##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146576_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4534063
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420984666512133	31.0	31.0	34.0	30.0	34.0
2	31.613638363648676	33.0	31.0	34.0	30.0	34.0
3	31.70357976940329	33.0	31.0	34.0	30.0	34.0
4	35.2542809837446	37.0	35.0	37.0	33.0	37.0
5	35.278753735887655	37.0	35.0	37.0	33.0	37.0
6	35.439162623016045	37.0	35.0	37.0	33.0	37.0
7	35.40173614702751	37.0	35.0	37.0	33.0	37.0
8	35.33161074294733	37.0	35.0	37.0	33.0	37.0
9	36.993196168646094	39.0	38.0	39.0	33.0	39.0
10-11	36.947750284898994	39.0	38.0	39.0	33.0	39.0
12-13	36.89552141203154	39.0	38.0	39.0	33.0	39.0
14-15	38.33332741516825	41.0	39.0	41.0	34.0	41.0
16-17	38.35198341972752	41.0	39.0	41.0	33.5	41.0
18-19	38.385528829220064	41.0	39.0	41.0	33.5	41.0
20-21	38.4716829033915	41.0	39.0	41.0	34.0	41.0
22-23	38.496351616640524	41.0	39.0	41.0	34.0	41.0
24-25	38.55106203861746	41.0	39.0	41.0	34.0	41.0
26-27	38.5785286838758	41.0	39.0	41.0	34.0	41.0
28-29	38.548904150648106	41.0	39.0	41.0	34.0	41.0
30-31	38.534486508017196	40.0	39.0	41.0	34.0	41.0
32-33	38.54879111737088	40.0	38.0	41.0	33.5	41.0
34-35	38.495673416977226	40.0	38.0	41.0	33.0	41.0
36-37	38.40514004767909	40.0	38.0	41.0	33.0	41.0
38-39	38.357918824683296	40.0	38.0	41.0	33.0	41.0
40-41	38.28566839499142	40.0	38.0	41.0	33.0	41.0
42-43	38.160497769881005	40.0	38.0	41.0	33.0	41.0
44-45	38.02402458898344	40.0	38.0	41.0	33.0	41.0
46-47	37.90920604764425	40.0	37.0	41.0	33.0	41.0
48-49	37.77471662833092	40.0	37.0	41.0	32.0	41.0
50-51	37.62440100633802	40.0	36.5	41.0	32.0	41.0
52-53	37.46703497503233	40.0	36.0	41.0	32.0	41.0
54-55	37.262519620922774	39.5	35.5	41.0	31.0	41.0
56-57	37.092107454175206	39.0	35.0	41.0	31.0	41.0
58-59	36.82777091981298	39.0	35.0	41.0	31.0	41.0
60-61	36.620612130885696	38.5	35.0	41.0	31.0	41.0
62-63	36.352761750332974	37.5	35.0	40.0	31.0	41.0
64-65	36.07790165244727	37.0	35.0	40.0	31.0	41.0
66-67	35.80378569949293	36.5	35.0	40.0	30.0	41.0
68-69	35.49917369917445	36.0	35.0	39.0	30.0	41.0
70-71	35.25324207008151	35.5	35.0	39.0	30.0	41.0
72-73	34.95539144471526	35.0	34.5	38.5	30.0	40.5
74-75	34.649876501495456	35.0	34.0	37.0	30.0	40.0
76-77	34.37302778986529	35.0	34.0	37.0	29.5	39.0
78-79	34.1221475087576	35.0	34.0	36.5	29.0	39.0
80-81	33.89208641344419	35.0	34.0	36.0	29.0	38.5
82-83	33.666174356201054	35.0	34.0	36.0	29.0	37.0
84-85	33.47988779600107	35.0	34.0	35.0	29.0	37.0
86-87	33.31743383362781	35.0	34.0	35.0	29.0	36.5
88-89	33.21184079268418	35.0	34.0	35.0	29.0	36.0
90-91	33.0731405805345	35.0	34.0	35.0	28.5	36.0
92-93	32.99817029450186	35.0	34.0	35.0	28.0	36.0
94-95	32.93064917712877	35.0	34.0	35.0	28.0	35.5
96-97	32.86894899784145	35.0	34.0	35.0	28.0	35.0
98-99	32.81402309584141	35.0	34.0	35.0	28.0	35.0
100	31.903914877230424	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23599337673843834
1101	2	0.2654706727885099
1101	3	0.2869085171585688
1101	4	0.35756448689890163
1101	5	0.2201168633598698
1101	6	0.2938841516707953
1101	7	0.3604330800027711
1101	8	0.41095559081321653
1101	9	0.5182523273601802
1101	10-11	0.48020183286312346
1101	12-13	0.4573792977440476
1101	14-15	0.5532161341325761
1101	16-17	0.46616432087084547
