##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4112405
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53461076912415	34.0	31.0	34.0	31.0	34.0
2	32.5999341018212	34.0	31.0	34.0	31.0	34.0
3	32.72931678664917	34.0	31.0	34.0	31.0	34.0
4	36.16956136372755	37.0	37.0	37.0	35.0	37.0
5	36.192904395359896	37.0	37.0	37.0	35.0	37.0
6	36.2806005245106	37.0	37.0	37.0	35.0	37.0
7	36.265229713513136	37.0	37.0	37.0	35.0	37.0
8	36.282434731015066	37.0	37.0	37.0	35.0	37.0
9	38.103940638142404	39.0	39.0	39.0	37.0	39.0
10-11	38.06878566191803	39.0	38.0	39.0	36.0	39.0
12-13	38.05014948673586	39.0	38.0	39.0	35.0	39.0
14-15	39.49505569125609	41.0	39.0	41.0	37.0	41.0
16-17	39.493106831647175	41.0	39.0	41.0	37.0	41.0
18-19	39.541820054201864	41.0	39.5	41.0	37.0	41.0
20-21	39.573355615509655	41.0	40.0	41.0	37.0	41.0
22-23	39.53466146938349	41.0	39.0	41.0	37.0	41.0
24-25	39.51830972387204	41.0	39.0	41.0	37.0	41.0
26-27	39.44015509172856	41.0	39.0	41.0	36.0	41.0
28-29	39.36119484827005	41.0	39.0	41.0	36.0	41.0
30-31	39.284171792418306	41.0	39.0	41.0	36.0	41.0
32-33	39.16813835213215	41.0	39.0	41.0	35.0	41.0
34-35	39.09680661316189	41.0	39.0	41.0	35.0	41.0
36-37	39.02481333915313	41.0	39.0	41.0	35.0	41.0
38-39	38.930196442227846	40.5	39.0	41.0	35.0	41.0
40-41	38.8625455177688	40.0	39.0	41.0	35.0	41.0
42-43	38.77808630229756	40.0	38.0	41.0	35.0	41.0
44-45	38.64668351001421	40.0	38.0	41.0	35.0	41.0
46-47	38.43818398236555	40.0	38.0	41.0	34.5	41.0
48-49	38.22912042952968	40.0	38.0	41.0	34.0	41.0
50-51	38.07076467419916	40.0	38.0	41.0	34.0	41.0
52-53	37.96193711465675	40.0	37.5	41.0	34.0	41.0
54-55	37.82942171308517	40.0	37.0	41.0	33.5	41.0
56-57	37.61178920850451	40.0	37.0	41.0	33.0	41.0
58-59	37.40666203839359	40.0	36.0	41.0	33.0	41.0
60-61	37.19447257748203	39.0	35.5	41.0	33.0	41.0
62-63	36.93479849382539	39.0	35.0	41.0	32.5	41.0
64-65	36.64965901947887	38.5	35.0	41.0	32.0	41.0
66-67	36.36618377810551	37.5	35.0	40.0	32.0	41.0
68-69	36.03130601193219	37.0	35.0	40.0	31.5	41.0
70-71	35.713945610901646	36.5	35.0	39.0	31.0	41.0
72-73	35.37256714744778	36.0	35.0	39.0	31.0	41.0
74-75	35.04420175055716	35.5	35.0	38.5	31.0	40.5
76-77	34.72122772440944	35.0	35.0	37.0	31.0	39.5
78-79	34.427542155989016	35.0	35.0	37.0	31.0	39.0
80-81	34.169038190547866	35.0	34.0	36.5	30.5	39.0
82-83	33.92226446568371	35.0	34.0	36.0	30.0	37.0
84-85	33.720003744767354	35.0	34.0	36.0	30.0	37.0
86-87	33.5560350938198	35.0	34.0	35.0	30.0	37.0
88-89	33.40824420260164	35.0	34.0	35.0	30.0	36.0
90-91	33.31606748362576	35.0	34.0	35.0	30.0	36.0
92-93	33.216423601274684	35.0	34.0	35.0	30.0	36.0
94-95	33.13913549370746	35.0	34.0	35.0	29.5	36.0
96-97	33.097698427076125	35.0	34.0	35.0	29.0	35.5
98-99	33.06315221871387	35.0	34.0	35.0	29.0	35.0
100	32.486770393480214	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2660968970070883
1101	2	0.23036486017920765
1101	3	0.2618312252341113
1101	4	0.1781736129067184
1101	5	0.16561596795196465
1101	6	0.17540025218811905
1101	7	0.19691002487271447
1101	8	0.18893197404071316
1101	9	0.22292384785060193
1101	10-11	0.24548282019962642
1101	12-13	0.260870922478432
1101	14-15	0.41663408179120864
1101	16-17	0.36899907601800663
1101	18-19	0.3506798373801132
1101	20-21	0.3247760087665341
1101	22-23	0.34721474414378406
1101	24-25	0.35485098276988936
1101	26-27	0.3285942314972701
