##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146578_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5417925
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0355453425435	31.0	31.0	34.0	27.0	34.0
2	31.311118924680574	31.0	31.0	34.0	28.0	34.0
3	31.382803194950096	33.0	31.0	34.0	27.0	34.0
4	34.84039055542482	37.0	35.0	37.0	32.0	37.0
5	34.836597405833416	37.0	35.0	37.0	32.0	37.0
6	34.92351795198346	37.0	35.0	37.0	32.0	37.0
7	34.81216019047883	37.0	35.0	37.0	32.0	37.0
8	34.77087685783764	37.0	35.0	37.0	32.0	37.0
9	36.30924828232211	39.0	37.0	39.0	32.0	39.0
10-11	36.3160512557852	39.0	37.0	39.0	32.0	39.0
12-13	36.251555438659636	39.0	37.0	39.0	31.0	39.0
14-15	37.56540603644385	40.0	38.0	41.0	31.0	41.0
16-17	37.62018152706064	40.0	38.0	41.0	31.0	41.0
18-19	37.667211986138604	40.0	38.0	41.0	31.0	41.0
20-21	37.785590885809604	40.0	38.0	41.0	31.0	41.0
22-23	37.830945149665226	40.0	38.0	41.0	31.0	41.0
24-25	37.87720787940032	40.0	38.0	41.0	31.0	41.0
26-27	37.9589943566956	40.0	38.0	41.0	31.0	41.0
28-29	37.96279221288593	40.0	38.0	41.0	31.0	41.0
30-31	37.936031967958215	40.0	38.0	41.0	31.0	41.0
32-33	37.89989636253732	40.0	38.0	41.0	31.0	41.0
34-35	37.84152032374018	40.0	38.0	41.0	31.0	41.0
36-37	37.75478204294079	40.0	37.5	41.0	30.0	41.0
38-39	37.67520757485569	40.0	37.0	41.0	30.0	41.0
40-41	37.59876456392438	40.0	37.0	41.0	30.0	41.0
42-43	37.42138152521491	40.0	37.0	41.0	30.0	41.0
44-45	37.26356437196897	40.0	36.5	41.0	30.0	41.0
46-47	37.134985810988525	40.0	36.0	41.0	30.0	41.0
48-49	36.986599020842846	40.0	35.5	41.0	30.0	41.0
50-51	36.80641389461833	39.0	35.0	41.0	30.0	41.0
52-53	36.633167864080804	39.0	35.0	41.0	30.0	41.0
54-55	36.49421503619928	39.0	35.0	41.0	30.0	41.0
56-57	36.256249578943965	38.5	35.0	41.0	29.5	41.0
58-59	36.012655306228865	38.0	35.0	40.0	29.0	41.0
60-61	35.773157343447906	37.5	35.0	40.0	29.0	41.0
62-63	35.503813637139686	37.0	34.5	40.0	28.5	41.0
64-65	35.19260657908701	36.5	34.0	40.0	28.0	41.0
66-67	34.959878182145374	36.0	34.0	39.5	28.0	41.0
68-69	34.70029328571363	35.5	34.0	39.0	28.0	41.0
70-71	34.47578224135624	35.0	34.0	39.0	28.0	41.0
72-73	34.16855733514215	35.0	34.0	38.0	27.5	40.0
74-75	33.85148917712962	35.0	33.5	37.0	27.0	40.0
76-77	33.607393882344255	35.0	33.0	37.0	27.0	39.0
78-79	33.32074364263072	35.0	33.0	36.0	26.0	39.0
80-81	33.12666906610926	35.0	33.0	36.0	26.0	38.5
82-83	32.860393231726164	35.0	33.0	36.0	26.0	37.0
84-85	32.658325558216475	35.0	33.0	35.0	25.5	37.0
86-87	32.453461795798205	35.0	33.0	35.0	25.0	36.5
88-89	32.29551968696502	35.0	33.0	35.0	25.0	36.0
90-91	32.13829344260026	35.0	33.0	35.0	25.0	36.0
92-93	31.955513872931057	35.0	33.0	35.0	24.5	36.0
94-95	31.832312739655865	35.0	32.0	35.0	24.0	35.5
96-97	31.710053479883904	35.0	32.0	35.0	24.0	35.0
98-99	31.58342649630624	35.0	32.0	35.0	23.0	35.0
100	30.340817010202244	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2870638490332489
1101	2	0.30229196188784613
1101	3	0.36018464337029954
1101	4	0.3140387783523906
1101	5	0.2855897161088521
1101	6	0.27714125908989473
1101	7	0.2840710883058932
1101	8	0.2629314523193784
1101	9	0.3472116422629199
1101	10-11	0.3781008356872064
1101	12-13	0.34426243521014044
