##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4534063
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55567953069907	34.0	31.0	34.0	31.0	34.0
2	32.626542022023074	34.0	31.0	34.0	31.0	34.0
3	32.744824013252575	34.0	31.0	34.0	31.0	34.0
4	36.19627473195675	37.0	37.0	37.0	35.0	37.0
5	36.21753270741937	37.0	37.0	37.0	35.0	37.0
6	36.29499876821297	37.0	37.0	37.0	35.0	37.0
7	36.27746901620026	37.0	37.0	37.0	35.0	37.0
8	36.29286999320477	37.0	37.0	37.0	35.0	37.0
9	38.11340336470843	39.0	39.0	39.0	37.0	39.0
10-11	38.07793010375021	39.0	38.0	39.0	36.0	39.0
12-13	38.04955941282686	39.0	38.0	39.0	35.0	39.0
14-15	39.50346951509054	41.0	39.0	41.0	37.0	41.0
16-17	39.500988517362906	41.0	39.0	41.0	36.5	41.0
18-19	39.547393033577166	41.0	39.5	41.0	37.0	41.0
20-21	39.57449102493724	41.0	40.0	41.0	37.0	41.0
22-23	39.52969235319404	41.0	39.5	41.0	36.5	41.0
24-25	39.50625167757924	41.0	39.0	41.0	36.0	41.0
26-27	39.42570736224883	41.0	39.0	41.0	36.0	41.0
28-29	39.344004373119645	41.0	39.0	41.0	36.0	41.0
30-31	39.2342852315903	41.0	39.0	41.0	35.5	41.0
32-33	39.102121651154825	41.0	39.0	41.0	35.0	41.0
34-35	39.0163671082647	41.0	39.0	41.0	35.0	41.0
36-37	38.92673337357685	41.0	39.0	41.0	35.0	41.0
38-39	38.81339694662381	40.5	38.0	41.0	35.0	41.0
40-41	38.70966581187778	40.0	38.0	41.0	35.0	41.0
42-43	38.596366658337125	40.0	38.0	41.0	35.0	41.0
44-45	38.417334187901666	40.0	38.0	41.0	35.0	41.0
46-47	38.14334847133796	40.0	38.0	41.0	34.0	41.0
48-49	37.87940385918766	40.0	37.5	41.0	33.5	41.0
50-51	37.693744992074436	40.0	37.0	41.0	33.0	41.0
52-53	37.532066272568336	40.0	36.5	41.0	33.0	41.0
54-55	37.366233662831775	40.0	36.0	41.0	33.0	41.0
56-57	37.12513324142166	39.0	35.5	41.0	33.0	41.0
58-59	36.869455386923384	39.0	35.0	41.0	32.0	41.0
60-61	36.65928506066193	39.0	35.0	41.0	32.0	41.0
62-63	36.378868798250046	38.0	35.0	41.0	32.0	41.0
64-65	36.07107113421229	37.0	35.0	40.0	31.0	41.0
66-67	35.802417390318574	37.0	35.0	40.0	31.0	41.0
68-69	35.48925566318774	36.0	35.0	39.0	31.0	41.0
70-71	35.226500712495614	36.0	35.0	39.0	31.0	41.0
72-73	34.92740506693445	35.0	35.0	39.0	31.0	41.0
74-75	34.629223722740505	35.0	35.0	37.0	31.0	40.0
76-77	34.36356729052949	35.0	35.0	37.0	30.0	39.0
78-79	34.11460041468325	35.0	35.0	36.5	30.0	39.0
80-81	33.907355610188915	35.0	34.5	36.0	30.0	38.5
82-83	33.687650568595984	35.0	34.0	36.0	30.0	37.0
84-85	33.512370692687774	35.0	34.0	35.5	30.0	37.0
86-87	33.36116304515399	35.0	34.0	35.0	30.0	36.5
88-89	33.242191936900745	35.0	34.0	35.0	29.5	36.0
90-91	33.173704688267456	35.0	34.0	35.0	29.5	36.0
92-93	33.09990101593206	35.0	34.0	35.0	29.0	36.0
94-95	33.038689140402326	35.0	34.0	35.0	29.0	36.0
96-97	32.994905783179455	35.0	34.0	35.0	29.0	35.0
98-99	32.94827034825057	35.0	34.0	35.0	29.0	35.0
100	32.391911404848145	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3148922845679891
1101	2	0.28243673642607803
1101	3	0.29963741699378943
1101	4	0.1826524831874181
1101	5	0.18415336333287513
1101	6	0.14196134105029046
1101	7	0.1451806573638379
1101	8	0.186878459325996
1101	9	0.18524581232888693
1101	10-11	0.20584946808856586
1101	12-13	0.21476419162343063
1101	14-15	0.3331032524940696
1101	16-17	0.29536191396756095
1101	18-19	0.29347853360338405
1101	20-21	0.3089137974348759
1101	22-23	0.2926971236705711
1101	24-25	0.2762943689180588
1101	26-27	0.2900482301727223
1101	28-29	0.3295684340207927
