##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146575_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4535712
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65897327696291	33.0	31.0	34.0	30.0	34.0
2	31.72784250851906	33.0	31.0	34.0	29.0	34.0
3	31.89817431089099	34.0	31.0	34.0	30.0	34.0
4	35.36479917596179	37.0	35.0	37.0	33.0	37.0
5	35.33872322581328	37.0	35.0	37.0	33.0	37.0
6	35.409636678872026	37.0	35.0	37.0	33.0	37.0
7	35.270776671887454	37.0	35.0	37.0	32.0	37.0
8	35.22196338744612	37.0	35.0	37.0	33.0	37.0
9	36.83691380757861	39.0	38.0	39.0	33.0	39.0
10-11	36.83003627655371	39.0	37.5	39.0	33.0	39.0
12-13	36.7981927644436	39.0	38.0	39.0	33.0	39.0
14-15	38.22608953125771	41.0	39.0	41.0	33.0	41.0
16-17	38.250695260192884	41.0	39.0	41.0	33.0	41.0
18-19	38.30915014004417	41.0	38.5	41.0	33.0	41.0
20-21	38.4179056121729	41.0	39.0	41.0	34.0	41.0
22-23	38.453980543738226	41.0	39.0	41.0	34.0	41.0
24-25	38.52511656383827	41.0	39.0	41.0	34.0	41.0
26-27	38.56931061760535	41.0	39.0	41.0	34.0	41.0
28-29	38.54925588308958	41.0	39.0	41.0	33.0	41.0
30-31	38.54547103078855	40.0	39.0	41.0	33.0	41.0
32-33	38.565391277047574	40.0	38.5	41.0	33.0	41.0
34-35	38.5147624011401	40.0	38.0	41.0	33.0	41.0
36-37	38.43423799835615	40.0	38.0	41.0	33.0	41.0
38-39	38.39895897270374	40.0	38.0	41.0	33.0	41.0
40-41	38.34212588894533	40.0	38.0	41.0	33.0	41.0
42-43	38.23602369374423	40.0	38.0	41.0	33.0	41.0
44-45	38.114310388313896	40.0	38.0	41.0	33.0	41.0
46-47	38.02663947799155	40.0	38.0	41.0	33.0	41.0
48-49	37.92611049819742	40.0	37.5	41.0	32.5	41.0
50-51	37.80869431304281	40.0	37.0	41.0	32.0	41.0
52-53	37.6981836589272	40.0	37.0	41.0	32.0	41.0
54-55	37.54787936271086	40.0	36.0	41.0	32.0	41.0
56-57	37.429636515722336	40.0	36.0	41.0	32.0	41.0
58-59	37.22252239560184	39.5	35.5	41.0	31.0	41.0
60-61	37.06801027049336	39.0	35.0	41.0	31.0	41.0
62-63	36.8505250112882	39.0	35.0	41.0	31.0	41.0
64-65	36.62536157498536	38.5	35.0	41.0	31.0	41.0
66-67	36.39035591325022	38.0	35.0	40.0	31.0	41.0
68-69	36.1133145137963	37.0	35.0	40.0	31.0	41.0
70-71	35.87287133750996	37.0	35.0	40.0	30.0	41.0
72-73	35.56782264835157	36.0	35.0	39.0	30.0	41.0
74-75	35.23844558472848	36.0	35.0	39.0	30.0	41.0
76-77	34.91239820782272	35.0	34.5	38.5	30.0	40.0
78-79	34.614958687853196	35.0	34.0	37.0	30.0	39.0
80-81	34.31906754661672	35.0	34.0	37.0	29.0	39.0
82-83	34.02990710168547	35.0	34.0	36.5	29.0	39.0
84-85	33.78607415991139	35.0	34.0	36.0	29.0	37.0
86-87	33.566262584573266	35.0	34.0	36.0	29.0	37.0
88-89	33.415176161978536	35.0	34.0	35.5	29.0	37.0
90-91	33.236547757000444	35.0	34.0	35.0	29.0	36.0
92-93	33.13063649102941	35.0	34.0	35.0	29.0	36.0
94-95	33.038698885643534	35.0	34.0	35.0	28.5	36.0
96-97	32.96663467609936	35.0	34.0	35.0	28.0	36.0
98-99	32.90474626695875	35.0	34.0	35.0	28.0	35.0
100	32.04249189542899	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24117021745101042
1101	2	0.2695904553066093
1101	3	0.3275168943444946
1101	4	0.272085466681915
1101	5	0.2629912265925256
1101	6	0.249579868784501
1101	7	0.2624580736543365
1101	8	0.316686138295708
1101	9	0.43388293622289353
1101	10-11	0.4156693773308646
1101	12-13	0.4166817649959853
1101	14-15	0.5062175857401598
1101	16-17	0.4357940218746066
1101	18-19	0.43059607691073154
1101	20-21	0.384334541782124
1101	22-23	0.3567965614763864
1101	24-25	0.41560428325391996