1101	28-29	0.3720150122044714
1101	30-31	0.417756149324255
1101	32-33	0.40732550603463125
1101	34-35	0.4436065173286181
1101	36-37	0.3960254727064054
1101	38-39	0.4005610678431779
1101	40-41	0.4099128831888734
1101	42-43	0.4571346315032301
1101	44-45	0.4567118133440644
1101	46-47	0.457922986307743
1101	48-49	0.4385326264074081
1101	50-51	0.4944658278467884
1101	52-53	0.45163646267273805
1101	54-55	0.4222328190104818
1101	56-57	0.43131961026986687
1101	58-59	0.43678595782368745
1101	60-61	0.4242702456042764
1101	62-63	0.3969523196866902
1101	64-65	0.444154266457069
1101	66-67	0.4478878048753785
1101	68-69	0.4307717105143709
1101	70-71	0.38961942651523884
1101	72-73	0.4214527086941331
1101	74-75	0.37823370225491004
1101	76-77	0.3703822097655092
1101	78-79	0.3755653348722774
1101	80-81	0.412804713098339
1101	82-83	0.38899490097364975
1101	84-85	0.317950561033868
1101	86-87	0.3703873840938243
1101	88-89	0.3742672267276532
1101	90-91	0.34751254393838593
1101	92-93	0.31079594945902755
1101	94-95	0.3326211436637365
1101	96-97	0.25607299872822864
1101	98-99	0.2842483892736567
1101	100	0.2551709673687128
1102	1	0.23704575308714482
1102	2	0.2557470600747962
1102	3	0.2493387648836105
1102	4	0.19157746747202253
1102	5	0.17479530077491034
1102	6	0.1592953402716759
1102	7	0.19165768435203034
1102	8	0.18008060405372817
1102	9	0.22517195607295548
1102	10-11	0.1897281473180641
1102	12-13	0.22758351715237524
1102	14-15	0.27858956281776415
1102	16-17	0.2680251811792971
1102	18-19	0.2608867758728408
1102	20-21	0.2839555902161166
1102	22-23	0.28274153893554654
1102	24-25	0.2594376694004623
1102	26-27	0.2889915226558415
1102	28-29	0.3497785000780169
1102	30-31	0.3264668601934204
1102	32-33	0.3018677886956027
1102	34-35	0.30363170227225567
1102	36-37	0.29961939130634363
1102	38-39	0.33404501159104427
1102	40-41	0.3481718977831818
1102	42-43	0.329023774830425
1102	44-45	0.31405343826980925
1102	46-47	0.3923655820251639
1102	48-49	0.43205081782024024
1102	50-51	0.456632910704478
1102	52-53	0.41274108726651804
1102	54-55	0.4041527086666221
1102	56-57	0.33897212504604823
1102	58-59	0.33156060357465833
1102	60-61	0.31869969289827793
1102	62-63	0.32127029663006823
1102	64-65	0.31367029657535994
1102	66-67	0.3183199213352381
1102	68-69	0.3146247676106739
1102	70-71	0.2750019299970319
1102	72-73	0.2740600151094128
1102	74-75	0.27738701826313417
1102	76-77	0.2560267993713481
1102	78-79	0.19252236691778535
1102	80-81	0.18570903076346923
1102	82-83	0.21228050908430873
1102	84-85	0.21295814938054036
1102	86-87	0.17349432349507055
1102	88-89	0.2067371401939866
1102	90-91	0.20413337594181513
1102	92-93	0.18459095616129417
1102	94-95	0.18863951406904533
1102	96-97	0.25661889309301245
1102	98-99	0.2292698240676927
1102	100	0.20193764899160982
1103	1	0.20470024177351576
1103	2	0.20108717031560985
1103	3	0.21148637011682325
1103	4	0.16468940203687765
1103	5	0.15029112230398312
1103	6	0.1131357257624046
1103	7	0.12640122391023567
1103	8	0.13811030828259163
1103	9	0.16476685107074474
1103	10-11	0.18041069385536446
1103	12-13	0.15178959771797906
1103	14-15	0.23547722350499356
1103	16-17	0.20599847037779995
1103	18-19	0.2160810217944018
1103	20-21	0.22510974276254814
1103	22-23	0.22807057018030008
1103	24-25	0.24616424966542638
1103	26-27	0.23923345734233692
1103	28-29	0.26242965012760067