1101	14-15	0.4506494487816539
1101	16-17	0.3889911590003621
1101	18-19	0.4313625700296413
1101	20-21	0.36120980724123797
1101	22-23	0.4307345922091983
1101	24-25	0.4635247307137078
1101	26-27	0.44020351816448766
1101	28-29	0.4592852933093212
1101	30-31	0.5136037186343643
1101	32-33	0.5313835432370695
1101	34-35	0.49884734392824015
1101	36-37	0.5207724825911697
1101	38-39	0.5169122617632169
1101	40-41	0.5281624454839715
1101	42-43	0.5221096523014381
1101	44-45	0.44753575463925444
1101	46-47	0.37125807965348656
1101	48-49	0.4237250431062023
1101	50-51	0.4095498350347384
1101	52-53	0.40251480013987617
1101	54-55	0.35844418385397603
1101	56-57	0.44421339883577815
1101	58-59	0.40104958223419374
1101	60-61	0.4414606430315544
1101	62-63	0.35198987656756486
1101	64-65	0.3776671415458708
1101	66-67	0.3705464362910078
1101	68-69	0.3481588701110425
1101	70-71	0.3484560497839624
1101	72-73	0.36946002339998785
1101	74-75	0.3353868513196332
1101	76-77	0.259182349769155
1101	78-79	0.2503655710386141
1101	80-81	0.22123902105413862
1101	82-83	0.2684201585739743
1101	84-85	0.25636358174144647
1101	86-87	0.2186522178833883
1101	88-89	0.20075454568357998
1101	90-91	0.18146069823232835
1101	92-93	0.23297714515989654
1101	94-95	0.2255810942478007
1101	96-97	0.26190406548808554
1101	98-99	0.22484933849575128
1101	100	0.32872233927493255
1102	1	0.20060282004668295
1102	2	0.29893181900251875
1102	3	0.29739000884079303
1102	4	0.3299496511067801
1102	5	0.2786906991266278
1102	6	0.3326103855111242
1102	7	0.3327501218075213
1102	8	0.3568637339747056
1102	9	0.48481476641599386
1102	10-11	0.5074694213647888
1102	12-13	0.5068713772743294
1102	14-15	0.6056989888932449
1102	16-17	0.5544695212946706
1102	18-19	0.5251853145960723
1102	20-21	0.5326731740958834
1102	22-23	0.5178642187718978
1102	24-25	0.5480042739495872
1102	26-27	0.4677300124782704
1102	28-29	0.44529907974444427
1102	30-31	0.5102745676321305
1102	32-33	0.5419629071540157
1102	34-35	0.5273918315941657
1102	36-37	0.4794173419222716
1102	38-39	0.4571471251620878
1102	40-41	0.4245460809756736
1102	42-43	0.5183783852649029
1102	44-45	0.4556604446474495
1102	46-47	0.4697584675726105
1102	48-49	0.4283669752466466
1102	50-51	0.47926071939576786
1102	52-53	0.38093592551518185
1102	54-55	0.35838777570755553
1102	56-57	0.4698557618071817
1102	58-59	0.4191686339576961
1102	60-61	0.4625621940035529
1102	62-63	0.3923092558533696
1102	64-65	0.4604493026452019
1102	66-67	0.39098720333904424
1102	68-69	0.3799968462198322
1102	70-71	0.3136775423989846
1102	72-73	0.3909229192947734
1102	74-75	0.3955435073583118
1102	76-77	0.3416269085500261
1102	78-79	0.3224711500407551
1102	80-81	0.2892956686505599
1102	82-83	0.2999825745139617
1102	84-85	0.3290868187567497
1102	86-87	0.2844745112624949
1102	88-89	0.27355632790466444
1102	90-91	0.2911255106534938
1102	92-93	0.3048823910727556
1102	94-95	0.26608181253942575
1102	96-97	0.32968401443837436
1102	98-99	0.35810410197251485
1102	100	0.38146347938155856
1103	1	0.2523896672917658
1103	2	0.2965810432656184
1103	3	0.23971060612408834
1103	4	0.24718414651646015
1103	5	0.27965791605010537
1103	6	0.29411753407793384
1103	7	0.3319098190202254