1101	26-27	0.39416669265627746
1101	28-29	0.4125736074583912
1101	30-31	0.4256027756654177
1101	32-33	0.40914804383881886
1101	34-35	0.4196135328311712
1101	36-37	0.44919503120677007
1101	38-39	0.4479469648359924
1101	40-41	0.39944132726016335
1101	42-43	0.3520068889027641
1101	44-45	0.3121121853217588
1101	46-47	0.28238663691409016
1101	48-49	0.3322041300146381
1101	50-51	0.38307269329089166
1101	52-53	0.3647958763682766
1101	54-55	0.39423093891103633
1101	56-57	0.3253568638057942
1101	58-59	0.3229787090327463
1101	60-61	0.3292293230403516
1101	62-63	0.36155415971519034
1101	64-65	0.3305888113281199
1101	66-67	0.3011130564472282
1101	68-69	0.2415163864719574
1101	70-71	0.245693332179151
1101	72-73	0.22344551321170059
1101	74-75	0.26840073097842776
1101	76-77	0.22523917429587925
1101	78-79	0.23166466883984782
1101	80-81	0.212412559673389
1101	82-83	0.23200345062381444
1101	84-85	0.2497407778724181
1101	86-87	0.28526838738548577
1101	88-89	0.26375827979350674
1101	90-91	0.24166128430187683
1101	92-93	0.21180273328093335
1101	94-95	0.2348304401403496
1101	96-97	0.20431095677371758
1101	98-99	0.1814576366565177
1101	100	0.16552569754681912
1102	1	0.18941817925681548
1102	2	0.19184106819223246
1102	3	0.26426424178862007
1102	4	0.1545266672357215
1102	5	0.16640468119508967
1102	6	0.16580219781818784
1102	7	0.21974960498415186
1102	8	0.21741707551804978
1102	9	0.2624426597232059
1102	10-11	0.31536850100805935
1102	12-13	0.2861508754585813
1102	14-15	0.3765888027574249
1102	16-17	0.4167330139319745
1102	18-19	0.30012055086712053
1102	20-21	0.30337739507378103
1102	22-23	0.30023602919303016
1102	24-25	0.3293494900690277
1102	26-27	0.3279956019024013
1102	28-29	0.33281753326240704
1102	30-31	0.3334234549473578
1102	32-33	0.3192648088784722
1102	34-35	0.3089857420327178
1102	36-37	0.33776399061142115
1102	38-39	0.34177879465325134
1102	40-41	0.3102239070920163
1102	42-43	0.301921953389801
1102	44-45	0.2676538005700877
1102	46-47	0.28415074162966647
1102	48-49	0.3183942459030149
1102	50-51	0.2411855332412145
1102	52-53	0.19588508711144215
1102	54-55	0.25180508990289496
1102	56-57	0.24854006253411853
1102	58-59	0.212375199654943
1102	60-61	0.21274810243945552
1102	62-63	0.21629064571355627
1102	64-65	0.17724939882969437
1102	66-67	0.19242338978832407
1102	68-69	0.2485602086711225
1102	70-71	0.19876590871176347
1102	72-73	0.18688673549668522
1102	74-75	0.18986702770196473
1102	76-77	0.14656528484166387
1102	78-79	0.15818874993491505
1102	80-81	0.21096567448506676
1102	82-83	0.15533303339729088
1102	84-85	0.18649495688111273
1102	86-87	0.1832509645465379
1102	88-89	0.15189532048400878
1102	90-91	0.20370945474552116
1102	92-93	0.15094814535827084
1102	94-95	0.15459063862295608
1102	96-97	0.1442229878606085
1102	98-99	0.14680509729441837
1102	100	0.0518131307725227
1103	1	0.21068731909264926
1103	2	0.24915412122902936
1103	3	0.3134816292739906
1103	4	0.2518581801822677
1103	5	0.2623423067804609
1103	6	0.2938257259351005
1103	7	0.313784667373767
1103	8	0.2818013005276683
1103	9	0.39323564845436465
1103	10-11	0.3696497245694772
1103	12-13	0.34538736918168667
1103	14-15	0.4617648154127636
1103	16-17	0.42428639814948355
1103	18-19	0.39528652424996835
1103	20-21	0.35401777097884946
1103	22-23	0.40970643230521375
1103	24-25	0.3899806120152931
1103	26-27	0.3430251577020087
1103	28-29	0.37935408204341314