1103	30-31	0.2788098562370678
1103	32-33	0.2846535540600357
1103	34-35	0.30061883776172493
1103	36-37	0.30741955580898406
1103	38-39	0.3385289996142049
1103	40-41	0.34621002631703135
1103	42-43	0.29753109724656923
1103	44-45	0.2721195804808687
1103	46-47	0.30730540724946565
1103	48-49	0.3076239824787308
1103	50-51	0.2927680504132866
1103	52-53	0.2695185306155068
1103	54-55	0.30790726483706266
1103	56-57	0.3416683934623421
1103	58-59	0.2790553029063574
1103	60-61	0.2692911761343595
1103	62-63	0.2551483407487325
1103	64-65	0.24257966882272797
1103	66-67	0.15999425675374113
1103	68-69	0.18765058120910538
1103	70-71	0.18823863077373204
1103	72-73	0.11516491147082775
1103	74-75	0.09989961648653178
1103	76-77	0.09277311554291146
1103	78-79	0.1325057025427938
1103	80-81	0.16596625979950375
1103	82-83	0.18465013313040401
1103	84-85	0.15443887445263726
1103	86-87	0.10638770627925709
1103	88-89	0.1343731925773355
1103	90-91	0.20729624258989787
1103	92-93	0.12978527890670932
1103	94-95	0.12200922255025404
1103	96-97	0.10745205493605425
1103	98-99	0.08253197132437862
1103	100	0.1494705824188287
1104	1	0.22143599005212167
1104	2	0.20444068914277835
1104	3	0.20245181712828497
1104	4	0.1309013143082396
1104	5	0.14095691813929534
1104	6	0.10141591675218109
1104	7	0.11297227608601901
1104	8	0.13227581377038433
1104	9	0.17860976169272647
1104	10-11	0.14995801783297225
1104	12-13	0.14030044608427517
1104	14-15	0.26955375800346104
1104	16-17	0.26496450975555064
1104	18-19	0.24228931197465187
1104	20-21	0.19834123226631561
1104	22-23	0.22118261137570272
1104	24-25	0.19984410016129317
1104	26-27	0.22553990545817015
1104	28-29	0.2969181966566623
1104	30-31	0.24868847716938802
1104	32-33	0.21148087768550283
1104	34-35	0.2191801606446404
1104	36-37	0.26090804771656195
1104	38-39	0.2786945353805734
1104	40-41	0.2268595064025689
1104	42-43	0.22917893328555294
1104	44-45	0.20415350168761393
1104	46-47	0.18407422786765437
1104	48-49	0.20247126275248206
1104	50-51	0.21055558565797838
1104	52-53	0.14577851779313278
1104	54-55	0.13596679638215647
1104	56-57	0.18693614242575052
1104	58-59	0.23815258389706173
1104	60-61	0.23027986762043184
1104	62-63	0.21940443386161235
1104	64-65	0.23194335258076393
1104	66-67	0.24368125139250907
1104	68-69	0.2719607709297165
1104	70-71	0.23173560334981147
1104	72-73	0.1949367163959863
1104	74-75	0.15410356642657774
1104	76-77	0.12643708779440033
1104	78-79	0.1416819946059178
1104	80-81	0.176248970313452
1104	82-83	0.19308358305848117
1104	84-85	0.15346557501974445
1104	86-87	0.1897074898394706
1104	88-89	0.13272391063639333
1104	90-91	0.1691848546514052
1104	92-93	0.14818424794126628
1104	94-95	0.07226025794076207
1104	96-97	0.13003345211351558
1104	98-99	0.13660371927378634
1104	100	0.1515055160237182
1105	1	0.19904824258841813
1105	2	0.2143827401404721
1105	3	0.21879587395626032
1105	4	0.14108971689995542
1105	5	0.09390110316004296
1105	6	0.13148639427551245
1105	7	0.12837701056584905
1105	8	0.13240050986522078
1105	9	0.15389703542846433
1105	10-11	0.16042183187825287
1105	12-13	0.1557245801978624
1105	14-15	0.2616499988105332
1105	16-17	0.24923892908808654
1105	18-19	0.2408001010690839
1105	20-21	0.20265091237316568
1105	22-23	0.22404761263423012
1105	24-25	0.19352745271952898
1105	26-27	0.22207071250415567
1105	28-29	0.2456118031486625
