##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4535712
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54422216401747	34.0	31.0	34.0	31.0	34.0
2	32.614555774264325	34.0	31.0	34.0	31.0	34.0
3	32.732346101339765	34.0	31.0	34.0	31.0	34.0
4	36.18586343224614	37.0	37.0	37.0	35.0	37.0
5	36.21409384017328	37.0	37.0	37.0	35.0	37.0
6	36.29700254337136	37.0	37.0	37.0	35.0	37.0
7	36.27568064286269	37.0	37.0	37.0	35.0	37.0
8	36.293235108401944	37.0	37.0	37.0	35.0	37.0
9	38.110501284032146	39.0	39.0	39.0	37.0	39.0
10-11	38.075627266457836	39.0	38.0	39.0	36.0	39.0
12-13	38.05119593131134	39.0	38.0	39.0	35.0	39.0
14-15	39.4861764812228	41.0	39.0	41.0	36.5	41.0
16-17	39.488065490048754	41.0	39.0	41.0	36.5	41.0
18-19	39.55263837298312	41.0	39.5	41.0	37.0	41.0
20-21	39.58868431240784	41.0	40.0	41.0	37.0	41.0
22-23	39.54312707685144	41.0	39.5	41.0	36.5	41.0
24-25	39.52307884627595	41.0	39.0	41.0	36.0	41.0
26-27	39.44334825491566	41.0	39.0	41.0	36.0	41.0
28-29	39.3599396302058	41.0	39.0	41.0	36.0	41.0
30-31	39.24579459630594	41.0	39.0	41.0	35.5	41.0
32-33	39.10904263762778	41.0	39.0	41.0	35.0	41.0
34-35	39.02024202594873	41.0	39.0	41.0	35.0	41.0
36-37	38.933645037427425	41.0	39.0	41.0	35.0	41.0
38-39	38.83062482803141	41.0	38.0	41.0	35.0	41.0
40-41	38.73382778712582	40.0	38.0	41.0	35.0	41.0
42-43	38.630593168172936	40.0	38.0	41.0	35.0	41.0
44-45	38.44903578534087	40.0	38.0	41.0	35.0	41.0
46-47	38.17692657734882	40.0	38.0	41.0	34.0	41.0
48-49	37.9279970818253	40.0	38.0	41.0	33.5	41.0
50-51	37.772061916629625	40.0	38.0	41.0	33.0	41.0
52-53	37.64879924033977	40.0	37.0	41.0	33.0	41.0
54-55	37.5297570701138	40.0	37.0	41.0	33.0	41.0
56-57	37.339968895732355	40.0	36.5	41.0	33.0	41.0
58-59	37.14175944151657	40.0	36.0	41.0	32.0	41.0
60-61	36.98620922139678	39.0	35.5	41.0	32.0	41.0
62-63	36.756142806245194	39.0	35.0	41.0	32.0	41.0
64-65	36.493718516519564	39.0	35.0	41.0	31.0	41.0
66-67	36.2681970327922	38.0	35.0	41.0	31.0	41.0
68-69	35.98706509584383	37.0	35.0	40.0	31.0	41.0
70-71	35.742672704968925	37.0	35.0	40.0	31.0	41.0
72-73	35.43853478792305	36.5	35.0	39.0	31.0	41.0
74-75	35.11025722091702	36.0	35.0	39.0	31.0	41.0
76-77	34.79152931226674	35.5	35.0	39.0	30.0	40.5
78-79	34.49202330306686	35.0	35.0	37.0	30.0	39.0
80-81	34.21953763819219	35.0	35.0	37.0	30.0	39.0
82-83	33.940399434532	35.0	35.0	36.5	30.0	39.0
84-85	33.706978419264715	35.0	34.0	36.0	30.0	37.0
86-87	33.498073400603914	35.0	34.0	36.0	29.5	37.0
88-89	33.336039192964634	35.0	34.0	35.5	29.0	37.0
90-91	33.22571605075454	35.0	34.0	35.0	29.0	36.0
92-93	33.12255848695861	35.0	34.0	35.0	29.0	36.0
94-95	33.04030183133321	35.0	34.0	35.0	29.0	36.0
96-97	32.98540879579656	35.0	34.0	35.0	29.0	36.0
98-99	32.93229210761177	35.0	34.0	35.0	29.0	35.5
100	32.40003333544987	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2484544885482194
1101	2	0.22694881215794993
1101	3	0.2271771884222673
1101	4	0.12422484218269858
1101	5	0.13001519071167422
1101	6	0.11755025795937257
1101	7	0.15198265070365835
1101	8	0.15672293750917277
1101	9	0.21002584812233493
1101	10-11	0.24480328972313004
1101	12-13	0.23738337441785262
1101	14-15	0.3416197028183703
1101	16-17	0.33646494951229045
1101	18-19	0.30699299791999834