1103	8	0.3772950571397473
1103	9	0.40484035191261114
1103	10-11	0.38183510827435896
1103	12-13	0.36394855322551223
1103	14-15	0.46502964226434784
1103	16-17	0.5428068910949619
1103	18-19	0.4730183960956964
1103	20-21	0.4623393373598006
1103	22-23	0.4134997072312814
1103	24-25	0.4526984786584052
1103	26-27	0.41303473676009617
1103	28-29	0.3911442147406845
1103	30-31	0.4900813620814688
1103	32-33	0.46931966986157647
1103	34-35	0.49171443027540107
1103	36-37	0.4420297381154228
1103	38-39	0.47419355775658545
1103	40-41	0.4247344009087257
1103	42-43	0.3989151089847027
1103	44-45	0.3361117617516882
1103	46-47	0.37490797887188876
1103	48-49	0.3413673254374032
1103	50-51	0.323613529048437
1103	52-53	0.33981349560915675
1103	54-55	0.2920238766746408
1103	56-57	0.3269711178712953
1103	58-59	0.36154685589272617
1103	60-61	0.29917305229155033
1103	62-63	0.36734481232421246
1103	64-65	0.36124537359680176
1103	66-67	0.3438491322346451
1103	68-69	0.3326783388699752
1103	70-71	0.324114145744133
1103	72-73	0.3400441424105267
1103	74-75	0.41499390694878
1103	76-77	0.3114837992020867
1103	78-79	0.3097644322371238
1103	80-81	0.21959820809220787
1103	82-83	0.2880589791304473
1103	84-85	0.2883859865323757
1103	86-87	0.2893403913035115
1103	88-89	0.2607534933919027
1103	90-91	0.2793504291977058
1103	92-93	0.20001973256738736
1103	94-95	0.23340759519062004
1103	96-97	0.2737983302527347
1103	98-99	0.2400657218748279
1103	100	0.3500268308328174
1104	1	0.10970791450057504
1104	2	0.1541695315003544
1104	3	0.1460897657678082
1104	4	0.16005671710616554
1104	5	0.17777229403906603
1104	6	0.1883386648087324
1104	7	0.17251566853617106
1104	8	0.24142009439658096
1104	9	0.35027262360784306
1104	10-11	0.3569401586413008
1104	12-13	0.3161651666604399
1104	14-15	0.3931095420921338
1104	16-17	0.36446898824786444
1104	18-19	0.3758888636144633
1104	20-21	0.3399449695703751
1104	22-23	0.36462685994060706
1104	24-25	0.3778607533700722
1104	26-27	0.3596288743960372
1104	28-29	0.40106262563731576
1104	30-31	0.3866509608991535
1104	32-33	0.4113853682184043
1104	34-35	0.4005504504971711
1104	36-37	0.4191446657380169
1104	38-39	0.4237688163831663
1104	40-41	0.4373249231288696
1104	42-43	0.4560616382985856
1104	44-45	0.4239816182673408
1104	46-47	0.4235483758139651
1104	48-49	0.38667302707445117
1104	50-51	0.3660264314962731
1104	52-53	0.36417901687376997
1104	54-55	0.3757999225890529
1104	56-57	0.43724771476517077
1104	58-59	0.3634680076004031
1104	60-61	0.34205629255681913
1104	62-63	0.354898930063527
1104	64-65	0.4223355915714819
1104	66-67	0.3731332734468893
1104	68-69	0.30284547020406194
1104	70-71	0.35898811311746925
1104	72-73	0.3611213364186412
1104	74-75	0.3457175571477933
1104	76-77	0.325685907189758
1104	78-79	0.3463800186843713
1104	80-81	0.3106003034470959
1104	82-83	0.29789521414539166
1104	84-85	0.23814437562311497
1104	86-87	0.22375954267132414
1104	88-89	0.2344213136970481
1104	90-91	0.2577521957772859
1104	92-93	0.28314207692443816
1104	94-95	0.2807338957973329
1104	96-97	0.20889678070935602
1104	98-99	0.2586147626358226
1104	100	0.3259674302044431
1105	1	0.0703357902434405
1105	2	0.11324890496898732
1105	3	0.07848221249036769