1105	30-31	0.23819785203844646
1105	32-33	0.23641178252464812
1105	34-35	0.23886499276939333
1105	36-37	0.22283239446597491
1105	38-39	0.2037245319652854
1105	40-41	0.20067338983202632
1105	42-43	0.19791076196533908
1105	44-45	0.16606717467934828
1105	46-47	0.2273781536788917
1105	48-49	0.23836418006813886
1105	50-51	0.21346622752423627
1105	52-53	0.25522645299271574
1105	54-55	0.26343068843772244
1105	56-57	0.29304913013690026
1105	58-59	0.2832793151322548
1105	60-61	0.24722436290863214
1105	62-63	0.20401947908999318
1105	64-65	0.16629831760899805
1105	66-67	0.14654975567060546
1105	68-69	0.1716925523852595
1105	70-71	0.15066489813020922
1105	72-73	0.09368334485840535
1105	74-75	0.09083333168214125
1105	76-77	0.10557583020593597
1105	78-79	0.15063637461722124
1105	80-81	0.1284262125888489
1105	82-83	0.14651264806802544
1105	84-85	0.17732611100517914
1105	86-87	0.16049734252292325
1105	88-89	0.10633949021779188
1105	90-91	0.11199064854443463
1105	92-93	0.13932678691655553
1105	94-95	0.233981448558211
1105	96-97	0.16222989194974247
1105	98-99	0.06411767663722401
1105	100	0.062147730684642966
1106	1	0.14852257265167168
1106	2	0.19633964668842196
1106	3	0.1873556868582611
1106	4	0.16343535906487006
1106	5	0.11839630023754211
1106	6	0.10206802304340101
1106	7	0.09528199944463722
1106	8	0.14107300948983692
1106	9	0.1620909669358923
1106	10-11	0.1936022055709259
1106	12-13	0.1466210316315255
1106	14-15	0.20012321770663988
1106	16-17	0.18223878306041286
1106	18-19	0.22516179052208685
1106	20-21	0.1875998856885488
1106	22-23	0.2368878253112996
1106	24-25	0.23998566881154204
1106	26-27	0.21051004741451607
1106	28-29	0.2756096582206169
1106	30-31	0.23441637296846096
1106	32-33	0.20297167902288038
1106	34-35	0.23962065707856084
1106	36-37	0.2954552781940194
1106	38-39	0.2807163349387807
1106	40-41	0.2535599655930483
1106	42-43	0.19434704264322988
1106	44-45	0.18506176146369313
1106	46-47	0.1761320680318974
1106	48-49	0.18207756040815326
1106	50-51	0.18340717588255018
1106	52-53	0.2149797903316255
1106	54-55	0.14493929533275463
1106	56-57	0.18551232942729
1106	58-59	0.20829008588484044
1106	60-61	0.20790307366463168
1106	62-63	0.24802944161018559
1106	64-65	0.14332285879338968
1106	66-67	0.15943087547998402
1106	68-69	0.18740243869031303
1106	70-71	0.16046700983859807
1106	72-73	0.16702189237916087
1106	74-75	0.12005185664504125
1106	76-77	0.17771645682817905
1106	78-79	0.13230411760363125
1106	80-81	0.13063025824014574
1106	82-83	0.15517683183693265
1106	84-85	0.19211409404346114
1106	86-87	0.16236862889545733
1106	88-89	0.1572195844258104
1106	90-91	0.12088731675307685
1106	92-93	0.12045309266611781
1106	94-95	0.10718808034684457
1106	96-97	0.09749003301853776
1106	98-99	0.14651905316770097
1106	100	0.08845306445886081
1107	1	0.11720827158610803
1107	2	0.106948976266942
1107	3	0.15059022244416553
1107	4	0.10021506661966839
1107	5	0.08599953816836603
1107	6	0.08761832584502116
1107	7	0.08129453358534988
1107	8	0.08783394210274054
1107	9	0.04266245327676188
1107	10-11	0.05400932883948428
1107	12-13	0.07098850985058647
1107	14-15	0.10655240218792272
1107	16-17	0.10250098775294703
1107	18-19	0.10289968495072088
1107	20-21	0.09128501752133644
1107	22-23	0.09945336962967843
1107	24-25	0.07988174076207599
1107	26-27	0.12163370443980881