1101	20-21	0.337299965567432
1101	22-23	0.3584795113120478
1101	24-25	0.32895263496030935
1101	26-27	0.3428787518870351
1101	28-29	0.3621637444184529
1101	30-31	0.39346561649649914
1101	32-33	0.3741666986697396
1101	34-35	0.3834312037061167
1101	36-37	0.3621119672944957
1101	38-39	0.3944044046329225
1101	40-41	0.37532630474751016
1101	42-43	0.3397428200564434
1101	44-45	0.3702777799299568
1101	46-47	0.41281314321668106
1101	48-49	0.3910277090555141
1101	50-51	0.38106982072033446
1101	52-53	0.4000026152925855
1101	54-55	0.3830760563510438
1101	56-57	0.40113772611180565
1101	58-59	0.363698321057754
1101	60-61	0.3244171032445422
1101	62-63	0.3342887869008564
1101	64-65	0.359089638967518
1101	66-67	0.3281226408359075
1101	68-69	0.32688611167678516
1101	70-71	0.35759280416650796
1101	72-73	0.3655177741120994
1101	74-75	0.40173172801005563
1101	76-77	0.3402314442266814
1101	78-79	0.2734923677370844
1101	80-81	0.2940126308814044
1101	82-83	0.3044657655327967
1101	84-85	0.28179493537799516
1101	86-87	0.2927074283351345
1101	88-89	0.24656212155885981
1101	90-91	0.25040408096126043
1101	92-93	0.2397338516603611
1101	94-95	0.25249636486675087
1101	96-97	0.2800730439654018
1101	98-99	0.296041704022727
1101	100	0.22962035577034356
1102	1	0.21466587935059067
1102	2	0.2427046875275849
1102	3	0.2319394689864609
1102	4	0.1404618637169932
1102	5	0.10184252943984262
1102	6	0.10669706571608373
1102	7	0.1750566773208604
1102	8	0.14614860293060872
1102	9	0.21540071291686758
1102	10-11	0.20152266979295774
1102	12-13	0.20978515189820968
1102	14-15	0.2680039532243299
1102	16-17	0.24296834240418264
1102	18-19	0.23714519621392327
1102	20-21	0.2533595271658413
1102	22-23	0.27182041933578915
1102	24-25	0.2529570279345279
1102	26-27	0.2798067957594057
1102	28-29	0.2970256727799381
1102	30-31	0.29438318526199225
1102	32-33	0.23824938322855616
1102	34-35	0.24093380874242598
1102	36-37	0.26226639465683377
1102	38-39	0.32537104288832097
1102	40-41	0.313367924555358
1102	42-43	0.2958179464937132
1102	44-45	0.2872658006114861
1102	46-47	0.2919923335167809
1102	48-49	0.30490713602140573
1102	50-51	0.27074294333915816
1102	52-53	0.25620765583111904
1102	54-55	0.32131603882761794
1102	56-57	0.3116124527696229
1102	58-59	0.2958634043006825
1102	60-61	0.2798605108882981
1102	62-63	0.28079326233299184
1102	64-65	0.27546451900168734
1102	66-67	0.2282618594662793
1102	68-69	0.21834038701053515
1102	70-71	0.209965754299148
1102	72-73	0.23382976900715846
1102	74-75	0.18027345605647582
1102	76-77	0.18340892298801492
1102	78-79	0.17701670388752433
1102	80-81	0.1608571377022585
1102	82-83	0.15585387299065445
1102	84-85	0.15633346860152386
1102	86-87	0.185221832658101
1102	88-89	0.19883401399529532
1102	90-91	0.12018113733405755
1102	92-93	0.115716225234614
1102	94-95	0.18868326126439428
1102	96-97	0.12487309659007906
1102	98-99	0.09635320172826312
1102	100	0.10435057465831221
1103	1	0.21124745327749395
1103	2	0.20784084892074617
1103	3	0.22556358116339936
1103	4	0.1596433730347968
1103	5	0.14908578646231518
1103	6	0.11559932252095706
1103	7	0.12647355659268555
1103	8	0.13337958001761763
1103	9	0.15227192298184633
1103	10-11	0.1531693348160985
1103	12-13	0.16865231703459926
1103	14-15	0.25761462343859876
