FastQCFastQC Report
Mon 16 Jan 2017
ERR1146578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417925
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217200.4008914852088207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212170.3916074881066091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108340.19996585408620457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142000.059.4766041
GTATCAA263250.043.586261
GTGGTAT28200.041.5220151
CAACGCA329450.034.3677375
ATCAACG330700.034.1831743
TATCAAC339700.033.721112
AACGCAG367900.031.5543176
TCAACGC393050.029.0475164
ACGCAGA390500.028.957317
CGCAGAG393900.028.67138
GCAGAGT416550.026.8640739
TGGTATC45850.025.7427252
TAGGCAT54800.025.044135
GTACATG273100.024.4853361
GTGATCG30150.024.4732678
TACATGG269850.024.4661062
GTACTAG38150.024.15961
GAGTACT270750.023.9544512-13
ACATGGG267450.023.8236793
GTATAAG39500.023.0957891
Produced by FastQC (version 0.11.5)