##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5417925
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24319033578353	33.0	31.0	34.0	30.0	34.0
2	32.370991846509504	34.0	31.0	34.0	30.0	34.0
3	32.455806420354655	34.0	31.0	34.0	30.0	34.0
4	35.98126016879156	37.0	35.0	37.0	35.0	37.0
5	36.047129851372986	37.0	35.0	37.0	35.0	37.0
6	36.121841110757344	37.0	36.0	37.0	35.0	37.0
7	36.09046230798691	37.0	36.0	37.0	35.0	37.0
8	36.10132587660405	37.0	36.0	37.0	35.0	37.0
9	37.87403018683352	39.0	38.0	39.0	35.0	39.0
10-11	37.82137700318849	39.0	38.0	39.0	35.0	39.0
12-13	37.78511551931782	39.0	38.0	39.0	35.0	39.0
14-15	39.08279101316464	40.0	38.5	41.0	36.0	41.0
16-17	39.02777973117014	40.0	38.5	41.0	35.5	41.0
18-19	39.14886243349622	40.0	39.0	41.0	36.0	41.0
20-21	39.21249888102918	40.0	39.0	41.0	36.0	41.0
22-23	39.17030126847455	40.0	39.0	41.0	35.5	41.0
24-25	39.134344790671705	40.5	39.0	41.0	35.0	41.0
26-27	39.032837110148265	40.0	39.0	41.0	35.0	41.0
28-29	38.97651850108667	40.0	38.5	41.0	35.0	41.0
30-31	38.80035631722477	40.0	38.0	41.0	35.0	41.0
32-33	38.64907376901674	40.0	38.0	41.0	35.0	41.0
34-35	38.550146227568675	40.0	38.0	41.0	35.0	41.0
36-37	38.46413876161077	40.0	38.0	41.0	35.0	41.0
38-39	38.34938449683227	40.0	38.0	41.0	34.0	41.0
40-41	38.20804976074788	40.0	38.0	41.0	34.0	41.0
42-43	38.05363861625992	40.0	37.0	41.0	33.5	41.0
44-45	37.80486007096813	40.0	37.0	41.0	33.0	41.0
46-47	37.4391275257594	40.0	37.0	41.0	32.5	41.0
48-49	37.02366542541655	40.0	36.0	41.0	31.0	41.0
50-51	36.82922290360239	40.0	36.0	41.0	31.0	41.0
52-53	36.68015448718836	39.5	35.0	41.0	31.0	41.0
54-55	36.49018461865013	39.0	35.0	41.0	30.5	41.0
56-57	36.26603958157413	39.0	35.0	41.0	30.0	41.0
58-59	36.02497515192624	38.5	35.0	41.0	30.0	41.0
60-61	35.79887253884098	37.5	35.0	40.5	30.0	41.0
62-63	35.52919124941744	37.0	35.0	40.0	29.0	41.0
64-65	35.254512013363055	37.0	35.0	40.0	29.0	41.0
66-67	35.00227697873264	36.0	35.0	39.5	29.0	41.0
68-69	34.74201580863522	36.0	35.0	39.0	29.0	41.0
70-71	34.504978843376385	35.0	34.0	39.0	29.0	41.0
72-73	34.235236275880524	35.0	34.0	38.5	28.5	40.5
74-75	33.9574218912222	35.0	34.0	37.0	28.0	40.0
76-77	33.70762708970685	35.0	34.0	37.0	28.0	39.0
78-79	33.46791234651643	35.0	34.0	36.0	27.0	39.0
80-81	33.27722772094483	35.0	34.0	36.0	27.0	38.5
82-83	33.061090085226354	35.0	34.0	36.0	27.0	37.0
84-85	32.88468795710535	35.0	34.0	35.0	27.0	37.0
86-87	32.73290318710576	35.0	34.0	35.0	27.0	36.5
88-89	32.57631454846643	35.0	34.0	35.0	26.0	36.0
90-91	32.501649616781336	35.0	34.0	35.0	26.5	36.0
92-93	32.40786334251582	35.0	34.0	35.0	26.0	36.0
94-95	32.36988016629983	35.0	34.0	35.0	26.0	35.5
96-97	32.31634620265138	35.0	33.5	35.0	25.5	35.0
98-99	32.24976416247918	35.0	33.0	35.0	25.0	35.0
100	31.477328682106158	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35411581976288176
1101	2	0.3728139403401016
1101	3	0.3706494629705972
1101	4	0.2847599249204791
1101	5	0.27110667103696073
1101	6	0.2650089250154508
1101	7	0.27706726575529217
1101	8	0.26842022878906135
1101	9	0.3129343000935023
1101	10-11	0.3387748040037195
1101	12-13	0.3662611962255724