1101	30-31	0.3444285183661222
1101	32-33	0.3351895565810352
1101	34-35	0.29215280832302426
1101	36-37	0.3904309101609016
1101	38-39	0.39418889353574116
1101	40-41	0.3849601005664596
1101	42-43	0.3311300217683879
1101	44-45	0.36671623617534976
1101	46-47	0.45262138168001087
1101	48-49	0.4168667733178495
1101	50-51	0.41847699524475956
1101	52-53	0.4526290064042726
1101	54-55	0.41008044875677996
1101	56-57	0.41631010071316865
1101	58-59	0.424769705290295
1101	60-61	0.3943892967330527
1101	62-63	0.410427978593809
1101	64-65	0.37858332618037593
1101	66-67	0.313095706371314
1101	68-69	0.3770834528958176
1101	70-71	0.3207560816194359
1101	72-73	0.32829618282163864
1101	74-75	0.2973152269908397
1101	76-77	0.29910983144538505
1101	78-79	0.24339991004073624
1101	80-81	0.23464282294202832
1101	82-83	0.25557006939413185
1101	84-85	0.22871435517767225
1101	86-87	0.2866815226540851
1101	88-89	0.28654785595286114
1101	90-91	0.31931185753382607
1101	92-93	0.2948535957369671
1101	94-95	0.27852960097877855
1101	96-97	0.2652577251919226
1101	98-99	0.3163813245377156
1101	100	0.24397431372728562
1102	1	0.2512052216221221
1102	2	0.2608786714714668
1102	3	0.2513177941755984
1102	4	0.17319424152322682
1102	5	0.13905277480439793
1102	6	0.14997708380947472
1102	7	0.16123278417183684
1102	8	0.20146606146261803
1102	9	0.20864800030558683
1102	10-11	0.22855381685588583
1102	12-13	0.2546211374173879
1102	14-15	0.3156609222435023
1102	16-17	0.2868892938830854
1102	18-19	0.31190938024867165
1102	20-21	0.30212602261624255
1102	22-23	0.2659722544406975
1102	24-25	0.28606732731831386
1102	26-27	0.3092908836356756
1102	28-29	0.33322433365377435
1102	30-31	0.33239080773686425
1102	32-33	0.32575680187507317
1102	34-35	0.3373853626574501
1102	36-37	0.3421277122713491
1102	38-39	0.37201404419197814
1102	40-41	0.37350821239190424
1102	42-43	0.36391852154939386
1102	44-45	0.44376438072479374
1102	46-47	0.4174632240913212
1102	48-49	0.4098172065332122
1102	50-51	0.443616449811266
1102	52-53	0.44727359863811955
1102	54-55	0.4073328917289274
1102	56-57	0.4054853175725768
1102	58-59	0.4492613506024554
1102	60-61	0.40889804662607077
1102	62-63	0.3906710549514756
1102	64-65	0.4122084904296557
1102	66-67	0.3912221695115079
1102	68-69	0.362558907381775
1102	70-71	0.3531654235650521
1102	72-73	0.30543513645928755
1102	74-75	0.2701442415958084
1102	76-77	0.2737540838531132
1102	78-79	0.27541580886820327
1102	80-81	0.32062191230874504
1102	82-83	0.28481367940465674
1102	84-85	0.27589002262132567
1102	86-87	0.29743053715836965
1102	88-89	0.32802539470561953
1102	90-91	0.2707649288066847
1102	92-93	0.24842422418872445
1102	94-95	0.30442290313202136
1102	96-97	0.2821041640660198
1102	98-99	0.19826871875110896
1102	100	0.22546889413014526
1103	1	0.2180163358139211
1103	2	0.22797508790788612
1103	3	0.2504273642261623
1103	4	0.14513879347286007
1103	5	0.1278443869363386
1103	6	0.1457723056051421
1103	7	0.18480849379280073
1103	8	0.17019310950232125
1103	9	0.18127754665439255
1103	10-11	0.2026015948341211
1103	12-13	0.2480305360056363
1103	14-15	0.2987835683131692
1103	16-17	0.30636564814065537
1103	18-19	0.3264161250986106
1103	20-21	0.27120567075323976
1103	22-23	0.30802457979895337
1103	24-25	0.29844511065169144
1103	26-27	0.2995599698327567
1103	28-29	0.3656636173308314
1103	30-31	0.38425675316424446
1103	32-33	0.330861440396383