1105	4	0.1422915292956901
1105	5	0.0981157817953715
1105	6	0.11082501347365081
1105	7	0.14591886103087148
1105	8	0.14926317027335756
1105	9	0.1703245006581824
1105	10-11	0.17795573493654615
1105	12-13	0.1520522299528153
1105	14-15	0.26959306625199986
1105	16-17	0.2095700766642281
1105	18-19	0.24866034823916294
1105	20-21	0.21923764391403466
1105	22-23	0.20801683604500454
1105	24-25	0.23017011245916308
1105	26-27	0.2133879505733276
1105	28-29	0.1707093285581749
1105	30-31	0.20918409907847746
1105	32-33	0.23304588195409082
1105	34-35	0.1962778593993093
1105	36-37	0.18394036690195747
1105	38-39	0.21510633089780384
1105	40-41	0.21142852499887255
1105	42-43	0.18166491000595641
1105	44-45	0.2057969558701629
1105	46-47	0.18031906567593126
1105	48-49	0.15773868792032175
1105	50-51	0.15930623806446675
1105	52-53	0.15435984187175933
1105	54-55	0.1556532308713301
1105	56-57	0.14530997645167076
1105	58-59	0.0749972405027961
1105	60-61	0.190240842922762
1105	62-63	0.10201410937280997
1105	64-65	0.16665330160039105
1105	66-67	0.165207179132409
1105	68-69	0.1290651608133473
1105	70-71	0.16062553194424822
1105	72-73	0.14940057953564434
1105	74-75	0.17079897749292883
1105	76-77	0.21077158472169089
1105	78-79	0.17532580808938292
1105	80-81	0.10755975934435469
1105	82-83	0.16853533753791083
1105	84-85	0.13582916784471166
1105	86-87	0.1220441303370805
1105	88-89	0.007860323235526323
1105	90-91	0.06202084930686169
1105	92-93	0.14616603710985387
1105	94-95	0.1301843826059894
1105	96-97	0.1289481799794352
1105	98-99	0.11467509211308524
1105	100	0.1434650281230354
1106	1	-0.029208552712972846
1106	2	0.04253130217968604
1106	3	-0.002633831714447865
1106	4	-0.01337704627977132
1106	5	-0.0027616885462506957
1106	6	0.0033149116670259104
1106	7	0.08621792652530758
1106	8	0.09703707225131097
1106	9	0.08793120407847255
1106	10-11	0.07553484891506201
1106	12-13	0.07877457634881324
1106	14-15	0.11627439662930072
1106	16-17	0.028648873050848067
1106	18-19	0.05670709716778788
1106	20-21	0.11157845177041992
1106	22-23	0.1623051889006959
1106	24-25	0.12579342577014074
1106	26-27	0.1318388511331463
1106	28-29	0.11192804476780793
1106	30-31	0.09273115884172256
1106	32-33	0.1443292745240683
1106	34-35	0.11808326066730501
1106	36-37	0.09934566503864062
1106	38-39	0.08453390623854062
1106	40-41	0.0663156488720773
1106	42-43	0.12120253106794365
1106	44-45	0.06628581248704535
1106	46-47	0.10020203828767649
1106	48-49	0.12182649921233946
1106	50-51	0.08166568006149078
1106	52-53	0.07381011782992886
1106	54-55	0.023239759683647776
1106	56-57	0.07917355077704258
1106	58-59	0.10583318611769243
1106	60-61	0.11844734338257723
1106	62-63	0.03636338670779793
1106	64-65	0.13303997273621349
1106	66-67	0.11111722878052888
1106	68-69	0.15584196064208555
1106	70-71	0.14991390519926284
1106	72-73	0.14882692039882528
1106	74-75	0.12962043359888042
1106	76-77	0.1886190715064444
1106	78-79	0.18396977258743163
1106	80-81	0.1394697370848732
1106	82-83	0.14370213862758874
1106	84-85	0.17346509105861685
1106	86-87	0.09885044702452461
1106	88-89	0.12907899067804607
1106	90-91	0.1059222242082285
1106	92-93	0.1145359364353915
1106	94-95	0.0525900099366865