1101	14-15	0.5823744289482917
1101	16-17	0.5836416782419747
1101	18-19	0.5258297491841546
1101	20-21	0.4743948944124128
1101	22-23	0.48218692958799636
1101	24-25	0.5075468975924267
1101	26-27	0.499281692196206
1101	28-29	0.5791634410531756
1101	30-31	0.5885117869001917
1101	32-33	0.5147844389384204
1101	34-35	0.47713407503052707
1101	36-37	0.503036510574475
1101	38-39	0.4722616731908147
1101	40-41	0.5057923017096826
1101	42-43	0.5383435858760777
1101	44-45	0.5182752706007037
1101	46-47	0.52361066485593
1101	48-49	0.571542349909258
1101	50-51	0.5599488616193327
1101	52-53	0.44453069229891895
1101	54-55	0.4894960489164859
1101	56-57	0.41018347635329633
1101	58-59	0.46524105113182657
1101	60-61	0.4166662514754691
1101	62-63	0.4337762089106576
1101	64-65	0.46086313798542733
1101	66-67	0.4162704877348986
1101	68-69	0.42192904473295556
1101	70-71	0.3977431526603752
1101	72-73	0.4179483784594993
1101	74-75	0.3943576909032416
1101	76-77	0.3335763454806866
1101	78-79	0.33120482126697226
1101	80-81	0.3052744877385578
1101	82-83	0.34689012966885713
1101	84-85	0.32371326584611637
1101	86-87	0.3241465890213675
1101	88-89	0.39964572504931795
1101	90-91	0.3439039876915899
1101	92-93	0.32230905698209256
1101	94-95	0.3439705118510048
1101	96-97	0.29886001683939156
1101	98-99	0.3783937189727524
1101	100	0.4132143206701073
1102	1	0.31984545276333165
1102	2	0.290211395934719
1102	3	0.31513818369611357
1102	4	0.179777601678623
1102	5	0.1796567565083862
1102	6	0.23398140854362737
1102	7	0.22249420857465907
1102	8	0.21750250123341885
1102	9	0.2957353521321906
1102	10-11	0.28183545573198643
1102	12-13	0.29150013342719916
1102	14-15	0.43537611299954193
1102	16-17	0.521283615714772
1102	18-19	0.4311457319152652
1102	20-21	0.3930495500977429
1102	22-23	0.44066634967371954
1102	24-25	0.4383950445269207
1102	26-27	0.42226102433698287
1102	28-29	0.45076139344426736
1102	30-31	0.5109956867872043
1102	32-33	0.490727011572595
1102	34-35	0.5029824342861815
1102	36-37	0.5548985612948272
1102	38-39	0.5394560361049372
1102	40-41	0.5496283012661181
1102	42-43	0.5930424302067578
1102	44-45	0.6025190030688847
1102	46-47	0.6435131520338473
1102	48-49	0.6599735966400289
1102	50-51	0.728138687710981
1102	52-53	0.6218882969052331
1102	54-55	0.6093030791483329
1102	56-57	0.6266779131302584
1102	58-59	0.587631121024998
1102	60-61	0.5916865134529203
1102	62-63	0.48897024223072094
1102	64-65	0.5193558850674123
1102	66-67	0.5217828062348531
1102	68-69	0.46681485662356437
1102	70-71	0.5202082550215508
1102	72-73	0.5244773766839046
1102	74-75	0.49553999086177924
1102	76-77	0.5065049424005892
1102	78-79	0.4540678847067028
1102	80-81	0.4696884710271121
1102	82-83	0.3949658382951071
1102	84-85	0.3903692749377399
1102	86-87	0.4283688180852607
1102	88-89	0.48869660510008117
1102	90-91	0.4550106767967037
1102	92-93	0.4766490824474019
1102	94-95	0.4979370583658209
1102	96-97	0.4963315579224101
1102	98-99	0.4706384658339715
1102	100	0.5386339663362563
1103	1	0.3003931815060241
1103	2	0.27240592681456377
1103	3	0.2731155954754243
1103	4	0.22126254887370322
1103	5	0.1965377545507181
1103	6	0.224372403338144
