FastQCFastQC Report
Mon 16 Jan 2017
ERR1146578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417925
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.041.3978651
GTATCAA131900.037.1173971
CAACGCA138400.032.2008445
ATCAACG141350.031.6971453
TATCAAC160550.030.1612952
AACGCAG170150.028.2577976
CGTTAAC11550.027.688441
ACGCAGA167550.026.5636757
ACGTTAA9750.026.5116448
CGCAGAG171100.025.9573548
GTACATG289850.025.782331
TACATGG282850.025.6467762
GTGGTAT21700.025.5737151
TATTCGC4850.025.1933639
ACGCAAG8900.024.8191248
GTGATCG30200.024.7439378
ACATGGG285500.024.47013
TATTCTC28850.023.6274665
TGATCGC31550.023.5348959
GTATAAG40400.023.5148141
Produced by FastQC (version 0.11.5)