1101	18-19	0.44647175104466186
1101	20-21	0.46660058064282595
1101	22-23	0.4277764935898958
1101	24-25	0.445216707817913
1101	26-27	0.4315037141615008
1101	28-29	0.45861210698393506
1101	30-31	0.431280328485137
1101	32-33	0.47167013115912226
1101	34-35	0.48065934188288395
1101	36-37	0.4439052188416923
1101	38-39	0.4386578612240086
1101	40-41	0.4741521062934453
1101	42-43	0.43682131276893443
1101	44-45	0.40735463634923974
1101	46-47	0.4359307932485521
1101	48-49	0.37047203933985173
1101	50-51	0.33957707712590945
1101	52-53	0.36372491234818227
1101	54-55	0.3729706953700429
1101	56-57	0.3682135869882259
1101	58-59	0.3012745826848757
1101	60-61	0.32510322157651217
1101	62-63	0.3648319528629642
1101	64-65	0.26118010125567537
1101	66-67	0.30980911038547987
1101	68-69	0.24687293141055022
1101	70-71	0.22337569306613858
1101	72-73	0.24219821790158846
1101	74-75	0.2508462836177543
1101	76-77	0.2521660039774787
1101	78-79	0.2834686567475231
1101	80-81	0.2533045050251417
1101	82-83	0.2166588297148735
1101	84-85	0.2892048041931119
1101	86-87	0.3035265336891584
1101	88-89	0.23972621680910322
1101	90-91	0.28091271537464024
1101	92-93	0.24803265224628746
1101	94-95	0.24017902144191083
1101	96-97	0.2107013181146442
1101	98-99	0.21635761372318996
1101	100	0.16830528313941784
1102	1	0.22240473750287038
1102	2	0.20795095028799437
1102	3	0.23148099074023776
1102	4	0.2796039721756216
1102	5	0.23213072323068218
1102	6	0.29372531442988503
1102	7	0.2842712522920934
1102	8	0.24993968695127933
1102	9	0.40163973919418083
1102	10-11	0.4255469073983136
1102	12-13	0.37457563164962693
1102	14-15	0.4613501867225551
1102	16-17	0.47762917278218
1102	18-19	0.41076578118094176
1102	20-21	0.428486565207173
1102	22-23	0.38386259042271575
1102	24-25	0.4335163689986956
1102	26-27	0.41716424801573737
1102	28-29	0.46281462320747835
1102	30-31	0.49235135969376387
1102	32-33	0.43028774371707357
1102	34-35	0.44986713170420956
1102	36-37	0.5025555553954035
1102	38-39	0.48323901925070345
1102	40-41	0.4634162443182248
1102	42-43	0.4375492958128717
1102	44-45	0.4294599472020266
1102	46-47	0.32279846496142994
1102	48-49	0.3659271797761363
1102	50-51	0.37410554951944874
1102	52-53	0.38700048363363493
1102	54-55	0.44465915286156843
1102	56-57	0.3552741414837399
1102	58-59	0.40028203872144985
1102	60-61	0.37550339828609225
1102	62-63	0.3658244968263986
1102	64-65	0.29904572897742554
1102	66-67	0.2869773897577872
1102	68-69	0.27006360909932425
1102	70-71	0.2459896732605671
1102	72-73	0.3164803037794215
1102	74-75	0.26795092740447757
1102	76-77	0.28699981642367334
1102	78-79	0.280450308654828
1102	80-81	0.3022562673381728
1102	82-83	0.295647947727673
1102	84-85	0.31266488846998897
1102	86-87	0.3165499288288913
1102	88-89	0.25212842240641464
1102	90-91	0.32263524358020845
1102	92-93	0.31163551383385624
1102	94-95	0.30933105460893984
1102	96-97	0.2676814722698353
1102	98-99	0.282851262750782
1102	100	0.21241471218121433
1103	1	0.16667533040666882
1103	2	0.21719788148798003
1103	3	0.2280625647047465
1103	4	0.1489137242696401
1103	5	0.28147447127660996
1103	6	0.269053559967837
1103	7	0.28019051854195
1103	8	0.2947632550867212
1103	9	0.42572322404109286
1103	10-11	0.3996559005674385
1103	12-13	0.4130183231715776
1103	14-15	0.4640661624645972