1103	34-35	0.3515738363391563
1103	36-37	0.3526093939243822
1103	38-39	0.4206018533780096
1103	40-41	0.3513251633086796
1103	42-43	0.3784219934191597
1103	44-45	0.34323037084404007
1103	46-47	0.41566360807316727
1103	48-49	0.44034199109340477
1103	50-51	0.4663866326808872
1103	52-53	0.5221592356294096
1103	54-55	0.45421618066330893
1103	56-57	0.4698622820332119
1103	58-59	0.4548406459749543
1103	60-61	0.407268491071072
1103	62-63	0.4489698411570586
1103	64-65	0.44219471282707445
1103	66-67	0.4156376451421835
1103	68-69	0.39599547858024664
1103	70-71	0.37699699355416527
1103	72-73	0.4399205020233765
1103	74-75	0.419175657662187
1103	76-77	0.3528259820513
1103	78-79	0.3974977901169865
1103	80-81	0.37787338969313566
1103	82-83	0.3737878222036741
1103	84-85	0.39461737853038414
1103	86-87	0.4299384547813716
1103	88-89	0.38632583981897284
1103	90-91	0.38007040600002284
1103	92-93	0.3538915268477467
1103	94-95	0.3328424141828563
1103	96-97	0.3167108630404343
1103	98-99	0.3104725917597193
1103	100	0.32159760719912356
1104	1	0.19625846364038324
1104	2	0.2052196842361269
1104	3	0.22396093274088003
1104	4	0.12809190464437847
1104	5	0.09996415892340593
1104	6	0.12125019959163552
1104	7	0.1409565611026835
1104	8	0.10806839583080574
1104	9	0.17385398645494377
1104	10-11	0.1653003613856754
1104	12-13	0.1687783970888077
1104	14-15	0.24926959534933246
1104	16-17	0.2556399928743005
1104	18-19	0.2102733756162678
1104	20-21	0.22654327506607785
1104	22-23	0.23364336863154733
1104	24-25	0.22226004256310006
1104	26-27	0.26539075458762795
1104	28-29	0.3164334234382551
1104	30-31	0.2817038448759561
1104	32-33	0.28870695792415546
1104	34-35	0.23678979931731448
1104	36-37	0.27722087153818364
1104	38-39	0.2958650281661903
1104	40-41	0.30173282719923833
1104	42-43	0.29626427444452474
1104	44-45	0.31602473458210767
1104	46-47	0.32130999243523917
1104	48-49	0.25208918247467693
1104	50-51	0.19743265654742004
1104	52-53	0.20269822695738782
1104	54-55	0.24500350835215556
1104	56-57	0.29149388714781566
1104	58-59	0.2956621107397126
1104	60-61	0.2328189639581737
1104	62-63	0.2942213939593614
1104	64-65	0.30741110544423833
1104	66-67	0.26614416779786865
1104	68-69	0.2925408716965947
1104	70-71	0.2677700946751358
1104	72-73	0.18337820331092303
1104	74-75	0.1676727570137828
1104	76-77	0.1801563640822863
1104	78-79	0.1399008697426396
1104	80-81	0.12442979962290934
1104	82-83	0.11631268860600841
1104	84-85	0.15784119069556368
1104	86-87	0.18697805646421983
1104	88-89	0.1400998089110459
1104	90-91	0.0986966862657539
1104	92-93	0.1247191553440814
1104	94-95	0.1659816158834886
1104	96-97	0.13600335960416032
1104	98-99	0.14621127662302058
1104	100	0.1544686188702542
1105	1	0.1484096825570944
1105	2	0.19388298372543744
1105	3	0.16800931943902953
1105	4	0.10841252793167655
1105	5	0.08172815244368792
1105	6	0.050599950910310554
1105	7	0.07574221435082507
1105	8	0.08705480504620766
1105	9	0.07726482627960962
1105	10-11	0.07074762828765557
1105	12-13	0.10163696665254207
1105	14-15	0.1585711232360154
1105	16-17	0.15167876634099997
1105	18-19	0.14919975456002277
1105	20-21	0.13045589621444265
1105	22-23	0.17350412805886606
1105	24-25	0.1674824664964234
1105	26-27	0.13282995853776924
1105	28-29	0.16116936288897676
1105	30-31	0.1459669415557343
1105	32-33	0.17897384192953325
1105	34-35	0.14984392130110535
1105	36-37	0.13118584270821998
1105	38-39	0.18983395367263256
