##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146577_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4112405 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.747639398356924 33.0 31.0 34.0 30.0 34.0 2 31.874250712174508 33.0 31.0 34.0 30.0 34.0 3 31.92832466646646 34.0 31.0 34.0 30.0 34.0 4 35.40597776726757 37.0 35.0 37.0 33.0 37.0 5 35.40619880580828 37.0 35.0 37.0 33.0 37.0 6 35.49865006000139 37.0 35.0 37.0 33.0 37.0 7 35.394958181404796 37.0 35.0 37.0 33.0 37.0 8 35.404401074310535 37.0 35.0 37.0 33.0 37.0 9 37.04946813361038 39.0 38.0 39.0 34.0 39.0 10-11 37.026684263831015 39.0 38.0 39.0 34.0 39.0 12-13 37.02036168616661 39.0 38.0 39.0 34.0 39.0 14-15 38.46419236432209 41.0 39.0 41.0 34.0 41.0 16-17 38.484480127808425 41.0 39.0 41.0 34.0 41.0 18-19 38.52134286384731 41.0 39.0 41.0 34.0 41.0 20-21 38.58749563819711 41.0 39.0 41.0 34.0 41.0 22-23 38.60194509052489 41.0 39.0 41.0 34.0 41.0 24-25 38.65431651308663 41.0 39.0 41.0 34.0 41.0 26-27 38.66156750125535 41.0 39.0 41.0 34.0 41.0 28-29 38.61872882169922 41.0 39.0 41.0 34.0 41.0 30-31 38.612330497604205 40.5 39.0 41.0 34.0 41.0 32-33 38.59821260308749 40.0 39.0 41.0 34.0 41.0 34-35 38.53739952655441 40.0 38.0 41.0 34.0 41.0 36-37 38.46131752587598 40.0 38.0 41.0 33.0 41.0 38-39 38.420483269522336 40.0 38.0 41.0 33.0 41.0 40-41 38.36029780627151 40.0 38.0 41.0 33.0 41.0 42-43 38.25374409864787 40.0 38.0 41.0 33.0 41.0 44-45 38.139569911037455 40.0 38.0 41.0 33.0 41.0 46-47 38.02771529068757 40.0 38.0 41.0 33.0 41.0 48-49 37.90879254353596 40.0 37.5 41.0 33.0 41.0 50-51 37.77490738387878 40.0 37.0 41.0 32.0 41.0 52-53 37.672414925086414 40.0 37.0 41.0 32.0 41.0 54-55 37.48906673831979 40.0 36.5 41.0 32.0 41.0 56-57 37.324034840926416 39.5 36.0 41.0 31.5 41.0 58-59 37.09644368684505 39.0 35.5 41.0 31.0 41.0 60-61 36.88076818795814 39.0 35.0 41.0 31.0 41.0 62-63 36.631530941140284 39.0 35.0 41.0 31.0 41.0 64-65 36.385468235740404 38.0 35.0 40.0 31.0 41.0 66-67 36.087865981098645 37.0 35.0 40.0 30.5 41.0 68-69 35.7622780343862 37.0 35.0 40.0 30.0 41.0 70-71 35.440454916283784 36.0 35.0 39.0 30.0 41.0 72-73 35.102837512355904 36.0 34.5 39.0 29.5 41.0 74-75 34.78358126205955 35.0 34.0 38.5 29.0 40.0 76-77 34.44565017307391 35.0 34.0 37.0 29.0 39.5 78-79 34.14571376116895 35.0 34.0 37.0 29.0 39.0 80-81 33.85762029761174 35.0 34.0 36.0 29.0 39.0 82-83 33.60792066929206 35.0 34.0 36.0 29.0 37.0 84-85 33.39510116343113 35.0 34.0 36.0 28.5 37.0 86-87 33.232385064214256 35.0 34.0 35.0 28.0 37.0 88-89 33.08971441285574 35.0 34.0 35.0 28.0 36.0 90-91 32.91992909258694 35.0 33.5 35.0 27.5 36.0 92-93 32.80806669090228 35.0 33.0 35.0 27.0 36.0 94-95 32.72345087606887 35.0 33.0 35.0 27.0 36.0 96-97 32.66282297098656 35.0 33.0 35.0 27.0 35.0 98-99 32.62639902927849 35.0 33.0 35.0 27.0 35.0 100 31.689990650239945 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19972271698382116 1101 2 0.2515459907373163 1101 3 0.2900509464743699 1101 4 0.26072711308946594 1101 5 0.21535007843689868 1101 6 0.23346735370618177 1101 7 0.18945165836802857 1101 8 0.2730003836459147 1101 9 0.3650337931129144 1101 10-11 0.37924716013336734 1101 12-13 0.3545017466560054 1101 14-15 0.46348967981729317 1101 16-17 0.43667903448965006 1101 18-19 0.4144956466422869 1101 20-21 0.39952951626843003 1101 22-23 0.44227740286823547 1101 24-25 0.39297654511820923 1101 26-27 0.45130298154904125 1101 28-29 0.4804981459979345 1101 30-31 0.43517309138484705 1101 32-33 0.46050660599545523 1101 34-35 0.4600155735084499 1101 36-37 0.5071677439256987 1101 38-39 0.4536729663816246 1101 40-41 0.4504389030928877 1101 42-43 0.4084366604839218 1101 44-45 0.3950220719318125 1101 46-47 0.404456431272294 1101 48-49 0.38514767885182266 1101 