1103	16-17	0.2816384483299643
1103	18-19	0.2392648616039068
1103	20-21	0.23872568056629007
1103	22-23	0.27387116974451686
1103	24-25	0.23009758340972297
1103	26-27	0.23848758965643668
1103	28-29	0.2475676236894202
1103	30-31	0.25173282799019603
1103	32-33	0.24833515222713487
1103	34-35	0.2972840671862045
1103	36-37	0.2880883684275304
1103	38-39	0.29588799592925596
1103	40-41	0.29500518626192473
1103	42-43	0.30948864288649247
1103	44-45	0.3077784314636318
1103	46-47	0.3584810806975369
1103	48-49	0.3627935569607459
1103	50-51	0.36320114027610373
1103	52-53	0.36268882903169697
1103	54-55	0.3305483051922664
1103	56-57	0.3631581768298915
1103	58-59	0.2590865267097229
1103	60-61	0.2596701994485926
1103	62-63	0.28584739311712326
1103	64-65	0.32020148440198426
1103	66-67	0.25119891669197614
1103	68-69	0.2419376115282006
1103	70-71	0.26774322407590034
1103	72-73	0.29666689639840627
1103	74-75	0.3041484238030492
1103	76-77	0.2820943167488892
1103	78-79	0.2633499874053058
1103	80-81	0.26473198809629395
1103	82-83	0.22087100157142459
1103	84-85	0.22351339555667948
1103	86-87	0.23255834938645137
1103	88-89	0.2646680762627298
1103	90-91	0.26398003218728405
1103	92-93	0.21337226536230247
1103	94-95	0.27161100970749885
1103	96-97	0.23991937512627715
1103	98-99	0.22770588204984676
1103	100	0.21785794950960735
1104	1	0.1965334202825133
1104	2	0.15226790655103883
1104	3	0.2093422502113711
1104	4	0.09627234535281559
1104	5	0.07345303156306926
1104	6	0.0775479539199111
1104	7	0.09010367519380225
1104	8	0.08453187813984897
1104	9	0.10974226448626467
1104	10-11	0.15456425124407502
1104	12-13	0.17544759588273706
1104	14-15	0.22563120575608053
1104	16-17	0.2492903042075767
1104	18-19	0.21174722494283316
1104	20-21	0.18861065843252334
1104	22-23	0.21562903502075415
1104	24-25	0.19491408999964932
1104	26-27	0.17850489411696913
1104	28-29	0.23438878216349224
1104	30-31	0.2291502463367152
1104	32-33	0.23781041590935814
1104	34-35	0.27065868291730055
1104	36-37	0.2704446666918727
1104	38-39	0.2790791021006598
1104	40-41	0.2675410275123937
1104	42-43	0.25750390546826907
1104	44-45	0.2579716970943622
1104	46-47	0.28974071486725705
1104	48-49	0.31123729448623294
1104	50-51	0.29328044964803723
1104	52-53	0.267467193050166
1104	54-55	0.2791828207401892
1104	56-57	0.280411924717761
1104	58-59	0.2801061083801386
1104	60-61	0.24551335670969365
1104	62-63	0.25342928147020416
1104	64-65	0.28509852660173607
1104	66-67	0.29867169083929923
1104	68-69	0.32722632396878026
1104	70-71	0.2633683625872365
1104	72-73	0.24793728508238644
1104	74-75	0.18256461404173052
1104	76-77	0.17075493405086917
1104	78-79	0.15317745732125587
1104	80-81	0.23299289717942884
1104	82-83	0.22314400405739576
1104	84-85	0.16764910835816949
1104	86-87	0.1995439059021038
1104	88-89	0.25552730579661187
1104	90-91	0.19786998629005836
1104	92-93	0.20149021006746892
1104	94-95	0.22704278739034578
1104	96-97	0.19359683648292503
1104	98-99	0.14954673144511332
1104	100	0.13912081247021746
1105	1	0.21647048162108717
1105	2	0.14734420199349074
1105	3	0.1810476808741015
1105	4	0.12317888073214789
1105	5	0.0738980697495677
1105	6	0.10757254748578049
1105	7	0.11222427893222431
1105	8	0.10129087734922138
1105	9	0.10499631758352734