1103	16-17	0.45858295387832726
1103	18-19	0.43822819212449815
1103	20-21	0.42931431534444897
1103	22-23	0.42958963559295427
1103	24-25	0.3738243393958882
1103	26-27	0.3823260342654393
1103	28-29	0.4372788916494841
1103	30-31	0.4099270779231077
1103	32-33	0.41203303304673256
1103	34-35	0.39832437438788304
1103	36-37	0.4081136675619561
1103	38-39	0.3476257458604266
1103	40-41	0.3848983017512282
1103	42-43	0.3755030070854488
1103	44-45	0.3432194452709396
1103	46-47	0.311881252768174
1103	48-49	0.26258064871068143
1103	50-51	0.29467447537338387
1103	52-53	0.322638867806063
1103	54-55	0.3461769279237217
1103	56-57	0.30652724154894173
1103	58-59	0.3566795462588672
1103	60-61	0.3084649330274445
1103	62-63	0.3156289370080927
1103	64-65	0.2685080318379107
1103	66-67	0.23063725053866335
1103	68-69	0.2292487990403771
1103	70-71	0.18895779939542479
1103	72-73	0.22399283346490506
1103	74-75	0.24044251539707062
1103	76-77	0.20739360047537048
1103	78-79	0.24485642505671734
1103	80-81	0.2765074034956996
1103	82-83	0.2170202781266184
1103	84-85	0.22304835979455362
1103	86-87	0.17626375139833073
1103	88-89	0.12726204667929153
1103	90-91	0.21293025357370254
1103	92-93	0.206210804752466
1103	94-95	0.15879624964806993
1103	96-97	0.1973547529270121
1103	98-99	0.08638018101021316
1103	100	0.1220162252007384
1104	1	0.11464094791828572
1104	2	0.0858332476992274
1104	3	0.0891448103158261
1104	4	0.16535719598017096
1104	5	0.14191418088744712
1104	6	0.17451107870060412
1104	7	0.1554020363445474
1104	8	0.21047527056843762
1104	9	0.2336179995973353
1104	10-11	0.2430316277356397
1104	12-13	0.21793249901863732
1104	14-15	0.30943143586379307
1104	16-17	0.31587969534230353
1104	18-19	0.26536995928824325
1104	20-21	0.24179862181849643
1104	22-23	0.27398596436943734
1104	24-25	0.25261122273435177
1104	26-27	0.27676310351890265
1104	28-29	0.247519337364011
1104	30-31	0.24052702115057656
1104	32-33	0.2359417666882706
1104	34-35	0.2685974258579762
1104	36-37	0.24985208561441397
1104	38-39	0.23826565053072102
1104	40-41	0.2632079848782496
1104	42-43	0.20602508869144742
1104	44-45	0.23486569990544126
1104	46-47	0.17169898886817947
1104	48-49	0.18979349020264635
1104	50-51	0.16986693525898033
1104	52-53	0.15558046899875677
1104	54-55	0.2016825595381988
1104	56-57	0.18414745437572577
1104	58-59	0.21390193677029856
1104	60-61	0.17822137284805706
1104	62-63	0.14336572076059184
1104	64-65	0.1341328740667791
1104	66-67	0.14194287499805824
1104	68-69	0.17724567822288861
1104	70-71	0.12130875561805254
1104	72-73	0.1553619726121056
1104	74-75	0.1407121013354029
1104	76-77	0.1422350837430173
1104	78-79	0.12241290252507042
1104	80-81	0.11949231798416804
1104	82-83	0.09652509325980674
1104	84-85	0.09867839948517343
1104	86-87	0.11864172138245266
1104	88-89	0.08791609934534961
1104	90-91	0.12467471164845989
1104	92-93	0.08857372232564131
1104	94-95	0.04065212632395543
1104	96-97	0.013331464486782352
1104	98-99	0.03294027714235881
1104	100	0.05602133339119675
1105	1	8.165354330671448E-4
1105	2	0.05993103364530583
1105	3	0.1107107350201666
1105	4	0.1340958450500409
1105	5	0.14179697375736566
1105	6	0.1763972522285684
1105	7	0.18320203230034338
1105	8	0.22802301281460302
1105	9	0.283061621469912
1105	10-11	0.29782193556724224