1103	7	0.22587519511285592
1103	8	0.23673576856497647
1103	9	0.2747616804158639
1103	10-11	0.3084712126715843
1103	12-13	0.2809915559845564
1103	14-15	0.42017002990839103
1103	16-17	0.48331724147620037
1103	18-19	0.3774620259529158
1103	20-21	0.350173982800726
1103	22-23	0.3839125996426844
1103	24-25	0.3687512951350982
1103	26-27	0.4023106817378235
1103	28-29	0.3883731700022679
1103	30-31	0.4076365911887194
1103	32-33	0.4144461047050214
1103	34-35	0.41587849104147523
1103	36-37	0.43963229938839987
1103	38-39	0.4268462366132013
1103	40-41	0.3981198956576151
1103	42-43	0.4404833294432251
1103	44-45	0.4156188522030888
1103	46-47	0.4582888046883298
1103	48-49	0.5643148125455113
1103	50-51	0.47910521930190697
1103	52-53	0.4582555549184164
1103	54-55	0.477865020232457
1103	56-57	0.49155148893595424
1103	58-59	0.4700492102198979
1103	60-61	0.426233083917424
1103	62-63	0.45887220413270313
1103	64-65	0.5142804350577279
1103	66-67	0.4410236339660827
1103	68-69	0.43840839887386807
1103	70-71	0.40526585764811074
1103	72-73	0.43816859880653425
1103	74-75	0.4551414328317165
1103	76-77	0.4423817310642022
1103	78-79	0.3782971677511142
1103	80-81	0.43105201170199337
1103	82-83	0.4158946939888182
1103	84-85	0.389598904429576
1103	86-87	0.4413544142477335
1103	88-89	0.48245486087598266
1103	90-91	0.3959962840757356
1103	92-93	0.45461594331935373
1103	94-95	0.488498781315684
1103	96-97	0.4336933629760509
1103	98-99	0.44598184532960516
1103	100	0.4696337394864578
1104	1	0.27147915027254754
1104	2	0.26201991460491314
1104	3	0.25212102595628494
1104	4	0.2385875646691673
1104	5	0.1555631498499963
1104	6	0.17345456181386965
1104	7	0.2174163100598534
1104	8	0.20341853126087273
1104	9	0.25370985066340523
1104	10-11	0.23496720580106256
1104	12-13	0.265150174187994
1104	14-15	0.38263622510674367
1104	16-17	0.45659735742503216
1104	18-19	0.35635969199278605
1104	20-21	0.3570891025348999
1104	22-23	0.39774955027596803
1104	24-25	0.3659719756436317
1104	26-27	0.39018048442059694
1104	28-29	0.35449623231040306
1104	30-31	0.40380937892228985
1104	32-33	0.42769197038518314
1104	34-35	0.37731073617915456
1104	36-37	0.364265576394601
1104	38-39	0.43649639830023546
1104	40-41	0.4483602684107879
1104	42-43	0.446743949099087
1104	44-45	0.4372269998444125
1104	46-47	0.45212289500158676
1104	48-49	0.5628900358218445
1104	50-51	0.39311950439327603
1104	52-53	0.38728861960776584
1104	54-55	0.37258942552724506
1104	56-57	0.34558800341528695
1104	58-59	0.3635992513751347
1104	60-61	0.32125460181425325
1104	62-63	0.32175687814683585
1104	64-65	0.33509198177847566
1104	66-67	0.32192791140062127
1104	68-69	0.2970435955603463
1104	70-71	0.32847583833467553
1104	72-73	0.346240631857043
1104	74-75	0.4052547689751336
1104	76-77	0.35445468609239583
1104	78-79	0.393939385859305
1104	80-81	0.3306953509695063
1104	82-83	0.27784218253272996
1104	84-85	0.30578158664532396
1104	86-87	0.28339732335469137
1104	88-89	0.3471458700503618
1104	90-91	0.3046481958694969
1104	92-93	0.3655341832068899
1104	94-95	0.34224509174509876
1104	96-97	0.31072287577239877
1104	98-99	0.3442851318444866
1104	100	0.38601016621462136