1106	96-97	0.09512756693909452
1106	98-99	0.07628585232048835
1106	100	0.11789717482371742
1107	1	0.03182894942759518
1107	2	0.049914096141527864
1107	3	0.048417323446710014
1107	4	0.03133003846622984
1107	5	0.10502121692717026
1107	6	0.10561261588970439
1107	7	0.0687942107965469
1107	8	0.06515221883714162
1107	9	0.14374071507159414
1107	10-11	0.1762784537041
1107	12-13	0.18059629736286809
1107	14-15	0.1625634198555943
1107	16-17	0.14902146873776445
1107	18-19	0.11675003907205905
1107	20-21	0.20223633436190624
1107	22-23	0.2164735325403555
1107	24-25	0.25965330531401776
1107	26-27	0.2095343359396935
1107	28-29	0.17408599278066816
1107	30-31	0.1699735579195405
1107	32-33	0.1917485179795264
1107	34-35	0.16191177213998031
1107	36-37	0.1489925527994984
1107	38-39	0.11612975569542527
1107	40-41	0.11766437803474616
1107	42-43	0.18217312781340667
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2216	18-19	-0.34626693989614665
2216	20-21	-0.33078129878362716
2216	22-23	-0.3464755499759349
2216	24-25	-0.311757509872713
2216	26-27	-0.41615226916616166
2216	28-29	-0.4323513165879689
2216	30-31	-0.39614946012756747
2216	32-33	-0.34464743000520315
2216	34-35	-0.34143692584270724
2216	36-37	-0.26710355709411715
2216	38-39	-0.28573408751461926
2216	40-41	-0.28138105969165395
2216	42-43	-0.2682179220185432
2216	44-45	-0.25370356701957775
2216	46-47	-0.22489700058304152
2216	48-49	-0.12145099232442647
2216	50-51	-0.2432897187021581
2216	52-53	-0.20090694091808103
2216	54-55	-0.18077374445263672
2216	56-57	-0.128607616628571
2216	58-59	-0.1841823677198704
2216	60-61	-0.19566252982087917
2216	62-63	-0.19078050565958193
2216	64-65	-0.1546641205657764
2216	66-67	-0.13784636905396042
2216	68-69	-0.2309213098863836
2216	70-71	-0.1719392086823106
2216	72-73	-0.1318195440458041
2216	74-75	-0.14233199150100972
2216	76-77	-0.2128495609058234
2216	78-79	-0.13568393518727362
2216	80-81	-0.17713682623176652
2216	82-83	-0.17775798431728873
2216	84-85	-0.18572024209078108
2216	86-87	-0.20369542109312277
2216	88-89	-0.19143462473547856
2216	90-91	-0.18236353713003695
2216	92-93	-0.11842413809804597
2216	94-95	-0.14709844832828622
2216	96-97	-0.14665197961305054
2216	98-99	-0.14746018495557678
2216	100	-0.2550110229398541
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1709.0
1	405.0
2	306.0
3	253.0
4	230.0
5	316.0
6	430.0
7	872.0
8	2834.0
9	3886.0
10	3971.0
11	4630.0
12	6200.0
13	9813.0
14	16209.0
15	18946.0
16	19813.0
17	20908.0
18	22885.0
19	24425.0
20	26422.0
21	29179.0
22	32408.0
23	38614.0
24	49075.0
25	61730.0
26	61883.0
27	53851.0
28	56933.0
29	68519.0
30	78212.0
31	91624.0
32	116704.0
33	156369.0
34	245604.0
35	381290.0
36	717391.0
37	1357304.0
38	1249233.0
39	386167.0
40	372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01327132387894	21.352007113609915	12.913574184380025	23.721147378131118
2	15.57222027472838	21.21426589927611	39.77156328183207	23.441950544163433
3	18.04681180497304	27.769583317708957	30.29581887962961	23.887785997688393
4	12.28482179472496	18.26021258280348	40.45110115232145	29.00386447015011
5	12.907476324412354	39.52481092226634	34.66675730624589	12.900955447075413