1105	12-13	0.2615398236779001
1105	14-15	0.3588773981343323
1105	16-17	0.3017326449552229
1105	18-19	0.2290190853274794
1105	20-21	0.22410146856623925
1105	22-23	0.20640304126106201
1105	24-25	0.22241840389913392
1105	26-27	0.21970148627020336
1105	28-29	0.27681671993781265
1105	30-31	0.2078844609574091
1105	32-33	0.20634743442066394
1105	34-35	0.19260947834580122
1105	36-37	0.20402025949736924
1105	38-39	0.1770927029848508
1105	40-41	0.18289900741122977
1105	42-43	0.1665870425829894
1105	44-45	0.12962933094488704
1105	46-47	0.13006181121587446
1105	48-49	0.10459841709609918
1105	50-51	0.11590241344534036
1105	52-53	0.05200391027498341
1105	54-55	0.08404723023858907
1105	56-57	0.04585399393443623
1105	58-59	0.09775350910582148
1105	60-61	0.10988119057412149
1105	62-63	0.0805229460746304
1105	64-65	0.07664024912884315
1105	66-67	0.06558561268700913
1105	68-69	0.07058625368367188
1105	70-71	0.08374621082283795
1105	72-73	0.06331533765502684
1105	74-75	0.08675188585402793
1105	76-77	0.06337265945021642
1105	78-79	0.1418676010331339
1105	80-81	0.1492926988886154
1105	82-83	0.1928540011814377
1105	84-85	0.17906713771484561
1105	86-87	0.14703112316174582
1105	88-89	0.08286467799357666
1105	90-91	0.10690528690704326
1105	92-93	0.1517736417410589
1105	94-95	0.13572938941388912
1105	96-97	0.08627708187627547
1105	98-99	0.10282418591401665
1105	100	0.0678970743611913
1106	1	0.05647013348781016
1106	2	0.08852863091639307
1106	3	0.11031836026051067
1106	4	0.04187601852500222
1106	5	0.0963899452267043
1106	6	0.11434106988365755
1106	7	0.10800367046820725
1106	8	0.11428977423400966
1106	9	0.08825774097515904
1106	10-11	0.10352462264521733
1106	12-13	0.130385140577296
1106	14-15	0.1413272151364211
1106	16-17	0.12290885403051988
1106	18-19	0.095347799754677
1106	20-21	0.12804741621123839
1106	22-23	0.15582498216095786
1106	24-25	0.15515461917580353
1106	26-27	0.16126867731362182
1106	28-29	0.176655554802025
1106	30-31	0.10247875825216823
1106	32-33	0.12279842301843757
1106	34-35	0.13513518042395134
1106	36-37	0.12740573287894819
1106	38-39	0.1262436906456088
1106	40-41	0.15370782554143858
1106	42-43	0.10684508485745425
1106	44-45	0.030098166481614896
1106	46-47	0.02545600327518116
1106	48-49	0.07571678483164135
1106	50-51	0.08736543608900149
1106	52-53	0.12062997541594456
1106	54-55	0.17022019755655293
1106	56-57	0.09112083485664613
1106	58-59	0.14751192103069144
1106	60-61	0.07305693488375908
1106	62-63	0.07942702470864305
1106	64-65	0.0338416159748931
1106	66-67	0.08127049326525793
1106	68-69	0.038623245354678204
1106	70-71	-0.006591127906581562
1106	72-73	0.014738267836641228
1106	74-75	0.02925525291328057
1106	76-77	8.706073149085114E-4
1106	78-79	0.02901177439773761
1106	80-81	0.04323442688919954
1106	82-83	0.02500227206812866
1106	84-85	0.09489689872132345
1106	86-87	0.04687917235683159
1106	88-89	0.013323928290176923
1106	90-91	0.09628013816373482
1106	92-93	0.07061792949054535
1106	94-95	0.0656841356287643
1106	96-97	0.043615897987379526
1106	98-99	0.008190113856962
1106	100	-0.017064351205327455
1107	1	0.006665306014241423
1107	2	0.06126579870151261
1107	3	0.10283037921126237
1107	4	0.11209793108177024
1107	5	0.09984937026052876
1107	6	0.11716118222027205
1107	7	0.08740573528815787