1105	1	0.2093074841572502
1105	2	0.17793675184461932
1105	3	0.16326035722209298
1105	4	0.13300112730187408
1105	5	0.09934271456253185
1105	6	0.14228487008428203
1105	7	0.15588124494335176
1105	8	0.14927462780279654
1105	9	0.16214530815282302
1105	10-11	0.1623242934898954
1105	12-13	0.1688251116152628
1105	14-15	0.3171731637808506
1105	16-17	0.34435858789699836
1105	18-19	0.2622625681640116
1105	20-21	0.23965340187181283
1105	22-23	0.23370407943506422
1105	24-25	0.23220474095665367
1105	26-27	0.24589873971210352
1105	28-29	0.23422073762661455
1105	30-31	0.2642859466016745
1105	32-33	0.2643226409777668
1105	34-35	0.23317711982417677
1105	36-37	0.27833585885828427
1105	38-39	0.2838027061857815
1105	40-41	0.23341650479405018
1105	42-43	0.27236315230660324
1105	44-45	0.284582239017908
1105	46-47	0.21863148779706165
1105	48-49	0.3281406167367251
1105	50-51	0.2782181973183029
1105	52-53	0.23985247081974848
1105	54-55	0.24313615111794462
1105	56-57	0.21654038091573113
1105	58-59	0.21792023007996875
1105	60-61	0.24876657268085722
1105	62-63	0.20744357028632976
1105	64-65	0.22457636791982338
1105	66-67	0.19465672530620282
1105	68-69	0.16893693655573117
1105	70-71	0.18459384742106266
1105	72-73	0.21847624474037275
1105	74-75	0.23526040678211046
1105	76-77	0.16541363060003533
1105	78-79	0.14336975647179884
1105	80-81	0.22641878935299076
1105	82-83	0.1680724001237479
1105	84-85	0.21293698846181286
1105	86-87	0.18381004311713411
1105	88-89	0.23122751709399125
1105	90-91	0.23238118129094687
1105	92-93	0.2225003181425791
1105	94-95	0.21542549036540493
1105	96-97	0.1986896011785788
1105	98-99	0.27342933185582297
1105	100	0.25518065402496504
1106	1	0.1289402224636902
1106	2	0.08201665075069542
1106	3	0.11830707867537882
1106	4	0.10233449603652645
1106	5	0.060804575676570494
1106	6	0.09712595683761549
1106	7	0.10617639121858247
1106	8	0.11276666741471075
1106	9	0.11915458754814523
1106	10-11	0.11307296603606432
1106	12-13	0.15776354092378142
1106	14-15	0.28957203224335615
1106	16-17	0.3267318469266627
1106	18-19	0.2493826690921921
1106	20-21	0.21950221327359998
1106	22-23	0.17139551429639965
1106	24-25	0.19260534342505053
1106	26-27	0.24903863320724895
1106	28-29	0.21964710539140953
1106	30-31	0.19883001352980045
1106	32-33	0.1827752228877273
1106	34-35	0.18343846378997597
1106	36-37	0.2281144322166
1106	38-39	0.25273788977136746
1106	40-41	0.24913833183612866
1106	42-43	0.21203753646071988
1106	44-45	0.2101618729240755
1106	46-47	0.19781054014413257
1106	48-49	0.23715680028164599
1106	50-51	0.24370138579309497
1106	52-53	0.2368826088377176
1106	54-55	0.2130459759273151
1106	56-57	0.22304267985525428
1106	58-59	0.25796326499379063
1106	60-61	0.254363062162021
1106	62-63	0.22958202709568098
1106	64-65	0.25181059060822264
1106	66-67	0.17263163611973908
1106	68-69	0.17245970774205688
1106	70-71	0.2116310015298879
1106	72-73	0.20326060768047682
1106	74-75	0.21992020637920007
1106	76-77	0.14909317916055187
1106	78-79	0.16994736810420363
1106	80-81	0.14631742003593473
1106	82-83	0.14668661444587627
1106	84-85	0.1589734740467037
1106	86-87	0.1782051309483137
1106	88-89	0.16975909313647009