50-51 0.382122635511827 1101 52-53 0.40754588326870334 1101 54-55 0.385239903870108 1101 56-57 0.3467413412413336 1101 58-59 0.4524804795862991 1101 60-61 0.46650202288908105 1101 62-63 0.46328746407812815 1101 64-65 0.42841079707882557 1101 66-67 0.3191861816861845 1101 68-69 0.3424919946837335 1101 70-71 0.3523380868283752 1101 72-73 0.3679586451371648 1101 74-75 0.38745850583833175 1101 76-77 0.4208666750560752 1101 78-79 0.3482971374343151 1101 80-81 0.3596113739049258 1101 82-83 0.39523470282092177 1101 84-85 0.4764872739296493 1101 86-87 0.4296465166304557 1101 88-89 0.39474714758397056 1101 90-91 0.4073776321681635 1101 92-93 0.43638764839103317 1101 94-95 0.3605991497303336 1101 96-97 0.35714732612141376 1101 98-99 0.3195360256896578 1101 100 0.22478252766733675 1102 1 0.22478065540240522 1102 2 0.22574239673272345 1102 3 0.28341014769568673 1102 4 0.2833271996115343 1102 5 0.24084795514672663 1102 6 0.21535757640947395 1102 7 0.24100112013248776 1102 8 0.2834356361662671 1102 9 0.3400518634090517 1102 10-11 0.3708469446768845 1102 12-13 0.3721672674093526 1102 14-15 0.44692654713961133 1102 16-17 0.42332200772325734 1102 18-19 0.3640458062811547 1102 20-21 0.3495515617866971 1102 22-23 0.35838860709490206 1102 24-25 0.40113920062715636 1102 26-27 0.3869048503689214 1102 28-29 0.4151995751300248 1102 30-31 0.439881631238741 1102 32-33 0.37382279817344966 1102 34-35 0.4120939856389185 1102 36-37 0.43219168966184185 1102 38-39 0.38668468776291576 1102 40-41 0.4210958378524694 1102 42-43 0.3911656522528162 1102 44-45 0.35817580996973675 1102 46-47 0.37462850670240755 1102 48-49 0.40813889433063366 1102 50-51 0.3770761316752953 1102 52-53 0.3266272411200859 1102 54-55 0.35399968653378977 1102 56-57 0.36506045095009654 1102 58-59 0.35682162404574314 1102 60-61 0.35752653773719345 1102 62-63 0.37919496270610153 1102 64-65 0.3509486446892467 1102 66-67 0.298896079639718 1102 68-69 0.29648096231602494 1102 70-71 0.34855460630744517 1102 72-73 0.27127398974134564 1102 74-75 0.19600262938326551 1102 76-77 0.2212791372998737 1102 78-79 0.22649672390652142 1102 80-81 0.2309311622335457 1102 82-83 0.2140114014427752 1102 84-85 0.2906741378964455 1102 86-87 0.31306946833860394 1102 88-89 0.2730551390086333 1102 90-91 0.31163228072918514 1102 92-93 0.2689520586731433 1102 94-95 0.3142769041106206 1102 96-97 0.2847357154144987 1102 98-99 0.21790244773887224 1102 100 0.14823326499112355 1103 1 0.20809024565880563 1103 2 0.16180549273558498 1103 3 0.22071305982491296 1103 4 0.19436200771870205 1103 5 0.21437118187960635 1103 6 0.19092620276974515 1103 7 0.16309824355563052 1103 8 0.24549229760203417 1103 9 0.25879861306635377 1103 10-11 0.24751259081922683 1103 12-13 0.2586203974231367 1103 14-15 0.31261888338369204 1103 16-17 0.31768337135525826 1103 18-19 0.2072087995463363 1103 20-21 0.24341501079518224 1103 22-23 0.310847159023524 1103 24-25 0.2986406860662285 1103 26-27 0.24638985452985196 1103 28-29 0.275755738597816 1103 30-31 0.27418934123124217 1103 32-33 0.22529171300019613 1103 34-35 0.2895871776580847 1103 36-37 0.2867035761264205 1103 38-39 0.265091105599474 1103 40-41 0.2706148138075335 1103 42-43 0.2611932192135171 1103 44-45 0.24625200401342795 1103 46-47 0.21321016615888766 1103 48-49 0.19380821115954205 1103 50-51 0.21066271105962642 1103 52-53 0.23834638618721016 1103 54-55 0.3257479265027783 1103 56-57 0.21288855515141591 1103 58-59 0.2533835465997569 1103 60-61 0.1627902594561519 1103 62-63 0.18210559813685734 1103 64-65 0.19368622584600104 1103 66-67 0.15844617067954658 1103 68-69 0.21193867281001388 1103 70-71 0.17277715776573643 1103 72-73 0.18446276452388588 1103 74-75 0.17803457843368875 1103 76-77 0.20716568781124067 1103 78-79 