1107	8	0.08468178148539351
1107	9	0.10512008715691934
1107	10-11	0.16456196489479424
1107	12-13	0.19124341926147537
1107	14-15	0.19871259737387703
1107	16-17	0.16483330330503776
1107	18-19	0.16261538076331306
1107	20-21	0.18298422429997885
1107	22-23	0.21642010958954216
1107	24-25	0.1770907509175217
1107	26-27	0.22728148553707683
1107	28-29	0.19709171231568234
1107	30-31	0.20464241684648954
1107	32-33	0.19135072535652853
1107	34-35	0.17670147842536466
1107	36-37	0.19556288584310266
1107	38-39	0.19291500470830414
1107	40-41	0.19767607887739302
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2216	52-53	-0.3195881295260108
2216	54-55	-0.2720086442351928
2216	56-57	-0.2832855829066361
2216	58-59	-0.27370863074437324
2216	60-61	-0.2519005600136026
2216	62-63	-0.2774156029385395
2216	64-65	-0.2946665137600064
2216	66-67	-0.3070249608190849
2216	68-69	-0.26778146814672965
2216	70-71	-0.283281289040481
2216	72-73	-0.2814402251900887
2216	74-75	-0.2703194607098567
2216	76-77	-0.2446412271606846
2216	78-79	-0.2520241490078732
2216	80-81	-0.23371938355630562
2216	82-83	-0.2540219487392861
2216	84-85	-0.24119364627728146
2216	86-87	-0.2298982956781188
2216	88-89	-0.2722288652790752
2216	90-91	-0.23710028960599772
2216	92-93	-0.19680095778875994
2216	94-95	-0.19596827610467216
2216	96-97	-0.21025662322459482
2216	98-99	-0.20934945139394756
2216	100	-0.26424922709854215
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	122.0
1	60.0
2	59.0
3	52.0
4	45.0
5	67.0
6	163.0
7	965.0
8	2594.0
9	3093.0
10	2853.0
11	3281.0
12	4198.0
13	5107.0
14	6539.0
15	7515.0
16	8191.0
17	9094.0
18	10692.0
19	12125.0
20	13863.0
21	15619.0
22	17622.0
23	21452.0
24	26776.0
25	35174.0
26	39709.0
27	37454.0
28	40656.0
29	50411.0
30	57201.0
31	63400.0
32	79486.0
33	107459.0
34	167867.0
35	270872.0
36	532085.0
37	1192960.0
38	1286437.0
39	400180.0
40	565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37574100111648	20.124443828468436	13.010303449350404	24.48951172106468
2	15.545797357698829	20.720134709009834	39.247476191232444	24.48659174205889
3	17.972257018407017	26.125284352657513	30.220271430093415	25.682187198842055
4	12.73222520192393	17.609610648225555	38.87573291030294	30.782431239547574
5	13.66905332256912	38.241175769928134	34.80545004565968	13.284320861843064
6	30.08218312689394	37.98676705634059	18.36192250901835	13.569127307747115
7	26.573830519958584	32.774885761560924	23.09491219332951	17.556371525150983
8	26.022489565127632	34.44568793401437	20.80657527091849	18.725247229939505
9	26.662681734416026	15.199090376719468	20.8365490339664	37.3016788548981
10-11	25.016181347551964	26.119435494018827	28.21768949656572	20.646693661863488
12-13	25.36206870692363	24.1030939113434	27.725369049593628	22.80946833213934
14-15	23.01973045703194	25.288071289924126	25.119220928180695	26.572977324863245
16-17	22.653280785330526	27.973231178310108	26.07152211830138	23.30196591805798
18-19	22.510893646840383	27.914921089643702	27.34519033539893	22.228994928116986
20-21	23.854754881520435	27.16170354539332	26.32976649716411	22.65377507592214
22-23	24.2089471158814	26.72657995256445	26.00396365943407	23.060509272120076
24-25	23.60190238278671	27.020939841323997	26.44904720953935	22.928110566349932