1106	90-91	0.13473536950704101
1106	92-93	0.1680097681909558
1106	94-95	0.20327196642763568
1106	96-97	0.09836476114430326
1106	98-99	0.14971954098581364
1106	100	0.19478510676286476
1107	1	0.11435318360277336
1107	2	0.08409195339057618
1107	3	0.08579470844200898
1107	4	0.07106991802612583
1107	5	0.06800383239034602
1107	6	0.12461713583017087
1107	7	0.1074432659697564
1107	8	0.08292288308208384
1107	9	0.11621479043831329
1107	10-11	0.1306604449761366
1107	12-13	0.14262937894046246
1107	14-15	0.22251924481122387
1107	16-17	0.27073708156872556
1107	18-19	0.18589038928016777
1107	20-21	0.18174363957759
1107	22-23	0.1922542715762745
1107	24-25	0.19206298316493786
1107	26-27	0.20560061428447796
1107	28-29	0.2130253360183616
1107	30-31	0.22787708677034857
1107	32-33	0.22163219187986272
1107	34-35	0.1584193501827329
1107	36-37	0.19484262242527706
1107	38-39	0.16744139732031016
1107	40-41	0.12985113263846415
1107	42-43	0.15827884559674033
1107	44-45	0.16358379135217405
1107	46-47	0.27448529616592765
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2216	66-67	-0.9647469141237934
2216	68-69	-0.8888791748218736
2216	70-71	-0.842870076541459
2216	72-73	-0.8390749623927292
2216	74-75	-0.8692218317394733
2216	76-77	-0.8418193455021878
2216	78-79	-0.8083069186203602
2216	80-81	-0.8243772547156709
2216	82-83	-0.8769541930336473
2216	84-85	-0.8128175326265392
2216	86-87	-0.7686035836326788
2216	88-89	-0.6926066421563348
2216	90-91	-0.7336241717246281
2216	92-93	-0.7062419601135943
2216	94-95	-0.7168421004505205
2216	96-97	-0.7162027556578181
2216	98-99	-0.7912702015269346
2216	100	-0.9859290672794181
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	41.0
8	190.0
9	462.0
10	562.0
11	331.0
12	365.0
13	510.0
14	1079.0
15	2025.0
16	4220.0
17	7501.0
18	13409.0
19	19630.0
20	27787.0
21	42346.0
22	59827.0
23	61394.0
24	48116.0
25	35699.0
26	31361.0
27	35850.0
28	45500.0
29	55424.0
30	68909.0
31	87011.0
32	111979.0
33	148657.0
34	221879.0
35	354018.0
36	638798.0
37	1411079.0
38	1408068.0
39	473211.0
40	684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18729927158195	21.11960128229928	13.415598453089908	23.277500993028863
2	15.869730656516781	21.35341210311553	39.49712139533004	23.279735845037653
3	17.81255335308878	28.008560488675073	31.143087331565738	23.035798826670405
4	12.290859533983594	18.6350239877679	39.846323168232	29.22779331001651
5	12.26770039728391	39.01871021900451	35.06805729570814	13.645532088003442
6	27.465023357753964	39.64356006539322	18.612666254067463	14.278750322785353
7	24.200620791399214	34.48218071759233	23.99698186925137	17.320216621757083
8	25.66006602182947	33.84749271166623	21.011664102637457	19.480777163866843
9	26.975706373087988	13.937019776766855	21.991896156516066	37.09537769362909
10-11	25.037435960190617	26.154802240491232	27.745579068273262	21.06218273104489
12-13	25.743305785886662	24.924671345579718	27.82857828412169	21.50344458441193
14-15	23.273060543680668	25.109825296520455	26.89323011788835	24.723884041910527
16-17	21.17028717441663	27.587790087671273	28.094452297514383	23.147470440397722