6	28.021049959662125	39.29178627780722	19.263399203440144	13.423764559090506
7	25.26899955405355	33.762007209775504	23.9285739356677	17.040419300503252
8	25.10775191206417	35.1550831321404	21.10311799330849	18.63404696248695
9	26.584816951014666	15.494838661772112	21.633862870910576	36.28648151630265
10-11	24.850890427311825	26.990123398637035	27.635318951834307	20.523667222216837
12-13	24.918236173199862	25.540743939539745	27.207974261133355	22.333045626127042
14-15	22.96034425129214	26.191454545941532	25.18983889381629	25.65836230895004
16-17	22.0087211536188	28.501392074338543	26.481415733691154	23.008471038351498
18-19	21.551085490887303	28.89414712446258	27.846722976932654	21.70804440771746
20-21	23.737203209114224	27.677991412931952	26.574953061117057	22.009852316836767
22-23	24.046346364207636	27.44866874359733	26.11110433824342	22.393880553951618
24-25	23.15415485503289	27.728262276040756	26.985525132007215	22.13205773691914
26-27	22.98300336186886	28.14619003896044	26.309232828581997	22.5615737705887
28-29	22.291414653276526	28.651734635661256	26.47705438575572	22.579796325306496
30-31	22.885021117507108	28.035954534782142	26.64238194913584	22.436642398574914
32-33	23.201363410358457	28.440842789478776	25.70170031887849	22.65609348128428
34-35	23.211973510677623	28.141182538713394	26.71663419267034	21.93020975793864
36-37	23.01862400054387	27.87364247527604	26.50051200160205	22.60722152257804
38-39	23.082485897833326	27.697590133970124	26.884196151559635	22.335727816636915
40-41	22.880779501238177	26.989297467951058	27.466916549437904	22.663006481372864
42-43	22.574114459463416	27.56879891156201	26.75371744267323	23.10336918630134
44-45	22.490108518797165	28.324241991791236	26.720138626216777	22.465510863194822
46-47	22.428739191564993	27.672909559490062	26.515573179803848	23.382778069141104
48-49	23.224640867401572	27.12226138069201	26.94730455641517	22.70579319549125
50-51	22.936133284007205	27.19473337332999	26.361251091710113	23.50788225095269
52-53	22.089026606812087	27.872258702445407	26.49451084621021	23.544203844532298
54-55	22.384438292270467	27.701104472461346	26.405751843404552	23.50870539186364
56-57	23.181278755661296	27.1921412793173	26.15210396733557	23.47447599768583
58-59	22.17265797863614	27.375000554272106	27.218640392690702	23.233701074401054
60-61	22.523376805852074	27.737409346546883	26.534063767967066	23.20515007963398
62-63	22.937195573871456	27.18133933593661	26.058447045338507	23.823018044853427
64-65	21.82701069617124	27.42817239806932	26.558484917238257	24.186331988521182
66-67	22.91849506033468	26.838327811969382	26.38537888627961	23.85779824141633
68-69	22.15784089904464	26.85352759878994	26.692417758923327	24.29621374324209
70-71	22.25991684948915	26.90810689766412	26.5681162023031	24.263860050543627
72-73	22.8167621643363	26.888403060827642	26.821548841455268	23.47328593338079
74-75	22.73377687394863	27.285775878434997	26.61136528098178	23.36908196663459
76-77	22.345100022291124	27.157774682660975	26.555064477511507	23.942060817536397
78-79	22.250564083622677	27.19385177259866	26.463617195295758	24.091966948482906
80-81	22.099903600411466	27.205247980666503	27.050777686474543	23.644070732447492