1107	28-29	0.14728799698040973
1107	30-31	0.10024494092312608
1107	32-33	0.03415114639899741
1107	34-35	0.047691269917770285
1107	36-37	0.08073415349359436
1107	38-39	0.11323892075370878
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2216	28-29	-0.39194438658763175
2216	30-31	-0.38599492788335255
2216	32-33	-0.3888776695326044
2216	34-35	-0.41785006145659054
2216	36-37	-0.45736784466114955
2216	38-39	-0.40242281587916295
2216	40-41	-0.4296446953162061
2216	42-43	-0.5534065490967848
2216	44-45	-0.4804232181535255
2216	46-47	-0.43614496652633505
2216	48-49	-0.37681851369544717
2216	50-51	-0.38444140498131674
2216	52-53	-0.3863039979396774
2216	54-55	-0.3820698206491997
2216	56-57	-0.406729021913371
2216	58-59	-0.3752004353918039
2216	60-61	-0.4573959939887544
2216	62-63	-0.37709378520068526
2216	64-65	-0.3838815466254957
2216	66-67	-0.33916535137878867
2216	68-69	-0.40821178223746557
2216	70-71	-0.4056045195326732
2216	72-73	-0.42554938938410203
2216	74-75	-0.40295154253639254
2216	76-77	-0.3499711935788099
2216	78-79	-0.36915214701116383
2216	80-81	-0.3719961360105941
2216	82-83	-0.39783892587763603
2216	84-85	-0.41876332722873855
2216	86-87	-0.3669682057679964
2216	88-89	-0.3498141102996115
2216	90-91	-0.41716828962820074
2216	92-93	-0.3427523778384227
2216	94-95	-0.3245195869985835
2216	96-97	-0.377280587582014
2216	98-99	-0.4188983720283943
2216	100	-0.5635321476118804
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	20.0
8	106.0
9	219.0
10	371.0
11	345.0
12	247.0
13	264.0
14	362.0
15	713.0
16	1388.0
17	2364.0
18	4317.0
19	7063.0
20	10022.0
21	14683.0
22	21298.0
23	25786.0
24	25905.0
25	22330.0
26	20480.0
27	21604.0
28	25895.0
29	32550.0
30	40005.0
31	51359.0
32	66111.0
33	88555.0
34	131608.0
35	211188.0
36	399934.0
37	992041.0
38	1404216.0
39	487786.0
40	1269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24485415672662	19.644643658773482	13.285713521429402	23.8247886630705
2	15.021988508877545	21.650716509720628	38.97387445339042	24.353420528011405
3	18.736627298633472	26.838949330912033	30.517443753497055	23.90697961695744
4	12.205631497870467	18.06327440998637	39.10502491850876	30.626069173634406
5	12.422405576727439	38.657311792017026	35.41742990047188	13.502852730783657
6	29.106059187512233	38.19256672547563	18.520275717167394	14.181098369844742
7	25.39439836027743	33.311010618365955	23.523108022243928	17.771482999112685
8	25.32661544765168	33.66217578278404	21.354924916198673	19.65628385336561
9	26.063848283425394	15.669273819091261	21.34945366519105	36.9174242322923
10-11	24.115791124658198	26.01415716594061	28.757588321189182	21.112463388212007
12-13	26.13166747924876	24.023217557609232	28.153501418269844	21.691613544872162
14-15	23.202484094159512	25.07185273238564	27.002876297796096	24.722786875658752
16-17	21.463875761263786	27.99979573996238	28.040039830707336	22.496288668066498
18-19	21.285221178361567	27.7217589220906	29.723349232383484	21.26967066716435
20-21	22.521030880956523	26.54179245478011	29.06909703689204	21.86807962737133
22-23	22.112214310882187	26.441993093538755	28.557130285350507	22.888662310228554
24-25	22.4715342968409	26.634244438473353	28.660893078381143	22.233328186304607
26-27	22.494185276012455	26.09118022179236	29.30960107285153	22.105033429343656
28-29	21.75493257834896	27.128968148306477	29.21717545678281	21.89892381656175