1103	30-31	0.3646313026790011
1103	32-33	0.36029183383428176
1103	34-35	0.40524707985105124
1103	36-37	0.4320039457898588
1103	38-39	0.39260506764600933
1103	40-41	0.36142320409104656
1103	42-43	0.3458918451146076
1103	44-45	0.3636049839296618
1103	46-47	0.31719785330960093
1103	48-49	0.3327942764620886
1103	50-51	0.30780068629277935
1103	52-53	0.3359261522018855
1103	54-55	0.34020005895219896
1103	56-57	0.31834032940445667
1103	58-59	0.30740248367537504
1103	60-61	0.2546335345355004
1103	62-63	0.24253280676408906
1103	64-65	0.21734273025101203
1103	66-67	0.2213414998541552
1103	68-69	0.23504990889674104
1103	70-71	0.15328446592632616
1103	72-73	0.18070714646349018
1103	74-75	0.20369487573766065
1103	76-77	0.2017318236503698
1103	78-79	0.195247021722615
1103	80-81	0.22271112248830605
1103	82-83	0.17157635819381767
1103	84-85	0.22971325894577177
1103	86-87	0.19618315490172478
1103	88-89	0.2009535263646285
1103	90-91	0.2528538673259817
1103	92-93	0.1478929787305674
1103	94-95	0.11272820288272811
1103	96-97	0.15285025151623444
1103	98-99	0.11113139282419837
1103	100	0.09598830053437979
1104	1	0.20368512676743578
1104	2	0.20224284390337743
1104	3	0.26644285494068853
1104	4	0.21406543217806728
1104	5	0.2337247534344371
1104	6	0.18976108166562966
1104	7	0.15482491149257527
1104	8	0.20056346551299242
1104	9	0.31930863352140904
1104	10-11	0.33306122830148155
1104	12-13	0.26929306094843497
1104	14-15	0.34587281206475495
1104	16-17	0.3234338993243
1104	18-19	0.2749522559952453
1104	20-21	0.2506736689649216
1104	22-23	0.22706366809755707
1104	24-25	0.2197256412327917
1104	26-27	0.2369969036851316
1104	28-29	0.23311466011598014
1104	30-31	0.21421070138384835
1104	32-33	0.292955564216534
1104	34-35	0.2760094263921147
1104	36-37	0.30246746767767263
1104	38-39	0.2277379645769173
1104	40-41	0.23950069144575536
1104	42-43	0.24199459024983838
1104	44-45	0.20065662582762656
1104	46-47	0.18333541589434788
1104	48-49	0.19576751914674873
1104	50-51	0.20724222389988967
1104	52-53	0.17281704870587333
1104	54-55	0.24425082588069813
1104	56-57	0.14712306787358642
1104	58-59	0.16553174671490467
1104	60-61	0.1940715634249628
1104	62-63	0.15386644359696788
1104	64-65	0.1259591933559605
1104	66-67	0.11023627249384305
1104	68-69	0.11830769883640357
1104	70-71	0.1019440558857525
1104	72-73	0.14206365342627691
1104	74-75	0.16444132368518893
1104	76-77	0.13496573481713625
1104	78-79	0.10588118163888538
1104	80-81	0.10233812347592419
1104	82-83	0.0941175203194149
1104	84-85	0.08014263770157726
1104	86-87	0.07380047722316618
1104	88-89	0.09744782897668358
1104	90-91	0.10547038641627893
1104	92-93	0.1505344050696209
1104	94-95	0.09019482090787534
1104	96-97	0.12917672046733486
1104	98-99	0.05633425557967797
1104	100	0.036831587275770516
1105	1	0.180318260461533
1105	2	0.19553509546759784
1105	3	0.16437359911999394
1105	4	0.18171184837282084
1105	5	0.1560012654492624
1105	6	0.09817697811621429
1105	7	0.14522202649442306
1105	8	0.09258661222171582
1105	9	0.18950336026973247
1105	10-11	0.19379205056105064
1105	12-13	0.19596892732513282
1105	14-15	0.24514720445908722
1105	16-17	0.2058375365078291
1105	18-19	0.12334602880968504
1105	20-21	0.19793080716650735
1105	22-23	0.18515358427398354
1105	24-25	0.2139043732271162
1105	26-27	0.23826628494519042
1105	28-29	0.24767333138609615
1105	30-31	0.24152690589939851