0.21714389945963575 1103 80-81 0.24266902955917402 1103 82-83 0.15694802752250325 1103 84-85 0.16971998985985692 1103 86-87 0.14816101285592964 1103 88-89 0.16240079394019347 1103 90-91 0.1815930002642716 1103 92-93 0.2407935898352136 1103 94-95 0.21673605355812953 1103 96-97 0.17265052600847497 1103 98-99 0.10845242332950988 1103 100 0.12635198103969358 1104 1 0.1740145114750753 1104 2 0.19282765938632096 1104 3 0.1682677014803211 1104 4 0.09461754821425927 1104 5 0.10461455158880284 1104 6 0.09914520836401408 1104 7 0.026182665889457724 1104 8 0.06542911808897145 1104 9 0.07230887286785759 1104 10-11 0.14748020141489349 1104 12-13 0.09960136053656043 1104 14-15 0.20144232801701634 1104 16-17 0.2407154691115636 1104 18-19 0.1568348558200654 1104 20-21 0.1582809137259389 1104 22-23 0.16762870264524565 1104 24-25 0.19169541138787594 1104 26-27 0.231855171856985 1104 28-29 0.22179741858916913 1104 30-31 0.2259632255074635 1104 32-33 0.23539637015836234 1104 34-35 0.300478427490269 1104 36-37 0.27584712331207584 1104 38-39 0.2589257976560404 1104 40-41 0.214236658825115 1104 42-43 0.1309869634333225 1104 44-45 0.12209699465341828 1104 46-47 0.1495725729480739 1104 48-49 0.1896729015467855 1104 50-51 0.207192012816364 1104 52-53 0.14356681254022163 1104 54-55 0.17054238933852872 1104 56-57 0.15166726219094073 1104 58-59 0.15735843312346276 1104 60-61 0.20258114121691762 1104 62-63 0.1732348762442939 1104 64-65 0.1981436788136719 1104 66-67 0.16557490750744108 1104 68-69 0.1800324084731315 1104 70-71 0.13206613080919993 1104 72-73 0.14256747183512175 1104 74-75 0.15613888425368572 1104 76-77 0.15913431534015388 1104 78-79 0.20244253456954198 1104 80-81 0.20640460130168492 1104 82-83 0.20600275244399313 1104 84-85 0.17966770196343873 1104 86-87 0.11370859720259574 1104 88-89 0.15105139726746586 1104 90-91 0.16655845434517857 1104 92-93 0.11962707241612947 1104 94-95 0.13033769262938932 1104 96-97 0.10782376513765257 1104 98-99 0.07977774653495828 1104 100 0.053407694483265544 1105 1 0.23360714577145814 1105 2 0.24959957109844666 1105 3 0.2588146764502959 1105 4 0.19088287008459304 1105 5 0.2205984530284013 1105 6 0.18343409903150842 1105 7 0.19363840408099264 1105 8 0.22963458621385513 1105 9 0.30917254152198126 1105 10-11 0.2958719591075436 1105 12-13 0.27736760023919516 1105 14-15 0.33763903251175265 1105 16-17 0.3685292988871751 1105 18-19 0.37012424790079024 1105 20-21 0.3719117551971678 1105 22-23 0.30136499514117077 1105 24-25 0.36737063979123974 1105 26-27 0.3228281469477565 1105 28-29 0.3943407542828723 1105 30-31 0.3464329240555273 1105 32-33 0.3398095065324682 1105 34-35 0.29646414441886293 1105 36-37 0.3153061525683398 1105 38-39 0.37381362770675963 1105 40-41 0.3104077380664876 1105 42-43 0.2998455406100291 1105 44-45 0.24571177766209473 1105 46-47 0.22931227325492642 1105 48-49 0.25189037755402666 1105 50-51 0.2145021115435526 1105 52-53 0.2470824664293403 1105 54-55 0.27769705028186564 1105 56-57 0.22897022597037875 1105 58-59 0.2476396093563551 1105 60-61 0.24158965554988754 1105 62-63 0.22321764461858606 1105 64-65 0.19694175668845304 1105 66-67 0.1778045474809673 1105 68-69 0.1555345984779919 1105 70-71 0.13610448526290497 1105 72-73 0.1363502772096652 1105 74-75 0.20813395684172775 1105 76-77 0.17949021642844798 1105 78-79 0.16430614858795423 1105 80-81 0.16492949875997454 1105 82-83 0.1486658442147757 1105 84-85 0.22275557128219248 1105 86-87 0.21171353570006346 1105 88-89 0.18493191535075937 1105 90-91 0.17954789483155054 1105 92-93 0.18943623481406036 1105 94-95 0.1812413972499698 1105 96-97 0.1501290769854151 1105 98-99 0.11979027505597628 1105 100 0.05349989421201329 1106 1 0.10738236637844523 1106 2 0.09250349042064343 1106 3 0.10469354625798033 