82-83	22.82926791412958	26.25927511184826	27.79886261078468	23.11259436323748
84-85	22.949566003361042	26.755527652094674	26.624040393831727	23.670865950712557
86-87	21.854371992172258	27.154485949675422	27.17795919996216	23.81318285819016
88-89	22.807427892536275	26.107672682538713	26.852111790667642	24.23278763425737
90-91	22.831615167257453	26.00781442550042	26.9655600664677	24.195010340774427
92-93	22.996523822420826	25.9980801213115	27.13643775303251	23.868958303235157
94-95	21.878045700647284	26.22901165567786	27.50246150114297	24.390481142531886
96-97	22.238620111604384	26.341874532776387	27.016357687509025	24.403147668110208
98-99	22.022166026154053	26.69616975642906	26.73000072151785	24.551663495899035
100	22.833214579174548	26.175235992667893	26.136085050057144	24.855464378100415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2471.0
1	1574.0
2	700.0
3	711.5
4	656.0
5	572.0
6	519.0
7	493.0
8	494.0
9	541.0
10	520.5
11	446.5
12	460.0
13	494.0
14	512.5
15	630.5
16	689.5
17	733.0
18	937.5
19	1222.5
20	1605.0
21	2165.5
22	2935.0
23	4056.5
24	5451.5
25	7301.0
26	10501.0
27	13925.0
28	18635.0
29	29348.0
30	43268.5
31	56862.5
32	75450.0
33	93901.5
34	111585.0
35	127584.0
36	140585.0
37	164775.0
38	176588.5
39	171333.5
40	162399.0
41	152241.5
42	152414.0
43	162404.5
44	179004.0
45	209793.0
46	236210.0
47	254614.0
48	267851.0
49	259593.5
50	243497.5
51	240117.5
52	239023.0
53	231112.0
54	220593.0
55	191581.0
56	167609.5
57	136970.5
58	103460.5
59	90897.0
60	71961.5
61	50637.5
62	36403.5
63	28016.0
64	19829.5
65	10626.0
66	6587.5
67	5037.0
68	3630.0
69	2479.0
70	1840.0
71	1683.5
72	1407.0
73	1003.5
74	784.5
75	638.0
76	438.0
77	296.0
78	234.5
79	191.0
80	182.0
81	144.0
82	99.5
83	68.0
84	50.0
85	47.5
86	39.5
87	27.0
88	18.0
89	14.0
90	14.5
91	13.0
92	12.5
93	13.5
94	11.5
95	10.0
96	10.5
97	8.5
98	8.0
99	7.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09601461814255458
2	0.06568935524208991
3	0.08076892906417124
4	0.08314991440449988
5	0.08385128993110831
6	0.0904036139296871
7	0.08709976605434737
8	0.08852097435826446
9	0.07417969056419202
10-11	0.08070432868672046
12-13	0.07117115870005583
14-15	0.07976300890100915
16-17	0.08159950534568124
18-19	0.08133187521052801
20-21	0.07327528527988113
22-23	0.07691136366782486
24-25	0.07937540663630449
26-27	0.07331219978128158
28-29	0.08194096448363533
30-31	0.08571547225183072
32-33	0.08738585344020082
34-35	0.08999755441428221
36-37	0.08962840940027778
38-39	0.09093887419999354
40-41	0.0871182233050476
42-43	0.0928491996474665
44-45	0.08873323274131702
46-47	0.08630610427423784
48-49	0.09525787086384548
50-51	0.09177867910685364
52-53	0.09691902342686545
54-55	0.08938846514117489
56-57	0.09572853075670114
58-59	0.10005675604590318
60-61	0.0987186053701371
62-63	0.09938306639534508
64-65	0.10190248111592538
66-67	0.10363746268174624
68-69	0.10093347545416373
70-71	0.10282534365093647
72-73	0.09970606828259897
74-75	0.10416349432670258
76-77	0.10071198844576106
78-79	0.10757808570624364
80-81	0.10257617076648348
82-83	0.10693208193173587
84-85	0.10360977680569591
86-87	0.0993184660178943
88-89	0.09831254585473222
90-91	0.0991338935108921
