396983
2216	48-49	-0.11396471664476593
2216	50-51	-0.12305177416646984
2216	52-53	-0.10745593658729291
2216	54-55	-0.07189844500360465
2216	56-57	-0.03369333230842386
2216	58-59	-0.03216175169141877
2216	60-61	-0.07900302337927201
2216	62-63	-0.05014004555944496
2216	64-65	-0.061573897853001824
2216	66-67	-0.05703103042306168
2216	68-69	-0.11440163675253956
2216	70-71	-0.10607655014014483
2216	72-73	-0.05490501223970767
2216	74-75	0.002006218900703516
2216	76-77	-0.05208675582317568
2216	78-79	0.037285623446024374
2216	80-81	-0.04899815457022072
2216	82-83	-0.04193840263704374
2216	84-85	-0.029936187029925065
2216	86-87	-0.09598237974255142
2216	88-89	-0.09012383522282619
2216	90-91	-0.13390205574517466
2216	92-93	-0.09948454862836087
2216	94-95	-0.17119328000230993
2216	96-97	-0.14246011965873961
2216	98-99	-0.06138992336390814
2216	100	-0.19661431422046505
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1489.0
1	349.0
2	300.0
3	256.0
4	207.0
5	294.0
6	489.0
7	1313.0
8	3774.0
9	5190.0
10	5579.0
11	5956.0
12	7105.0
13	9639.0
14	14363.0
15	16631.0
16	17730.0
17	19087.0
18	20857.0
19	22507.0
20	24349.0
21	27255.0
22	30534.0
23	35971.0
24	44794.0
25	55746.0
26	57982.0
27	50936.0
28	52735.0
29	63997.0
30	74492.0
31	84428.0
32	105738.0
33	142621.0
34	223683.0
35	344437.0
36	642025.0
37	1199187.0
38	1184184.0
39	385995.0
40	343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63405050728587	21.507125618368242	12.882583031135999	23.976240843209887
2	15.333742327347247	21.034671878663683	40.410308255148024	23.221277538841047
3	17.800002529849717	28.059426571413137	30.04652313316667	24.094047765570473
4	12.229118143121035	18.278491687414665	40.398160790297965	29.094229379166332
5	12.782753961035178	39.31710167276057	35.1972235106086	12.702920855595645
6	28.086491891761646	39.76295751909593	19.054219577038303	13.096331012104127
7	25.152189566014272	33.640349520972826	24.066391957756778	17.141068955256127
8	25.21668328248191	35.23522673308977	21.099013191250542	18.449076793177777
9	26.406978901239036	15.572251591863088	21.708985182764113	36.31178432413376
10-11	24.565359027668325	27.143539790848703	28.02785003026741	20.263251151215563
12-13	24.868588078961785	25.628310894817663	27.486457289594874	22.016643736625678
14-15	22.76838861872699	26.316967716397972	25.3811270424144	25.53351662246064
16-17	21.797395194077456	28.656940992413908	26.708847975620426	22.836815837888206
18-19	21.401705777392817	29.00985081525269	28.10390629407724	21.48453711327725
20-21	23.452639803847543	27.866431467348026	26.72143586554049	21.959492863263947
22-23	23.623954724583147	27.74526685397911	26.355481328367738	22.275297093070005
24-25	22.944090728073473	27.88848041097957	27.12038332876067	22.04704553218628
26-27	22.672456671580342	28.173533514120415	26.58833898230224	22.565670831997004
28-29	22.01659473859788	28.706612888183436	26.708592386650924	22.568199986567762
30-31	22.781256045611887	28.203977275978676	26.702512073152818	22.31225460525662
32-33	22.992055516908444	28.52232172752022	25.9055848317609	22.58003792381043
34-35	22.986874906503914	28.247815580037166	26.890859822636042	21.874449690822875
36-37	22.842880499905373	27.969731789803205	26.695059428520366	22.492328281771055
38-39	22.815652316394072	27.929769232066707	27.102220290967914	22.1523581605713
40-41	22.679489139788	27.315596464566706	27.52410940257916	22.480804993066133
42-43	22.36576372101653	27.661108685304292	26.994393892950736	22.97873370072844
44-45	22.285472929048872	28.45438464441507	26.973745836371627	22.286396590164433
46-47	22.328052048838202	27.96520707243356	26.760987867753546	22.94575301097469
48-49	22.891072105763648	27.42336955959692	27.074092567087998	22.61146576755143
50-51	22.66097230973864	27.452461015201152	26.67855818995032	23.208008485109893
52-53	21.930618743467914	28.066072854526404	26.760584451554802	23.24272395045088