1105	10-11	0.11198186027588264
1105	12-13	0.1434288909685506
1105	14-15	0.21017766799500492
1105	16-17	0.19899763470891685
1105	18-19	0.153519074280986
1105	20-21	0.16125379829394149
1105	22-23	0.18460844577881375
1105	24-25	0.18674981026083515
1105	26-27	0.23654311727248967
1105	28-29	0.22904839101400398
1105	30-31	0.22378295270399207
1105	32-33	0.20734988135581744
1105	34-35	0.2028362614190229
1105	36-37	0.1898710048482286
1105	38-39	0.18145394866257902
1105	40-41	0.127687562722123
1105	42-43	0.1762030849331424
1105	44-45	0.16512458569113164
1105	46-47	0.1833620300924963
1105	48-49	0.19192137324510838
1105	50-51	0.22264638991813968
1105	52-53	0.23387951652895111
1105	54-55	0.1484336929844403
1105	56-57	0.22140179065296195
1105	58-59	0.16881500333131783
1105	60-61	0.18071046724612927
1105	62-63	0.20911702728577097
1105	64-65	0.19627602669042687
1105	66-67	0.18428219794829204
1105	68-69	0.2456888420873682
1105	70-71	0.19983667919058234
1105	72-73	0.19507330172977788
1105	74-75	0.07909012277506378
1105	76-77	0.10042302744813725
1105	78-79	0.13510018276258506
1105	80-81	0.15390876075665005
1105	82-83	0.13883262792707995
1105	84-85	0.0987397862841064
1105	86-87	0.08260362823412493
1105	88-89	0.07619953887553521
1105	90-91	0.06950186918641066
1105	92-93	0.044016665338851624
1105	94-95	0.04296236471784454
1105	96-97	0.04545715192010391
1105	98-99	0.06704294725192028
1105	100	0.13876811487704543
1106	1	0.1276315974872091
1106	2	0.13484153486506045
1106	3	0.13186801196430764
1106	4	0.08703279394833174
1106	5	0.06320926661152271
1106	6	0.06540552421216006
1106	7	0.05487551840042215
1106	8	0.08473404756198732
1106	9	0.06624706757201437
1106	10-11	0.05732966822449015
1106	12-13	0.09292012483014389
1106	14-15	0.1611330664706827
1106	16-17	0.09123778273604444
1106	18-19	0.11651574673957299
1106	20-21	0.12550765014226783
1106	22-23	0.14983573100160186
1106	24-25	0.12043394155592324
1106	26-27	0.12927899242669127
1106	28-29	0.1338478304754318
1106	30-31	0.17820594312547655
1106	32-33	0.12963789635693246
1106	34-35	0.11408355634350897
1106	36-37	0.11358915635254618
1106	38-39	0.2052872630191871
1106	40-41	0.20631462352250907
1106	42-43	0.18326865893804722
1106	44-45	0.20410021822465296
1106	46-47	0.19913051648821778
1106	48-49	0.2162170875715006
1106	50-51	0.2701181280426965
1106	52-53	0.24762547142134395
1106	54-55	0.2654588260451902
1106	56-57	0.21082719197247002
1106	58-59	0.25106097284177764
1106	60-61	0.24720468403486962
1106	62-63	0.23651249860046164
1106	64-65	0.3001135207695995
1106	66-67	0.28846857574866647
1106	68-69	0.2703367664783798
1106	70-71	0.1926937199087746
1106	72-73	0.21662485919895857
1106	74-75	0.21437802824900132
1106	76-77	0.21642752109594454
1106	78-79	0.20575643259611098
1106	80-81	0.18436320630503644
1106	82-83	0.20586067822924292
1106	84-85	0.20583591167007143
1106	86-87	0.22080185480113812
1106	88-89	0.1926823122782153
1106	90-91	0.1846588971931311
1106	92-93	0.19087986359027553
1106	94-95	0.16398706252464734
1106	96-97	0.1720930214896157
1106	98-99	0.1420701652800176
1106	100	0.21605757779339996
1107	1	0.06642649958003233
1107	2	0.052357786728428835
1107	3	0.08346836148585624
1107	4	0.06008395633831043
1107	5	0.05213635966975261