18-19	20.55780821402016	26.817886459922484	31.35513549066205	21.269169835395303
20-21	22.529680052618108	25.639931216974677	30.627254272475906	21.203134457931306
22-23	22.76680648034072	25.425804159341446	30.335478988727232	21.4719103715906
24-25	22.002057983453074	25.70662200011997	31.15493477669034	21.136385239736615
26-27	21.889445151154284	26.11518135782298	30.420044046521276	21.575329444501456
28-29	21.2141733228127	26.517698196265176	30.680241974556683	21.587886506365443
30-31	21.87380402551572	25.942408282744218	30.732830870623744	21.450956821116318
32-33	22.303528694969895	26.25609563984042	29.775709616412012	21.66466604877767
34-35	22.334793118907832	25.99184299359219	30.593093030737446	21.08027085676253
36-37	22.154673749335537	25.807320890673914	30.29168020199634	21.74632515799421
38-39	22.230353746854277	25.547200593443385	30.727574472742663	21.494871186959678
40-41	22.040626328642116	24.985711689817283	31.036332314209748	21.937329667330854
42-43	22.000433745391454	25.487202203795732	30.09500869797939	22.417355352833418
44-45	21.959047006175552	26.348510477640346	29.76975649858077	21.922686017603333
46-47	22.205724516304674	25.816175011651136	29.00945472667119	22.968645745373
48-49	23.26226240655779	25.68465837726316	28.706186376656284	22.346892839522763
50-51	22.804653072901527	25.94373307124037	27.911580540520585	23.340033315337514
52-53	21.92540581206526	26.630880366606853	28.031847013844104	23.411866807483783
54-55	22.269310948541083	26.574231790169062	27.7471945103499	23.409262750939963
56-57	23.004799166912687	26.077943756824002	27.444889131335376	23.47236794492794
58-59	21.88477262042951	26.245445865439226	28.59858073611463	23.271200778016638
60-61	22.31656398371285	26.70535700195491	27.80589604016545	23.17218297416679
62-63	22.622300276028692	26.13585669025815	27.382424788871734	23.859418244841418
64-65	21.50789239349184	26.384628453813296	27.86641851423608	24.241060638458784
66-67	22.47842922569286	25.871510593752557	27.740485501386804	23.909574679167783
68-69	21.814898028211648	25.715145315047305	28.119609490757085	24.350347165983962
70-71	21.92473536459965	25.739215469484765	27.946363952329172	24.38968521358641
72-73	22.418862476688943	25.68439013049947	28.1677128090633	23.729034583748287
74-75	22.28807534746009	26.212265336330994	27.758913451735445	23.74074586447347
76-77	22.015244118533044	26.02721710483571	27.531611223664083	24.425927552967167
78-79	21.727983323301693	26.221741835658698	27.441190329980902	24.609084511058704
80-81	21.47866097882045	26.34463408853815	27.956301260938805	24.220403671702595
82-83	22.144835575704448	25.47838516794873	28.595937328721327	23.780841927625495
84-85	22.304716274703534	25.942916248531027	27.33060361590351	24.42176386086193
86-87	21.126501381986646	26.449489426302502	27.796914870545457	24.62709432116539
88-89	21.869175007036826	25.550778203832646	27.549661540165282	25.030385248965242
90-91	21.699669624349625	25.49820978461582	27.637617991468506	25.164502599566053
92-93	21.76679426791093	25.65311041875849	27.67804803319355	24.902047280137026
94-95	20.721488858780994	25.804415955454502	28.070995491536742	25.40309969422776
