FastQCFastQC Report
Mon 16 Jan 2017
ERR1146575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535712
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.039.2241361
GTATCAA90050.035.9219361
ATCAACG92700.031.93643
CAACGCA93500.031.4621095
TATCAAC110600.028.8495962
GTACATG199900.027.8009551
AACGCAG112650.027.3225256
TACATGG202550.026.65712
GTACTAG27900.026.6261221
ACATGGG204150.025.8496993
ACGCAGA115700.025.2617247
GCATCGT24800.025.2014248
CGCAGAG116200.025.032818
TCAACGC119750.024.7223744
GTGATCG19150.023.8028018
GGGCACC28500.023.75019894
CATGGGG129050.023.304894
TGATCGC20450.022.5194559
CATCGTC28700.022.2680479
GCAGAGT129050.021.811929
Produced by FastQC (version 0.11.5)