26-27	23.355218791166195	27.258384600666215	26.03994523397148	23.346451374196114
28-29	23.135229632655467	27.353637807439707	26.248901169009496	23.262231390895334
30-31	23.403406585032116	27.078461212523525	26.28148931751727	23.236642884927093
32-33	23.527856060541726	27.432324484700406	25.670909909243218	23.36890954551465
34-35	23.745908551395033	27.243715232750738	26.270160565438804	22.740215650415426
36-37	23.638428582789086	26.90116826895874	26.17694100176132	23.28346214649085
38-39	23.605707331971317	26.958003265448205	26.4182325968409	23.018056805739583
40-41	23.44752455416033	26.516342380864565	26.65132298195288	23.384810083022227
42-43	23.27221626892317	26.707482460441202	26.39663752277068	23.62366374786495
44-45	23.287647962689853	27.158465545141375	26.33713266480101	23.21675382736776
46-47	23.431683924975335	26.863614943512648	26.123878592343274	23.580822539168743
48-49	23.67825489934793	26.66648869842855	26.266865432184833	23.38839097003868
50-51	23.513921427731486	26.74356778876309	26.06457814547313	23.67793263803229
52-53	23.345339815281896	26.912238989183134	26.058375262992257	23.684045932542713
54-55	23.32844888745281	26.917705783528845	26.077535494602273	23.67630983441607
56-57	23.660914437615393	26.74814088827855	25.949236939001015	23.64170773510504
58-59	23.301070158670484	26.732808050106655	26.528270157258433	23.43785163396443
60-61	23.359201672324296	27.05711983861881	26.1478282520564	23.435850237000494
62-63	23.570305351216504	26.728140286934504	25.926508540689134	23.775045821159864
64-65	23.086991385880708	26.805060473793656	26.11664356131897	23.991304579006663
66-67	23.629416259807616	26.555822878663538	26.031209399820337	23.78355146170851
68-69	23.122116356073583	26.607609080095664	26.34068826406704	23.929586299763717
70-71	23.246370307512503	26.579202788182787	26.099266791416486	24.07516011288822
72-73	23.6262386409079	26.569919607275335	26.282171568830236	23.52167018298653
74-75	23.51119619382694	26.87568611361749	26.248722646881955	23.364395045673614
76-77	23.41931750135581	26.74301564796686	26.005196867141212	23.83246998353612
78-79	23.261592024927342	26.803037482025143	26.070264524815645	23.86510596823187
80-81	23.245075144989503	26.818322079080325	26.457642457307955	23.478960318622214
82-83	23.708326796944345	26.22673955650518	26.96275350433189	23.102180142218586
84-85	23.87068156513572	26.651077776808236	26.05675916488941	23.42148149316663
86-87	23.201558585795855	26.994946907058587	26.334692422324153	23.468802084821405
88-89	23.63748518948687	26.396628216601876	26.139373974598445	23.82651261931281
90-91	23.585355236732195	26.353741112380195	26.358175683591355	23.70272796729625
92-93	23.866111917876704	26.3181020107658	26.429034118462607	23.38675195289489
94-95	23.043407417099107	26.44284406379111	26.716677060523804	23.797071458585986
96-97	23.27808773343382	26.430994222234382	26.450354193711796	23.840563850620004
98-99	23.131185583585292	26.82799407368709	26.260480447877644	23.780339894849973
100	23.62849771167174	26.269412734113796	26.045157667967928	24.056931886246534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1170.0
1	873.5
2	680.0
3	761.5
4	735.0
5	652.0
6	611.5
7	622.0
8	636.0
9	677.0
10	610.0
11	530.5
12	535.5
13	538.0
14	568.0
15	709.0
16	792.5
17	864.5