96-97	21.078226532538586	25.83837741001782	27.6086831332991	25.474712924144495
98-99	20.868685528204868	26.174539442847223	27.362624209302407	25.594150819645506
100	21.508124956708457	25.62896261919605	26.87169541225093	25.991217011844565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	168.0
2	178.5
3	298.0
4	211.0
5	172.0
6	373.5
7	869.5
8	1599.0
9	2027.5
10	1715.5
11	1365.5
12	1542.5
13	1862.5
14	2360.5
15	3105.0
16	3925.0
17	4903.5
18	6124.0
19	7391.0
20	8782.0
21	10276.5
22	11746.0
23	13213.0
24	14637.0
25	16360.5
26	19307.0
27	22303.0
28	26378.0
29	36059.5
30	48725.5
31	61027.0
32	78105.0
33	94830.0
34	109810.0
35	124037.0
36	135634.0
37	157961.0
38	169848.0
39	166165.5
40	158806.0
41	149697.0
42	149537.0
43	157884.0
44	173779.0
45	204671.0
46	231542.5
47	251063.0
48	265373.0
49	256024.0
50	238532.0
51	235053.5
52	232598.0
53	223453.0
54	215784.5
55	188438.0
56	164042.0
57	133918.0
58	100797.0
59	89622.5
60	70660.5
61	48709.5
62	34540.0
63	26608.5
64	18794.0
65	9625.0
66	5774.5
67	4361.0
68	3079.0
69	2102.5
70	1587.5
71	1417.5
72	1205.0
73	880.0
74	678.0
75	569.0
76	364.0
77	221.5
78	189.5
79	129.0
80	96.5
81	77.5
82	49.0
83	32.5
84	14.0
85	6.5
86	4.5
87	2.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002897788359934846
2	0.003617621137243502
3	1.8457250700221948E-4
4	0.0491885731160915
5	0.002528643345930407
6	0.004392825666652824
7	3.69145014004439E-5
8	3.6914501400443897E-4
9	3.69145014004439E-5
10-11	1.107435042013317E-4
12-13	0.0
14-15	3.69145014004439E-5
16-17	2.8608738585344016E-4
18-19	1.0151487885122071E-4
20-21	3.4145913795410605E-4
22-23	0.0
24-25	0.0
26-27	3.1377326190377313E-4
28-29	0.0
30-31	1.9380113235233047E-4
32-33	2.8608738585344016E-4
34-35	1.107435042013317E-4
36-37	9.228625350110974E-5
38-39	9.874629124618742E-4
40-41	6.367751491576573E-4
42-43	0.0
44-45	5.629461463567694E-4
46-47	0.0
48-49	7.38290028008878E-5
50-51	0.0
52-53	4.245167661051048E-4
54-55	3.783736393545499E-4
56-57	1.0151487885122071E-4
58-59	8.490335322102096E-4
60-61	6.367751491576573E-4
62-63	1.8457250700221948E-4
64-65	3.4145913795410605E-4
66-67	0.0011443495434137606
68-69	0.002353299464278298
70-71	0.003469963131641726
72-73	0.0030177604894862887
74-75	0.0026670727261820714
76-77	0.004226710410350826
78-79	0.0012181785462146487
80-81	0.001052063289912651
82-83	6.736896505581011E-4
84-85	4.7988851820577065E-4
86-87	0.0
88-89	0.0
90-91	2.7685876050332923E-5
92-93	9.228625350110974E-5
94-95	6.460037745077682E-5
96-97	3.50687763304217E-4
98-99	6.460037745077682E-5
100	0.07475186533589889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5417925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.0670112261503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.57199059347927	32.96050641656241
2	14.725257889032473	14.15598272520164
3	6.2752872520471445	9.049029083744038
4	3.329071302114888	6.400740306056445
5	2.0027994288498814	4.813429131512732
6	1.2615025819515988	3.638199526109106
7	0.8169881104088123	2.7489123672256186
8	0.5829236347871847	2.241551751784315
9	0.4472009125869342	1.934605015709474
>10	1.9150473355451745	14.962428719865336