54-55	22.21160010019733	27.8396280450125	26.658514338609756	23.29025751618041
56-57	22.83381467661242	27.42273104979423	26.540338655218846	23.2031156183745
58-59	22.166779376466295	27.61448328455936	27.26378260112127	22.954954737853072
60-61	22.302297978405953	28.078797355522	26.63740362167333	22.98150104439872
62-63	22.651148901544012	27.413052707171182	26.291562860343326	23.64423553094148
64-65	21.900518987537513	27.519704275707834	26.768404396985	23.81137233976965
66-67	22.69204893703333	27.178358697529454	26.47289063472746	23.656701730709756
68-69	22.063548707501955	27.210627317714618	26.801597001515386	23.924226973268038
70-71	22.175733435968365	26.9706242036656	26.97003176953147	23.88361059083456
72-73	22.61868853407149	27.081176193693135	26.976217711184137	23.323917561051235
74-75	22.525844500015666	27.37648301119252	26.887297174195425	23.210375314596387
76-77	22.206234530401996	27.36498963254527	26.75423860709606	23.67453722995667
78-79	22.20522366926937	27.251132816595174	26.787291567169063	23.75635194696639
80-81	21.98686758902012	27.416297749383666	27.23672921993575	23.360105441660462
82-83	22.627005639584922	26.510939505382265	27.94579467275576	22.916260182277057
84-85	22.90087235795314	27.00103306075578	26.763382896417987	23.334711684873092
86-87	21.81207814458473	27.356679484722008	27.301011966006268	23.530230404686996
88-89	22.774390507172175	26.418796242502644	27.003071135761953	23.803742114563224
90-91	22.627063895279964	26.36888979659214	27.180872523722282	23.82317378440562
92-93	22.887529912791297	26.486221691911854	27.104487987994926	23.521760407301922
94-95	21.90891851619213	26.492029413822976	27.528505321438455	24.07054674854643
96-97	22.139299861155358	26.574657785455557	27.112134845065583	24.1739075083235
98-99	21.857957188794646	27.029597950948936	26.88260884278361	24.229836017472806
100	22.62885948989151	26.502598165778217	26.292778028273933	24.57576431605634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2666.0
1	1748.0
2	898.5
3	960.5
4	885.5
5	780.0
6	696.5
7	651.0
8	696.0
9	737.0
10	659.0
11	601.5
12	585.5
13	587.0
14	652.0
15	719.0
16	786.5
17	864.5
18	1118.5
19	1515.5
20	1963.0
21	2672.5
22	3834.5
23	5402.0
24	7202.0
25	9223.0
26	12557.0
27	17044.0
28	22411.0
29	32447.0
30	46787.0
31	60232.5
32	78530.0
33	97238.5
34	113112.0
35	127814.0
36	140251.5
37	161161.0
38	167093.5
39	156257.5
40	149723.0
41	142217.5
42	141351.0
43	151135.0
44	163825.0
45	187176.5
46	207321.0
47	220315.5
48	230026.0
49	226101.0
50	216803.5
51	214245.0
52	211122.0
53	201037.0
54	187614.5
55	163103.0
56	143228.5
57	118270.0
58	93320.0
59	83951.5
60	67938.0
61	49191.5
62	36832.0
63	29719.0
64	21522.0
65	11675.5
66	7374.5
67	5645.5
68	4547.5
69	3811.0
70	3064.5
71	2417.0
72	1800.5
73	1541.0
74	1204.0
75	880.5
76	713.0
77	524.0
78	366.0
79	241.5
80	174.5
81	127.5
82	89.5
83	68.0
84	67.5
85	59.0
86	47.0
87	41.0
88	36.0
89	25.5
90	23.5
91	24.0
92	25.0
93	23.0
94	20.5
95	18.0
96	16.0
97	14.0
98	11.0
99	9.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0984843758118842
2	0.06457958967986459
3	0.0805288825644537
4	0.08319712904703276
5	0.0833977490833169
6	0.08881449006298868
7	0.08516320540261732
8	0.08730983979085762
9	0.07204265502963458
10-11	0.07908441830320789
12-13	0.06929416053254188
14-15	0.07807128711997299
16-17	0.07992702245560128
18-19	0.07912454231046473
20-21	0.07068846978471664
22-23	0.07508204857933931
24-25	0.07746942701112057
26-27	0.0712100818790554
28-29	0.08009754948644279
30-31	0.0838290821613278
32-33	0.08623652259673747
34-35	0.08811231993599418
36-37	0.08836309498134935
38-39	0.08985771425166621
40-41	0.08587540653142603
42-43	0.09091096944215793
44-45	0.0875806768398412
46-47	0.08475193432823484
48-49	0.09406070401181893
50-51	0.0908608144330869
52-53	0.09632771042182971
54-55	0.08776123487249694
56-57	0.09495346317328335