1105	32-33	0.1848634529048212
1105	34-35	0.17317313448334914
1105	36-37	0.17765738847515422
1105	38-39	0.2012057427012337
1105	40-41	0.18868656997405253
1105	42-43	0.2079482297439057
1105	44-45	0.11945909472687788
1105	46-47	0.09276822621119862
1105	48-49	0.08928346519451225
1105	50-51	0.10340450214761887
1105	52-53	0.08815760408798923
1105	54-55	0.13049260881573588
1105	56-57	0.11236130257906041
1105	58-59	0.14593007413988346
1105	60-61	0.06525237017099528
1105	62-63	0.12322942606985521
1105	64-65	0.14474930992300727
1105	66-67	0.12829075038467153
1105	68-69	0.13811246167887248
1105	70-71	0.14516275431650882
1105	72-73	0.12862379001629165
1105	74-75	0.1289691181501169
1105	76-77	0.10119466520821874
1105	78-79	0.10741550875129491
1105	80-81	0.11188472123359361
1105	82-83	0.16006326215679678
1105	84-85	0.12435266244971643
1105	86-87	0.13133800558347986
1105	88-89	0.10386398401312391
1105	90-91	0.14213250018987367
1105	92-93	0.12416755631406318
1105	94-95	0.10975507305109744
1105	96-97	0.08201753950719848
1105	98-99	0.09568393413726994
1105	100	0.06551124452766999
1106	1	0.12316492579205374
1106	2	0.15541597821745867
1106	3	0.10917918760125289
1106	4	0.10186186569656996
1106	5	0.1549748654722407
1106	6	0.15117549319062817
1106	7	0.0736242303027197
1106	8	0.10064232778586302
1106	9	0.17779065509838432
1106	10-11	0.17753030949274518
1106	12-13	0.22004853128583335
1106	14-15	0.24419084886314124
1106	16-17	0.24166068759807047
1106	18-19	0.21425796408419728
1106	20-21	0.1585745440862354
1106	22-23	0.2291138635022918
1106	24-25	0.18620462432908624
1106	26-27	0.16911691966122078
1106	28-29	0.2092126575973836
1106	30-31	0.19599200451502696
1106	32-33	0.18332821159619073
1106	34-35	0.15248167256459055
1106	36-37	0.1257060598380022
1106	38-39	0.10169743998235958
1106	40-41	0.13547711884594804
1106	42-43	0.09064289366965994
1106	44-45	0.07454351770532242
1106	46-47	0.11553488036521031
1106	48-49	0.09956572799200103
1106	50-51	0.12880446053392092
1106	52-53	0.08874110334978269
1106	54-55	0.19101292947321014
1106	56-57	0.12117140989148822
1106	58-59	0.16993959548570103
1106	60-61	0.07981803294211431
1106	62-63	0.06938180129954219
1106	64-65	0.07827605801906401
1106	66-67	0.0945428807419475
1106	68-69	0.11952187238617284
1106	70-71	0.05894383080740084
1106	72-73	0.08993039963399951
1106	74-75	0.11222175643111854
1106	76-77	0.13386683871926408
1106	78-79	0.12091488091352431
1106	80-81	0.10747215462929915
1106	82-83	0.06738599436991421
1106	84-85	0.16017897420344696
1106	86-87	0.12397721541353235
1106	88-89	0.11079401908196473
1106	90-91	0.10664807536601018
1106	92-93	0.10796937261992667
1106	94-95	0.07652987264273037
1106	96-97	0.043116182565547945
1106	98-99	-0.006717535428514054
1106	100	0.0061488099970574694
1107	1	0.07229670936222377
1107	2	0.06703535786790127
1107	3	0.10278598957568263
1107	4	0.11277345165783004
1107	5	0.14590976606992712
1107	6	0.04188505631574202
1107	7	-0.029273974395906066
1107	8	0.026048578492137153
1107	9	0.11085353130876285
1107	10-11	0.1352072637101358
1107	12-13	0.08128928352018505
1107	14-15	0.11871230675085087
1107	16-17	0.10662354538004593
1107	18-19	0.12120979244659225
1107	20-21	0.1330905376814968
1107	22-23	0.12317905272858809
1107	24-25	0.1590143726847728
1107	26-27	0.15724649887351205
1107	28-29	0.15324996033955784
1107	30-31	0.12624077011132329
