FastQCFastQC Report
Mon 16 Jan 2017
ERR1146576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534063
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48350.039.5844761
GTATCAA76100.035.284051
CAACGCA79650.032.1540645
ATCAACG79800.032.035093
AACGCAG96750.027.4421316
TATCAAC97600.027.3481832
GTACTAG23050.026.9296071
GTACATG163350.025.8226181
ACGCAGA100500.025.4345747
TACATGG165200.024.9470652
CGCAGAG102600.024.7321558
TCAACGC110550.023.8470154
ACATGGG170150.023.4479923
TAGTACT32750.022.2407954
CATGGGG144500.022.0166264
CTAGTAC32900.021.8537243
GCAGAGT116350.021.2035459
GTGATCG22050.020.671828
GTATAAG28450.020.1652951
TAGGCAT39650.020.0294325
Produced by FastQC (version 0.11.5)