1106 4 0.09184656208942954 1106 5 0.08368357859797726 1106 6 0.09789770742447956 1106 7 0.09505512264620819 1106 8 0.09083348188072193 1106 9 0.10495907250556513 1106 10-11 0.14250224150868718 1106 12-13 0.12862650043226154 1106 14-15 0.16821181958673748 1106 16-17 0.1981651766399679 1106 18-19 0.15271556357764382 1106 20-21 0.18401252573622884 1106 22-23 0.1546476807449011 1106 24-25 0.12896648313890324 1106 26-27 0.14939206631935065 1106 28-29 0.1299359180437918 1106 30-31 0.17262991410967032 1106 32-33 0.14247199968165347 1106 34-35 0.15725779843305787 1106 36-37 0.1909271961475767 1106 38-39 0.1362887633763492 1106 40-41 0.1722833130446091 1106 42-43 0.1722371872766999 1106 44-45 0.12289859370445555 1106 46-47 0.10527346972148877 1106 48-49 0.13220170443415213 1106 50-51 0.110531921947981 1106 52-53 0.1078621616857447 1106 54-55 0.08247148941725868 1106 56-57 0.0779729344695923 1106 58-59 0.12029454977027854 1106 60-61 0.10804568826083738 1106 62-63 0.06801362432126723 1106 64-65 0.08887528911671438 1106 66-67 0.10901067762967642 1106 68-69 0.045116775810981835 1106 70-71 0.06528337548194685 1106 72-73 0.05116166656812737 1106 74-75 0.06549075522728032 1106 76-77 0.058215450145411296 1106 78-79 0.05381176377574093 1106 80-81 0.0707631403166431 1106 82-83 0.05533397876543944 1106 84-85 0.12490477292720925 1106 86-87 0.045150072137403185 1106 88-89 0.008531794889648836 1106 90-91 0.024133883546312518 1106 92-93 0.01899802016825447 1106 94-95 0.04409697706565652 1106 96-97 0.04526823110720812 1106 98-99 0.09263337889972689 1106 100 0.01782130928804193 1107 1 0.0735326624403001 1107 2 0.10123237458127576 1107 3 0.13693113068971385 1107 4 0.11544085092906187 1107 5 0.13907904746309185 1107 6 0.07981649581348194 1107 7 0.08900802346038006 1107 8 0.09634897669895537 1107 9 0.13779857778300197 1107 10-11 0.19545500142455552 1107 12-13 0.13934649075246597 1107 14-15 0.15136453686458395 1107 16-17 0.16644971785561324 1107 18-19 0.11715245197353852 1107 20-21 0.132162982715208 1107 22-23 0.14526357083609298 1107 24-25 0.13303586135638312 1107 26-27 0.16703880464750398 1107 28-29 0.1575397939824157 1107 30-31 0.10880837892317174 1107 32-33 0.15525687686854894 1107 34-35 0.14271288653193892 1107 36-37 0.17307438778852458 1107 38-39 0.16999799651367908 1107 40-41 0.13448830925296562 1107 42-43 0.07739140281147883 1107 44-45 0.12811218465024155 1107 46-47 0.1452317887077541 1107 48-49 0.13280502571285524 1107 50-51 0.09780631993058364 1107 52-53 0.09899010904809558 1107 54-55 0.17424473861729695 1107 56-57 0.17893629675415212 1107 58-59 0.15322198749884564 1107 60-61 0.1517019758910223 1107 62-63 0.16789400965321022 1107 64-65 0.09846964087264354 1107 66-67 0.09732419689982663 1107 68-69 0.11281683230887296 1107 70-71 0.14573463980907064 1107 72-73 0.14056899477162688 1107 74-75 0.114544296906125 1107 76-77 0.10035100020792953 1107 78-79 0.10242837035793428 1107 80-81 0.1439331996723041 1107 82-83 0.12375475705180605 1107 84-85 0.14140681206085048 1107 86-87 0.14656005461842625 1107 88-89 0.06878462876901636 1107 90-91 0.08757113991703136 1107 92-93 0.10564388612983322 1107 94-95 0.09125257823307464 1107 96-97 0.04446896713941584 1107 98-99 0.03965233023721737 1107 100 0.037609162262388196 1108 1 0.10565274915301259 1108 2 0.13268881322501969 1108 3 0.12057570520542882 1108 4 0.14191579263028586 1108 5 0.056015491785323945 1108 6 0.07878743593690984 1108 7 0.04776414081969449 1108 8 -0.005347851126145997 1108 9 0.03715968208279463 1108 10-11 0.10968543770491124 1108 12-13 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-0.3071253963209415 2214 76-77 -0.3102427030364936 2214 78-79 -0.29432979075819077 2214 80-81 -0.2659550647639719 2214 82-83 -0.2682099258626778 