1105	40-41	0.14473584238074721
1105	42-43	0.1074481750528804
1105	44-45	0.14088760598454542
1105	46-47	0.14019136833461943
1105	48-49	0.19914348270317817
1105	50-51	0.13319257066043377
1105	52-53	0.11864236596429834
1105	54-55	0.12760324862415473
1105	56-57	0.16844063017511957
1105	58-59	0.15124448413541813
1105	60-61	0.1577102571534965
1105	62-63	0.16806233527116632
1105	64-65	0.191756127286709
1105	66-67	0.1298393903154178
1105	68-69	0.1576044780963315
1105	70-71	0.12183439233481153
1105	72-73	0.12103599294172795
1105	74-75	0.11663092417886389
1105	76-77	0.10090047208360176
1105	78-79	0.04388033265688307
1105	80-81	0.08825370658217224
1105	82-83	0.13557098091683883
1105	84-85	0.1691798571405414
1105	86-87	0.14443945113170997
1105	88-89	0.11596056983620429
1105	90-91	0.10565075754715991
1105	92-93	0.12060061662116084
1105	94-95	0.11312541293016665
1105	96-97	0.054361665919941515
1105	98-99	0.015492280155712024
1105	100	0.07583967267844116
1106	1	0.13348298567382244
1106	2	0.14292417809167546
1106	3	0.16884649979876087
1106	4	0.12093989092541335
1106	5	0.05769066262779887
1106	6	0.02654309802166921
1106	7	0.09518720379625734
1106	8	0.08931504147347624
1106	9	0.13171381279794758
1106	10-11	0.14072033543963158
1106	12-13	0.1203284314801607
1106	14-15	0.12741652319417085
1106	16-17	0.14577744164986228
1106	18-19	0.17488087335525648
1106	20-21	0.13748292662273798
1106	22-23	0.17118137693894653
1106	24-25	0.14697937337533062
1106	26-27	0.08267980777184647
1106	28-29	0.10878578810081052
1106	30-31	0.12919299195895206
1106	32-33	0.06408057100481557
1106	34-35	0.0806705508626564
1106	36-37	0.038325620188103926
1106	38-39	0.08105781745025098
1106	40-41	0.05326623110118334
1106	42-43	0.06233756246158606
1106	44-45	0.03112735326996585
1106	46-47	0.016803885969345345
1106	48-49	0.006706582116486004
1106	50-51	0.03539809991823262
1106	52-53	-0.02217747536077752
1106	54-55	-0.012792188408056404
1106	56-57	-0.013030710543510793
1106	58-59	0.03296307779596219
1106	60-61	0.002300418381707914
1106	62-63	-0.020657257664090878
1106	64-65	-5.051306885661688E-5
1106	66-67	-0.05496725266168312
1106	68-69	-0.08209686545433925
1106	70-71	-0.14306577116894204
1106	72-73	-0.09923755692180691
1106	74-75	-0.07390577373666929
1106	76-77	-0.06566357375828602
1106	78-79	-0.08414787606967167
1106	80-81	-0.045930453837719654
1106	82-83	-0.024639225742951965
1106	84-85	-0.05336381833352277
1106	86-87	-0.07909061197243972
1106	88-89	-0.05861794525646502
1106	90-91	8.597415560913646E-4
1106	92-93	-0.03490507714748503
1106	94-95	-0.0826611694623125
1106	96-97	-0.07536200564154427
1106	98-99	-0.03800717605601278
1106	100	-0.07627346020106529
1107	1	0.0802357534999345
1107	2	0.1055823389372037
1107	3	0.1123904639841129
1107	4	0.07069939119876523
1107	5	0.04385530429726714
1107	6	0.0755977271359285
1107	7	0.1032789841943611
1107	8	0.08109096117806303
1107	9	0.06689719602741917
1107	10-11	0.0711036949130559
1107	12-13	0.050104889316990864
1107	14-15	0.13508269868539458
1107	16-17	0.11457962904035668
1107	18-19	0.1270577800460444
1107	20-21	0.1283949859394511
1107	22-23	0.1563957528679012
1107	24-25	0.1177555935310508
1107	26-27	0.1377037213404222
1107	28-29	0.13262749706783694
1107	30-31	0.15117614086309317
1107	32-33	0.10323505153518653
1107	34-35	0.0871204726355117
1107	36-37	0.136224321198668
1107	38-39	0.14456472559319877
