FastQCFastQC Report
Mon 16 Jan 2017
ERR1146577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4112405
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT217880.5298116309069754No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60650.14748061049434577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16750.027.7910331
GCATCGT18000.022.1915558
TAGGCAT27150.021.4636555
CATGGGG82550.020.7222144
TGATCGC11450.020.1109169
CTAGGCA30250.019.2640744
GTATAAG24750.019.1880321
CCGTATC5400.019.14891294
TCTAACG8100.019.1461182
CATCGTC22350.018.7134469
CGACGAG11200.018.25175324-25
GTGATCG12150.018.1787038
TAACGCC9100.017.5585784
ACGTTAA4103.8198777E-1117.192888
CTAACGC8750.017.186753
GTCTTAT11100.016.944211
GTCCTAC19500.016.879041
CTCGTCG14200.016.8780829
TACCTGG39150.016.6853332
ACTAGGC27350.016.6673373
Produced by FastQC (version 0.11.5)