2214 84-85 -0.2395358886875414 2214 86-87 -0.22825132559881212 2214 88-89 -0.261328324377736 2214 90-91 -0.2549011125277545 2214 92-93 -0.2646686517213226 2214 94-95 -0.2168081277782008 2214 96-97 -0.24982269988559125 2214 98-99 -0.22469816263553355 2214 100 -0.1794765256246471 2215 1 -0.25964317519934355 2215 2 -0.36346277987779985 2215 3 -0.32220705211561196 2215 4 -0.26693729905595376 2215 5 -0.28732127779755245 2215 6 -0.24863225781319187 2215 7 -0.233857263318896 2215 8 -0.19960920785711522 2215 9 -0.3427256948601709 2215 10-11 -0.33339985586003706 2215 12-13 -0.4109999101916415 2215 14-15 -0.5026175505717916 2215 16-17 -0.5223833189402285 2215 18-19 -0.5431888718844178 2215 20-21 -0.5569865103971665 2215 22-23 -0.42016039747490197 2215 24-25 -0.40943204515466647 2215 26-27 -0.42402419580102446 2215 28-29 -0.40904027745427385 2215 30-31 -0.45580109931184865 2215 32-33 -0.4756933623965409 2215 34-35 -0.47862467726388047 2215 36-37 -0.4729513953441682 2215 38-39 -0.48476201046485556 2215 40-41 -0.4651223842409138 2215 42-43 -0.48971425148417325 2215 44-45 -0.4556914172922859 2215 46-47 -0.39547808687718344 2215 48-49 -0.395461889102279 2215 50-51 -0.4470614567223947 2215 52-53 -0.43315046842108273 2215 54-55 -0.35309496034967225 2215 56-57 -0.40047571103202273 2215 58-59 -0.4148199146182989 2215 60-61 -0.39154098353821354 2215 62-63 -0.4124348751645073 2215 64-65 -0.407713129549542 2215 66-67 -0.3512636293027498 2215 68-69 -0.32021807282178827 2215 70-71 -0.39391458490509734 2215 72-73 -0.29154186676754534 2215 74-75 -0.27525645944839283 2215 76-77 -0.3034728906873525 2215 78-79 -0.3016111812961739 2215 80-81 -0.3184629961321974 2215 82-83 -0.30449333922004485 2215 84-85 -0.26162125343147125 2215 86-87 -0.3311673204622636 2215 88-89 -0.31983895688139796 2215 90-91 -0.30777506890004247 2215 92-93 -0.3729340858420471 2215 94-95 -0.4108309620610271 2215 96-97 -0.3706777406683628 2215 98-99 -0.4626165288656239 2215 100 -0.359576175537736 2216 1 -0.16397812901250575 2216 2 -0.19167658025214607 2216 3 -0.189397821859405 2216 4 -0.09543174636088025 2216 5 -0.07508969332545234 2216 6 -0.08642352371364836 2216 7 -0.08629890106781346 2216 8 -0.0575810793211744 2216 9 -0.09046931782473422 2216 10-11 -0.1444797306552701 2216 12-13 -0.19047673446031865 2216 14-15 -0.36173263871823735 2216 16-17 -0.2968513270007165 2216 18-19 -0.3593443143341659 2216 20-21 -0.2909991427084577 2216 22-23 -0.3485643885715035 2216 24-25 -0.39466781046326815 2216 26-27 -0.3931387305939751 2216 28-29 -0.3782055593435274 2216 30-31 -0.4325556352653166 2216 32-33 -0.40637219624731813 2216 34-35 -0.46827308401665846 2216 36-37 -0.4336376438408962 2216 38-39 -0.4383901369938954 2216 40-41 -0.4888339813448894 2216 42-43 -0.4210223013377572 2216 44-45 -0.40976081596429026 2216 46-47 -0.3810579275331918 2216 48-49 -0.35613724504273137 2216 50-51 -0.42370901584814646 2216 52-53 -0.33626054773677794 2216 54-55 -0.388577993229994 2216 56-57 -0.4358110254338712 2216 58-59 -0.4644964500524438 2216 60-61 -0.5585435549231832 2216 62-63 -0.47618273182864357 2216 64-65 -0.40212395387100486 2216 66-67 -0.4821129338406607 2216 68-69 -0.4257573521577598 2216 70-71 -0.4871537250155811 2216 72-73 -0.41324114044927285 2216 74-75 -0.3689370607582205 2216 76-77 -0.3696135031180603 2216 78-79 -0.3766306668286745 2216 80-81 -0.36279107556438106 2216 82-83 -0.37999361695115397 2216 84-85 -0.3973296124358683 2216 86-87 -0.36418548710834386 2216 88-89 -0.38390026903013563 2216 90-91 -0.34502168517251164 2216 92-93 -0.35115505945188374 2216 94-95 -0.3760603081782605 2216 96-97 -0.3043837598818797 2216 98-99 -0.28526994830298946 2216 100 -0.2894078986317936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 