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2216	28-29	-0.4035782178884588
2216	30-31	-0.422517340331531
2216	32-33	-0.4258721479015293
2216	34-35	-0.4556338546542875
2216	36-37	-0.4462683150742919
2216	38-39	-0.4156023811368357
2216	40-41	-0.4144693404960762
2216	42-43	-0.4791067426662181
2216	44-45	-0.4885453391797512
2216	46-47	-0.4740454694544596
2216	48-49	-0.4810263275860365
2216	50-51	-0.44641324640807767
2216	52-53	-0.4010345243096154
2216	54-55	-0.4937979869561886
2216	56-57	-0.3922697738487173
2216	58-59	-0.386933854938448
2216	60-61	-0.39053368795611476
2216	62-63	-0.4307167364034328
2216	64-65	-0.3902536042752658
2216	66-67	-0.33552696472257537
2216	68-69	-0.39359797119567475
2216	70-71	-0.36762982503984887
2216	72-73	-0.37807395183986614
2216	74-75	-0.3556165199193302
2216	76-77	-0.3698482021277556
2216	78-79	-0.426273950870538
2216	80-81	-0.3769182359032115
2216	82-83	-0.3316320150921186
2216	84-85	-0.38531054797672937
2216	86-87	-0.369976381791588
2216	88-89	-0.3095700189779649
2216	90-91	-0.2834204796733317
2216	92-93	-0.3759559412488258
2216	94-95	-0.3339183790986908
2216	96-97	-0.32218942478040447
2216	98-99	-0.3737703080024062
2216	100	-0.43090375305006035
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	28.0
8	105.0
9	201.0
10	333.0
11	324.0
12	258.0
13	300.0
14	539.0
15	912.0
16	1745.0
17	3232.0
18	5987.0
19	9327.0
20	13467.0
21	19798.0
22	29685.0
23	35548.0
24	35050.0
25	29408.0
26	24551.0
27	25865.0
28	30657.0
29	37383.0
30	44497.0
31	57122.0
32	74724.0
33	99810.0
34	150695.0
35	251513.0
36	474732.0
37	1177954.0
38	1411285.0
39	485876.0
40	1152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.714768309962295	19.818470798109143	13.413623314223603	24.053137577704963
2	15.69505271265362	20.747595385984397	39.123193104010056	24.43415879735193
3	17.668452486109008	26.5099459578439	30.808903204263384	25.012698351783712
4	12.681694100853914	17.791900994759004	38.43852632837258	31.087878576014493
5	13.095696716880537	38.0352516452224	34.84537579205219	14.02367584584488
6	29.59292379711344	38.23164184740366	17.909482549465753	14.265951806017146
7	25.736640949471123	33.47811156488631	23.073112602587393	17.71213488305517
8	26.26692659541784	33.703986909754015	20.641111515212735	19.387974979615414
9	26.866940313798022	14.207279431273893	20.97615317652181	37.94962707840628
10-11	24.943963063592193	25.729847600264925	28.156975322133814	21.169214014009068
12-13	25.91325925555071	23.802933483720892	28.126119994362668	22.15768726636573
14-15	23.244564474223985	24.628247089804724	26.339649232696523	25.78753920327477
16-17	21.87877627637728	27.426703157852018	27.219085839786523	23.47543472598418
18-19	21.727300657269208	26.331449298344555	29.991841754294107	21.94940829009213
20-21	22.974625628272037	25.553758295815477	29.500141484580165	21.97147459133232
22-23	23.21743963440993	25.235143492337958	29.18365298938695	22.36376388386516
24-25	22.69721439688862	25.530324567611874	29.546060564222422	22.226400471277085
26-27	22.495728444884865	25.812488710456826	29.126800840658806	22.564982003999503
28-29	22.253621587000797	25.870179984305462	29.389044525637274	22.487153903056463
30-31	22.590473648863988	25.562592425544022	29.364851110099018	22.482082815492973
32-33	22.747543360553298	25.88224473882102	28.78848281154661	22.581729089079076