30-31	22.339983950977533	26.26260820931816	29.092670946405992	22.30473689329832
32-33	22.146762043404415	26.78957528511558	28.983626896084413	22.080035775395586
34-35	22.351026853666287	26.782806446008216	29.211263601885396	21.654903098440098
36-37	22.64478372732586	26.003579690672023	29.189543295812765	22.162093286189354
38-39	22.56417439244562	26.512920717100137	29.052163005333785	21.87074188512046
40-41	22.298821142615154	26.446591889098382	29.159895869239076	22.094691099047388
42-43	22.47370040683318	26.346974563472898	28.369200900647762	22.81012412904616
44-45	22.67636713091116	26.33916049353896	28.47818921425194	22.506283161297937
46-47	22.899590213763876	26.666087927501003	28.328455388937034	22.105866469798084
48-49	23.09767939281758	26.645801984839895	28.08773136282418	22.168787259518353
50-51	22.019316614898017	26.868288611922114	28.312384122378198	22.800010650801674
52-53	22.34893416069562	27.457040336586463	27.131873144915335	23.062152357802578
54-55	22.38946428501951	26.844535563241912	27.43810330673369	23.327896845004883
56-57	23.110356096402658	26.26138474652787	27.526122194445495	23.102136962623977
58-59	23.03685164794547	26.55929617496119	27.705207729449803	22.69864444764354
60-61	22.77320790201728	27.076402427147517	27.97608987828516	22.17429979255004
62-63	22.19771609962282	27.024359058844926	27.723095620972042	23.05482922056021
64-65	21.7490664059465	27.50745677586897	27.496489409134938	23.246987409049584
66-67	22.850908102277074	26.522663526743095	27.16087390648611	23.465554464493717
68-69	22.60785277851947	26.417723636784558	27.495777226251672	23.47864635844431
70-71	22.63443199108241	26.461613769936733	27.52317973232148	23.380774506659378
72-73	22.905371713467275	25.92848877341079	28.215499834090412	22.95063967903152
74-75	22.476462301625418	27.22033260274892	27.35524713701777	22.94795795860789
76-77	22.433475101213123	26.462728268314205	27.35760583925276	23.746190791219917
78-79	22.457558495523013	26.740360658560057	27.47435417956476	23.32772666635217
80-81	22.470252478995544	26.49982011727211	27.72467620197104	23.30525120176131
82-83	22.526120411040083	26.06246430001773	28.15469907209834	23.256716216843852
84-85	22.42752134671708	26.81760932574539	27.31396176029061	23.440907567246917
86-87	22.069341922632116	26.957614086553754	27.488228296636226	23.484815694177907
88-89	22.174373686593565	26.558385156300535	27.235435383442947	24.031805773662956
90-91	22.5323989247168	26.060419632793945	27.33342168390516	24.07375975858409
92-93	22.792222555900988	26.58674182139162	27.304205203524457	23.316830419182935
94-95	21.97416110524134	26.45714612252441	27.833044167585637	23.735648604648617
96-97	21.813090334508402	26.231527482903505	28.246315466444962	23.70906671614313
98-99	22.072556436228613	26.754511068394613	27.263923489653813	23.909009005722957
100	22.076856561199964	25.90020688709334	27.06001962849809	24.96291692320861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	794.0
1	479.0
2	381.5
3	581.0
4	369.5
5	243.5
6	503.0
7	1246.5
8	2411.5
9	2921.5
10	2198.0
11	1487.0
12	1474.0
13	1672.5
14	2003.5
15	2491.0
16	3065.0
17	3575.5
18	4174.0
19	4888.0
20	5749.5
21	6767.0
22	8087.5
23	9861.5
24	11910.5
25	14641.5
26	18777.5
27	23915.5
28	29148.5
29	36076.5
30	44491.5
31	51582.5
32	60889.5
33	71645.0