1107	32-33	0.13691235522880163
1107	34-35	0.14309350864218828
1107	36-37	0.14542821976294817
1107	38-39	0.12813005528400367
1107	40-41	0.10873039727654543
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2216	32-33	-0.3815595520021162
2216	34-35	-0.4090866703317104
2216	36-37	-0.38232268183924134
2216	38-39	-0.3758015782814681
2216	40-41	-0.35423882418599817
2216	42-43	-0.3327759468277236
2216	44-45	-0.31696134181698454
2216	46-47	-0.29470681801063137
2216	48-49	-0.28263755193449924
2216	50-51	-0.25194063274584266
2216	52-53	-0.23255816732496726
2216	54-55	-0.2042631980505476
2216	56-57	-0.19272849663077807
2216	58-59	-0.2075296646477085
2216	60-61	-0.2440607459291968
2216	62-63	-0.2019110902419925
2216	64-65	-0.23015187680780258
2216	66-67	-0.25557916919584756
2216	68-69	-0.2997857984689176
2216	70-71	-0.28344344837847757
2216	72-73	-0.20200300470186505
2216	74-75	-0.22584762902786082
2216	76-77	-0.20895029075494875
2216	78-79	-0.21837070016047022
2216	80-81	-0.1691808346784356
2216	82-83	-0.1989655555490515
2216	84-85	-0.17659509813000085
2216	86-87	-0.15699885604653474
2216	88-89	-0.19656804100889502
2216	90-91	-0.14633247181633635
2216	92-93	-0.17666759922944664
2216	94-95	-0.16158155579918088
2216	96-97	-0.15651881529117873
2216	98-99	-0.15028774692307678
2216	100	-0.13059116218724398
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	152.0
1	47.0
2	56.0
3	65.0
4	63.0
5	64.0
6	171.0
7	848.0
8	2404.0
9	2826.0
10	2752.0
11	3162.0
12	3851.0
13	4952.0
14	6446.0
15	7353.0
16	8041.0
17	9280.0
18	10512.0
19	11863.0
20	13294.0
21	15071.0
22	17621.0
23	21230.0
24	27317.0
25	36804.0
26	43514.0
27	39871.0
28	38303.0
29	49597.0
30	57846.0
31	59714.0
32	73228.0
33	98877.0
34	153476.0
35	237036.0
36	434067.0
37	1001009.0
38	1448142.0
39	593510.0
40	1277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95819854258328	21.422287286518806	13.17904976712747	23.440464403770434
2	14.511945666417434	21.560785166307518	41.447282979103875	22.479986188171175
3	17.305859612898903	28.133294936921544	30.9517488521869	23.609096597992654
4	11.405435023477388	18.361731472488074	41.72730510019922	28.505528403835317
5	12.058942275104965	40.0153997145408	36.03515562553733	11.890502384816905
6	27.65743747329167	40.18070596868604	19.814107819405162	12.347748738617124
7	25.191360094753477	33.746446689371695	24.466311649787823	16.595881566087005
8	24.606863013571775	36.2086209017776	21.7373742184334	17.447141866217226
9	26.174126400215904	15.477253023566757	21.9615722811712	36.387048295046135
10-11	23.575370043455017	27.46109779787998	29.800317702190082	19.163214456474922
12-13	24.26824964757868	25.585087741250266	29.039274182408448	21.107388428762608
14-15	21.89861205296718	26.58960467990166	26.75819615064175	24.75358711648941
16-17	21.168635228838117	29.279802259964864	27.612594461493007	21.938968049704012
18-19	20.870407874152903	29.420959155257208	28.92261779423616	20.78601517635373
20-21	22.640312344796378	28.41316782689845	27.982872410149334	20.96364741815584
22-23	22.525663262977588	28.377619213818594	27.479903844479047	21.616813678724764
24-25	21.897190456005898	28.553865624081897	28.16852964570234	21.380414274209862
26-27	21.91001153132972	28.74481586252362	27.56842179531559	21.77675081083107
28-29	21.462569037415197	29.150199001059	27.75064310712852	21.63658885439728
