FastQCFastQC Report
Mon 16 Jan 2017
ERR1146579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4984547
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213020.42736080129247456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200380.40200242870615926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106670.21400139270429186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128200.057.661961
GTATCAA241000.043.1611441
GTGGTAT23850.038.6449361
ATCAACG294050.034.492743
CAACGCA295800.034.2096375
TATCAAC313250.033.1751562
AACGCAG321200.032.1911666
TCAACGC331350.030.7943824
ACGCAGA345650.029.2738327
CGCAGAG346400.029.1697528
GCAGAGT370400.027.0513139
TACATGG223000.025.4093762
GTACATG228050.025.3217181
ACATGGG231550.023.6987153
GCTATTG53750.023.6079048
GAGTACT255700.023.53560312-13
GGGCACC36800.023.51700894
CAGAGTA368800.023.1420310-11
CTGTGCG42050.023.0236079
CTATTGA54500.022.9380919
Produced by FastQC (version 0.11.5)