138.0 1 74.0 2 48.0 3 47.0 4 42.0 5 64.0 6 164.0 7 1017.0 8 2646.0 9 3070.0 10 2799.0 11 3118.0 12 3823.0 13 4784.0 14 6164.0 15 7277.0 16 7671.0 17 8237.0 18 9334.0 19 10364.0 20 11549.0 21 13256.0 22 15360.0 23 18645.0 24 23327.0 25 30076.0 26 34314.0 27 32535.0 28 34134.0 29 43626.0 30 50804.0 31 57270.0 32 73146.0 33 100670.0 34 152293.0 35 242888.0 36 451603.0 37 985351.0 38 1259727.0 39 410192.0 40 758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.263981500338325 19.729225411827453 12.95881445665638 24.047978631177838 2 14.816835109526364 21.590922908634237 39.23958250749396 24.352659474345437 3 18.937565541540934 26.752160074087477 30.008735629500354 24.30153875487123 4 12.227943992850152 17.756066123942873 39.5595303959776 30.45645948722937 5 12.85558569587911 38.90416982215912 35.305741075002715 12.934503406959058 6 29.518556196330564 37.881265928006805 18.886800891372545 13.713376984290083 7 25.96103223741821 32.99660941606232 23.455360796990277 17.5869975495292 8 25.224432365340583 34.17091586108685 21.490791196434373 19.1138605771382 9 25.993818719566896 16.4483147633091 21.222866311632544 36.33500020549146 10-11 24.166354681318367 26.350223747907247 28.746514407278706 20.736907163495683 12-13 25.682636465585386 24.34122588397998 27.83998741283859 22.13615023759605 14-15 23.108999379856275 25.583713323362396 26.09897980276997 25.208307494011358 16-17 22.027512046810532 28.416392052442855 27.21253177149065 22.343564129255963 18-19 21.846619269440705 28.872765563690173 27.777473793516865 21.503141373352253 20-21 23.196409227363517 27.716391800725443 26.747700776891005 22.33949819502003 22-23 22.794137226124015 27.612698583306045 26.228103569170962 23.365060621398975 24-25 23.105373029544058 27.75944688682947 26.33793786778389 22.797242215842584 26-27 23.116729028451104 27.192712930344037 26.962517635194445 22.728040406010415 28-29 22.475403764439353 28.252227018080482 26.830853140465745 22.44151607701442 30-31 22.957811198361092 27.45279764474909 26.78271066646709 22.806680490422735 32-33 22.721830159998436 27.92304082750104 26.698851748192737 22.656277264307793 34-35 23.00896265481455 27.86604134273354 26.967477651122213 22.157518351329696 36-37 23.212916564771252 27.07440722405759 27.09796224168372 22.614713969487436 38-39 23.102229360785888 27.624490256533484 26.936392819679412 22.336887563001216 40-41 22.835074739397125 27.448049673851184 27.1984035489661 22.518472037785592 42-43 22.840024230221644 27.386912153528442 26.52375003177146 23.249313584478454 44-45 23.038358285519557 27.3264079675759 26.746160486449057 22.889073260455486 46-47 23.148217629177115 27.544547868701528 26.88388395753761 22.423350544583748 48-49 23.20734699943726 27.3314051485477 26.960642481775952 22.50060537023909 50-51 22.20435456290181 27.42037999654593 27.3758419299991 22.999423510553164 52-53 22.55238455233858 27.96132088769604 26.21748195369214 23.268812606273244 54-55 22.59935031384794 27.331954400341722 26.595827491152807 23.47286779465753 56-57 23.277274660091486 26.764760830706923 26.72021975220467 23.237744756996914 58-59 23.323801395625527 26.988945165877244 26.907929168592943 22.77932426990429 60-61 23.022548672609425 27.486088761908935 27.19289155498147 22.298471010500172 62-63 22.50718487788444 27.449067334133616 26.912916725957874 23.130831062024075 64-65 21.993042762043657 27.934656792112715 26.748367569166454 23.323932876677176 66-67 23.188484767031085 26.95971261784973 26.326408860341495 23.525393754777692 68-69 22.888877050149386 26.926304819648156 26.682021349626034 23.502796780576425 70-71 22.998603192952036 27.010129891865436 26.643230861894658 23.348036053287874 72-73 23.280041605883984 26.52466323534885 27.39960549331944 