92-93	0.09381820530922816
94-95	0.09356903242477517
96-97	0.09164947835195209
98-99	0.10564007438272033
100	0.24367262374433019
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5417925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.042860031837066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.08050902394596	36.471901220931144
2	14.238300651934757	14.8200377583971
3	5.89160606400409	9.198480892550622
4	3.075631982572747	6.402587391139002
5	1.8574178553458922	4.8332668733200626
6	1.1917865525234168	3.7214388444481092
7	0.8229659503361995	2.9980651235020224
8	0.5685645207901985	2.3671779019642254
9	0.42713497171397946	2.0006392992851985
>10	1.7967835099112932	15.105471800567216
>50	0.042724300613671475	1.4562223175873241
>100	0.006432462092843096	0.5095219738295259
>500	7.10771074843113E-5	0.027668938552928415
>1k	7.107710748430853E-5	0.08751966392543593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.8457250700221948E-5	0.0
14-15	0.0	0.0	0.0	1.8457250700221948E-5	0.0
16-17	0.0	0.0	0.0	2.768587605033292E-5	0.0
18-19	0.0	0.0	0.0	3.6914501400443896E-5	0.0
20-21	0.0	0.0	0.0	8.305762815099877E-5	0.0
22-23	0.0	0.0	0.0	2.9531601120355116E-4	0.0
24-25	0.0	0.0	0.0	0.0010151487885122071	0.0
26-27	0.0	0.0	0.0	0.0024640429684796303	0.0
28-29	0.0	0.0	0.0	0.008379591817900765	0.0
30-31	0.0	0.0	0.0	0.02803656381363714	0.0
32-33	0.0	0.0	0.0	0.0718171624745636	0.0
34-35	0.0	0.0	0.0	0.14223157389591035	0.0
36-37	0.0	0.0	0.0	0.25298430672259214	0.0
38-39	0.0	0.0	0.0	0.41360853094127364	0.0
40-41	0.0	0.0	0.0	0.5939174130317418	0.0
42-43	0.0	0.0	0.0	0.7802895019772329	0.0
44-45	0.0	0.0	0.0	1.0119279982650184	0.0
46-47	0.0	0.0	0.0	1.284070931214441	0.0
48-49	0.0	0.0	0.0	1.5892984860440114	0.0
50-51	0.0	0.0	0.0	1.912918691196353	0.0
52-53	0.0	0.0	0.0	2.234010253002764	0.0
54-55	0.0	0.0	0.0	2.5786717239533585	0.0
56-57	0.0	0.0	0.0	2.989050236022093	0.0
58-59	0.0	0.0	0.0	3.4581781770696347	0.0
60-61	0.0	0.0	0.0	3.9442406456346295	0.0
62-63	0.0	0.0	0.0	4.444081451847341	0.0
64-65	0.0	0.0	0.0	4.972447939017243	0.0
66-67	0.0	0.0	0.0	5.556186178287813	0.0
68-69	0.0	0.0	0.0	6.193690388848129	0.0
70-71	0.0	0.0	0.0	6.889076537604341	0.0
72-73	0.0	0.0	0.0	7.598582483146223	0.0
74-75	0.0	0.0	0.0	8.309251235482218	0.0
76-77	0.0	0.0	0.0	9.10025517149093	0.0
78-79	0.0	0.0	0.0	9.934818219152167	0.0
80-81	0.0	0.0	0.0	10.839408075970043	0.0
82-83	0.0	0.0	0.0	11.77628889288796	0.0
84-85	0.0	0.0	0.0	12.698385451994998	0.0
86-87	0.0	0.0	0.0	13.653511261230083	0.0
88	0.0	0.0	0.0	14.39912881776695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7975	0.0	41.397865	1
GTATCAA	13190	0.0	37.117397	1
CAACGCA	13840	0.0	32.200844	5
ATCAACG	14135	0.0	31.697145	3
TATCAAC	16055	0.0	30.161295	2
AACGCAG	17015	0.0	28.257797	6
CGTTAAC	1155	0.0	27.68844	1
ACGCAGA	16755	0.0	26.563675	7
ACGTTAA	975	0.0	26.511644	8
CGCAGAG	17110	0.0	25.957354	8
GTACATG	28985	0.0	25.78233	1
TACATGG	28285	0.0	25.646776	2
GTGGTAT	2170	0.0	25.573715	1
TATTCGC	485	0.0	25.193363	9
ACGCAAG	890	0.0	24.819124	8
GTGATCG	3020	0.0	24.743937	8
ACATGGG	28550	0.0	24.4701	3
TATTCTC	2885	0.0	23.627466	5
TGATCGC	3155	0.0	23.534895	9
GTATAAG	4040	0.0	23.514814	1
>>END_MODULE