58-59	0.09919657694069291
60-61	0.0980530427338733
62-63	0.09872511985542518
64-65	0.1011927463017201
66-67	0.1020554124577419
68-69	0.09960784801507538
70-71	0.10212562947044135
72-73	0.09948747599330492
74-75	0.10333938068996039
76-77	0.10075138222189499
78-79	0.10763264946644098
80-81	0.10253690054482384
82-83	0.10770286647914043
84-85	0.10370049675527185
86-87	0.09995893307857265
88-89	0.09942728998241968
90-91	0.09922666994613552
92-93	0.09336856488663865
94-95	0.0931579138485403
96-97	0.09069028740224538
98-99	0.10565654210904221
100	0.24461601024125162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4984547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.40815548685668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11455179257872	36.74574332236731
2	14.350100017995086	15.041245459900624
3	6.004905795128211	9.44118109785018
4	3.0800349552668043	6.456758033623052
5	1.8027643453887914	4.723977705964858
6	1.1573241949117203	3.6391935813380796
7	0.7751448204633745	2.843673719297329
8	0.5552892512920014	2.328134833751315
9	0.4072458452785246	1.9208703222659969
>10	1.7024507661640973	14.420258324762141
>50	0.04150683692076574	1.4255321783191657
>100	0.008383102190857708	0.691878772731545
>500	7.456910529000173E-5	0.019891868956580863
>1k	2.237073158261995E-4	0.30166077887186815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.0062003628413978E-5	0.0
6	0.0	0.0	0.0	2.0062003628413978E-5	0.0
7	0.0	0.0	0.0	2.0062003628413978E-5	0.0
8	0.0	0.0	0.0	2.0062003628413978E-5	0.0
9	0.0	0.0	0.0	2.0062003628413978E-5	0.0
10-11	0.0	0.0	0.0	2.0062003628413978E-5	0.0
12-13	0.0	0.0	0.0	2.0062003628413978E-5	0.0
14-15	0.0	0.0	0.0	2.0062003628413978E-5	0.0
16-17	0.0	0.0	0.0	2.0062003628413978E-5	0.0
18-19	0.0	0.0	0.0	3.0093005442620968E-5	0.0
20-21	0.0	0.0	0.0	6.0186010885241936E-5	0.0
22-23	0.0	0.0	0.0	1.6049602902731182E-4	0.0
24-25	0.0	0.0	0.0	5.717671034097982E-4	0.0
26-27	0.0	0.0	0.0	0.0015447742793878762	0.0
28-29	0.0	0.0	0.0	0.005787888046797432	0.0
30-31	0.0	0.0	0.0	0.02198795597674172	0.0
32-33	0.0	0.0	0.0	0.0621621182426407	0.0
34-35	0.0	0.0	0.0	0.12390293440908473	0.0
36-37	0.0	0.0	0.0	0.22397220850761362	0.0
38-39	0.0	0.0	0.0	0.3688198747047625	0.0
40-41	0.0	0.0	0.0	0.5277510674490581	0.0
42-43	0.0	0.0	0.0	0.6941252635394952	0.0
44-45	0.0	0.0	0.0	0.9008943039357438	0.0
46-47	0.0	0.0	0.0	1.1498236449571044	0.0
48-49	0.0	0.0	0.0	1.4259269698931516	0.0
50-51	0.0	0.0	0.0	1.7144085510679306	0.0
52-53	0.0	0.0	0.0	1.9960690510090484	0.0
54-55	0.0	0.0	0.0	2.3066990841895962	0.0
56-57	0.0	0.0	0.0	2.671988046255758	0.0
58-59	0.0	0.0	0.0	3.0944537186628995	0.0
60-61	0.0	0.0	0.0	3.529568484357756	0.0
62-63	0.0	0.0	0.0	3.9773824983493986	0.0
64-65	0.0	0.0	0.0	4.444446004822504	0.0
66-67	0.0	0.0	0.0	4.966960889324547	0.0
68-69	0.0	0.0	0.0	5.547374716298191	0.0
70-71	0.0	0.0	0.0	6.169718130855221	0.0
72-73	0.0	0.0	0.0	6.810077224670566	0.0
74-75	0.0	0.0	0.0	7.450315946464142	0.0
76-77	0.0	0.0	0.0	8.159317185694107	0.0
78-79	0.0	0.0	0.0	8.921934129621008	0.0
80-81	0.0	0.0	0.0	9.735859647827576	0.0
82-83	0.0	0.0	0.0	10.572104145070757	0.0
84-85	0.0	0.0	0.0	11.400042972811772	0.0
86-87	0.0	0.0	0.0	12.266039421435888	0.0
88	0.0	0.0	0.0	12.938608062076653	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7390	0.0	39.895096	1
GTATCAA	11610	0.0	32.724693	1
CAACGCA	11200	0.0	31.184637	5
ATCAACG	11540	0.0	30.34915	3
AACGCAG	13360	0.0	27.894802	6
TACATGG	23730	0.0	27.438002	2
TATCAAC	13760	0.0	27.400366	2
GTACATG	24275	0.0	27.331545	1
ACGCAGA	13455	0.0	25.776812	7
CGCAGAG	13385	0.0	25.7704	8
ACATGGG	24835	0.0	25.5544	3
TATTCTC	2945	0.0	24.581326	5
CATGGGG	18255	0.0	24.155075	4
TCAACGC	14735	0.0	23.99137	4
ATGGGGG	13555	0.0	22.680265	5
GCAGAGT	14675	0.0	22.607729	9
GTGGTAT	1895	0.0	22.580244	1
GGGCACC	4070	0.0	21.971813	94
GTACTAG	4325	0.0	21.852762	1
CTGTGCG	4610	0.0	21.610523	9
>>END_MODULE