34-35	22.83294192065115	25.86452283676856	29.16682473151766	22.13571051106263
36-37	22.823791871575548	25.594044147284066	29.029367378120224	22.552796603020163
38-39	22.875210973895754	25.560094683926504	29.25132318747164	22.3133711547061
40-41	22.641320088804626	25.283226856297684	29.35259345063672	22.722859604260965
42-43	22.76353280751789	25.43128783466037	28.860659937270032	22.944519420551707
44-45	22.820686266249847	25.979291766595896	28.54636106149877	22.653660905655485
46-47	23.16758330555278	25.746747414257882	27.967589216387612	23.118080063801727
48-49	23.509500919159205	25.863596112513466	27.653613172372953	22.973289795954376
50-51	23.290598714741346	26.06041999290256	27.307158820554523	23.34182247180157
52-53	23.007151282352144	26.278448314649367	27.32891972936993	23.385480673628567
54-55	22.99501024979188	26.36525033279154	27.178120749815644	23.46161866760094
56-57	23.43395750765353	26.13770814464394	26.922994214952677	23.505340132749847
58-59	22.899047716513483	26.223537388198885	27.599646135902873	23.277768759384763
60-61	23.065012151036104	26.525882197643107	27.213843568409224	23.195262082911565
62-63	23.24181845811341	26.162936751229648	27.009856822327748	23.58538796832919
64-65	22.744184348694144	26.238113203427233	27.220123142189184	23.79757930568944
66-67	23.129080877734925	26.006244769236552	27.220493200682995	23.644181152345528
68-69	22.801870929755662	25.87621395332116	27.48863532474447	23.833279792178715
70-71	22.940440136043694	25.849989121047944	27.179945145379303	24.029625597529062
72-73	23.232764581601455	25.818306175748763	27.33286985643759	23.616059386212196
74-75	23.179729626155805	26.244624531929688	27.094266920972693	23.481378920941815
76-77	23.085296733557094	26.13418998953993	26.781843255514143	23.998670021388836
78-79	22.79190123565706	26.20041795093763	26.994238011479865	24.013442801925443
80-81	22.815758012986812	26.248860982587026	27.269579382635197	23.665801621790973
82-83	23.228991878353508	25.653785484784486	27.760155586962316	23.357067049899694
84-85	23.432607170000914	26.0078278663396	26.85071518714582	23.708849776513667
86-87	22.68504238748363	26.4011799572679	27.132257219656765	23.781520435591702
88-89	23.005904098837828	25.862123153156443	26.95739033516036	24.17458241284537
90-91	22.93062535743328	25.800457558706174	27.17955176185245	24.089365322008096
92-93	23.24142827305223	25.792308135109725	27.074337079127485	23.891926512710565
94-95	22.434491977725056	25.85908047594398	27.419788829577357	24.286638716753608
96-97	22.68484720697338	25.820096064394637	27.15420368845282	24.340853040179162
98-99	22.49827361432048	26.15236657646747	26.98862978461368	24.360730024598375
100	22.982928010039622	25.634252933645197	26.805902468772295	24.576916587542886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	289.5
2	443.5
3	712.0
4	485.5
5	358.0
6	766.5
7	2005.0
8	3801.5
9	4625.0
10	3407.0
11	2147.0
12	2144.5
13	2426.0
14	2801.5
15	3376.0
16	4091.0
17	4895.0
18	5759.0
19	6638.5
20	7827.5
21	9135.5
22	10698.0
23	12638.0
24	14412.5
25	16968.0
26	20678.5
27	24523.0
28	28798.5
29	36010.5
30	45119.0
31	52745.0
32	63699.5
33	72098.5
34	76221.5
35	84319.0
36	95064.5
37	109500.5
38	115915.5
39	115214.5
40	116784.0
41	116951.5
42	116193.5
43	122324.0
44	134858.5
45	154658.0
46	171275.5
47	181430.5
48	188672.5
49	184917.0
50	181490.0
51	184343.0
52	187544.0
53	190445.0
54	187823.0
55	169547.5