18	1107.0
19	1502.0
20	2059.5
21	2796.5
22	4116.0
23	5952.0
24	7838.0
25	10626.5
26	14674.5
27	19108.0
28	23908.5
29	31829.0
30	42428.0
31	51965.0
32	63682.0
33	73253.5
34	78905.0
35	87328.0
36	98570.0
37	113745.5
38	120499.5
39	118933.0
40	119298.0
41	119395.0
42	119321.0
43	125264.0
44	137411.5
45	157661.5
46	174331.5
47	185049.0
48	192589.5
49	188904.0
50	185810.0
51	190332.5
52	194065.5
53	195576.0
54	191021.0
55	171384.5
56	155343.0
57	137158.0
58	118627.0
59	108954.5
60	90454.0
61	69489.0
62	54916.5
63	43774.5
64	32654.0
65	22146.0
66	15459.5
67	11743.5
68	9227.5
69	6783.0
70	5269.0
71	4261.0
72	3305.5
73	2615.0
74	1970.0
75	1569.0
76	1258.5
77	879.0
78	556.5
79	423.5
80	306.0
81	173.5
82	105.0
83	74.0
84	57.0
85	38.5
86	32.0
87	30.5
88	28.5
89	23.5
90	24.0
91	21.0
92	10.5
93	10.5
94	12.0
95	9.0
96	6.5
97	8.5
98	8.5
99	7.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06312219305289758
2	0.009682265111887505
3	0.011468742273761965
4	0.009373491281440069
5	0.011534908094572131
6	0.01166723973619246
7	0.0027569092004235494
8	0.005006547107969166
9	0.007013577005877509
10-11	0.006473222802594494
12-13	0.005624094768864041
14-15	0.010211591678368827
16-17	0.009494795286258705
18-19	0.0075539312091605255
20-21	0.006759941359438544
22-23	0.00267971574281169
24-25	0.003749396512576027
26-27	0.005083740565581025
28-29	0.005899785688906396
30-31	0.008877247625363828
32-33	0.010200564041567134
34-35	0.011512852820968743
36-37	0.018030186170770013
38-39	0.018559512737251337
40-41	0.018063269081175098
42-43	0.024161552232511988
44-45	0.027524981457028717
46-47	0.03014955901583194
48-49	0.03129643324320813
50-51	0.03189192563049962
52-53	0.030270863020650574
54-55	0.03635811853518577
56-57	0.03950099502366861
58-59	0.036413256719194244
60-61	0.03724032947932131
62-63	0.03298366167386734
64-65	0.03446136500529437
66-67	0.03701977674328742
68-69	0.04014059795816688
70-71	0.039523050297272
72-73	0.03585084724230784
74-75	0.03840925898030089
76-77	0.037868904777017875
78-79	0.03728444002652808
80-81	0.04244537404972097
82-83	0.03846439716430936
84-85	0.03443930973169098
86-87	0.03291749585305718
88-89	0.0306016921247014
90-91	0.03295057876346226
92-93	0.03442828209488929
94-95	0.03834309315949073
96-97	0.03341373950913342
98-99	0.030800189587131894
100	0.028186639665130367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4534063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.44589248577486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.87287784567603	32.397162503259764
2	15.208346128184825	14.735638028249845
3	6.701547076742603	9.73987287504693
4	3.5272091385425126	6.835151788026924
5	2.156224535686207	5.22301110155219
6	1.3531573628731122	3.9332949670851254
7	0.9082121993309844	3.07994053941406
8	0.655464800224528	2.5403661791909906
9	0.47624470529654467	2.076488981074434
>10	2.067204802558056	16.321546402658406
>50	0.056961485642587906	1.814403403338236
>100	0.01646158827074932	1.237308016456799
>500	0.0	0.0
>1k	8.833097119811609E-5	0.06581521464627475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.4110547206776795E-5
2	0.0	0.0	0.0	0.0	4.4110547206776795E-5
3	0.0	0.0	0.0	0.0	4.4110547206776795E-5
4	0.0	0.0	0.0	0.0	4.4110547206776795E-5