1107	6	0.044248715872782896
1107	7	0.056034827323657055
1107	8	0.0774280495025721
1107	9	0.07754733544066283
1107	10-11	0.08219118475084741
1107	12-13	0.04876011817737691
1107	14-15	0.09893658461541577
1107	16-17	0.08239670000737931
1107	18-19	0.1070779502359116
1107	20-21	0.10010133424115963
1107	22-23	0.07724549121791568
1107	24-25	0.09698936218232745
1107	26-27	0.086889472065522
1107	28-29	0.15646290224479742
1107	30-31	0.08348558450948929
1107	32-33	0.0504215077679433
1107	34-35	0.1067267063180779
1107	36-37	0.15786430431582232
1107	38-39	0.13773675248090456
1107	40-41	0.1035807822297059
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2216	28-29	-0.4077085163986851
2216	30-31	-0.37899491712981614
2216	32-33	-0.43513050954645394
2216	34-35	-0.44616384212737614
2216	36-37	-0.438268319083015
2216	38-39	-0.37899048329330043
2216	40-41	-0.3980698015990001
2216	42-43	-0.37400395461099833
2216	44-45	-0.3435882258655454
2216	46-47	-0.34085952364771543
2216	48-49	-0.3555314720546576
2216	50-51	-0.3216215304450998
2216	52-53	-0.32166458747900606
2216	54-55	-0.37380331864808625
2216	56-57	-0.2914652791392385
2216	58-59	-0.28744775987598814
2216	60-61	-0.27263494627762697
2216	62-63	-0.325002955830044
2216	64-65	-0.2795951355133326
2216	66-67	-0.23748163296471603
2216	68-69	-0.3004052147025078
2216	70-71	-0.275983134051927
2216	72-73	-0.2414999089955785
2216	74-75	-0.2418082772535186
2216	76-77	-0.2026545130269639
2216	78-79	-0.34419014814066884
2216	80-81	-0.2589999654956401
2216	82-83	-0.26662627323942445
2216	84-85	-0.29690109034390133
2216	86-87	-0.2575165521255869
2216	88-89	-0.22123711898236564
2216	90-91	-0.19729000642801964
2216	92-93	-0.2423233430766203
2216	94-95	-0.2288583235448911
2216	96-97	-0.24311297191165693
2216	98-99	-0.22290943105761585
2216	100	-0.28850799749633893
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	23.0
8	125.0
9	215.0
10	344.0
11	313.0
12	238.0
13	290.0
14	499.0
15	935.0
16	1930.0
17	3668.0
18	6694.0
19	10937.0
20	15654.0
21	23342.0
22	34195.0
23	41419.0
24	38684.0
25	30325.0
26	23869.0
27	23862.0
28	28432.0
29	34077.0
30	41269.0
31	52972.0
32	68537.0
33	91509.0
34	139648.0
35	223563.0
36	385104.0
37	963460.0
38	1527091.0
39	719969.0
40	2520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37274271081519	21.08515686977474	13.589089252633386	22.95301116677669
2	14.806180886070585	21.6472903455873	41.22621615800698	22.320312610335137
3	17.03602531031594	28.54137178384814	31.5164803668673	22.906122538968628
4	11.333678152404739	18.72312439590521	41.184537289845565	28.75866016184449
5	11.584084736484337	39.621946544765926	36.08828216910508	12.70568654964466
6	27.038016067549236	40.50087785694868	19.28921698864309	13.171889086859
7	24.035222710446654	34.63149099038519	24.468826269757106	16.864460029411052
8	24.937055086389964	35.22547286952963	21.58163922224339	18.25583282183701
9	26.399207004324786	14.39006709420704	22.092407983575676	37.1183179178925
10-11	23.530263826274684	27.05642465835573	29.673621252848502	19.73969026252108
12-13	24.897281838000296	25.3121560628188	29.354972273371853	20.435589825809046
14-15	22.132560914926017	25.907492783380597	28.085254990893375	23.874691310800007