34	79949.0
35	85797.5
36	92981.5
37	104541.0
38	110777.0
39	112536.0
40	113920.5
41	114562.0
42	116914.5
43	126084.0
44	142303.0
45	156710.0
46	161140.5
47	177233.0
48	216750.0
49	220283.0
50	204492.0
51	186380.0
52	155167.5
53	141079.0
54	132905.0
55	119709.0
56	107574.5
57	95431.5
58	81942.5
59	73775.0
60	63138.0
61	48766.0
62	36988.0
63	28834.5
64	21386.5
65	14193.0
66	9787.0
67	7099.0
68	5165.5
69	3891.5
70	2946.0
71	2155.5
72	1646.0
73	1577.5
74	1288.5
75	905.5
76	683.5
77	426.0
78	232.5
79	91.0
80	52.0
81	45.0
82	24.5
83	10.0
84	6.5
85	4.5
86	5.5
87	4.5
88	1.5
89	1.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006103484457391721
2	0.08919354975981209
3	3.8906673831979097E-4
4	0.0
5	0.039076890529994
6	0.0053496676518971255
7	4.863334228997387E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.2158335572493468E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.836001074796865E-4
24-25	0.0
26-27	0.0
28-29	4.863334228997387E-5
30-31	1.2158335572493467E-4
32-33	0.0019818086983164353
34-35	0.0014954752754166966
36-37	9.361918390819971E-4
38-39	8.875584967920232E-4
40-41	0.0018359086714465137
42-43	7.659751410670886E-4
44-45	5.9575844305218E-4
46-47	4.133834094647779E-4
48-49	8.024501477845689E-4
50-51	9.361918390819971E-4
52-53	0.0014346835975542294
54-55	0.0016778503090040984
56-57	0.0012036752216768533
58-59	0.0032341172622832627
60-61	0.0021398670607588504
62-63	0.0013131002418292946
64-65	0.004997075920294815
66-67	0.0049362842424323485
68-69	0.004352684134952662
70-71	0.0020426003761789026
72-73	0.005483409343194554
74-75	0.004182467436937753
76-77	0.004048725745640325
78-79	9.483501746544905E-4
80-81	8.510834900745427E-5
82-83	1.9453336915989549E-4
84-85	1.0942502015244122E-4
86-87	2.4316671144986936E-5
88-89	1.7021669801490854E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.9180005373984324E-4
98-99	4.863334228997387E-4
100	4.133834094647779E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4112405.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.23619671847266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3394689054059	38.14945272626484
2	15.544195209074442	16.86116058379403
3	5.97278157319495	9.718228690808097
4	2.9490110205449125	6.397725673408708
5	1.657249673183734	4.494145964320875
6	1.0005619904497198	3.2560006165834534
7	0.6320612151348626	2.3996417481519887
8	0.438259741356457	1.90156332367966
9	0.3104060684822012	1.5151720133527524
>10	1.1189543538152686	9.420725394795852
>50	0.02403732858571877	0.8746382393752817
>100	0.010677365332058535	1.0696817951057505
>500	0.0012407638273838112	0.4611243700625918
>1k	6.568749674303273E-4	0.6115073627486817
>5k	1.4597221498451719E-4	0.46912051760322543
>10k+	2.9194442996903437E-4	2.400110979944204
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	23381	0.5685480880409395	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	13218	0.3214177591944373	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13059	0.3175514084823844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10974	0.2668511491450866	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6043	0.14694564372915606	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	5808	0.14123122601008412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.4316671144986936E-5	0.0