5	0.0	0.0	0.0	0.0	4.4110547206776795E-5
6	0.0	0.0	0.0	0.0	6.616582081016519E-5
7	0.0	0.0	0.0	0.0	6.616582081016519E-5
8	0.0	0.0	0.0	0.0	6.616582081016519E-5
9	0.0	0.0	0.0	0.0	6.616582081016519E-5
10-11	0.0	0.0	0.0	0.0	6.616582081016519E-5
12-13	0.0	0.0	0.0	1.1027636801694199E-5	6.616582081016519E-5
14-15	0.0	0.0	0.0	2.2055273603388397E-5	8.822109441355359E-5
16-17	0.0	0.0	0.0	2.2055273603388397E-5	1.2130400481863617E-4
18-19	0.0	0.0	0.0	2.2055273603388397E-5	1.5438691522371876E-4
20-21	0.0	0.0	0.0	4.4110547206776795E-5	1.5438691522371876E-4
22-23	0.0	0.0	0.0	1.6541455202541297E-4	2.4260800963727235E-4
24-25	0.0	0.0	0.0	4.190501984643795E-4	3.5288437765421436E-4
26-27	0.0	0.0	0.0	0.001157901864177891	3.749396512576027E-4
28-29	0.0	0.0	0.0	0.00425666780545396	3.749396512576027E-4
30-31	0.0	0.0	0.0	0.013376523440455063	4.411054720677679E-4
32-33	0.0	0.0	0.0	0.035718515600687506	5.403542032830157E-4
34-35	0.0	0.0	0.0	0.07036735043161069	5.954923872914867E-4
36-37	0.0	0.0	0.0	0.12465640640635121	5.954923872914867E-4
38-39	0.0	0.0	0.0	0.19548691758363307	6.285752976965693E-4
40-41	0.0	0.0	0.0	0.2699234659950689	6.616582081016519E-4
42-43	0.0	0.0	0.0	0.34698459196530795	6.726858449033461E-4
44-45	0.0	0.0	0.0	0.44183329609667976	6.947411185067345E-4
46-47	0.0	0.0	0.0	0.5533998976194199	7.939898497219823E-4
48-49	0.0	0.0	0.0	0.672035214332046	9.263214913423127E-4
50-51	0.0	0.0	0.0	0.793890600990767	9.263214913423127E-4
52-53	0.0	0.0	0.0	0.9126803046186169	9.814596753507836E-4
54-55	0.0	0.0	0.0	1.0366419699064613	0.001058653132962643
56-57	0.0	0.0	0.0	1.1852834863564974	0.0010807084065660314
58-59	0.0	0.0	0.0	1.34711405642136	0.0010917360433677255
60-61	0.0	0.0	0.0	1.5099040308879697	0.0012130400481863619
62-63	0.0	0.0	0.0	1.6701907318005946	0.0013453716898066922
64-65	0.0	0.0	0.0	1.8372042911622535	0.0013563993266083865
66-67	0.0	0.0	0.0	2.02685758887779	0.0013784546002117747
68-69	0.0	0.0	0.0	2.230560095878685	0.001389482237013469
70-71	0.0	0.0	0.0	2.4493042994770917	0.0014115375106168574
72-73	0.0	0.0	0.0	2.6679492543442827	0.0016982560674609064
74-75	0.0	0.0	0.0	2.8895496158743272	0.0016982560674609064
76-77	0.0	0.0	0.0	3.1295661308632017	0.0016982560674609064
78-79	0.0	0.0	0.0	3.390402824133674	0.0016982560674609064
80-81	0.0	0.0	0.0	3.6646491237550074	0.0017533942514693776
82-83	0.0	0.0	1.1027636801694199E-5	3.952051394080762	0.0019518917138998731
84-85	0.0	0.0	2.2055273603388397E-5	4.234932333317821	0.0019739469875032613
86-87	0.0	0.0	2.2055273603388397E-5	4.532854969152392	0.0019849746243049554
88	0.0	0.0	2.2055273603388397E-5	4.769474972006344	0.0020290851715117323
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4835	0.0	39.584476	1
GTATCAA	7610	0.0	35.28405	1
CAACGCA	7965	0.0	32.154064	5
ATCAACG	7980	0.0	32.03509	3
AACGCAG	9675	0.0	27.442131	6
TATCAAC	9760	0.0	27.348183	2
GTACTAG	2305	0.0	26.929607	1
GTACATG	16335	0.0	25.822618	1
ACGCAGA	10050	0.0	25.434574	7
TACATGG	16520	0.0	24.947065	2
CGCAGAG	10260	0.0	24.732155	8
TCAACGC	11055	0.0	23.847015	4
ACATGGG	17015	0.0	23.447992	3
TAGTACT	3275	0.0	22.240795	4
CATGGGG	14450	0.0	22.016626	4
CTAGTAC	3290	0.0	21.853724	3
GCAGAGT	11635	0.0	21.203545	9
GTGATCG	2205	0.0	20.67182	8
GTATAAG	2845	0.0	20.165295	1
TAGGCAT	3965	0.0	20.029432	5
>>END_MODULE