22.79568966544773 74-75 22.88204488996442 27.71713746931293 26.635694984883912 22.76512265583874 76-77 22.772574075222796 27.018175051175486 26.765709323242977 23.443541550358745 78-79 22.95964511717448 27.2415406149246 26.780045527986967 23.018768739913956 80-81 23.057152911042778 26.98822256693233 26.993793393607735 22.960831128417166 82-83 23.096465191893007 26.521210782550693 27.49582780476668 22.886496220789613 84-85 22.98306785022413 27.299503575289297 26.659464032560482 23.057964541926093 86-87 22.654161022833456 27.457787249268765 26.87268132561252 23.015370402285253 88-89 22.856157378685683 26.99184664259461 26.550970129004725 23.601025849714986 90-91 23.264282053421375 26.556615327807904 26.62490686688149 23.554195751889235 92-93 23.541590752194242 27.033368637110417 26.700346340269704 22.724694270425637 94-95 22.73834654798214 26.90576529090634 27.19971966813611 23.156168492975404 96-97 22.581349012812147 26.729162901381432 27.62036486799751 23.069123217808908 98-99 22.93219667799041 27.230982460766224 26.63479543074087 23.202025430502495 100 22.940777991961035 26.377758453988775 26.3590535667593 24.322409987290893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1927.0 1 1341.0 2 769.0 3 754.0 4 685.5 5 557.5 6 445.5 7 453.0 8 529.0 9 556.5 10 459.5 11 377.5 12 387.5 13 408.5 14 420.5 15 495.0 16 609.5 17 713.5 18 913.0 19 1223.0 20 1669.0 21 2411.5 22 3655.0 23 5533.5 24 7679.5 25 10549.0 26 15064.0 27 20656.5 28 26457.0 29 33937.0 30 42886.5 31 50624.5 32 60745.5 33 72174.5 34 81212.5 35 87981.0 36 95585.5 37 107234.0 38 113602.0 39 115348.0 40 116230.5 41 116307.5 42 118508.0 43 127510.5 44 143861.5 45 158599.5 46 163775.5 47 178526.0 48 216058.5 49 220889.5 50 204483.0 51 188374.5 52 160783.5 53 146293.5 54 136288.5 55 121899.0 56 109863.5 57 96420.0 58 82209.5 59 74783.0 60 64292.0 61 50049.0 62 38645.5 63 30083.0 64 22462.0 65 15053.5 66 10404.5 67 7674.5 68 5586.0 69 4146.5 70 3214.0 71 2467.0 72 1850.5 73 1689.5 74 1425.5 75 1021.5 76 795.5 77 592.0 78 367.5 79 210.5 80 137.0 81 102.5 82 69.5 83 46.0 84 41.5 85 38.0 86 25.0 87 24.0 88 19.0 89 12.0 90 13.5 91 16.0 92 12.0 93 11.5 94 10.5 95 5.0 96 8.0 97 10.5 98 10.0 99 10.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06059714449330744 2 0.010383218578909422 3 0.012377185612798351 4 0.010456168592344382 5 0.012814885693408114 6 0.013228269102872893 7 0.0032584339334282494 8 0.0061521177996816945 9 0.007586801397235924 10-11 0.007282843007923587 12-13 0.006772192913878861 14-15 0.011064085370969055 16-17 0.010322426901046953 18-19 0.008510834900745426 20-21 0.007526009719373457 22-23 0.002905842201825939 24-25 0.004632325853120012 26-27 0.0059454260949493055 28-29 0.007100467974336186 30-31 0.009763143464712255 32-33 0.01114919371997651 34-35 0.012389343948370843 36-37 0.01834692837889264 38-39 0.018918370150799835 40-41 0.019088586848814745 42-43 0.0245719961920093 44-45 0.02776963844757508 46-47 0.030091880541921332 48-49 0.03123476408573572 50-51 0.031976422555657816 52-53 0.03028641391108123 54-55 0.03640205670404544 56-57 0.03873645713396419 58-59 0.03619536499931306 60-61 0.03696134014038015 62-63 0.032584339334282494 64-65 0.03488226475748376 66-67 0.03653579839534287 68-69 0.039076890529994 70-71 0.03893099050312408 72-73 0.03521053981794108 74-75 0.03776379028816471 76-77 0.03769084027472975 78-79 0.03713155683839505 80-81 0.04144776596663023 82-83 0.03792184865060713 84-85 0.03426218964328659 86-87 0.0329369310658848 88-89 0.031040230716575825 90-91 0.032924772730312316 92-93 0.034505356354736456 94-95 0.03800695699961458 96-97 0.03365427286466192 98-99 0.03110102239443829 100 0.028693671951084583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4112405.