FastQCFastQC Report
Mon 16 Jan 2017
ERR1146577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4112405
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT233810.5685480880409395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132180.3214177591944373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130590.3175514084823844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109740.2668511491450866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60430.14694564372915606No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG58080.14123122601008412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17300.026.9292831
GAGTACT132950.022.26984812-13
GTACTTT143550.020.49444614-15
CTAGTAC26400.019.7678033
AGTACTT140900.019.67913612-13
TCTAACG7300.019.3373572
CATGGGG78400.019.0699884
CTGTGCG24900.018.3078569
TAGTACT27050.018.2499374
CGACGAG10200.017.9694524-25
GCATCGT21350.017.6099118
TAGGCAT26350.017.4857735
TGATCGC10000.017.3886879
ACTTTTT170350.017.35306416-17
GTATAAG24050.017.2188171
ATAACGC4154.5474735E-1116.9934863
GTGATCG10450.016.639898
TACTTTT165600.016.60200714-15
GTATCGG9650.016.5801071
GTACCCG5150.016.4475421
Produced by FastQC (version 0.11.5)