56	153172.5
57	136023.0
58	118159.0
59	108222.0
60	89841.0
61	68371.0
62	53272.0
63	42329.0
64	31267.5
65	21103.5
66	14724.5
67	11167.5
68	8674.5
69	6137.5
70	4584.5
71	3632.5
72	2823.5
73	2273.0
74	1787.0
75	1407.0
76	1053.5
77	731.5
78	451.5
79	290.0
80	190.5
81	75.5
82	34.5
83	18.0
84	9.5
85	5.0
86	4.0
87	1.5
88	1.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005447652580036934
2	0.09100005888758052
3	2.2055273603388396E-4
4	0.0
5	0.040780200892665146
6	0.0048301049191420585
7	4.4110547206776795E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.616582081016519E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.072712928779332E-4
24-25	0.0
26-27	0.0
28-29	2.2055273603388397E-5
30-31	1.7644218882710718E-4
32-33	0.0016431178834524353
34-35	0.0013012611425999153
36-37	7.829622129202881E-4
38-39	6.726858449033461E-4
40-41	0.0014335927842202456
42-43	5.734371136880983E-4
44-45	4.6316074567115634E-4
46-47	3.639120144559085E-4
48-49	6.17547660894875E-4
50-51	7.498793025152054E-4
52-53	0.0011248189537728082
54-55	0.001510786241832105
56-57	0.0010255702225575604
58-59	0.002624577558803219
60-61	0.0019298364402964845
62-63	0.0010145425857558663
64-65	0.004377971810272597
66-67	0.004157419074238713
68-69	0.0036942583285675566
70-71	0.0017313389778659892
72-73	0.004763939098331893
74-75	0.0034295950453268956
76-77	0.0034295950453268956
78-79	8.381003969287591E-4
80-81	3.308291040508259E-5
82-83	1.5438691522371876E-4
84-85	1.1027636801694198E-4
86-87	1.1027636801694199E-5
88-89	2.0952509923218978E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.6316074567115634E-4
98-99	5.293265664813215E-4
100	6.17547660894875E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4534063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.74073057539726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.343407323991876	27.706869134863737
2	16.65095547479308	14.566499144917197
3	7.419729787776121	9.736332047680941
4	3.9790071574769637	6.961787201311087
5	2.344309057578176	5.1270895436495225
6	1.5098045644918	3.9623972806164485
7	1.0277078187216557	3.1466883566253205
8	0.7117851203438699	2.4907200941230343
9	0.5477094717951956	2.1561491195448115
>10	2.3639906837776845	17.049648782719323
>50	0.07003305290292403	2.0678617439921094
>100	0.02856347076704243	2.0764078799337193
>500	0.0015468467528575833	0.4550148924661311
>1k	0.0011601350646424015	0.8708714400969021
>5k	9.667792205353348E-5	0.33064697436717866
>10k+	1.9335584410706697E-4	1.2950163630925282
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16036	0.35367836750393633	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14588	0.3217423313262299	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7819	0.17245018430489387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2055273603388397E-5
2	0.0	0.0	0.0	0.0	2.2055273603388397E-5
3	0.0	0.0	0.0	0.0	2.2055273603388397E-5
4	0.0	0.0	0.0	0.0	2.2055273603388397E-5
5	0.0	0.0	0.0	0.0	2.2055273603388397E-5
6	0.0	0.0	0.0	0.0	6.616582081016519E-5
7	0.0	0.0	0.0	0.0	6.616582081016519E-5
8	0.0	0.0	0.0	0.0	8.822109441355359E-5
9	0.0	0.0	0.0	0.0	1.1027636801694198E-4
10-11	0.0	0.0	0.0	0.0	1.1027636801694198E-4
12-13	0.0	0.0	0.0	0.0	1.2130400481863617E-4
14-15	0.0	0.0	0.0	0.0	1.874698256288014E-4
16-17	0.0	0.0	0.0	0.0	2.2055273603388396E-4
18-19	0.0	2.2055273603388397E-5	0.0	0.0	3.3082910405082594E-4