16-17	20.282394473017685	28.704291630509164	28.83701610684276	22.176297789630382
18-19	19.969841559605193	27.69081237962199	31.852948335344045	20.486397725428777
20-21	21.65709595318221	26.72117409570978	31.361437851433248	20.26029209967476
22-23	21.44065496582987	26.750082181596746	30.951242887791857	20.858019964781523
24-25	20.915128650143572	26.884092288046507	31.606702541960335	20.594076519849587
26-27	20.929316058867936	27.17951448416478	30.96292268997679	20.928246766990497
28-29	20.421596572356194	27.508237132859335	31.298128485052125	20.772037809732343
30-31	21.04937148145972	26.932160829224916	31.198498227012312	20.819969462303053
32-33	21.225622730606936	27.321976731342	30.604564438581725	20.847836099469337
34-35	21.24909232875453	27.33004596589394	31.119528631591685	20.301333073759842
36-37	21.105058714057257	27.11744696509456	30.846401142411533	20.93109317843665
38-39	21.209130775895034	26.969215032437866	31.143172515910823	20.67848167575628
40-41	21.12189362680198	26.659684917326903	31.287879205447528	20.93054225042359
42-43	21.123038894647557	26.814638745651003	30.693829397627763	21.368492962073677
44-45	21.21695827455409	27.520040796479385	30.369036830265237	20.893964098701286
46-47	21.50642454125262	27.146587396172162	29.701918791470366	21.64506927110485
48-49	21.944936530946332	27.294371841015945	29.48113566337976	21.279555964657963
50-51	21.773195635357954	27.403415433930405	29.04537593132489	21.77801299938675
52-53	21.35765781725418	27.87735251588554	28.94081652473367	21.82417314212661
54-55	21.418772041435886	27.861532462885286	28.79336480780469	21.92633068787414
56-57	22.166860960223563	27.42951002196482	28.587929547227116	21.81569947058451
58-59	21.49545753544441	27.618576949826906	29.247307157604794	21.638658357123887
60-61	21.679771664696517	27.969476214943946	28.756294218826774	21.59445790153277
62-63	21.79056065147855	27.62080291874244	28.469071260317147	22.119565169461854
64-65	21.264822657271885	27.619671270902472	28.726561970324095	22.388944101501547
66-67	21.713910642812127	27.43066859088706	28.658180385850212	22.197240380450605
68-69	21.429176174885075	27.32389677106415	28.903793365743958	22.34313368830682
70-71	21.46643435775976	27.152251810802362	28.872829613983953	22.508484217453926
72-73	21.704145750940974	27.075670113503296	29.07664553762207	22.143538597933663
74-75	21.860473063846865	27.419105553032274	28.638398073878747	22.082023309242118
76-77	21.63122533718366	27.35530826212271	28.41166364148981	22.601802759203824
78-79	21.434434479235588	27.46195765167167	28.570415962893236	22.533191906199505
80-81	21.318865037792868	27.55678520487584	28.75396258204751	22.370387175283785
82-83	21.745758044563324	26.969171041401964	29.423112580343115	21.861958333691604
84-85	22.07605120877517	27.33407382796111	28.293097415684038	22.296777547579683
86-87	21.066355418147232	27.682848400173643	28.741083812438667	22.509712369240457
88-89	21.67106510571687	27.0768128403554	28.60364442092731	22.648477633000418
90-91	21.54674944088161	27.008626209071473	28.77982552684121	22.664798823205707
92-93	21.722752679182452	27.11720894095569	28.58231519108797	22.57772318877389
94-95	20.962287729026887	27.24167672021504	28.94306340437841	22.852972146379663