30-31	22.001630769597128	28.69875566723304	27.779364105693976	21.520249457475852
32-33	22.06802034698895	28.972299337859614	27.231533489084807	21.728146826066627
34-35	22.17072691547731	28.86006611910656	27.911045480534373	21.058161484881754
36-37	21.947723281163636	28.646976850494564	27.74393675996012	21.661363108381682
38-39	22.035748957923886	28.559396271184013	28.036795306536593	21.36805946435551
40-41	22.020431378096486	28.025893314613075	28.347635753731915	21.606039553558524
42-43	21.706558567173026	28.272050294315605	28.009336239587807	22.012054898923562
44-45	21.773197404121674	28.8287319720564	27.933413299476733	21.46465732434519
46-47	21.795471497046293	28.375414782694225	27.729471923131722	22.099641797127763
48-49	22.11810431277291	28.11728300445346	28.02828220630233	21.736330476471302
50-51	22.00555730031388	28.09975307748903	27.78278538119931	22.11190424099778
52-53	21.67424977309124	28.48979387499072	27.694623538766578	22.141332813151458
54-55	21.67578166741821	28.478039139529816	27.664123773891518	22.18205541916046
56-57	22.363800019541664	28.080287557982604	27.578468264539403	21.97744415793633
58-59	21.859711075476813	28.222362862637507	28.156880155949853	21.761045905935823
60-61	21.94621639489612	28.607957318064997	27.67735571385327	21.768470573185613
62-63	22.079427954464578	28.204564679712522	27.399723147569315	22.31628421825358
64-65	21.52396132593517	28.271069571059115	27.706106043583528	22.498863059422185
66-67	22.158370346125665	27.9934927474924	27.542094010213457	22.306042896168478
68-69	21.75603696569351	28.07907664947866	27.762680944652367	22.40220544017546
70-71	21.842099865215108	27.931251545306228	27.73807310321311	22.48857548626555
72-73	22.149526815132475	27.947637692727252	27.92087361820954	21.981961873930732
74-75	22.16539184289231	28.141315759292095	27.76361061853214	21.929681779283452
76-77	21.939174370839797	28.124065042031134	27.581890049549397	22.35487053757967
78-79	21.88876126099302	28.12528810012497	27.647642905381414	22.338307733500603
80-81	21.845549153602214	28.191279999161843	27.930272196057476	22.032898651178463
82-83	22.27867532725328	27.62844760087782	28.5443377187662	21.548539353102704
84-85	22.523578676696086	28.019210306898007	27.501591540689684	21.955619475716226
86-87	21.61990940146944	28.315983079187756	27.91174235603156	22.152365163311245
88-89	22.36654107915718	27.62570136417213	27.77887864876706	22.22887890790363
90-91	22.265949402697228	27.59851876014035	27.94302468949971	22.19250714766271
92-93	22.425651143843826	27.677577767397178	27.901470149079266	21.995300939679733
94-95	21.640817049421713	27.857837379493834	28.18061343219933	22.320732138885123
96-97	21.82043140176074	27.82845160831442	27.93307929947526	22.41803769044958
98-99	21.58503490627519	28.226986173123215	27.797392532670536	22.390586387931062
100	22.064955720829957	27.743398703073296	27.392746843471656	22.79889873262509
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1604.0
1	1098.5
2	632.0
3	625.0
4	536.5
5	474.5
6	452.5
7	441.0
8	461.0
9	502.0
10	455.0
11	412.0
12	412.0
13	436.0
14	472.0
15	587.5
16	794.5
17	996.5
18	1341.5
19	1786.5
20	2478.0
21	3682.0
22	5652.5
23	8492.0
24	11809.0
25	16212.0
26	22395.5
27	29097.5
28	37344.0
29	49046.0
30	62254.5
31	75026.0
32	90719.0
33	105599.5
34	117483.0
35	128730.5
36	141697.0
37	158828.0
38	165976.0
39	163695.0