20-21	0.0	2.2055273603388397E-5	0.0	2.2055273603388397E-5	3.5288437765421436E-4
22-23	0.0	2.2055273603388397E-5	0.0	1.4335927842202458E-4	3.8596728805929693E-4
24-25	0.0	2.2055273603388397E-5	0.0	3.9699492486099114E-4	4.300778352660737E-4
26-27	0.0	2.2055273603388397E-5	0.0	0.0011468742273761966	4.521331088694621E-4
28-29	0.0	2.2055273603388397E-5	0.0	0.00425666780545396	4.7418838247285054E-4
30-31	0.0	2.2055273603388397E-5	0.0	0.013486799808472005	4.852160192745447E-4
32-33	0.0	2.2055273603388397E-5	0.0	0.03579570905829937	5.513818400847099E-4
34-35	0.0	2.2055273603388397E-5	0.0	0.07053276498363609	6.17547660894875E-4
36-37	0.0	2.2055273603388397E-5	0.0	0.12493209732639357	6.17547660894875E-4
38-39	0.0	2.2055273603388397E-5	0.0	0.195740553230072	6.506305712999577E-4
40-41	0.0	2.2055273603388397E-5	0.0	0.27027635037272313	6.837134817050403E-4
42-43	0.0	2.2055273603388397E-5	0.0	0.3474256974373757	6.947411185067345E-4
44-45	0.0	2.2055273603388397E-5	0.0	0.44241776084716955	7.498793025152054E-4
46-47	0.0	2.2055273603388397E-5	0.0	0.5542379980163488	7.829622129202881E-4
48-49	0.0	2.2055273603388397E-5	0.0	0.6734908623898697	8.601556705321474E-4
50-51	0.0	2.2055273603388397E-5	0.0	0.7956770781526414	8.601556705321474E-4
52-53	0.0	2.2055273603388397E-5	0.0	0.9149299425261626	9.814596753507836E-4
54-55	0.0	2.2055273603388397E-5	0.0	1.0392444921916613	0.0011248189537728082
56-57	0.0	2.2055273603388397E-5	0.0	1.1880514231937227	0.0011248189537728082
58-59	0.0	2.2055273603388397E-5	0.0	1.3500363801738087	0.0011358465905745023
60-61	0.0	2.2055273603388397E-5	0.0	1.5131461561076676	0.0011468742273761966
62-63	0.0	2.2055273603388397E-5	0.0	1.6740614323179894	0.0012571505953931385
64-65	0.0	2.2055273603388397E-5	0.0	1.841505069514914	0.0012571505953931385
66-67	0.0	2.2055273603388397E-5	0.0	2.03199646762738	0.0012571505953931385
68-69	0.0	2.2055273603388397E-5	0.0	2.237044346318082	0.0012571505953931385
70-71	0.0	2.2055273603388397E-5	0.0	2.4569464517806656	0.0013453716898066922
72-73	0.0	2.2055273603388397E-5	0.0	2.6764625899551904	0.0015438691522371877
74-75	0.0	2.2055273603388397E-5	0.0	2.8989231071557677	0.0015438691522371877
76-77	0.0	2.2055273603388397E-5	0.0	3.1401416345560262	0.0015438691522371877
78-79	0.0	2.2055273603388397E-5	0.0	3.4022575336954954	0.0015438691522371877
80-81	0.0	2.2055273603388397E-5	0.0	3.6779705090114536	0.001554896789038882
82-83	0.0	2.2055273603388397E-5	0.0	3.9669497313998505	0.0016541455202541296
84-85	0.0	2.2055273603388397E-5	0.0	4.2506908263074425	0.0016541455202541296
86-87	0.0	2.2055273603388397E-5	0.0	4.549341286170924	0.001665173157055824
88	0.0	2.2055273603388397E-5	0.0	4.786964803973831	0.0016982560674609064
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8330	0.0	58.711014	1
GTATCAA	16475	0.0	46.57053	1
ATCAACG	20385	0.0	36.770935	3
CAACGCA	20735	0.0	36.12718	5
TATCAAC	21785	0.0	35.130413	2
AACGCAG	22855	0.0	33.33525	6
TCAACGC	23335	0.0	32.34369	4
ACGCAGA	24480	0.0	30.562948	7
CGCAGAG	25060	0.0	29.855587	8
GTGGTAT	2125	0.0	29.239101	1
GCAGAGT	27190	0.0	27.292074	9
GTACATG	15445	0.0	24.868597	1
TACATGG	15545	0.0	24.22253	2
AGAGTAC	25410	0.0	23.303947	10-11
GTACTAG	2055	0.0	23.134418	1
ACATGGG	16010	0.0	23.086512	3
GAGTACT	18710	0.0	22.983223	12-13
CAGAGTA	26480	0.0	22.761612	10-11
GTGATCG	2230	0.0	21.917553	8
TGATCGC	2215	0.0	21.641634	9
>>END_MODULE