96-97	21.175229322704457	27.13955429307558	28.746827800423464	22.938388583796502
98-99	20.919630852155997	27.536838665874285	28.571245893264962	22.97228458870476
100	21.305534169548267	27.170988403933617	28.156751055410485	23.366726371107635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	489.0
1	319.0
2	348.5
3	505.0
4	302.5
5	244.0
6	555.0
7	1363.0
8	2591.0
9	3242.5
10	2613.0
11	2025.0
12	2240.5
13	2649.0
14	3191.5
15	3895.5
16	4754.5
17	5740.0
18	6899.0
19	8162.0
20	9442.5
21	10970.0
22	13182.5
23	16312.5
24	19629.5
25	23759.0
26	29502.5
27	35445.0
28	42766.0
29	53627.0
30	65425.5
31	76432.5
32	90924.5
33	104236.5
34	114695.0
35	124826.0
36	137038.0
37	154084.0
38	160299.0
39	158561.5
40	157288.0
41	153876.5
42	153108.0
43	155146.0
44	160988.5
45	173394.0
46	180930.0
47	184371.5
48	184931.0
49	175511.5
50	165669.0
51	163533.0
52	161376.5
53	153520.0
54	140939.0
55	120464.0
56	103378.0
57	83568.5
58	64489.5
59	57974.0
60	47195.5
61	32324.0
62	22471.5
63	17617.5
64	12121.5
65	5877.5
66	3371.0
67	2078.5
68	1258.5
69	897.5
70	745.5
71	581.0
72	431.5
73	348.0
74	273.0
75	211.0
76	138.0
77	85.0
78	50.5
79	49.5
80	40.0
81	19.0
82	11.0
83	6.0
84	4.0
85	1.5
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006481893030245306
2	0.08960004515277867
3	2.645670624589921E-4
4	0.0
5	0.038582696608603015
6	0.005181104973155262
7	4.4094510409832016E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.2047255204916008E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.511813801229002E-4
24-25	0.0
26-27	0.0
28-29	3.307088280737401E-5
30-31	1.4330715883195406E-4
32-33	0.0018299221820080288
34-35	0.0014661424711269145
36-37	9.590556014138465E-4
38-39	8.929138357990984E-4
40-41	0.0016976386507785326
42-43	8.047248149794343E-4
44-45	5.842522629302743E-4
46-47	4.0787422129094614E-4
48-49	8.157484425818923E-4
50-51	8.047248149794343E-4
52-53	0.0013779534503072506
54-55	0.0017307095335859066
56-57	0.0010913391326433425
58-59	0.002722836017807127
60-61	0.0022377964032989747
62-63	0.0013228353122949606
64-65	0.004729136241454484
66-67	0.004310238392561079
68-69	0.0035716553431963936
70-71	0.0018629930648154027
72-73	0.005489766546024086
74-75	0.003792127895245553
76-77	0.0036267734812086834
78-79	9.480319738113883E-4
80-81	3.307088280737401E-5
82-83	1.9842529684424408E-4
84-85	1.1023627602458006E-4
86-87	2.2047255204916008E-5
88-89	2.645670624589921E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.0787422129094614E-4
98-99	4.960632421106102E-4
100	7.937011873769763E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4535712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.58455205488201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.22258680727387	32.17394715946939
2	16.252971687300786	15.792866979763767
3	6.903228642760554	10.061708140228577
4	3.6356253684274873	7.065409198576593
5	2.094857942257087	5.0888867371586235
6	1.2562093973880009	3.6619422515537563
7	0.8431843227150917	2.867601283316827
8	0.5995215815703925	2.3301989990265537
9	0.4205560507553519	1.8389274605927093
>10	1.7177926949704152	13.217082362721527
>50	0.03468997063874635	1.1297022374540837
>100	0.015341266854246735	1.3752555296017674
>500	0.001849220739690504	0.5941226360060323