40	160526.0
41	157329.0
42	157083.0
43	159107.0
44	164775.5
45	176870.0
46	185204.5
47	189589.0
48	189501.0
49	179647.5
50	170371.0
51	170197.0
52	168628.0
53	159814.0
54	144527.5
55	121746.0
56	105493.0
57	85226.5
58	64969.5
59	58135.5
60	47492.5
61	33185.0
62	23637.5
63	18735.0
64	13270.5
65	6625.5
66	3932.0
67	2608.0
68	1669.5
69	1218.5
70	1030.0
71	824.0
72	626.0
73	489.5
74	364.5
75	264.5
76	206.0
77	165.5
78	124.0
79	99.5
80	77.5
81	61.0
82	48.5
83	41.0
84	38.0
85	31.0
86	25.0
87	20.0
88	15.5
89	13.5
90	12.5
91	9.0
92	10.5
93	9.0
94	6.0
95	5.5
96	4.5
97	7.0
98	9.0
99	9.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06085042436556819
2	0.010340162691105607
3	0.012478746445982462
4	0.010957485836843256
5	0.012412604680367713
6	0.013448825674998766
7	0.0033732300463521496
8	0.0059527589053273225
9	0.007496066769671444
10-11	0.0073307123556345735
12-13	0.0066692946994870925
14-15	0.01077008416760147
16-17	0.010240950042683486
18-19	0.008091342660204176
20-21	0.007485043142068985
22-23	0.003152757494302989
24-25	0.0041779548613315835
26-27	0.005335435759589674
28-29	0.006812601858319047
30-31	0.009480319738113883
32-33	0.010924414954035884
34-35	0.012412604680367713
36-37	0.019037804869444973
38-39	0.01972126978079737
40-41	0.019258277421494132
42-43	0.024781114850325595
44-45	0.0285071009799564
46-47	0.03108662983893157
48-49	0.03269607946889044
50-51	0.03298269378655435
52-53	0.030877180914484874
54-55	0.037535451986369504
56-57	0.03988348466569306
58-59	0.037270884923910515
60-61	0.037689782772803916
62-63	0.0336771823255092
64-65	0.03558426990073444
66-67	0.03734805031712772
68-69	0.040401595163008584
70-71	0.04014805172815205
72-73	0.0363559238329065
74-75	0.03862679111901285
76-77	0.038615767491410394
78-79	0.03743623933794738
80-81	0.0427606514699346
82-83	0.038847263671062005
84-85	0.034911828616984504
86-87	0.03378741860153378
88-89	0.031384267784197936
90-91	0.033897654877558366
92-93	0.03558426990073444
94-95	0.03888033455386938
96-97	0.03394174938796819
98-99	0.03146143317741514
100	0.028661431766390813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4535712.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.846767214744986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12152454353583	37.219706416240555
2	15.176903270087697	16.344543548502283
3	6.276540827607158	10.139142985740165
4	3.241727413123023	6.982261655523716
5	1.8478281409063342	4.974978587811918
6	1.15146304344885	3.720153747418321
7	0.7405925095420004	2.791495872360433
8	0.5343462339894307	2.301825381896362
9	0.3867797356921396	1.8744154552075707
>10	1.4959590828937053	12.52797011540584
>50	0.022001909730978358	0.767844717989283
>100	0.00433328944291482	0.3556615159036436
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2047255204916008E-5	0.0
2	0.0	0.0	0.0	2.2047255204916008E-5	0.0
3	0.0	0.0	0.0	2.2047255204916008E-5	0.0
4	0.0	0.0	0.0	6.614176561474802E-5	0.0
5	0.0	0.0	0.0	6.614176561474802E-5	0.0
6	0.0	0.0	0.0	8.818902081966403E-5	0.0
7	0.0	0.0	0.0	8.818902081966403E-5	0.0
8	0.0	0.0	0.0	8.818902081966403E-5	0.0
9	0.0	0.0	0.0	1.5433078643441205E-4	0.0
10-11	0.0	0.0	0.0	2.7559069006145013E-4	0.0
12-13	0.0	0.0	0.0	3.4173245567619814E-4	0.0
14-15	0.0	0.0	0.0	3.7480333848357217E-4	0.0
16-17	0.0	1.1023627602458004E-5	0.0	3.8582696608603016E-4	0.0
18-19	0.0	2.2047255204916008E-5	0.0	4.1889784889340414E-4	0.0
