FastQCFastQC Report
Mon 16 Jan 2017
ERR1146574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144561
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33910.29627079727511246No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26520.23170455746788507No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16160.1411895040980778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13880.12126920277730938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGT5250.027.7502658
GTACTAG5350.027.2482341
GTGATCG4800.025.4563928
CATCGTC6550.024.3950869
TTAAGGT3550.023.829174
GTCTAGG1852.8002432E-722.8771151
TGATCGC5450.022.420319
TAGGCAT7950.021.2813975
CTAGGCA9750.020.7266254
ATAAGGT6500.020.244613
GGCATCG7100.019.8576417
CAATGCG3350.019.64029318-19
TCTAACG3605.456968E-1219.581842
CATGGGG26200.019.5519454
TGCGCGA3850.019.53268694
GTATAAG9150.019.5295831
CGACGAG3500.019.46909324-25
GTATTAT3657.2759576E-1219.3254181
GTACATG56950.018.9917341
TACATGG55850.018.93322
Produced by FastQC (version 0.11.5)