>1k	0.0013208719569207986	1.0389338296796877
>5k	8.805813046138657E-5	0.3784980889135314
>10k+	1.7611626092277314E-4	1.3849171059364673
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17058	0.3760820792854573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15313	0.33760961895287883	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8847	0.19505206679789192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.2047255204916008E-5	0.0
4	0.0	0.0	0.0	4.4094510409832016E-5	0.0
5	0.0	0.0	0.0	4.4094510409832016E-5	0.0
6	0.0	0.0	0.0	4.4094510409832016E-5	0.0
7	0.0	0.0	0.0	4.4094510409832016E-5	0.0
8	0.0	0.0	0.0	4.4094510409832016E-5	0.0
9	0.0	0.0	0.0	1.1023627602458004E-4	0.0
10-11	0.0	0.0	0.0	1.5433078643441205E-4	0.0
12-13	0.0	0.0	0.0	1.7637804163932806E-4	0.0
14-15	0.0	0.0	0.0	1.9842529684424408E-4	0.0
16-17	0.0	0.0	0.0	2.314961796516181E-4	0.0
18-19	0.0	0.0	0.0	2.6456706245899214E-4	0.0
20-21	0.0	0.0	0.0	3.3070882807374014E-4	0.0
22-23	0.0	0.0	0.0	5.952758905327323E-4	0.0
24-25	0.0	0.0	0.0	0.0011244100154507164	0.0
26-27	0.0	0.0	0.0	0.0018078749268031127	0.0
28-29	0.0	0.0	0.0	0.005203152228360178	0.0
30-31	0.0	0.0	0.0	0.015168511580982213	0.0
32-33	0.0	0.0	0.0	0.035892931473603264	0.0
34-35	0.0	0.0	0.0	0.06865515270810846	0.0
36-37	0.0	0.0	0.0	0.11923155614818579	0.0
38-39	0.0	0.0	0.0	0.1906756866397161	0.0
40-41	0.0	0.0	0.0	0.26510722021151256	0.0
42-43	0.0	0.0	0.0	0.3422174952907063	0.0
44-45	0.0	0.0	0.0	0.4359293535392018	0.0
46-47	0.0	0.0	0.0	0.5467498818267121	0.0
48-49	0.0	0.0	0.0	0.6679326200605329	0.0
50-51	0.0	0.0	0.0	0.7904051227238413	0.0
52-53	0.0	0.0	0.0	0.9068256538334003	0.0
54-55	0.0	0.0	0.0	1.0316461891760325	0.0
56-57	0.0	0.0	0.0	1.1757911437057733	0.0
58-59	0.0	0.0	0.0	1.341189652253053	0.0
60-61	0.0	0.0	0.0	1.5087487918104148	0.0
62-63	0.0	0.0	0.0	1.6714685588502975	0.0
64-65	0.0	0.0	0.0	1.8406481716652205	0.0
66-67	0.0	0.0	0.0	2.027344328740449	1.1023627602458004E-5
68-69	0.0	0.0	0.0	2.23257120381541	2.2047255204916008E-5
70-71	0.0	0.0	0.0	2.453727220775922	2.2047255204916008E-5
72-73	0.0	0.0	0.0	2.6747289069500004	2.2047255204916008E-5
74-75	0.0	0.0	0.0	2.8924235048433413	2.2047255204916008E-5
76-77	0.0	0.0	0.0	3.1278220486662294	2.2047255204916008E-5
78-79	0.0	0.0	0.0	3.3819828066685007	2.2047255204916008E-5
80-81	0.0	0.0	0.0	3.6571105043706478	2.2047255204916008E-5
82-83	0.0	0.0	0.0	3.9401311194361543	2.2047255204916008E-5
84-85	0.0	0.0	0.0	4.22278795479078	2.2047255204916008E-5
86-87	0.0	0.0	0.0	4.516303063333828	2.2047255204916008E-5
88	0.0	0.0	0.0	4.746178769727884	2.2047255204916008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10250	0.0	59.59245	1
GTATCAA	19650	0.0	44.041245	1
ATCAACG	24010	0.0	35.18716	3
CAACGCA	24495	0.0	34.605618	5
TATCAAC	25370	0.0	33.99727	2
AACGCAG	26390	0.0	32.48297	6
TCAACGC	26885	0.0	31.651688	4
ACGCAGA	28490	0.0	29.708588	7
CGCAGAG	28565	0.0	29.61413	8
TAGTCCG	80	4.7746746E-4	29.383823	5
GTACATG	19760	0.0	27.554138	1
GCAGAGT	30680	0.0	27.373472	9
TACATGG	19935	0.0	27.144613	2
ACATGGG	19650	0.0	26.82078	3
GTACTAG	2690	0.0	26.240938	1
GTGGTAT	2655	0.0	24.81441	1
GGGCACC	3135	0.0	24.735	94
GTGATCG	1695	0.0	24.676355	8
CATGGGG	12705	0.0	23.386795	4
AGAGTAC	29580	0.0	23.20407	10-11
>>END_MODULE