2	0.0	0.0	0.0	2.4316671144986936E-5	0.0
3	0.0	0.0	0.0	2.4316671144986936E-5	0.0
4	0.0	0.0	0.0	2.4316671144986936E-5	0.0
5	0.0	0.0	0.0	2.4316671144986936E-5	0.0
6	0.0	0.0	0.0	2.4316671144986936E-5	0.0
7	0.0	0.0	0.0	4.863334228997387E-5	0.0
8	0.0	0.0	0.0	4.863334228997387E-5	0.0
9	0.0	0.0	0.0	4.863334228997387E-5	0.0
10-11	0.0	0.0	0.0	9.726668457994774E-5	0.0
12-13	0.0	0.0	0.0	1.2158335572493467E-4	0.0
14-15	0.0	0.0	0.0	1.3374169129742815E-4	0.0
16-17	0.0	0.0	0.0	1.82375033587402E-4	0.0
18-19	0.0	0.0	0.0	2.7964171816734977E-4	0.0
20-21	0.0	0.0	0.0	3.404333960298171E-4	0.0
22-23	0.0	0.0	0.0	5.228084296172191E-4	0.0
24-25	0.0	0.0	0.0	0.0010456168592344382	0.0
26-27	0.0	0.0	0.0	0.002334400429918746	0.0
28-29	0.0	0.0	0.0	0.006820826256168836	0.0
30-31	0.0	0.0	0.0	0.018006494982862824	0.0
32-33	0.0	0.0	0.0	0.04412259979257879	1.2158335572493468E-5
34-35	0.0	0.0	0.0	0.08335754868501521	2.4316671144986936E-5
36-37	0.0	0.0	0.0	0.1408664759429093	2.4316671144986936E-5
38-39	0.0	0.0	0.0	0.22405380792990964	2.4316671144986936E-5
40-41	0.0	0.0	0.0	0.3144145579046811	2.4316671144986936E-5
42-43	0.0	0.0	0.0	0.3953890728174876	2.4316671144986936E-5
44-45	0.0	0.0	0.0	0.4949050494783466	2.4316671144986936E-5
46-47	0.0	0.0	0.0	0.6126463711623733	2.4316671144986936E-5
48-49	0.0	0.0	0.0	0.733366485061661	2.4316671144986936E-5
50-51	0.0	0.0	0.0	0.8560562493236925	2.4316671144986936E-5
52-53	0.0	0.0	0.0	0.9782961551695419	2.4316671144986936E-5
54-55	0.0	0.0	0.0	1.10740552061385	2.4316671144986936E-5
56-57	0.0	0.0	0.0	1.2601871654178032	2.4316671144986936E-5
58-59	0.0	0.0	0.0	1.4207258283170066	2.4316671144986936E-5
60-61	0.0	0.0	0.0	1.5827478081560549	2.4316671144986936E-5
62-63	0.0	0.0	0.0	1.7445752546259428	2.4316671144986936E-5
64-65	0.0	0.0	0.0	1.9211021288029753	2.4316671144986936E-5
66-67	0.0	0.0	0.0	2.1191978902856112	2.4316671144986936E-5
68-69	0.0	0.0	0.0	2.3316891210860797	2.4316671144986936E-5
70-71	0.0	0.0	0.0	2.559864604775065	2.4316671144986936E-5
72-73	2.4316671144986936E-5	0.0	0.0	2.7731096523810272	2.4316671144986936E-5
74-75	2.4316671144986936E-5	0.0	0.0	2.991436884256293	2.4316671144986936E-5
76-77	2.4316671144986936E-5	0.0	0.0	3.229168819705258	2.4316671144986936E-5
78-79	2.4316671144986936E-5	0.0	0.0	3.497089902380724	2.4316671144986936E-5
80-81	2.4316671144986936E-5	0.0	0.0	3.774154053406705	2.4316671144986936E-5
82-83	2.4316671144986936E-5	0.0	0.0	4.055449305211914	2.4316671144986936E-5
84-85	2.4316671144986936E-5	0.0	0.0	4.330057472452252	2.4316671144986936E-5
86-87	2.4316671144986936E-5	0.0	0.0	4.6204350009301125	2.4316671144986936E-5
88	2.4316671144986936E-5	0.0	0.0	4.856331027707631	2.4316671144986936E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1730	0.0	26.929283	1
GAGTACT	13295	0.0	22.269848	12-13
GTACTTT	14355	0.0	20.494446	14-15
CTAGTAC	2640	0.0	19.767803	3
AGTACTT	14090	0.0	19.679136	12-13
TCTAACG	730	0.0	19.337357	2
CATGGGG	7840	0.0	19.069988	4
CTGTGCG	2490	0.0	18.307856	9
TAGTACT	2705	0.0	18.249937	4
CGACGAG	1020	0.0	17.96945	24-25
GCATCGT	2135	0.0	17.609911	8
TAGGCAT	2635	0.0	17.485773	5
TGATCGC	1000	0.0	17.388687	9
ACTTTTT	17035	0.0	17.353064	16-17
GTATAAG	2405	0.0	17.218817	1
ATAACGC	415	4.5474735E-11	16.993486	3
GTGATCG	1045	0.0	16.63989	8
TACTTTT	16560	0.0	16.602007	14-15
GTATCGG	965	0.0	16.580107	1
GTACCCG	515	0.0	16.447542	1
>>END_MODULE