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.89658568797995 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43798525030985 41.93912020109191 2 14.492348314646481 16.781149720379634 3 5.67393791773812 9.855048985278108 4 2.716721806692361 6.291556674862719 5 1.4951039098229768 4.328070581374992 6 0.8846016249697565 3.072924826787269 7 0.5801150161422625 2.3510675118675053 8 0.40061468803589734 1.8555378090986947 9 0.2810422931379271 1.4644250285945737 >10 1.012980338189252 8.997043107723622 >50 0.017310050343941893 0.6537814239059335 >100 0.006551714036220125 0.6149524715645988 >500 1.374151869844101E-4 0.05919167390013757 >1k 4.1224556091950024E-4 0.6281557274896831 >5k 6.870759348658337E-5 0.24126176222056409 >10k+ 6.870759348658337E-5 0.8667124938601238 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21788 0.5298116309069754 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6065 0.14748061049434577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4316671144986936E-5 0.0 2 0.0 0.0 0.0 2.4316671144986936E-5 0.0 3 0.0 0.0 0.0 2.4316671144986936E-5 0.0 4 0.0 0.0 0.0 2.4316671144986936E-5 0.0 5 0.0 0.0 0.0 2.4316671144986936E-5 0.0 6 0.0 0.0 0.0 2.4316671144986936E-5 0.0 7 0.0 0.0 0.0 4.863334228997387E-5 0.0 8 0.0 0.0 0.0 4.863334228997387E-5 0.0 9 0.0 0.0 0.0 4.863334228997387E-5 0.0 10-11 0.0 0.0 0.0 4.863334228997387E-5 0.0 12-13 0.0 0.0 0.0 7.295001343496081E-5 0.0 14-15 0.0 0.0 0.0 9.726668457994774E-5 0.0 16-17 0.0 0.0 0.0 1.3374169129742815E-4 0.0 18-19 0.0 0.0 0.0 2.3100837587737588E-4 0.0 20-21 0.0 0.0 0.0 2.9180005373984324E-4 0.0 22-23 0.0 0.0 0.0 4.6201675175475175E-4 0.0 24-25 0.0 0.0 0.0 9.969835169444643E-4 0.0 26-27 0.0 0.0 0.0 0.002261450416483785 0.0 28-29 0.0 0.0 0.0 0.0066749262292989135 0.0 30-31 0.0 0.0 0.0 0.017763328271412956 0.0 32-33 0.0 0.0 0.0 0.0437456913898315 0.0 34-35 0.0 0.0 0.0 0.08294416527555043 0.0 36-37 0.0 0.0 0.0 0.1403193508421471 0.0 38-39 0.0 0.0 0.0 0.22319056610426258 0.0 40-41 0.0 0.0 0.0 0.31316224934071424 0.0 42-43 0.0 0.0 0.0 0.39399086422665086 0.0 44-45 0.0 0.0 0.0 0.4927408657464428 0.0 46-47 0.0 0.0 0.0 0.6103362874035996 0.0 48-49 0.0 0.0 0.0 0.7310442429673147 0.0 50-51 0.0 0.0 0.0 0.8536488988803388 0.0 52-53 0.0 0.0 0.0 0.9750498795716862 0.0 54-55 0.0 0.0 0.0 1.1036729115930946 0.0 56-57 0.0 0.0 0.0 1.25641808139033 0.0 58-59 0.0 0.0 0.0 1.4166406275646488 0.0 60-61 0.0 0.0 0.0 1.5781276406385072 0.0 62-63 0.0 0.0 0.0 1.7392985369874805 0.0 64-65 0.0 0.0 0.0 1.9149135359965763 0.0 66-67 0.0 0.0 0.0 2.1121825306602826 0.0 68-69 0.0 0.0 0.0 2.323628144601517 0.0 70-71 0.0 0.0 0.0 2.5499555612834826 0.0 72-73 0.0 0.0 0.0 2.7615713919227316 0.0 74-75 0.0 0.0 0.0 2.97854904854945 0.0 76-77 0.0 0.0 0.0 3.2139465835684957 0.0 78-79 0.0 2.4316671144986936E-5 0.0 3.4807126243645747 0.0 80-81 0.0 2.4316671144986936E-5 0.0 3.756560941833307 0.0 82-83 0.0 2.4316671144986936E-5 0.0 4.035643376564321 0.0 84-85 0.0 2.4316671144986936E-5 0.0 4.308038726730466 0.0 86-87 0.0 2.4316671144986936E-5 0.0 4.59677487990604 0.0 88 0.0 2.4316671144986936E-5 0.0 4.8314550731263095 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1675 0.0 27.791033 1 GCATCGT 1800 0.0 22.191555 8 TAGGCAT 2715 0.0 21.463655 5 CATGGGG 8255 0.0 20.722214 4 TGATCGC 1145 0.0 20.110916 9 CTAGGCA 3025 0.0 19.264074 4 GTATAAG 2475 0.0 19.188032 1 CCGTATC 540 0.0 19.148912 94 TCTAACG 810 0.0 19.146118 2 CATCGTC 2235 0.0 18.713446 9 CGACGAG 1120 0.0 18.251753 24-25 GTGATCG 1215 0.0 18.178703 8 TAACGCC 910 0.0 17.558578 4 ACGTTAA 410 3.8198777E-11 17.19288 8 CTAACGC 875 0.0 17.18675 3 GTCTTAT 1110 0.0 16.94421 1 GTCCTAC 1950 0.0 16.87904 1 CTCGTCG 1420 0.0 16.878082 9 TACCTGG 3915 0.0 16.685333 2 ACTAGGC 2735 0.0 16.667337 3 >>END_MODULE