>50	0.050460290705585366	1.6119759743815658
>100	0.017720252477607154	1.6214754108855798
>500	0.002059051929068513	0.6646001507392402
>1k	0.0014707513777559257	1.2959031339466525
>5k	0.0	0.0
>10k+	2.2061270666338885E-4	1.9006602862758564
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21720	0.4008914852088207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21217	0.3916074881066091	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10834	0.19996585408620457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.8457250700221948E-5	0.0
14-15	0.0	0.0	0.0	1.8457250700221948E-5	0.0
16-17	0.0	0.0	0.0	2.768587605033292E-5	0.0
18-19	0.0	0.0	0.0	3.6914501400443896E-5	0.0
20-21	0.0	0.0	0.0	8.305762815099877E-5	0.0
22-23	0.0	0.0	0.0	2.9531601120355116E-4	0.0
24-25	0.0	0.0	0.0	0.0010336060392124292	0.0
26-27	0.0	0.0	0.0	0.002500957469880074	0.0
28-29	0.0	0.0	0.0	0.008527249823502541	0.0
30-31	0.0	0.0	0.0	0.0283411084501908	0.0
32-33	0.0	0.0	0.0	0.07233396549416982	0.0
34-35	0.0	0.0	0.0	0.1433943806900243	0.0
36-37	0.0	0.0	0.0	0.25513457642916804	0.0
38-39	0.0	0.0	0.0	0.4165893769293595	0.0
40-41	0.0	0.0	0.0	0.5976088631717862	0.0
42-43	0.0	0.0	0.0	0.784617727266435	0.0
44-45	0.0	0.0	0.0	1.0160993369232685	0.0
46-47	0.0	0.0	0.0	1.288472985506444	0.0
48-49	0.0	0.0	0.0	1.5947802895019771	0.0
50-51	0.0	0.0	0.0	1.9204492494820433	0.0
52-53	0.0	1.8457250700221948E-5	0.0	2.2441986553892868	0.0
54-55	0.0	1.8457250700221948E-5	0.0	2.591905572705418	0.0
56-57	0.0	1.8457250700221948E-5	0.0	3.00624316504935	0.0
58-59	0.0	1.8457250700221948E-5	0.0	3.4793117291213886	0.0
60-61	0.0	1.8457250700221948E-5	0.0	3.9700254248628397	0.0
62-63	0.0	1.8457250700221948E-5	0.0	4.475126547525113	0.0
64-65	0.0	1.8457250700221948E-5	0.0	5.0094270407951385	0.0
66-67	0.0	1.8457250700221948E-5	0.0	5.599200801044681	0.0
68-69	0.0	1.8457250700221948E-5	0.0	6.243128134848674	0.0
70-71	0.0	1.8457250700221948E-5	0.0	6.945851040758224	0.0
72-73	0.0	1.8457250700221948E-5	0.0	7.663819635746157	0.0
74-75	0.0	1.8457250700221948E-5	0.0	8.382341948255098	0.0
76-77	0.0	1.8457250700221948E-5	0.0	9.179547520499083	0.0
78-79	0.0	1.8457250700221948E-5	0.0	10.019324741483132	0.0
80-81	0.0	1.8457250700221948E-5	0.0	10.928482767849314	0.0
82-83	0.0	1.8457250700221948E-5	0.0	11.868833547898873	0.0
84-85	0.0	1.8457250700221948E-5	0.0	12.793975553371448	0.0
86-87	0.0	1.8457250700221948E-5	0.0	13.75303275700568	0.0
88	0.0	1.8457250700221948E-5	0.0	14.50149273015038	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14200	0.0	59.476604	1
GTATCAA	26325	0.0	43.58626	1
GTGGTAT	2820	0.0	41.522015	1
CAACGCA	32945	0.0	34.367737	5
ATCAACG	33070	0.0	34.183174	3
TATCAAC	33970	0.0	33.72111	2
AACGCAG	36790	0.0	31.554317	6
TCAACGC	39305	0.0	29.047516	4
ACGCAGA	39050	0.0	28.95731	7
CGCAGAG	39390	0.0	28.6713	8
GCAGAGT	41655	0.0	26.864073	9
TGGTATC	4585	0.0	25.742725	2
TAGGCAT	5480	0.0	25.04413	5
GTACATG	27310	0.0	24.485336	1
GTGATCG	3015	0.0	24.473267	8
TACATGG	26985	0.0	24.466106	2
GTACTAG	3815	0.0	24.1596	1
GAGTACT	27075	0.0	23.95445	12-13
ACATGGG	26745	0.0	23.823679	3
GTATAAG	3950	0.0	23.095789	1
>>END_MODULE