20-21	0.0	2.2047255204916008E-5	0.0	4.5196873170077817E-4	0.0
22-23	0.0	2.2047255204916008E-5	0.0	6.834649113523963E-4	0.0
24-25	0.0	2.2047255204916008E-5	0.0	0.0011685045258605486	1.1023627602458004E-5
26-27	0.0	2.2047255204916008E-5	0.0	0.0018740166924178607	2.2047255204916008E-5
28-29	0.0	2.2047255204916008E-5	0.0	0.005258270366372468	2.2047255204916008E-5
30-31	0.0	2.2047255204916008E-5	0.0	0.01523465334659696	2.2047255204916008E-5
32-33	0.0	2.2047255204916008E-5	0.0	0.03597009686682047	2.2047255204916008E-5
34-35	0.0	2.2047255204916008E-5	0.0	0.06878743623933795	2.2047255204916008E-5
36-37	0.0	2.2047255204916008E-5	0.0	0.1193307687966079	2.2047255204916008E-5
38-39	0.0	2.2047255204916008E-5	0.0	0.19031190692883498	2.2047255204916008E-5
40-41	0.0	2.2047255204916008E-5	0.0	0.26467729873501666	2.2047255204916008E-5
42-43	0.0	2.2047255204916008E-5	0.0	0.34179859744181285	2.2047255204916008E-5
44-45	0.0	2.2047255204916008E-5	0.0	0.4350695105862101	2.2047255204916008E-5
46-47	0.0	2.2047255204916008E-5	0.0	0.5454380701420196	2.2047255204916008E-5
48-49	0.0	2.2047255204916008E-5	0.0	0.6660916742509224	2.2047255204916008E-5
50-51	0.0	2.2047255204916008E-5	0.0	0.788012995534108	2.2047255204916008E-5
52-53	0.0	2.2047255204916008E-5	0.0	0.9039815579119662	2.2047255204916008E-5
54-55	0.0	2.2047255204916008E-5	0.0	1.0280414629500285	2.2047255204916008E-5
56-57	0.0	2.2047255204916008E-5	0.0	1.1712935036439704	2.2047255204916008E-5
58-59	0.0	2.2047255204916008E-5	0.0	1.3360305945351028	2.2047255204916008E-5
60-61	0.0	2.2047255204916008E-5	0.0	1.5028731982983046	2.2047255204916008E-5
62-63	0.0	2.2047255204916008E-5	0.0	1.6651740674892939	2.2047255204916008E-5
64-65	0.0	2.2047255204916008E-5	0.0	1.8336261208824545	2.2047255204916008E-5
66-67	0.0	2.2047255204916008E-5	0.0	2.0194844822598967	2.2047255204916008E-5
68-69	0.0	2.2047255204916008E-5	0.0	2.223432616532972	2.2047255204916008E-5
70-71	0.0	2.2047255204916008E-5	0.0	2.444114617506579	2.2047255204916008E-5
72-73	0.0	2.2047255204916008E-5	0.0	2.6642895316104727	2.2047255204916008E-5
74-75	0.0	2.2047255204916008E-5	0.0	2.880573105170699	2.2047255204916008E-5
76-77	0.0	2.2047255204916008E-5	0.0	3.11446141201205	2.2047255204916008E-5
78-79	0.0	2.2047255204916008E-5	0.0	3.3673103583296298	2.2047255204916008E-5
80-81	0.0	2.2047255204916008E-5	0.0	3.6408947481674323	2.2047255204916008E-5
82-83	0.0	2.2047255204916008E-5	0.0	3.921578354181218	2.2047255204916008E-5
84-85	0.0	2.2047255204916008E-5	0.0	4.203265110306827	2.2047255204916008E-5
86-87	0.0	2.2047255204916008E-5	0.0	4.495336123633952	2.2047255204916008E-5
88	0.0	2.2047255204916008E-5	0.0	4.724572459627066	2.2047255204916008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5430	0.0	39.224136	1
GTATCAA	9005	0.0	35.921936	1
ATCAACG	9270	0.0	31.9364	3
CAACGCA	9350	0.0	31.462109	5
TATCAAC	11060	0.0	28.849596	2
GTACATG	19990	0.0	27.800955	1
AACGCAG	11265	0.0	27.322525	6
TACATGG	20255	0.0	26.6571	2
GTACTAG	2790	0.0	26.626122	1
ACATGGG	20415	0.0	25.849699	3
ACGCAGA	11570	0.0	25.261724	7
GCATCGT	2480	0.0	25.201424	8
CGCAGAG	11620	0.0	25.03281	8
TCAACGC	11975	0.0	24.722374	4
GTGATCG	1915	0.0	23.802801	8
GGGCACC	2850	0.0	23.750198	94
CATGGGG	12905	0.0	23.30489	4
TGATCGC	2045	0.0	22.519455	9
CATCGTC	2870	0.0	22.268047	9
GCAGAGT	12905	0.0	21.81192	9
>>END_MODULE