FastQCFastQC Report
Mon 16 Jan 2017
ERR1146575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535712
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170580.3760820792854573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153130.33760961895287883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88470.19505206679789192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102500.059.592451
GTATCAA196500.044.0412451
ATCAACG240100.035.187163
CAACGCA244950.034.6056185
TATCAAC253700.033.997272
AACGCAG263900.032.482976
TCAACGC268850.031.6516884
ACGCAGA284900.029.7085887
CGCAGAG285650.029.614138
TAGTCCG804.7746746E-429.3838235
GTACATG197600.027.5541381
GCAGAGT306800.027.3734729
TACATGG199350.027.1446132
ACATGGG196500.026.820783
GTACTAG26900.026.2409381
GTGGTAT26550.024.814411
GGGCACC31350.024.73594
GTGATCG16950.024.6763558
CATGGGG127050.023.3867954
AGAGTAC295800.023.2040710-11
Produced by FastQC (version 0.11.5)