FastQCFastQC Report
Mon 16 Jan 2017
ERR1146576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534063
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160360.35367836750393633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145880.3217423313262299No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78190.17245018430489387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83300.058.7110141
GTATCAA164750.046.570531
ATCAACG203850.036.7709353
CAACGCA207350.036.127185
TATCAAC217850.035.1304132
AACGCAG228550.033.335256
TCAACGC233350.032.343694
ACGCAGA244800.030.5629487
CGCAGAG250600.029.8555878
GTGGTAT21250.029.2391011
GCAGAGT271900.027.2920749
GTACATG154450.024.8685971
TACATGG155450.024.222532
AGAGTAC254100.023.30394710-11
GTACTAG20550.023.1344181
ACATGGG160100.023.0865123
GAGTACT187100.022.98322312-13
CAGAGTA264800.022.76161210-11
GTGATCG22300.021.9175538
TGATCGC22150.021.6416349
Produced by FastQC (version 0.11.5)